• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2001년도 봄 학술발표회 논문집
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• pp.43-48
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• 2001

The use of fly ash in cement and concrete industries has many benefits including engineering, economic, and ecological aspects. However, it has a disadvantage of low strength development, especially at early ages. In this study, in order to overcome this problem, the early strength accelerating agent($NA_{2}$ $SO_{4}$) was selected and applied to the production of high strength concrete(HSC) containing fly ash. It was found that the compressive strength of fly ash concrete incorporating TEX>$NA_{2}$ $SO_{4}$ has greater than that of concrete containing fly ash only until 7 days after casting. From the microstructural point of view, ettringite increased and pores decreased in fly ash concrete incorporating TEX>$NA_{2}$ $SO_{4}$ , leading to the development of early age strength. It was also found that the velocity vs. strength relationship of HSC is considerably different from that of low-strength concrete(LSC). Therefore, in order to predict early age strength of HSC, a estimation equation different from that for LSC is needed.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2006년도 춘계학술발표회 논문집(I)
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• pp.434-437
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• 2006

Structures tend to become high-rise, large and specialized due to the urban concentration. Technology related to the structure and construction is required to improve, for which the use of high strength concrete(HSC) with better material property, and composite member with the combined advantage of both concrete and steel for better performance, is suggested. Over a half of fires, which increase by over 10% every year recently, come from the architectural structure, causing a loss at national level. However, little study has been conducted on the member at high temperature despite the increase in the use of HSC composite members. In this study, the techniques of modeling for analysing by DIANA (Displacement Analyzer) the fire damaged HSC composite compressive members are researched. We can review the effect of change in the steel ratio, section size and the steel ratio on the residual strength of structural members by parameter analysis study.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2010년도 춘계 학술대회 제22권1호
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• pp.441-442
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• 2010

본 연구는 최근 국내 초고층 건물이나 초장대 교량에 쓰이는 고강도 콘크리트의 강도 발현을 임피던스 계측을 통해 발현된 강도를 모니터링 하는 시스템을 개발하고 무선 센서를 이용하여 현장에서 실시간으로 측정할 수 있는 시스템을 개발하는데 그 목적을 두었다.

• Smart Structures and Systems
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• 제23권5호
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• pp.495-506
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• 2019

This paper presents the results of axial pressure testing on reinforced concrete columns (RCCs) filled with confined normal concrete (NC) and high-strength concrete (HSC) using glass-fiber reinforced plastic pipes (GRP) casing as well as fiber reinforced polymer (FRP). This study aims to evaluate the behavior and mechanical properties of columns confined with GRP casing and FRP wrapping under pressure loads. The major parameters in the experiments were the type of concrete, the effect of GRP casing and FRP wrapping, as well as the number of FRP layers. 12 cylindrical RCCs (150*600) mm were prepared and divided into two groups, NC and HSC, and each group was divided into two parts. In each part, one column was without FRP strengthening layer, a column was wrapped with one FRP layer and another column with two FRP layers. All columns were tested under concentrated compression load. The results of the study showed that the utilization of FRP wrapping and GRP casing improved compression capacity and ductility of RCCs. The addition of one and two layers-FRP wrapping increased compression capacity in the NC group to an average of 18.5% and 26.5% and to an average of 10.2% and 24.8% in the HSC group. Meanwhile, the utilization of GRP casing increased the compression capacity of the columns by 4 times in the NC group and 3.38 times in the HSC group. The results indicated that although both FRP wrapping and GRP casing result in confinement, the GRP casing resulted in increased compression capacity and ductility of the RCCs due to higher confinement. Furthermore, the confinement effect was higher on columns made with NC.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.393-396
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• 2008

본 논문에서는 띠철근 간격에 따른 고강도 콘크리트 기둥의 내화 성능에 대한 실험결과를 제시하고 있다. 단면 크기 305${\times}$305mm이 고강도 콘크리트 기둥을 3개 제작하였으며, 띠철근 간격은 150, 210, 300mm 이었다. 시험시의 압축강도는 69MPa이었다. 시험 결과로서, 고강도 콘크리트 기둥의 내화 성능은 띠철근 간격에 큰 영향을 받는 것으로 나타났다. 띠철근 간격이 줄어들수록 내화 성능이 증가하는 것으로 나타났다.

• Steel and Composite Structures
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• 제11권6호
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• pp.455-468
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• 2011

Although retrofitting and strengthening reinforced concrete (RC) columns by wrapping fiber reinforced polymer (FRP) composites have become a popular technique in civil engineering, the study on reinforced high-strength concrete (HSC) columns is still not sufficient. The objective of these companion papers is to investigate the mechanical properties of reinforced HSC square columns confined by aramid FRP (AFRP) jackets under concentric compressive loading. In the part I of these companion papers, an experiment was conducted on 54 confined RC specimens and nine unconfined plain specimens, the considered parameters were the concrete strength, the thickness of AFRP jackets, and the form of AFRP wrapping. The experimental process and results are presented in detail. Subsequently, some discussions on the confinement effect, failure modes, strength, and ductility of the columns are carried out.

• Computers and Concrete
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• 제7권3호
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• pp.221-237
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• 2010

In this study 72 different high strength concrete (HSC) mixes were produced according to the Taguchi design of experiment method. The specimens were divided into four groups based on the range of their compressive strengths 40-60, 60-80, 80-100 and 100-125 MPa. Each group included 18 different concrete mixes. The slump and air-content values of each mix were measured at the production time. The compressive strength, splitting tensile strength and water absorption properties were obtained at 28 days. Using this data the Genetic Programming technique was used to construct models to predict mechanical properties of HSC based on its constituients. These models, together with the cost data, were then used with a Genetic Algorithm to obtain an HSC mix that has minimum cost and at the same time meets all the strength and workability requirements. The paper describes details of the experimental results, model development, and optimization results.

• Biomolecules & Therapeutics
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• 제15권4호
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• pp.261-265
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• 2007

Hydroxyproline (HYP) is a post-translational product of proline hydroxylation catalyzed by an enzyme prolyl 4-hydroxylase (P4H) which plays a crucial role in the synthesis of all collagens. Considering the role of collagen and its significance in many clinically important diseases such as liver fibrosis, a great deal of attention has been directed toward the development of an assay at cell-based system. The reason is that cell-based assay system is more efficient than enzyme-based in vitro system and takes much less time than in vivo system. Several assay procedures developed for P4H are laborious, time-consuming and not feasible for the massive-screening. Here, we report the cell-based assay method of prolyl 4-hydroxylase in immortalized rat hepatic stellate HSC-T6 cells. To optimize the cell culture condition to assay for HYP content, various concentrations of reagents were treated for different times in HSC-T6 cells. Our data showed that the treatment with ascorbate in a hypoxic condition for 24 h resulted in the maximal increase of HYP by 1.8 fold. Alternatively, cobalt chloride ($5\;{\mu}M$) and ascorbate ($50\;{\mu}M$) in normoxic states exhibited similar effect on the production of HYP as in a hypoxic condition. Therefore, cobalt chloride can be substituted for a hypoxic condition when an anaerobic chamber is not available. Rosiglitazone and HOE077, known as inhibitors of collagen, synthesis decreased P4H enzyme activity by 32.3% and 15%, respectively, which coincided with previous reports from liver tissues. The level of the smooth muscle ${\alpha}$-actin, a marker of activated stellate cells, was significantly increased under hypoxia, suggesting that our experimental condition could work for screening the anti-fibrotic compounds. The assay procedure took only 3 days after treatment with agents, while assays from the primary stellate cells or liver tissues have taken several weeks. Considering the time and expenses, this assay method could be useful to screen the compounds for the inhibitor of prolyl 4-hydroxylase.

• International Journal of Oral Biology
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• 제41권1호
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• pp.1-8
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• 2016

OSCC is currently the most common malignancy of the head and neck, affecting tens of thousands of patients per year worldwide. Natural flavonoids from plants are potential sources for novel anti-cancer drugs. Icariin is the active ingredient of flavonol glycoside, which is derived from the medical plant Herba Epimedii. A metabolite of icariin, icariside II exhibits a variety of pharmacological actions, including anti-rheumatic, anti-depressant, cardiovascular protective, and immunomodulatory functions. However, the exact mechanism causing the apoptosis-inducing effect of icariside II in OSCC is still not fully understood. In the present study, we assessed the anti-cancer effect of icariside II in OSCC cell lines by measuring its effect on cell viability, cell proliferation, and mitochondria membrane potential (MMP). Icariside II treatment of OSCC cells resulted in a dose- and time-dependent decrease in cell viability. Hoechst staining indicated apoptosis in icariside II-treated HSC cells. Icariside II inhibited cell proliferation and induced apoptosis in HSC cells, with significant increases in all present parameters in HSC-4 cells. The results clearly suggested that icariside II induced apoptosis via activation of intrinsic pathways and caspase cascades in HSC-4 cell lines. The collective findings of the study suggested that Icariside II is a potential treatment for OSCC; in addition, the data could provide a basis for the development of a novel anti-cancer strategy.

• 한국양식학회지
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• 제12권2호
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• pp.91-100
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• 1999

The complete nucleotide sequence of olive flounder (Paralichthys olivaceus) hsp70-related rDNA was determined by RT- and RACE-PCR methods. A full-length of hsp70-related cDNA has an open reading frame of 1.95 kb encoding 650 amino acids with a calculated molecular weight of 71.1 kD. A corresponding hsp70-related protein contains a number of conserved elements including an ATP-binding domain, a nuclear localization signal and the carboxyl terminal motif, EEVD, which may have a role in chaperone function. Comparison of nucleotide and predicted amino acid sequence between olive flounder hsp70-related gene and hsp/hsc70 genes of other species revealed a high similarity with the cognate form of these genes. These results indicated that we recovered likely to be a olive flounder cognate hsc70 gene.