• Asian Pacific Journal of Cancer Prevention
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• 제13권3호
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• pp.753-759
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• 2012

Radixin, encoded by a gene on chromosome 11, plays important roles in cell motility, invasion and tumor progression. However, its function in pancreatic cancer remains elusive. In this study, radixin gene expression was suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method. We found that radixin shRNA caused down-regulation of radixin in PANC-1 cells, associated with inhibition of pancreatic cancer cell proliferation, survival, adhesion and invasive potential in vitro. When radixin-silenced cells were implanted in nude mice, tumor growth and microvessel density were significantly inhibited as compared to blank control cells or nonsense shRNA control cells. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin-silenced PANC-1 cells. Our results suggest that radixin might play a critical role in pancreatic cancer progression, possibly through invvolvement of down-regulation of TSP-1 and E-cadherin expression.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.937-943
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• 2014

Polymorphisms in miRNA binding sites have been shown to affect miRNA binding to target genes, resulting in differential mRNA and protein expression and susceptibility to common diseases. Our purpose was to predict SNPs (single nucleotide polymorphisms) within miRNA binding sites of inflammatory genes in relation to gastric cancer. A complete list of SNPs in the 3'UTR regions of all inflammatory genes associated with gastric cancer was obtained from Pubmed. miRNA target prediction databases (MirSNP, Targetscan Human 6.2, PolymiRTS 3.0, miRNASNP 2.0, and Patrocles) were used to predict miRNA target sites. There were 99 SNPs with MAF>0.05 within the miRNA binding sites of 41 genes among 72 inflammation-related genes associated with gastric cancer. NF-${\kappa}B$ and JAK-STAT are the two most important signaling pathways. 47 SNPs of 25 genes with 95 miRNAs were predicted. CCL2 and IL1F5 were found to be the shared target genes of hsa-miRNA-624-3p. Bioinformatic methods could identify a set of SNPs within miRNA binding sites of inflammatory genes, and provide data and direction for subsequent functional verification research.

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5667-5672
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• 2014

Folate receptor alpha ($FR{\alpha}$) mediates folate uptake by endocytosis, and while folate is essential to DNA methylation and synthesis and may have an important role in proliferating cells. $FR{\alpha}$ is known to be expressed in rapidly proliferating cells, including many cancer cell lines, but there has been no systematic assessment of expression in cervical cancer cell lines. The aim of the present study was to evaluate the effects of $FR{\alpha}$ on proliferation and apoptosis of cervical cells and correlation mechanism. In this study, we investigated the biological function of $FR{\alpha}$ in Hela cells using RNA interference. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK8) assay, while cell cycling and apoptosis were assessed by flow cytometry, mRNA levels by real time-PCR and protein levels of $FR{\alpha}$, c-Fos and c-Jun by Western blotting. The results revealed that $FR{\alpha}$ was highly expressed in Hela cells and its silencing with a small interfering RNA (siRNA) inhibited cell proliferation and induced cell apoptosis, arresting the cell cycle in G0/G1 stages while decreasing the proportion in S and G2/M stages, and suppressed the expression levels of c-Fos and c-Jun. In conclusion, the results of this study indicated that $FR{\alpha}$ down-regulation might be capable of suppressing cervical cancer cell proliferation and promoting apoptosis. It suggested that $FR{\alpha}$ might be a novel therapeutic target for cervical cancer.

• Journal of Power Electronics
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• 제16권2호
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• pp.685-694
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• 2016

This paper presents a reliability assessment model for the power semiconductors used in wind turbine power converters. In this study, the thermal loadings at different timescales of wind speed are considered. First, in order to address the influence of long-term thermal cycling caused by variations in wind speed, the power converter operation state is partitioned into different phases in terms of average wind speed and wind turbulence. Therefore, the contributions can be considered separately. Then, in regards to the reliability assessment caused by short-term thermal cycling, the wind profile is converted to a wind speed distribution, and the contribution of different wind speeds to the final failure rate is accumulated. Finally, the reliability of an actual power converter semiconductor for a 2.5 MW wind turbine is assessed, and the failure rates induced by different timescale thermal behavior patterns are compared. The effects of various parameters such as cut-in, rated, cut-out wind speed on the failure rate of power devices are also analyzed based on the proposed model.

• Parasites, Hosts and Diseases
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• 제35권1호
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• pp.19-24
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• 1997

The infection status of anisakid larvae was examined in 290 marine fish of 25 species and in 108 cephalopods of 3 species purchased in Bayuquan region, Yingko city nearby the coast of the Bohai Sea from may to August 1992. A total of 7,327 laivae were collected from 156 fish of 19 species and 8 squids of one species. The 3rd-stage larvae of Anisokis simplex were collected from 121 fish (63..4%) of 15 species (N : 191) and from 8 squids (14.8%) of one species (N : %4), and they were total, 5,992 (81.8%). Out of remaining 1,335 larvae, 154 (2.1%) were classified as Rvnnqscnris type B from 23 fish of 4 species, 1,013 (13.8%) as avnnosccris type C from 79 fish of 13 species, 164 (2.2%) as Hvsterothylacium China type V from 20 fish of 4 species, 3 (0.04%) as Raphinoscnris from 3 fish of 2 species and one was Pseunotewonovc necipiens larva. Key words: Bohai Sea, Anisokinae, larvae , Anisckis, HvsterothWLacium, Thwnnascaris, Raphinqscaris , Pseudoterranova.

• 한국통신학회논문지
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• 제39C권1호
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• pp.47-59
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• 2014

본 논문은 실내 환경에서의 엔터테인먼트 활용을 목적으로 쿼드로터형 비행체를 위한 비전 기반의 궤적 추종제어 시스템을 다룬다. 항공촬영 및 감시 등의 특수임무를 완수하기 위해 자율성이 강조되는 실외 비행체와 비교할 때, 엔터테인먼트를 목적으로 하는 실내 환경에서의 비행체를 위해서는 안정성 및 정밀성이 특히 고려된 호버링 및 궤적추종 기능 등이 요구된다. 이에, 본 논문은 동작생성, 자세추정, 궤적추종 모듈로 구성된 궤적추종 제어시스템을 제안한다. 동작생성 모듈은 매 시간에서의 3차원 자세로 기술되는 동작들에 대한 연속적인 시퀀스를 생성한다. 자세추정 모듈은 비행체에 장착된 원형 링 패턴의 인식을 통해 쿼드로터의 3차원 자세정보를 추정한다. 궤적추종 모듈은 동작생성 모듈과 자세추정 모듈로부터 제공되는 정보를 이용하여 쿼드로터 비행체의 3차원 위치를 실시간적으로 제어한다. 제안된 시스템의 성능은 단일 점 추종, 다점 추종, 곡선궤적 추종에 대한 실험을 통해 평가된다.

• 원예과학기술지
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• 제34권1호
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• pp.122-133
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• 2016

While ion beams are widely used in plant breeding, little is known about the sensitivity of Lavandula angustifolia (lavender) to ionizing radiation. To compare the biological effects of different types of ionizing radiation on the germination and survival rates of lavender, we exposed lavender seeds to gamma rays, 3 MeV electron beams, and 1.89 MeV proton ion beams. We observed that the seed germination rate decreased with increasing dosages of all three types of ionizing radiation. The malformation rate of lavender seedlings exposed to electron beams and gamma rays increased with increasing radiation dosage. By contrast, the effect of the accelerated proton beams on the malformation rate was negatively correlated with the dosage used. The survival rate of lavender seedlings exposed to the three types of ionizing radiation decreased in a dose-dependent manner. In addition, the survival rate of seedlings irradiated with proton and electron beams decreased more slowly than did that of seedlings irradiated with gamma rays. The half-lethal dose of gamma rays, electron beams, and proton beams was determined to be 48.1 Gy, 134.3 Gy, and 277.8 Gy, respectively, and the most suitable proton-ion energy for lavender seeds in terms of penetration depth was determined to be 5 MeV. These findings provide valuable information for the breeding of lavender by radiation mutation.

• Parasites, Hosts and Diseases
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• 제57권5호
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• pp.543-547
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• 2019

Toxoplasma gondii can infect humans worldwide, causing serious diseases in pregnant women and immunocompromised individuals. T. gondii rhoptry protein 13 (ROP13) is known as one of the key proteins involved in host cell invasion. In this study, we generated virus-like particles (VLPs) vaccine expressing T. gondii rhoptry ROP13 and investigated VLPs vaccine efficacy in mice. Mice immunized with ROP13 VLPs vaccine elicited significantly higher levels of T. gondii-specific IgG, IgG1, IgG2a, and IgA antibody responses following boost immunization and challenge infection, whereas antibody inductions were insignificant upon prime immunization. Differing immunization routes resulted in differing antibody induction, as intranasal immunization (IN) induced greater antibody responses than intramuscular immunization (IM) after boost and challenge infection. IN immunization induced significantly higher levels of IgG and IgA antibody responses from feces, antibody-secreting cells (ASCs), $CD4^+$ T, $CD8^+$ T cells and germinal center B cell responses in the spleen compared to IM immunization. Compared to IM immunization, IN immunization resulted in significantly reduced cyst counts in the brain as well as lesser body weight loss, which contributed to better protection. All of the mice immunized through either route survived, whereas all na?ve control mice perished. These results indicate that the ROP13 VLPs vaccine could be a potential vaccine candidate against T. gondii infection.

• 동양고전연구
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• 제36호
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• pp.105-134
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• 2009

미술계에서는 오래 전부터 고전 중의 "논어'를 필독서로 여겨왔다. 여기에 나오는 '회사후소(繪事後素)'라는 말은 그림을 그리는 화가는 물론이고 그림을 논하는 사람들까지 모르는 이가 없다. 그러나 그 의미를 "그림 그리는 일은 바탕을 갖춘 뒤에 하는 것이다" 또는 "그림 그리는 일은 나중에 흰색으로 완성한다"라고 해석하여 의견이 분분하다. 그림을 그리는 방법은 시간이 흘러도 잘 변하지 않는다. 학자들은 대개 전문적인 회화를 상투적으로 알고 있으며, 회화적인 기법에서의 흰색의 특수성을 잘 이해하지 못한다. 어떤 경우라도 그림을 그리기 전에 바탕처리를 하는 것이 기본이지만, 그림을 완성할 때 흰색으로 마무리하는 것은 일반적이지 않다. 고대의 채색화라도 오늘날의 비단 그림과 다르지 않다. 삶의 바탕이 되는 소양과 예술적 감각을 키워준 '회사후소'는 화가들의 뇌리에 새겨진 매우 유효한 미학사상이 되었다. '회사후소'에 대한 해석은 예논(禮論)의 시대적인 변화와 관련되지만, 본고에서는 순수하게 미술학적 입장에서 고찰하였다.

• Korean Journal of Acupuncture
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• 제38권4호
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• pp.209-221
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• 2021

Objectives : The aim of this study was to compare and analyze studies related to fire acupuncture (FA) published in South and North Korea. Methods : FA studies published in South Korea were searched through domestic and overseas databases (KISS, RISS, OASIS, and EMBASE). For North Korean studies, databases from the Information Center on North Korea and Korea Institute of Science and Technology Information were used. Among the searched literatures, clinical studies using FA as a treatment intervention were selected (i.e., randomized controlled trials, and case reports). Finally, information such as the disease for which FA was used, the characteristics of FA, and details of treatment methods for FA were extracted from the screened literatures and the results of South and North Korean studies were compared and analyzed. Results : This review included 28 South Korean studies and 9 North Korean studies. All studies in South Korea, except for the three articles that used traditional FA treatment, applied "warm needling"-like acupuncture (WA) in the form of heating the handle of the needle after inserting the needle into the skin, and most studies were conducted on diseases of the musculoskeletal system. Whereas in the North Korean studies, FA was applied to various diseases such as trigeminal neuralgia, skin diseases, and gastrointestinal diseases, and standardized FA tools were used. Conclusions : Studies on traditional FA treatment have rarely been reported in South Korea. The amount of information described in the FA studies in North Korea was limited. In this review, the characteristics of FA treatment tools in South and North Korean studies were mainly analyzed. In the future, FA studies from more diverse perspectives are needed.