• 한국의류학회지
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• 제45권2호
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• pp.317-334
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• 2021

This study aims to examine the expressive characteristics and meanings of modern in contemporary fashion, especially focusing on Vogue and The New York Times. First, modern signified originality born out of borderless fusion and compromise. It was also expressed to signify diversity and tolerance not bounded by conventions, TPO, areas, ethnic groups, seasons, or gender. Second, modern meant functionality encompassing the comfort of body and mind. It was used to refer to convenient mobility and activity fit for the lifestyle of busy modern people. Third, lightness and naturalness were used as new meanings of beauty in modern fashion. In the fashion of the twenty-first century, the principal meaning of modern was lightness, which was sought after in everything including materials, modes of wearing, and ways of thinking. Finally, modern fashion was expressed as a democratic tool for social reform and used in the meaning of enlightenment to benefit society as well as oneself. The results of this study indicate that constant changes in trends, lifestyles, and psychology of contemporary society have the potential to give new meaning to the concept and the use of the term modern.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.86-86
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• 2003

Research has been in progress for more than a decade to production of useful proteins by genetic modification in cattle. However, the levels of protein production in transgenic cattle have been reported very low. To enhance protein production in transgenic animal, we tried homologous recombination to donor cells for production of transgenic clone cattle through nuclear transfer procedure. Thus, we constructed the two targeting vectors of human thrombopoietin (TPO) at bovine $\beta$-casein locus using homologous recombination with 13.6 kb and 9.6 kb homology. In two targeting vectors, positive selection was through the neomycin resistance gene and negative selection was by the diphtheria toxin (DT). Gene targeting was attempted in bovine embryonic fibroblasts (bEF) and bovine ear skin fibroblasts (bESF). To determine the most appropriate concentration of neomycin for bEF and bESF, G4l8 resistance was confirmed by culturing the cells in various concentrations of the drug and both of the cells were optimally selected at $900 \mu g/ml$ of neomycin. The transfected bEF and bESF by the targeting vectors were colonized efficiently at the ratio of DNA to transfection reagent such as $4 \mu g$:2 ${mu}ell$ and $1 \mu g$:$2 \mu l$. Comparing number of healthy clones from passage 4 to passage 8, bESF (17%) persist in culture for much longer than bEF (6%). The two gene-targeted bESF clones of 30 random-integrated clones with 9.6 kb homology length were confirmed, however, nothing was out of 72 random integration clones with 13.6 kb homology length, The DT also worked more efficiently in clones transfected with the vector of 9.6 kb homology length. Our data suggests that the choice of donor cell for long culture period should be considered to obtain targeted cell clone, and the gene-targeting frequency and the DT working efficiency are dependent on the length of target homology.

• Korean Chemical Engineering Research
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• 제44권1호
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• pp.73-80
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• 2006

본 연구에서는 제대혈 유래의 조혈줄기세포를 효과적으로 배양하기 위한 선행 연구로서 세포 배양 환경에 따른 조혈줄기세포 증식능의 변화를 관찰하였다. 제대혈의 단핵구 세포에서 분리한 $CD34^+$ 세포를 성장인자 조성-I(이하, coc-I) (EPO, GM-CSF, SCF, IL-3) 및 성장인자 조성-II(이하, coc-II) (TPO, G-CSF, SCF, IL-6, Flt3/Flk-2 ligand)가 포함되어 있는 IMDM(Iscove's modified Dulbecco's medium) 및 무혈청 배지(serum free media, SFM)에서 배양하였으며, 우태아혈청(FBS)의 첨가 영향, 2차원 및 3차원 배양 후 각 조건에서의 세포 증식 및 콜로니 형성능을 비교하였다. 일반적으로 coc-I에서의 세포 증식 및 콜로니 증식이 coc-II에서보다 높았다. 3차원 배양(methocult)에서는 가장 높은 세포 증식($2,258{\pm}456$배)을 나타냈으며, 같은 조성의 2차원 배양(IMDM + coc-I + FBS)에서는 가장 높은 콜로니 증식(BFU-E: $652{\pm}19$, CFU-GM: $520{\pm}58$, CFU-GEMM: $339{\pm}100$배)이 나타났다. 배지를 기준으로 보면, coc-II 조성에 우태아혈청이 포함되지 않은 경우를 제외한 모든 경우에서 세포 증식 및 콜로니 증식이 무혈청 배지에서보다 IMDM에서 높았다. 결론적으로, 모든 배양 조건 중에서 'IMDM + coc-I + FBS' 및 'IMDM + coc-I'에서 가장 좋은 콜로니 증식을 보였으며, 우태아혈청의 첨가 및 2차원 배양 조건이 콜로니 증식에 더 효과적인 것으로 확인되었다. 본 연구 결과는 앞으로 조혈줄기세포의 체외 증식에 필요한 공정개발이나 생물반응기 설계에 유용한 정보를 제공할 수 있을 것으로 사료된다.

• 복식
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• 제65권1호
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• pp.150-163
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• 2015

Due to desire for more variation from male consumers within the men's fashion market in Korea, there has been an ongoing development of departmentalization of fashion styles. The Generation-X man, in particular, is the most active member of our current day society, and will be the principal agent in the senior market from here on forth. Thus, this study categorized their preferred fashion styles, and will seek to analyze the behaviors related to the pursuit of clothing of the Generation-X man. The qualitative investigations of the Q-method as well as the quantitative investigation of the SPSS 12.0 were used in conjunction as the method of study. Additionally, images and types resulting from the preceding first-year study entitled "The Type and Characteristics of the Modern Men's Fashion Images" were utilized as part of the surveys. As a result of the Q-method, which was used to derive the preferred fashion styles of the Generation-X man, they were classified into three categories: the classic suit preferring 'conservative class type', the polished, comfortable style preferring 'modern sophisticated type', and the adventure and variety seeking 'uninhibited individualistic style'. After analyzing the factors which influence clothing preference behaviors of these men, a total of seven causes were drawn from the results, including: conservative indifference to fashion, others conscious pursuit of fashion, the aim to show off brand name products, high involvement in the aspect of design, pursuit of TPO, pursuit of self-image, and conformity to fashion. The elements that revealed differences in the behaviors in pursuing clothing within the preferred fashion style types of the Generation-X man included the conservative indifference to fashion, and others conscious pursuit of fashion, which therefore shows that rather than enjoying the fashion lifestyle, these men possess a more conservative disposition instead.

• 복식
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• 제64권8호
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• pp.1-19
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• 2014

The purpose of this study is to do an in-depth examination of Korean women in their 30's on the characteristics of their online fashion shopping, clothes-wearing, and presentation. In-depth interview and quantitative analysis were conducted as study methods. Results of this in-depth analysis showed that the factor with the most significant influence in their lifestyle was "childbirth." Childbirth was a major factor during fashion shopping and clothes-wearing. Also the results showed that the reason they used online shopping was for convenience, efficiency, rationality, pursuit of information, variety, and hedonism. In particular, women in their 30's had a higher motivation for efficiency and rationality compared to those in their 20's, and of those women, married working women showed the highest preference for fashion soho malls. Meanwhile, full-time homemakers, who pursued rationality, used open markets to search for fashion items based on price. Furthermore, the factors that women in their 30's considered during online shopping were price, design, purpose or situation for wearing the clothing, respectively. Compared to the women in their 20's, they emphasized recommendation, product properties, credibility, economy more than women in their 20's. Factors such as marriage and childbirth were more influential than occupation. Meanwhile, the factors that women in their 30's considered for wearing and presentation were time, place, and occasion(TPO), which all showed high importance in in-depth interview and quantitative analysis. Other factors were 'suitable image to self' and 'covering up body figure.'