• 제목/요약/키워드: Human growth hormone

검색결과 192건 처리시간 0.028초

The Efficacy of Enhanced Growth by Ectopic Expression of Ghrelin and Its Variants Using Injectable Myogenic Vectors

  • Xie, Q.F.;Wu, C.X.;Meng, Q.Y.;Li, N.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권1호
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    • pp.146-152
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    • 2004
  • Ghrelin is an acylated peptide recently identified as the endogenous ligand for the growth hormone (GH) secretagogues receptor 1a (GHS-R1a) and is involved in a novel system for regulating GH release. To understand the long-term effects of ghrelin, here we constructed six myogenic expression vectors containing the cDNA of swine mature ghrelin (pGEM-wt-sGhln, pGEM-wt-hGhln), ghrelin mutant of $Ser^3$ with $Trp^3$ (pGEM-mt-sGhln, pGEM-mt-hGhln) and truncated ghrelin derivative (pGEM-tmtsGhln, pGEM-tmt-hGhln) encompassing the first 7 residues of ghrelin (including $Ser^3$ substituted with $Trp^3$) and adding a basic amino acid, Lys (K) in the C-terminus. The constructs, pGEM-wt-sGhln, pGEM-mt-sGhln and pGEM-tmt-sGhln were linked with the ghrelin leader sequence, while the pGEM-wt-hGhln, pGEM-mt-hGhln and pGEM-tmt-hGhln were linked with a leader sequence from the human growth hormone releasing hormone (hGHRH). Intramuscular injection of 200 ${\mu}g$ pGEM-wt-sGhln or pGEM-tmt-sGhln augmented growth over 3 weeks in normal rats and peaked at day 21 or 14 post-injection respectively, whose body weight gains were on average approximately 6% or 19% heavier over controls. However, other injectable vectors had no such enhanced growth effects. Our results suggested that the efficacy of the ghrelin leader sequence was more effective than that of hGHRH in our system. Moreover, the results indicated that skeletal muscle might have the ability to posttranslationally modify the in vivo expressed ghrelin. And the most strikingly, the short ghrelin analog seems to mimic the biological effects more efficiently when compared with the full-length ghrelin.

최근 척수손상 백서에서 인체지방조직유래 중간엽 줄기세포 이식 및 성장호르몬의 투여가 신경회복에 미치는 영향 (The Effect of Human Adipose Tissue Derived Mesenchymal Stem Cells and Growth Hormone on the Recovery of Neurological Deficits due to Experimental Spinal Cord Injury in Rat)

  • 이근철;문인선;허정;권용석;김석권;손희동
    • Archives of Plastic Surgery
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    • 제35권1호
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    • pp.13-19
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    • 2008
  • Purpose: Human adipose tissue-derived mesenchymal stem cells(hATSCs) can be differentiated into multiple mesenchymal lineages, including bone, cartilage, and muscle. And growth hormone play important roles in the normal growth and development of the CNS. In this study, we explored whether the transplanted hATSCs and growth hormones could improve functional recoveries from rats with contusive spinal cord injury. Methods: We divided 30 female rats, which were subjected to a weight driven implant spinal cord injury, into 3 groups with 10 rats each; Group A as a control group, group B with hATSCs transplantation on injured region, and group C with hATSCs transplantation and GH administration for 7 days. Then, we researched their neurologic functional recoveries before and 2, 4, and 8 weeks after transplantation using Basso-Beattie-Bresnahan (BBB) locomotor rating scale. And we checked Y-chromosome positive cells by FISH(Fluorescent in situ hybridization) to identify the survival of transplanted mesenchymal stem cells. Results: After 4 weeks of transplantation, the group B and group C showed significant improvement of neurologic function on BBB locomotor rating scale in comparison with the group A(Group A: $13.1{\pm}0.58$, Group B: $14.6{\pm}0.69$, Group C: $14.9{\pm}0.56$). Moreover, the group C displayed meaningful recovery of neurologic function after 8 weeks in comparison with group B (Group B: $15.7{\pm}0.63$, Group C: $16.5{\pm}1.14$). The group A, the control one, improved for 5 weeks after injury, and had no more recovery. On the other hand, Group B and C showed the improvement of neurologic function continuously for 9 weeks after injury. Conclusion: In this study, we found out that hATSCs transplantation have an effect on neurologic functional recovery of spinal cord injured rat and GH injection seems to bring the synergistic results on this good tendency.

Inhibitory Effect of 4-Aryl 2-Substituted Aniline-thiazole Analogs on Growth of Human Prostate Cancer LNCap Cells

  • Baek, Seung-Hwa;Kim, Nak-Jeong;Kim, Seong-Hwan;Park, Kwang-Hwa;Jeong, Kyung-Chae;Park, Bae-Keun;Kang, Nam-Sook
    • Bulletin of the Korean Chemical Society
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    • 제33권1호
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    • pp.111-114
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    • 2012
  • Androgen receptor (AR) is ligand-inducible nuclear hormone receptor which has been focused on key molecular target in growth and progression of prostate cancer. We synthesized a series of 4-aryl 2-substituted aniline-thiazole analogs and evaluated their anti-cancer activity in AR-dependent human prostate cancer LNCap cells. Among them, the compound 6 inhibited the tumor growth in LNCap-inoculated xenograft model.

A549 폐암세포주의 지방세포 분화에 미치는 티록신 및 당질 코르티코이드 호르몬의 상승 효과 (Synergic Effect on Adipocytic Differentiation by a Combination of Thyroxine andGlucocorticoid in A549 Cells)

  • 김현지;문선하;이성호;전병균
    • 생명과학회지
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    • 제33권9호
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    • pp.693-702
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    • 2023
  • 이 연구는 우리 몸에서 포도당 및 에너지 대사에 관련된 호르몬으로 알려진 티록신 및 당질코르티코이드를 지방분화배양액에 단용 혹은 혼용 첨가하여 A549 폐암세포주가 지방세포로의 분화에 미치는 영향을 조사하였다. 각 지방분화배양액에서 A549세포를 2주 동안 배양한 후, A549세포의 세포 성장률과 말단효소 복원효소를 비교하였을 때, 기본 지방분화배양액이나 PGZ기반 지방분화배양액에서 티록신 및 당질코르티코이드가 단용으로 첨가된 경우보다, 두 호르몬이 혼용으로 첨가되었을 때, 세포의 성장의 유의적으로 억제되는 것을 알 수 있었다. 또한, 세포내 축적된 지방 분자를 염색할 수 있는 Oil Red O 염색과 분화된 지방세포에서 분비되는 여러 아디포카인의 발현을 조사하여 각 지방분화배양액에서 A549 세포의 지방분화능력을 비교하였다. 지방세포로의 분화 능력 역시 티록신 및 당질코르티코이드가 단용으로 첨가된 경우 보다, 두 호르몬이 혼용으로 첨가되었을 때, Oil Red O 염색액으로 염색된 세포내 지방 과립의 수와 크기가 유의적으로 증가하는 것을 알 수 있었고, 아디포카인의 발현 유의적으로 증가하는 것을 알 수 있었다. 이러한 연구 결과를 바탕으로 A549 세포에서 지방세포의 분화를 유도할 때, 포도당 대사 관련 두 호르몬의 혼용 처리가 더욱 더 세포 분화를 촉진한다는 것을 알 수 있었고, 여러 다른 암세포주를 두 호르몬을 혼용하여 첨가한 지방분화배양액에서 처리하여 지방 분화 유도에 의한 세포 성장 억제 효과가 가능할 것으로 판단된다. 그러나, 체내의 다른 세포, 특히 미분화 줄기세포에 미치는 영향에 대한 추가적인 연구가 필요할 것으로 판단된다.

소아의 만성신장질환의 치료 (Treatment of chronic kidney disease in children)

  • 이주훈
    • Clinical and Experimental Pediatrics
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    • 제52권10호
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    • pp.1061-1068
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    • 2009
  • 만성 신질환 환아들의 치료는 신대체 요법과 더불어 적절한 식이 요법, 수분, 전해질 및 산-염기의 관리, 신장 골형성 장애의 예방 및 치료, 빈혈과 고혈압, 성장 지연의 치료 등으로 이루어진다. 식이 요법은 환아들의 성장이 충분히 이루어질 수 있도록 탄수화물, 지방, 단백질 및 열량의 공급이 적절하게 이루어져야 한다. 수분, 전해질 및 산-염기의 상태를 확인하고 적절하게 관리해야 한다. 혈청 칼슘, 인, 부갑상선 호르몬 농도가 사구체 여과율에 따른 적절한 목표치 안에서 유지될 수 있도록 인 결합 제제와 비타민 D 제제를 투여하여 신장 골형성 장애를 예방하여야 한다. 적혈구 생산 촉진제와 철분 제제를 투여하여 혈색소 값을 11-12 g/dl로 유지하여야 한다. 수분 및 염분의 균형과 적절한 항고혈압제의 투여로 고혈압을 조절해야 한다. 사람 재조합 성장 호르몬을 투여하여 최종 성인 키를 호전시키도록 한다.

The Effect of Growth Hormone on mRNA Expression of the GABAB1 Receptor Subunit and GH/IGF Axis Genes in a Mouse Model of Prader-Willi Syndrome

  • Lee, Jin Young;Jin, Dong-Kyu
    • Journal of mucopolysaccharidosis and rare diseases
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    • 제1권2호
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    • pp.54-59
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    • 2015
  • Purpose: Growth hormone (GH) therapy substantially improves several cognitive functions in PWS. However, the molecular mechanisms underlying the beneficial effects of GH on cognition remain unclear in PWS. In this study, we investigated the effects of recombinant human GH on the gene expression of GABAB receptor subunits and GH/insulin-like growth factor (IGF) axis genes in the brain regions of PWS-mimicking mice (Snord116del). Methods: Snord116del mice were injected subcutaneously with 1.0 mg/kg GH or saline, once daily for 7 days. The collected brain tissues were analyzed for mRNA content using quantitative PCR (qPCR) in the cerebellum, hippocampus, and cerebral cortex. Results: GH increased the mRNA expression level of the $GABA_{B1}$ receptor subunit ($GABA_{BR1}$) and IGF-1R in the cerebellum. Furthermore, a significant positive correlation was found between the level of $GABA_{BR1}$ mRNA and the expression of the IGF-1R transcript. GH also induced an increase in the mRNA expression of IGF-2 and IGF-2R in the cerebellum. Conclusion: These data indicate that GH may provide beneficial effects on cognitive function through its influences on the expression of $GABA_{BR1}$ and GH/IGF-1 axis genes in PWS patients.

Corticotropin-Releasing Hormone (CRH)에 의한 인간 위암 세포(MKN-28)의 Migration 증가 (Enhancement of Cell Migration by Corticotropin-Releasing Hormone (CRH) in Human Gastric Cancer Cell Line, MKN-28)

  • 천소영;조대호
    • IMMUNE NETWORK
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    • 제4권4호
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    • pp.244-249
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    • 2004
  • Background: Corticotropin-Releasing Hormone (CRH), an important regulator of stress response, has a potent immunoregulatory effect with the ability to promote the growth of various cancer through CRH receptor type 1 under stress. Although the metastasized cancers through cell migration are more aggressive than the primary cancers, little is known about the effect of CRH on cell migration. Gastric cancer is prone to metastasize to other tissues and it is reported that gastric cancer is response to various stresses such as oxidative stress. Herein, we studied the relationship between CRH and gastric cancer cell migration. Methods: We used gastric cancer cell line, MKN-28 and tested the CRH receptor type 1 expression on MKN-28 by RT-PCR. To examine the change in the ability of migration by CRH in MKN-28, cells were incubated with CRH and then migration ability was measured using a cell migration assay. Results: We confirmed that CRH receptor type 1 was expressed in MKN-28 and HaCaT cells. The migration ability of MKN-28 cells was increased by CRH in a time-, dose- dependent manner. Conclusion: These data suggest that CRH increases migration ability in gastric cancer cell line and that CRH may be a critical regulator in the metastasis of gastric cancer cell.

Active Immunization against Adrenocorticotropic Hormone in Growing-Finishing Barrows: An Initial Trial and Evaluation

  • Lee, C.Y.;Baik, K.H.;Jeong, J.H.;Lee, S.D.;Park, J.K.;Song, Y.M.;Kim, Y.S.;Sohn, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권3호
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    • pp.410-415
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    • 2002
  • Adrenal glucocorticoids, secreted by the stimulus of adrenocorticotropic hormone (ACTH), are catabolic hormones in the pig. The present study was conducted to find whether active immunization against ACTH would suppress cortisol secretion accompanied by an increased growth rate in growing-finishing barrows. ACTH was conjugated to keyhole limpet hemocyanin or human histone using glutaraldehyde or 3-maleimidobenzoic acid N-hydroxysuccinimide, under a 2 (ACTH vs no hapten)${\times}$2 (carrier)${\times}$2 (crosslinker) factorial arrangement of treatments. Cross-bred barrows weighing approximately 25 kg were injected with an ACTHcarrier or carrier only conjugate every 4th wk and slaughtered at approximately 110 kg body weight. Antibodies against ACTH were detected in serum, as determined by $[^{125}I]$ACTH-binding activity, in most animals immunized against the ACTH conjugate, but not in carrier only-injected animals, except for the animals which had received the hapten conjugated to histone via glutaraldehyde. The $[^{125}I]$ACTH-binding activity of serum increased after the second booster injection, but overall ACTH antibody titer was very low. Main effect was not detected not only for the carrier and crosslinker but for the hapten in serum cortisol concentration, ADG, loin muscle area, backfat thickness and longissimus muscle composition including fat and protein. In addition, bound $[^{125}I]$ACTH percentage had no relation to cortisol concentration or to any of the above growth-related variables. Results suggest that ACTH or its conjugates used in the present study were not immunogenically potent enough to affect the glucocorticoid secretion and thus the growth of the immunized pigs.

성장장애(成長障碍)에 관(關)한 문헌적(文獻的) 고찰(考察) (The Literatual Study on the Growth Disorder)

  • 정연희;김윤희;유동열
    • 대한한방소아과학회지
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    • 제13권1호
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    • pp.17-62
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    • 1999
  • Recently lots of books on the process of growing have been published on adolescence and their parents hope to grow tall but yet lacked the correct knowledge. Before helping the growth of body, the best way is to do away with the check factors of the human body. Therefore oriental and occidental medicine study on the inhibition of growth and the result of case study about life therapy, kinesiatrics, including medical therapy are as follows: The habits that help the process of growing are deep sleep, balanced diets, daily physical exercise, good posture. It is essential for people to relieve stress. The physical exercises that promote the process of growing are as follows: to stretch out, daily suitable exercise which stimulates the metabolism of human body to increase levels of growth hormone and excite growth plate, to hasten the process of growing, Oriental medicine causes on the inhibition of growth show many factors: kidney trouble is the inhibited cause, spleen ailment is the acquired ground. The lack of essence of the dual principal of the negative and the positive makes people the inhibition of growth. All these things including fire and dryness have close relations.

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식생활이 정서에 미치는 영향에 대한 고찰 (The observation on eating impact on psychological aspect)

  • 방정균
    • 동의신경정신과학회지
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    • 제16권2호
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    • pp.125-133
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    • 2005
  • Object : This paper is designed to inquire into the influence of eating habits on the emotion of modem people. Method : The methodology is used that searches for materials such as books and treatises on the relationship between eating habits and emotion. Result : Meat used for fast food comes from cows that are fed the feed containing growth hormone and antibiotic. Growth hormone extremely supplements fire or yang within the body while antibiotics undermines the process of yin, leading to an accumulation of damp-heat and inflammation of ministerial fire. Additives contained in instant foods also impinge on the process of yin, leading to poor digestion, damp-heat toxins and ministerial fire. Excessive consumption of meat results in incomplete combustion of fat and hinders the formation of essence. Subsequently, ministerial fire becomes hyperactive as a result of the lack of yin energy. Increased meat consumption in the human diet is coupled with unhealthy eating pattern in which people eat too much for dinner and too quickly. Eating too much disturbs digestion, making it slow and incomplete, leading to excess damp-heat conditions, insufficient amount of vital essence and eventually hyperactive ministerial fire. Milk is considered a catalyst of faster growth, making it suitable for calves. Milk intake rapidly increases the balance of yang and eventually cause hyperactivity of ministerial fire. Conclusion : It is estimated that the eating habits of modern people cause the process of yang, making them feel restless, impatient and aggressive.

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