• Tuberculosis and Respiratory Diseases
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• v.40 no.6
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• pp.653-658
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• 1993

Background: Recent advancement of molecular genetics has revealed that malignant transformation of a cell may be a complex multistep process and this process is grouped, in general, into two distinct categories, activation of protooncogenes and inactivation of tumor suppressor genes. This study was focused on the mutation of p53 tumor suppressor gene, because p53 gene mutation is now generally accepted to be one of the most frequent genetic changes in a variety of human cancers. Although lung cancer is one of the common cancers in Korea, the genetic change in the carcinogenesis process is not yet known clearly. To investigate the role of p53 gene mutation in lung cancer, we examined the mutations of exon 4-8 of the p53 gene in humna lung cancer cell lines, because most of the mutations of p53 gene have been reported to develop in exon 4-8. Method: Genomic DNA was obtained by the digestion of proteinase K and the extraction by phenol-chloroform-ethanol method from two human pulmonary adenocarcinoma cell lines, PC-9 and PC-14, and one human small cell lung cancer cell line, H69. To detect the mutations of exon 4-8 of the p53 gene, polymerase chain reaction single-strand conformation polymorphism(PCR-SSCP) analysis was performed with the DNA extracted from the cells. Results: The mutation of p53 gene was present in all three cell lines tested. In PC-9, PC-14 and H69, the altered mobility was detected in exon 7, 7 and 5, respectively. Conclusion: These results suggest that p53 gene mutation plays an important role in certain steps of the carcinogenesis of human non-small cell and small cell lung cancer.

• Journal of the Ergonomics Society of Korea
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• v.31 no.2
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• pp.319-325
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• 2012

Objective: The aim of this study was to develop the assistive device for accelerator and brake pedals using bio-signals from the prefrontal lobe in the driving simulator and evaluate its performance. Background: There is lack of assistive devices for the driving in peoples with disabilities in Korea. However, if bio-signals and/or brain waves are used at driving a car, the people with serious physical limitations can improve their community mobility. Method: 15 subjects with driver's license participated in this study for experiment of driving performance evaluation in the simulator. Each subject drove the simulator the same course 10 times in three separated groups which use different interface controllers to accelerate and brake: (1) conventional pedal group, (2) joystick group and (3) bio-signal group(horizontal quick glance of the eyes and clench teeth). All experiments were recorded and the driving performances were evaluated by three inspectors. Results: Average score of bio-signal group for the driving in the simulator was increased 3% compared with the pedal group and was increased 9% compared with the joystick group(p<0.01). The subjects using bio-signals was decreased 44% in number of deduction compared with others because the device had the built-in modified cruise control. Conclusion: The assistive device for accelerator and brake pedals using bio-signals showed significantly better performance than using general pedal and a joystick interface(p<0.01). Application: This study can be used to design adaptive vehicle for driving in people with disabilities.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.783-788
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• 2003

In this paper, we present a new approach to detect and recognize human face in the image from vision camera equipped on the mobile robot platform. Due to the mobility of camera platform, obtained facial image is small and pose-various. For this condition, new algorithm should cope with these constraints and can detect and recognize face in nearly real time. In detection step, ‘coarse to fine’ detection strategy is used. Firstly, region boundary including face is roughly located by dual ellipse templates of facial color and on this region, the locations of three main facial features- two eyes and mouth-are estimated. For this, simplified facial feature maps using characteristic chrominance are made out and candidate pixels are segmented as eye or mouth pixels group. These candidate facial features are verified whether the length and orientation of feature pairs are suitable for face geometry. In recognition step, pseudo-convex hull area of gray face image is defined which area includes feature triangle connecting two eyes and mouth. And random lattice line set are composed and laid on this convex hull area, and then 2D appearance of this area is represented. From these procedures, facial information of detected face is obtained and face DB images are similarly processed for each person class. Based on facial information of these areas, distance measure of match of lattice lines is calculated and face image is recognized using this measure as a classifier. This proposed detection and recognition algorithms overcome the constraints of previous approach [15], make real-time face detection and recognition possible, and guarantee the correct recognition irregardless of some pose variation of face. The usefulness at mobile robot application is demonstrated.

• Bulletin of the Korean Chemical Society
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• v.34 no.7
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• pp.1972-1984
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• 2013

Microtubules play an important role in intracellular transport, mobility, and particularly mitosis. Paclitaxel (Taxol$^{TM}$) and paclitaxel-like compounds have been shown to be anti-tumor agents useful for various human tumors. Paclitaxel-like compounds operate by stabilizing microtubules through interface binding at the interface between two ${\beta}$-tubulin monomers in adjacent protofilaments. In this paper we present the elucidation of the structural features of paclitaxel and paclitaxel-like compounds (e.g., epothilones) with microtubule stabilizing activities, and relate their activities to spatial and chemical features of the molecules. CATALYST program was used to generate three-dimensional quantitative structure activity relationships (3D-QSARs) resulting in 3D pharmacophore models of epothilone- and paclitaxel-derivatives. Pharmacophore models were generated from diverse conformers of these compounds resulting in a high correlation between experimental and predicted biological activities (r = 0.83 and 0.91 for epothilone and paclitaxel derivatives, respectively). On the basis of biological activities of the training sets, five- and four-feature pharmacophore hypotheses were generated in the epothilone and paclitaxel series. The validation of generated hypotheses was achieved by using twelve epothilones and ten paclitaxels, respectively, which are not in the training sets. The clustering (grouping) and merging techniques were used in order to supplement spatial restrictions of each of hypothesis and to develop more comprehensive models. This approach may be of use in developing novel inhibitor candidates as well as contributing a better understanding of structural characters of many compounds useful as anticancer agents targeting microtubules.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1383-1391
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• 2016

Bovine embryonic stem cells have potential for use in research, such as transgenic cattle generation and the study of developmental gene regulation. The Nanog may play a critical role in maintenance of the undifferentiated state of embryonic stem cells in the bovine, as in murine and human. Nevertheless, efforts to study the bovine Nanog for pluripotency-maintaining factors have been insufficient. In this study, in order to understand the mechanisms of transcriptional regulation of the bovine Nanog, the 5'-flanking region of the Nanog was isolated from ear cells of Hanwoo. Results of transient transfection using a luciferase reporter gene under the control of serially deleted 5'-flanking sequences revealed that the -134 to -19 region contained the positive regulatory sequences for the transcription of the bovine Nanog. Results from mutagenesis studies demonstrated that the Sp1-binding site that is located in the proximal promoter region plays an important role in transcriptional activity of the bovine Nanog promoter. The electrophoretic mobility shift assay with the Sp1 specific antibody confirmed the specific binding of Sp1 transcription factor to this site. In addition, significant inhibition of Nanog promoter activity by the Sp1 mutant was observed in murine embryonic stem cells. Furthermore, chromatin-immunoprecipitation assay with the Sp1 specific antibody confirmed the specific binding of Sp1 transcription factor to this site. These results suggest that Sp1 is an essential regulatory factor for bovine Nanog transcriptional activity.

• Korean Journal of Applied Biomechanics
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• v.17 no.2
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• pp.1-9
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• 2007

Walking is not only an essential component of the human mobility, but also is a good exercise. Inability to walk freely can reduce an individual's quality of life and independence substantially. Being a relatively low impact activity, walking is particularly good for the elderly and research has shown that regular walking in the elderly reduces the chance of fall-related injuries and mental diseases as well. In spite of the documented benefits of regular walking, it is still difficult to walk without the aid of assistive devices for the frail elderly who have lower extremity problems. Assistive walking devices(AWD), such as crutches, canes, hiking-poles, T-Poles and walkers, are often prescribed to the elderly to make their walking be safe and efficient. Many researchers have demonstrated the effects of AWDs such as reducing lower extremity loading, improved dynamic/gait stability, yet, no study has been done for gait pattern when the elderly gait with AWDs. Therefore, the purpose of this study was to examine whether T-Poles, one of the AWDs, change the elderly gait pattern. Eight community-dwelling female elderly participated in this study. Laboratory kinematics during walking with T-Poles(PW) and with out T-Poles(NPW) was assessed. PW showed significant increase in step width, stride length, gait velocity and decrease in swing time. No significances were found in lower body joint angles but meaningful trend and pattern were found. Maybe the reason was due to the participants. Our participants were healthy enough so that the effect of T-Poles was minimum. PW also showed typical gait phases which are no single support phase during a gait cycle. It indicates that walking with T-Poles may guarantee safe and confident walking to the frail elderly.

• Journal of Environmental Policy
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• v.10 no.3
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• pp.49-71
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• 2011

Water is an essential element required for the survival of 6 billion human beings around the world, and it has limited mobility. The importance of water industry has grown considerably, as its forecasted market size is expected to increase from $336 billion in 2007 to $865 billion in 2025, respectively. In 2003, the domestic water market was estimated to be worth 11 trillion won. However, according to the estimates of this research, the result above was overestimated by 2 trillion won. The economic ripple effects of newly defined water industry based on the input-output data from 2000 to 2008 were trivial, as the price effects of water industry was considerably minor at only 0.12%. To successfully implement policies to enhance the water industry, the market price mechanism must operate and function properly. Nevertheless, the impact of water's market prices on the economy upon application is projected to be trivial.

• Journal of the Korean Society of Costume
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• v.52 no.5
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• pp.91-107
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• 2002

The purpose of this study was to investigate the experimental design by using deconstructive design and mobile design depicted on Hussein Chalayan' works. Hussein Chalayan, the Turkish-Cypriot who is based in London, says' Challenging is the best word for me'. And so word, 'radical' that has difficult connotations was chosen for this study It implies two menainigs for the word. The first thing is "extreme" - something that is drastic. And 'Applied to clothes', it can probably mean "experimental". He has iconoclastic ideas and his ramp shows are always high on concept, experimentation of art and utility. His approach to fashion derives from philosophical and intellectual theories of deconstruction and mobility, which he expresses through his designs. Deconstructionism, in fashion, rejects customary rules and breaks all conventions. It questions aesthetic norms about bodily proportions and the criteria of beauty, emphasizes the adding on, or discovery of, an irrational moment, and reveals the processes of tailoring in clothing. The shape and the construction of the garment is more important than the color. Cuts. tears, asymmetries, matching different materials are among the most evident features of the deconstructive design. And Chalayan performed the mobile design of transforming furniture into clothes. Chair covers became dresses. a coffee table became a skirt which were designed by Chalayan, with geometric and architectural references. Chalayan says he was inspired by the idea of refugees fleeing. Besides Chalayan uses clothing as an art to reinterpret and reform the human body in a continuous tour de force of body/identity conceptualism and dressmaking. He reflect the body's function in the cultural context of architecture, science, or nature - and then attempt to translate his findings into clothing.dings into clothing.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.5 no.7
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• pp.1329-1345
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• 2011

After the 9.11 terror attack, lawful Interception (LI) has emerged as an important tool for anti-terrorist activity. Law enforcement agents and administrative government bodies effectively monitor suspicious target users of permanent IP-based network devices by LI in Packet Data Networks (PDNs). However, it is difficult to perform LI in monitoring migrating users from a location to another, who change their IPs due to the proliferation of portable Internet devices enabling 3G IP Multimedia Subsystems (IMS). The existing, manual handover technique in 3G IMS makes it even more difficult to continue the LI activities due to time-lag reissuance of LI authority warrants when the target users move to a new LI jurisdiction via a roaming service. Our proposed model is a seamless LI handover mechanism in 3G IMS to support mobility detection of the target users. The LI warrants are transferred to the new LI agent automatically with the target users when they move to a new LI jurisdiction. Thus, time-lag human intervention of reissuance of the LI warrants is removed and enables the LI authorities to continue monitoring. In the simulation of our proposed mechanism, the quality of lawful interception achieves a mean score of over 97.5% out of the possible 100% maximum score, whereas the quality of the existing mechanism has a mean score of 22.725%.

• BMB Reports
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• v.35 no.5
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• pp.524-531
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• 2002

Thrombotic thrombocytopenic purpura (TTP) is characterized by widespread platelet thrombi in arterioles and capillaries. Unusually large or multimeric von Willebrand factor, as well as one or ore platelet-agglutinating factors, have been implicated in the pathogenesis of TTP. But, the actual mechanisms of platelet agglutination have not been satisfactorily explained. Recent studies suggested the 37-kDa platelet-agglutinating protein (PAP) p37 to be partially responsible for the formation of platelet thrombi in patients with TTP. We studied mobility in SDS-PAGE, the sequence of N-terminal amino acid residues, DNA and antigenic characteristics of PAP p37, which might be related to the pathogenesis of TTP. PAP p37 was purified from the plasma of a 31-year-old male Korean patient with acute TTP. The findings are as follows: (1) We compared PAP p37 with thrombin through the use of SDS-PAGE, either with or without $\beta$-mercaptoethanol. PAP p37 did not appear to be cleaved between the A- and B-chains of prethrombin 2. However, thrombin did cleave between those of prethrombin 2, but linked with disulfide bridge. (2) N-terminal 21 amino acid sequence of PAP p37 was T-F-G-S-G-E-A-D-X-G-L-R-P-L-F-E-K-K-S-L-E. It appeared to be identical to that of 285-305 amino acid residues of human prothrombin (prethrombin 2). (3) No prothrombin gene DNA mutation was revealed. (4). The antigenicity of PAP p37 was similar to thrombin, which was a result of the competitive binding against the anti-thrombin antibody. With these results, we conclude that PAP p37 has similar characteristics to prethrombin2.