• 보존과학연구
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• s.36
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• pp.33-48
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• 2015

In this study, ancient DNA analyses were carried out on the human skeletal remains from a historical cemetery site in Myeongam-ri, Asan, Korea. Human remains of 27 individuals out of tombs from the Goryeo to Joseon Dynasty were selected for the analysis of this study. In order to identify the genealogy of the population and traditional burial pattern of the cemetery, we conducted comparative analyses of the hyper variable regions (HVRs) in mitochondrial DNA (mtDNA) of each sample. We sequenced 9 segmental amplicons of HVRs and assigned relevant haplogroups according to the sequence polymorphism on the basis of the known mtDNA database. As a result, we were analyses 18 human remains of 27 individuals and result of amelogenin analysis were only 4 samples.

• Journal of Conservation Science
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• v.34 no.1
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• pp.1-9
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• 2018

The analysis of ancient DNA extracted from archaeological bones has become an important research tool in palaeogenetics and anthropology. Eight human skeletal remains of the Joseon dynasty, excavated from Hwamyeong-dong, were used in this study. DNA was extracted from bone powder using a silica-based protocol. The isolated DNA was analyzed by the sequencing variation of hyper-variable region of the mitochondrial DNA. In the present study, 3 human remains were identified into mtDNA haplogroups including the A 5a, D4a, and M4"67+16311 groups, using HaploGrep 2 program. The identified haplotypes of the 3 samples have been confirmed that the specimens in the tombs were not related by the maternal line. This is the first analysis of human skeletal remains of the Joseon dynasty excavated in Busan. Date from the analysis of human remains from the Joseon dynasty are considered as the basis for understanding the genetic relationship between modern and ancient humans of the Korean peninsula.

• KOMUNHWA
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• no.33
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• pp.23-34
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• 1988

• 보존과학연구
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• s.29
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• pp.137-148
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• 2008

Analyses of ancient DNA (aDNA) from archaeological and historical skeletal material are characterized by low quality. Many soil contaminants such as humic acid, fulvic acid, and bone collagen are often co-extracted with aDNA and inhibit amplification by polymerase chain reaction (PCR). In this study, we compared with two methods of DNA extraction by phenolchloroform extraction and silica-bead extraction. In addition, we applied new protocol, ultra sonication based silica-bead extraction method to extract aDNA from some ancient human skeletal remains. This method was more effective by both mitochondrial DNA (mtDNA) and amelogenin gene amplification.

• Transactions on Control, Automation and Systems Engineering
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• v.4 no.1
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• pp.43-48
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• 2002

We show that humans execute the postural control ingeniously by regulating the impedance properties of the musculo-skeletal system as the motor command against the alteration of the environment. Adjusting muscle activity can control the impedance properties of the musculo-skeletal system. To quantify the changes in human arm viscoelasticity on the vertical plane during interaction with the environment, we asked our subject to hold an object. By utilizing surface electromyographic(EMG) studies, we determined a relationship between the perturbation and a time-varying muscle co-activation. Our study showed when the subject lifts the object by himself the muscle stiffness increases while the torque remains the same just before the lift-off. These results suggest that the central nervous system(CNS) simultaneously controls not only the equilibrium point(EP) and the torque, but also the muscle stiffness as themotor command in posture control during the contact task.

• Journal of Conservation Science
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• v.26 no.1
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• pp.95-107
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• 2010

Biochemical research was carried out on 4 human skeletal remains from historical lime-layered tombs assigned to the Joseon Dynasty in Oknam-ri, Seocheon. The preservation of femur was evaluated by stereoscopic microscopy and scanning electron microscopy. Most of specimens showed good histological preservation. The histological results proved to be a good potentiality for biochemical analysis using bio-molecules. The amelogenin gene and mitochondrial DNA (mtDNA) analyses revealed that three specimens perhaps have maternal consanguinity due to sharing with mtDNA haplogroup D4b1, and two specimens buried in the same tomb were a couple in Gatjaegol site. Carbon and nitrogen stable isotope analysis indicated that four deads diet were built around C3 plant as rice, barley, wheat and bean. In this study we characterized genetic and diet features from the social stratum who could make lime-layered tombs during period of the Joseon Dynasty. The results suggest that biochemical research using the human skeletal remains from the Joseon Dynasty has the great potential and reasonable value for archaeology, anthropology, and population genetics.

• 보존과학연구
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• s.32
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• pp.171-183
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• 2011

In this study molecular genetic analysis was carried out on 4 human skeletal remains from Osuri, Buyeo. We showed that real-time PCR is the method of the choice to assess the initial number of genuine ancient DNA molecules. Human mitochondrial DNA quantification was accomplished by the real-time PCR for the cytochrome b gene of the mitochondria. Histological results proved to be a good potentiality for biochemical analysis using biomolecule. The level of specimen's preservation state was proved that level of quantitative result was BO-04, BO-01, BO-03, BO-02. Continually, we showed that biochemical and biomolecule results for the level of preservation state were similar. This study will be useful to important material for predicting biochemistry and biology analysis of the ancient bone.

• Journal of Oral Medicine and Pain
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• v.21 no.2
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• pp.225-242
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• 1996

The author studied to determine the sex and age of 8 ancient human skeletal remains, which had excavated from ancient tombs located in Yeanri, Kimhae. Some kinds of personal identification methods their skulls and teeth were used for this study and the results were obtained as follows : 1. Sex determination was possible in ancient teeth from 4th to 5th century, using detection of X-Y homologous amelogenin gene by polymerase chain reaction. 2. DNA analysis proved that the materials examined were all male, but which always did not coincide with the results from other methods for sex determination including comparison of sexual differentiation of cranium and teeth and use of discriminant functions in the dental measurement. 3. There was little difference of the estimated ages between the methods by regression of pulp cavity and attrition in teeth. The ages from these two methods always did not coincide with ones from evaluation closure of cranial and palatal sutures. 4. Sex and age of the materials were determined as follows ; $\cdot$ Y9 was estimated to be male in early sixties. $\cdot$ Yl2 was estimated to be male in late twenties to early thirties. $\cdot$ Y37 was estimated to be male in early forties. $\cdot$ Y70 was estimated to be male in early sixties. $\cdot$ Y87 was estimated to be male in late forties. $\cdot$ Y109 was estimated to be male in early forties. $\cdot$ Yl29 was estimated to be male in late thirties to early forties. $\cdot$ Yl42 was estimated to be male in late fifties to early sixties.

• Journal of Conservation Science
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• v.23
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• pp.81-93
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• 2008

The analysis of ancient DNA (aDNA) in paleogenetics has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. In this study, paleogenetic analyses were carried out on the human skeletal remains from a historical cemetery site in Myeongam-ri, Asan, Korea. Archaeological records show that this particular location had been used as a habitation or mortuary site as early as the Bronze Age and up until the Joseon Dynasty. Human remains of twenty individuals out of forty-nine tombs from the Goryeo to Joseon Dynasty were selected for the analysis of this study. In order to identify the genealogy of the population and traditional burial pattern of the cemetery, we conducted comparative analyses of the hyper variable regions (HVRs) in mitochondrial DNA (mtDNA) of each sample. A number of cautious steps were taken at all experimental stages in order to avoid erroneous recombination by the segmental and modern contaminations derived from the researchers. We sequenced segmental amplicons of HVRs andassigned relevant haplogroups according to the sequence polymorphism on the basis of the known mtDNA database. The result shows that diverse haplogroups were unexpectedly present in the small population group of the Myeongam-ri site. This diversity appears to be related to the geographical conditions and archaeological properties of the Myeongam-ri site.

• Korean Journal of Heritage: History & Science
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• v.47 no.4
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• pp.92-105
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• 2014

Microbial characteristics of bacterial population were investigated in human remains and soil inside the bones in excavated grave no.4 and no.5 at Keundokgol site, Osu-ri, Buyeo. Phylogenetic characteristics of bacterial populations were analyzed by direct extracting of ancient DNA. In this study, based on the 16S rDNA sequences, in case of grave no.4, 319s from human remain were classified into 11 phyla, and 462s from soil were classified into 16 phyla. In case of grave no.5, 271s from human remain were classified into 10 phyla, and 497s from soil were classified into 11 phyla. Especially, Actinobacteria phylogenetic group are dominant group of bacterial populations in grave no.4 and no.5. Also, most of these were analyzed uncultured group. Thus, the discovery of a diversely microbial community and uncultured group was thought to be due to the specificity of the sample. Conclusively the general excavated human bones were contaminated with soil bacteria species their near around. This results contribute to preservation and management of ancient human bone from archaeological sites.