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Analysis of Cyclofenil and its Metabolites in Human Urine using GC/MS  

Myung, Seung-Woon (Donping Control Center, Korea Institute of Science and Technology)
Min, Hye-Ki (Donping Control Center, Korea Institute of Science and Technology)
Cho, Hyun-Woo (Department of Chemistry, KyongGi University)
Kim, Dong-Hyun (Donping Control Center, Korea Institute of Science and Technology)
Kim, Myungsoo (Donping Control Center, Korea Institute of Science and Technology)
Publication Information
Analytical Science and Technology / v.15, no.3, 2002 , pp. 185-189 More about this Journal
Abstract
International Olympic Committee (IOC) prohibits the use of cyclofenil for male. To prove whether cyclofenil product was taken or not, the analytical method in urine using GC/MS was established. The extraction recoveries of cyclofenil and its metabolites were higher in the range of pH 5-9. Because the parent drug (cyclofenil) was not excreted in urine, metabolites were detected and theses were used as a criteria of cyclofenil dose. Therefore the metabolites were hydrolyzed, extracted at pH 9.6, derivatized with MSTFA and analyzed by GC/MS.
Keywords
cyclofenil; metabolite; doping; GC/MS;
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  • Reference
1 P. H. Slee and D. H. Stevenson, Cancer and Chemotheraphy., 42(6), 512-514(1988)
2 L. Borgstroem, Eur. J. Clin. Pharmacol., 19(3), 213-16(1981)
3 E. M. Hassan, M. A. El Sayed and E. F. Khamis, Pharma Sci., 4, 305-308(1994).
4 Doping Control Center, 'Reaccreditation for Antidoping Analysis: Test Report', KIST, Seoul, Korea, Nov. 2001.
5 R. A. Roeder, M. J. Garber and G. T. Schelling, J. Anim. Sci., 76, 142-151(1978)
6 International Olympic Committee, 'Olympic Movement Anti-Doping Code, Appendix A.', Lausanne, Switzerland, 2001
7 L. Schubert, Acta Eurpopea Fertilitatis., 8, 83(1973).