• Journal of Microbiology and Biotechnology
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• v.21 no.7
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• pp.697-702
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• 2011

A strain of the cyanobacterium Arthrospira was isolated from Lake Chahannaoer in northern China and was characterized according to microscopic morphology, photosynthetic oxygen-evolving activity, growth rate, and nutritional profile. Compared with thermophilic Arthrospira species occurring naturally in tropical and subtropical lakes, this isolate is mesophilic and grows optimally at ${\sim}20^{\circ}C$. The total protein, fatty acid, phycocyanin, carotenoid, and chlorophyll a contents were 67.6, 6.1, 4.32, 0.29, and 0.76 grams per 100 grams of dry weight, respectively. The strain is rich in polyunsaturated fatty acids (PUFAs). An essential omega-3 fatty acid, docosahexaenoic acid (DHA), was detected, and ${\gamma}$-linolenic acid (GLA) and DHA accounted for 28.3% of the total fatty acid content. These features of this newly isolated strain make it potentially useful in commercial mass culture in local areas or as a biofuel feedstock. It is also an alternative resource for studying the metabolic PUFA pathways and mechanisms of cold stress tolerance in cyanobacteria.

• Korean Journal of Pharmacognosy
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• v.28 no.4
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• pp.179-184
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• 1997

To search for useful antioxidants from plant materials, we investigated the antioxidative activity in the methanol extracts of 30 Chinese medicinal plants using DPPH method. The highest activity $(RC_{50}:\;12\;{\mu}g)$ was showed in the methanol extract of Rosa rugosa, followed by Potentilla fruticosa $(14\;{\mu}g)$, P. fragarioides $(16\;{\mu}g)$. And Geum aleppicum $(18\;{\mu}g)$. From the leaves of R. rugosa, two antioxidative compounds were isolated by a bioassay guided purification and identified as isoquercitrin and ${\beta}-glucogallin$ on the basis of $^1H-,\;^{13}C-\;NMR$, and FAB-MS data. ${\beta}-Glucogallin$ is the first report in this plant. The DPPH radical scavenging activity of ${\beta}-glucogallin\;(RC_{50}:\;8.5\;{\mu}g)$ was more effective than those of ${\alpha}-tocopherol\;(12\;{\mu}g)$ and BHA $(14\;{\mu}g)$.

• Genes and Genomics
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• v.40 no.11
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• pp.1119-1125
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• 2018

Melanocortin 4 receptor: (MC4R) and Myostatin (MSTN) are two important growth trait-related genes in animals. In this study, we showed that two SNPs, MC4R-719A>G and MSTN-519C>T, found in the promoters of the MC4R and MSTN genes, respectively, are both associated with growth traits in Spinibarbus hollandi. Furthermore, we observed that there were significant associations between the expression levels of the MC4R and MSTN genes and these two growth trait-related SNPs. The expression level of MC4R gene in brain was lower in GG genotype fish with extremely high growth performance than that in AA genotype fish with extremely low growth performance. Expression level of the MSTN gene in muscle was lower in TT genotype fish with extremely high growth performance than that in CC and CT genotype fish with lower growth performance. The results indicated that these SNPs located in the promoters of MC4R and MSTN are associated with growth-related traits through modification of gene expression levels. The MSTN and MC4R SNPs may have useful application in effective marker-assisted selection aimed to increase output in S. hollandi.

• Journal of Drive and Control
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• v.18 no.3
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• pp.8-15
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• 2021

A rear axle steering (RAS) system is attached to the rear of medium and large commercial vehicles that transport large cargo. The existing RAS systems are driven by electro-hydraulic actuator (EHA), and most commercialized EHAs consist of electric motors, hydraulic pumps, relief valves, prefill valves and cylinders. The prefill valve required for such EHAs is a type of check valve with extremely low cracking pressure that should not allow RAS to have noise or vibration, and the prefill valve prevents system negative pressure as well as unstable operation. Most papers on this topic rely on experiments to predict valve performance, and theoretically detailed modeling of valves or pipelines is performed, but it is very rare to evaluate hydraulic vibration characteristics by analysing everything from hydraulic pumps to valves comprehensively. In this study, we proposed an experimental circuit that can predict the performance of the prefill valve. The study also analysed the pressure-flow pulsation that is transmitted to the valve through the pipeline, and how the transmitted pressure-flow pulsation affects the valve vibration.

• Structural Engineering and Mechanics
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• v.78 no.5
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• pp.623-635
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• 2021

Previous studies have suggested the maximum experimental story shear force of beam-column joint frame does not reach its theoretical value due to beam-column joint failure when the column-to-beam moment capacity ratio was close to 1.0. It was also pointed out that under a certain amount of axial force, an axial collapse and a sudden decrease of lateral load-carrying capacity may occur at the joint. Although increasing joint transverse reinforcement could improve the lateral load-carrying capacity and axial load-carrying capacity of beam-column joint frame, the conditions considering varying axial force were still not well investigated. For this purpose, 7 full-scale specimens with no-axial force and 14 half-scale specimens with varying axial force are designed and subjected to static loading tests. Comparing the experimental results of the two types of specimens, it has indicated that introducing the varying axial force leads to a reduction of the required joint transverse reinforcement ratio which can avoid the beam-column joint failure. For specimens with varying axial force, to prevent beam-column joint failure and axial collapse, the lower limit of joint transverse reinforcement ratio is acquired when given a column-to-beam moment capacity ratio.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.173-182
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• 2023

Cancer is a global public health issue that becomes the second primary cause of death globally. Considering the side effects of radio- or chemo-therapy, natural phytochemicals are promising alternatives for therapeutic interventions to alleviate the side effects and complications. Ginsenoside Rh2 (GRh2) is the main phytochemical extracted from Panax ginseng C.A. Meyer with anticancer activity. GRh2 could induce apoptosis and autophagy of cancer cells and inhibit proliferation, metastasis, invasion, and angiogenesis in vitro and in vivo. In addition, GRh2 could be used as an adjuvant to chemotherapeutics to enhance the anticancer effect and reverse the adverse effects. Here we summarized the understanding of the molecular mechanisms underlying the anticancer effects of GRh2 and proposed future directions to promote the development and application of GRh2.

• International Journal of High-Rise Buildings
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• v.11 no.4
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• pp.265-276
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• 2022

Double-Skin Facades (DSF) on tall buildings are becoming increasingly common in urban environments due to their ability to provide architectural merit, passive design, acoustic control and even improved structural efficiency. This study aims to understand the effects of porous DSF on the aerodynamic characteristics of tall buildings using wind tunnel tests. High Frequency Force Balance and pressure tests were performed on the CAARC standard tall building model with a variable porous DSF on the windward face. The introduction of a porous DSF did not adversely affect the overall mean forces and moments experienced by the building, with few differences compared to the standard tall building model. There was also minimal variation between the results for the three porosities tested: 50%, 65% and 80%. The presence of a full-height porous DSF was shown to effectively reduce the mean and fluctuating wind pressure on the side face of the building by about 10%, and a porous DSF over the lower half height of the building was almost as effective. This indicates that the porous DSF could be used to reduce the design load on cladding and fixtures on the side faces of tall buildings, where most damage to facades typically occurs.

• Journal of Microbiology and Biotechnology
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• v.33 no.12
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• pp.1681-1691
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• 2023

Flavin mononucleotide-binding proteins or domains emit cyan-green fluorescence under aerobic and anaerobic conditions, but relatively low fluorescence and less thermostability limit their application as reporters. In this work, we incorporated the codon-optimized fluorescent protein from Chlamydomonas reinhardtii with two different linkers independently into the redox-responsive split intein construct, overexpressed the precursors in hyperoxic Escherichia coli SHuffle T7 strain, and cyclized the target proteins in vitro in the presence of the reducing agent. Compared with the purified linear protein, the cyclic protein with the short linker displayed enhanced fluorescence. In contrast, cyclized protein with incorporation of the long linker including the myc-tag and human rhinovirus 3C protease cleavable sequence emitted slightly increased fluorescence compared with the protein linearized with the protease cleavage. The cyclic protein with the short linker also exhibited increased thermal stability and exopeptidase resistance. Moreover, induction of the target proteins in an oxygen-deficient culture rendered fluorescent E. coli BL21 (DE3) cells brighter than those overexpressing the linear construct. Thus, the cyclic reporter can hopefully be used in certain thermophilic anaerobes.

• Journal of the Korean Society of Visualization
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• v.22 no.2
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• pp.55-62
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• 2024

Using an internal flow noise test bench, this study investigates the variation in internal flow noise at the inlet and outlet monitoring points of a DN100 T-junction pipe under different flow velocities. Results indicate that with increasing flow velocity, both the sound pressure level and total sound pressure level at the inlet and outlet monitoring points increase. The highest total sound pressure level is observed at the vertical outlet monitoring point B, followed by the horizontal inlet monitoring point A, with the lowest at the horizontal outlet monitoring point C. At a constant flow velocity, the sound pressure level at the inlet and outlet points initially increases and then decreases as frequency increases.

• Journal of Microbiology and Biotechnology
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• v.27 no.12
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• pp.2119-2128
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• 2017

Citrinin (CIT) is a toxic secondary metabolite produced by fungi belonging to the Penicillium, Aspergillus, and Monascus spp. This toxin has been detected in many agricultural products. In this study, a strain Y3 with the ability to eliminate CIT was screened and identified as Cryptococcus podzolicus, based on the sequence analysis of the internal transcribed spacer region. Neither uptake of CIT by cells nor adsorption by cell wall was involved in CIT elimination by Cryptococcus podzolicus Y3. The extracellular metabolites of Cryptococcus podzolicus Y3 stimulated by CIT or not showed no degradation for CIT. It indicated that CIT elimination was attributed to the degradation of intracellular enzyme(s). The degradation of CIT by C. podzolicus Y3 was dependent on the type of media, yeast concentration, temperature, pH, and initial concentration of CIT. Most of the CIT was degraded by C. podzolicus Y3 in NYDB medium at 42 h but not in PDB medium. The degradation rate of CIT was the highest (94%) when the concentration of C. podzolicus Y3 was $1{\times}10^8cells/ml$. The quantity of CIT degradation was highest at $28^{\circ}C$, and there was no degradation observed at 3$5^{\circ}C$. The study also showed that acidic condition (pH 4.0) was the most favorable for CIT degradation by C. podzolicus Y3. The degradation rate of CIT increased to 98% as the concentration of CIT was increased to $20{\mu}g/ml$. The toxicity of CIT degradation product(s) toward HEK293 was much lower than that of CIT.