• Nutrition Research and Practice
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• v.5 no.4
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• pp.281-287
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• 2011

In this study, we evaluated the antioxidant activity of pine needle extracts prepared with hot water, ethanol, hexane, hot water-hexane (HWH), and hot water-ethanol (HWE), using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical method. The hot water extract possessed superior antioxidant activity than the other extracts. We also compared the antioxidant activity of pine needle extracts through ROS inhibition activity in a cellular system using MC3T3 E-1 cells. The hot water extract exhibited the lowest ROS production. The pattern of HPLC analysis of each extract indicated that the hot water extract contained the highest proanthocyanidin level. The pine needle hot-water extract was then isolated and fractionated with Sephadex LH-20 column chromatography to determine the major contributor to its antioxidant activity. The No.7 and 12 fractions had high antioxidant activities, that is, the highest contents of proanthocyanidins and catechins, respectively. These results indicate that the antioxidant activity of procyanidins from the hot water extract of pine needles is positively related to not only polymeric proanthocyanidins but also to monomeric catechins. Moreover, the antioxidant activity of the pine needle hot water extract was similar to well-known antioxidants, such as vitamin C. This suggests that pine needle proanthocyanidins and catechins might be of interest for use as alternative antioxidants.

• Biomedical Science Letters
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• v.18 no.1
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• pp.35-41
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• 2012

Physiological activities of hot water extract and solvent fractions isolated from Astragalus membranaceus were examined and the antioxidative, fibrinolytic, thrombin inhibitory and a-glucosidase inhibitory activity were measured. The hot water extract of Astragalus membranaceus was fractionated into hexane, chloroform, ethyl acetate, butanol and water fractions, and each of these fractions was individually assayed. The antioxidative activities of ethyl acetate and chloroform fractions were 89.96% and 87.36%, respectively. Using the fibrin plate method, only the hot water extract showed a plasmin activity of 0.41 units/ml. The thrombin inhibitory activity of the ethyl acetate fraction was the highest with a value of 82.73%. The hot water extract displayed a-glucosidase inhibitory activity of 64.91%. In conclusion, the hot water extract and the ethyl acetate fraction can be used as materials for the development of biofunctional foods to prevent cardiovascular diseases.

• The Korean Journal of Food And Nutrition
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• v.20 no.3
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• pp.289-294
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• 2007

For the purpose of developing a functional yogurt, a new type of yogurt was prepared. To manufacture this new yogurt, 12% skim milk was added to the hot water extract of black soybeans. The yogurt was then evaluated for its acid production(pH and titratable acidity), number of viable cells, viscosity, quality-retention properties, and sensory properties. The titratable acidity of the yogurt with the added 5% black soybean hot-water extract was higher(2.07%) than that of the yogurt without the added extract(1.80%). The viscosity of the yogurt made by adding 5% black soybean hot-water extract was higher(5,040 cps) than that of the yogurt made with only skim milk(2,740 cps). Lactic acid bacteria propagation was stimulated by the addition of the 5% black soybean hot-water extract. When the yogurt prepared with the black soybean hot-water extract was kept at $7^{\circ}C$ for 14 days, its quality-retention was comparatively good. Finally the overall sensory score of the yogurt made with the added 5% black soybean hot-water extract was slightly lower than that of the yogurt made with only skim milk.

• Journal of Haehwa Medicine
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• v.23 no.1
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• pp.105-114
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• 2014

Objectives : In this study, the antioxidant activities of the 80% ethanol and hot water extracts of Euphorbia supina Rafinesque were investigated. Methods : We measured total phenol contents, flavonoid contents, 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The production of reactive oxygen species was measured in LPS-induced RAW 264.7 cells using flow cytometry system. Results : The content of phenol in the hot water extract was $65.529{\pm}0.462mg/g$ and $126.932{\pm}1.894mg/g$ in the 80% ethanol extract, and that of flavonoid in the hot water extract was $16.063{\pm}0.471mg/g$ and $29.159{\pm}1.963mg/g$ in the ethanol extract. The 80% ethanol extract also showed higher DPPH and ABTS radical scavenging activities ($90.8{\pm}1.0%$ and $92.5{\pm}0.7%$) than the hot water extract ($81.5{\pm}0.5%$ and $91.5{\pm}0.2%$). The production of reactive oxygen species(ROS) was reduced dose-dependently by 80% ethanol and hot water extract at concentration of 1, 10 and $100{\mu}g/m{\ell}$ of RAW 264.7 cells. Conclusion : According to these results, the 80% ethanol extract of Euphorbia supina Rafinesque has a good anti-oxidative effects than the hot water extract. Thus, the 80% ethanol extract of Euphorbia supina Rafinesque may serve as useful natural antioxidants.

• The Korean Journal of Community Living Science
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• v.14 no.2
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• pp.41-47
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• 2003

To investigate the effects of hot water soluble extract from Angelicae Gigantis Radix on lipid metabolism and antioxidant defense system in rats fed hypercholesterol diet, Sprague-Dawley male rats were fed either hypercholesterol diet with water or hot water soluble extract from Angelicae Gigantis Radix. Hypercholesterol diet contained 18 % beeftallow and 5 % cholesterol. After 4 weeks of experimental periods serum and liver obtained. The level of serum lipid and the activities of GOT and GPT was measured. The level of lipid and TBARS and the activity of GSH-Px was measured also. The results obtained were: 1) Food intake in the experimental group supplied hot water soluble extract from Angelicae Gigantis Radix was low significantly. 2) In the experimental group supplied hot water soluble extract from Angelicae Gigantis Radix of serum total lipid and total cholesterol was significantly low. 3) Serum GOT activity was significantly low in the experimental group supplied hotwater soluble extract from Angelicae Gigantis Radix. There was no difference of serum GPT activity between control and experimental group supplied hot water soluble extract from angelical Gigantis Radix. 4) There was no difference of lipid composition, TBARS level and GSH-px activity in liver between control and experimental group. The result of this study indicates that the hot water soluble extract from Angelicae Gigantis Radix was effective in decreasing the food intake of the experimental animals in the hypercholesterol diet and also effective in decreasing the levels of serum total lipid, total cholesterol and GOT activity. However it was not effective to change the level of lipid, TBARS and GSH-Px activity of liver.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1200-1210
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• 2006

This experiment was designed to investigate the effect of the CMT and MCMT hot water extract & ultra-fine powder on microglia and memory deficit model. The effects of the CMT and MCMT hot water extract on expression of IL-l${\beta}$, IL-6, TNF-${\alpha}$, NOS-II, COX-2, IL-10, TGF-${\beta}$1 mRNA and production of IL-lP, IL-6, TNF-a, NO, ROS in BV2 microglial cell line treated by lipopolysacchaide(LPS) ; serum glucose, uric acid, AChE activity of the memory deficit mice induced by scopolamine , behavior of the memory deficit mice induced by scopolamine and were investigated, respectively. The CMT and MCMT hot water extract suppressed the expression of IL-1${\beta}$, IL-6, TNF-${\alpha}$, NOS-II, COX-2 mRNA, production of IL-l${\beta}$, IL-6, TNF-${\alpha}$, NO, ROS and increased the expression of IL-10, TGF-${\beta}$l mRNA in BV2 microglial cell line treated by LPS. The MCMT hot water extract & ultra-fine powder increased glucose, decreased uric acid and AChE significantly in the serum of the memory deficit mice induced by scopolamine. The CMT and MCMT hotwater extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. According to the above result, it is suggested that the CMT and MCMT hot water extract & ultra-fine powder might be usefully applied for prevention and treatment of dementia.

• The Journal of Korean Medicine
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• v.28 no.2 s.70
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• pp.137-154
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• 2007

Objective : This experiment was designed to investigate the effect of the GYZB hot water extract & ultra-fine powder on the Alzheimer's disease model induced by amyloid ${\beta}$ protein (${\beta}A$). Method : We measured the effects of the GYZB hot water extract on expression of $IL-1{\beta}$, IL-6 mRNA and production of IL-6, $TNF-{\alpha}$ in the BV2 microglial cell line treated with lipopolysaccharide (LPS). The effects of the GYZB hot water extract & ultra-fine powder on (1) the behavior, (2) expression of $IL-1{\beta}$ and $TNF-{\alpha}$, (3) glucose in serum, (4) the infarction area of the hippocampus, and brain tissue injury in mice induced with Alzheimer's diseased by ${\beta}A$ were investigated. Results : The GYZB hot water extract suppressed the expression of $IL-1{\beta}$ and IL-6 mRNA and significantly suppressed the production of IL-6 and $TNF-{\alpha}$ in the BV2 microglial cell line treated with LPS. The GYZB hot water extract & ultra-fine powder showed a significant inhibitory effect on the memory deficit of the mice with Alzheimer's disease induced by ${\beta}A$ in the Morris water maze experiment, which measured stop-through latency and distance movement-through latency. The GYZB ultra-fine powder significantly suppressed the expression of $IL-1{\beta}$ and $TNF-{\alpha}$ protein, and the GYZB hot water extract significantly suppressed the expression of $TNF-{\alpha}$ protein in the microglial cell of mice with Alzheimer's disease induced by ${\beta}A$. The GYZB hot water extract & ultra-fine powder reduced the infarction area of hippocampus in the mice with Alzheimer's disease induced by ${\beta}A$. Conclusions : These results suggest that GYZB hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of GYZB for Alzheimer's disease is suggested for future research.

• Journal of the Korean Society of Food Culture
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• v.21 no.5
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• pp.550-558
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• 2006

We developed a kind of soy-based sauce for stews using three recipes and sensory evaluation processes, and examined the biological activities such as antioxidative, fibrinolytic and ${\alpha}$-glucosidase inhibitory activities of hot water extract of Cornus officinalis S. et Z in order to use as functional ingredient. The developed stew was composed of soy sauce $1\frac{1}{2}$ Tbsp, sugar $1\frac{1}{4}$ Tbsp, garlic, small green onion, powdered sesame and sesame seed oil $\frac{1}{2}$ tsp, respectively, ginger extract $\frac{1}{4}$ tsp, black pepper $\frac{1}{8}$ and water $\frac{2}{3}$C per 300 g of main ingredients(pork rib, chicken and dried pollack). It was evaluated more highly acceptable than the original recipes by sensory test. Replacement of water in the recipe of the developed stew sauce with hot water extract of Cornus officinalis S. et Z did' nt affect the sensory quality. From the above results. we concluded that hot water extract of Cornus officinalis S. et Z might be a excellent source to provide health functionality.

• 보존과학연구
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• s.20
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• pp.21-47
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• 1999

According to damage of papers by the chemical cause, papers become to acidify. Among the 3 kinds of method measuring the acidity of paper, one was nondestructive method measuring the surface of the paper by flat type electrode and the others were destructive methods measuring directly by cold water extract method and hot water extract method. In case of the cellulolytic cultural properties, the latter methods were not applied because those methods must bedissociated papers. To measure the paper acidity nondestructively, we investigated the correlation of the nondestructive method and the destructive methods. The conclusions are as follows. 1) In the relationship of the cold water extract method and the hot water extract method, It was indicated that the pH measured by the hot water extract method was high quality printing paper 0.08, rough printing paper 0.13, and Korean paper 0.29 higher than that by the cold water extract method.2) In the relationship of the cold water extract method and the surface measurement method, It showed that the pH value measured by the cold water extract method was high quality printing paper 1.86, rough printing paper 0.80,and Korean paper 0.58 higher than value that by the surface measurement.3) In the relationship of the hot water extract method and the surface measurement method, It showed that the pH value measured by the hot ABSTRACT water extract method was high quality printing paper 1.78, rough printing paper 0.66, and Korean paper 0.29 higher than that by the surface measurement. From the above-mentioned results, the pH value measured by the surface measurement was need to high about 1.78∼1.86 in high quality printing paper, 0.66-0.80 in rough printing paper, and 0.29∼0.58 in Korean paper and the surface measurement with flat type electrode was very available to measure the acidity of Korean papers actually.

• 보존과학연구
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• s.20
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• pp.49-64
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• 1999

According to damage of papers by the chemical cause, papers become to acidify. Among the 3 kinds of method measuring the acidity of paper, one was nondestructive method measuring the surface of the paper by flat type electrode and the others were destructive methods measuring directly by cold water extract method and hot water extract method. In case of the cellulolytic cultural properties, the latter methods were not applied because those methods must bedissociated papers. To measure the paper acidity nondestructively, we investigated the correlation of the nondestructive method and the destructive methods. The conclusions are as follows. 1) In the relationship of the cold water extract method and the hot water extract method, It was indicated that the pH measured by the hot water extract method was high quality printing paper 0.08, rough printing paper 0.13, and Korean paper 0.29 higher than that by the cold water extract method.2) In the relationship of the cold water extract method and the surface measurement method, It showed that the pH value measured by the cold water extract method was high quality printing paper 1.86, rough printing paper 0.80,and Korean paper 0.58 higher than value that by the surface measurement.3) In the relationship of the hot water extract method and the surface measurement method, It showed that the pH value measured by the hot ABSTRACT water extract method was high quality printing paper 1.78, rough printing paper 0.66, and Korean paper 0.29 higher than that by the surface measurement. From the above-mentioned results, the pH value measured by the surface measurement was need to high about 1.78∼1.86 in high quality printing paper, 0.66-0.80 in rough printing paper, and 0.29∼0.58 in Korean paper and the surface measurement with flat type electrode was very available to measure the acidity of Korean papers actually.