• Title/Summary/Keyword: Hot cell

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Cytoprotective effect of polysaccharide isolated from different mushrooms against 7-ketocholesterol induced damage in mouse liver cell line (BNL CL. 2)

  • Kim, Joo-Shin;Chung, Hau Yin;Na, Keun
    • Nutrition Research and Practice
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    • v.1 no.3
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    • pp.180-183
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    • 2007
  • Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris- were prepared by hot water extraction and alcohol precipitation. Cytoprotective ability was evaluated by measuring the viable cells of the normal embryonic liver cell line (BNL CL. 2) in the presence of 7-ketocholesterol. At $80\;{\mu}g/mL$ of 7-ketocholesterol, cytotoxicity was very high with a loss of 98% of viable cells after 20 h of incubation. With the addition of $200\;{\mu}g/mL$ of each polysaccharide isolate to the cell line containing $80\;{\mu}g/mL$ of 7-ketocholesterol, polysaccharide isolates from both Flammulina velutipes and Peurotus ostreatus could significantly inhibit the 7-ketochoelsterol-induced cytotoxicity in the cells. But other polysaccharide isolates were not effective in inhibiting cell damage caused by the oxLDL-induced cytotoxicity.

Electrode fabrication and Performance of Single Cell with an area of $2000\;cm^2$ in PAFC ($2000\;cm^2$ 대면적 인산형 연료전지 전극 제조 및 단위전지 성능 특성)

  • Song, Rak-Hyun;Shin, Dong-Ryul;Kim, Chang-Soo;Lee, Byung-Rok;Choi, Soo-Hyun
    • Proceedings of the KIEE Conference
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    • 1997.07d
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    • pp.1308-1310
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    • 1997
  • Several methods for electrode fabrication of phosphoric acid fuel cell(PAFC) have been studied. The conventional methods that include a coating method and a rolling method, have disadvantages of a very complicated drying process and a hot pressing process for making a large electrode. In this study, to solve these problems, the mixing method of coating and rolling processes has been developed. In the mixing method, the electrode was coated on the electrode support and, after drying the coated layer, was rolled at room temperature and then sintered at $350^{\circ}C$. The single cell performances of the electrodes fabricated by several methods were examined and the mixing method appeared a good cell performance of 0.65 V, $260\;mA/cm^2$. Also the single cell with an area of $2000\;cm^2$ was manufactured and its performance attained 0.593 V, 300 A.

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Cytotoxicity of Artemisia argyi Extract Against H9 (ATCC HTB 176) Cell and Antioxidant Enzyme Activities (황해쑥(Artemisia argyi)의 H9 (ATCC HTB176) 세포에 대한 세포독성 및 항산화효소 활성)

  • 김경하;정대영;민태진;박시원
    • YAKHAK HOEJI
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    • v.43 no.5
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    • pp.598-605
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    • 1999
  • The hot water and mathanol extracts of Artemisia argyi showed considerable cytotoxicities against H9(ATCC HTB 176) cancer cell with IC50 values of $48.6{\;}\mu\textrm{g}/ml$ and $51.9{\;}\mu\textrm{g}/ml$, respectively. These cytotoxicities were found to be dependent on the extract concentrations and culture days. CuZnSOD and MnSOD activities were significantly increased in the cytoplasm and mitochondria fractions of cancer cell, and media in the presence of Artemisia argyi. Such enhanced SOD activities were generally in the range of two to threefolds. In contrast to SOD, catalase and glutathione peroxidase activities were not detected at all. These results suggest that Artemisia argyi have generated $O_2^-$ in the mitochondria and cytoplasm of H9 cancer cell with concurrent induction of CuZnSOD and MnSOD in situ, which dismutate $O_2^-{\}to{\;}H_2O_2$. Without coordinated actions of catalase and/or glutathione peroxidase $H_2O_2$ is easily converted to very toxic OH and these reactive oxygen species together might have induced necrosis and/or apoptosis of H9 cell.

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A Channel Borrowing Scheme using Genetic Algorithm in Cellular Mobile Computing Environment (셀룰라 이동 컴퓨팅 환경에서 유전 알고리즘을 이용한 채널차용 기법)

  • Lee, Seong-Hoon;Lee, Dong-Woo;Lee, Sang-Gu
    • Journal of KIISE:Information Networking
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    • v.29 no.2
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    • pp.165-173
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    • 2002
  • In the static channel assignment scheme for cellular mobile computing environment, each cell has a fixed number of channels and supports a service for a user′s request entering to the cell. This scheme has an advantage of simplicity. However, this scheme has a disadvantage that can′t control far hot cell problem. Therefore, to solve this problem, the "channel borrowing" concept is needed. In this paper, we propose a load balancing(channel borrowing, channel reassignment) approach using genetic algorithm. The purposes of using genetic algorithm in this paper are ${\circled1}$ to find early a cell including an available channel and ${\circled2}$ to decrease a number of probings and ${\circled3}$ to migrate to the cell after searching an available channel near upon optimality. To represent effectiveness of the proposed algorithm, we simulated various experiments.

Survival of Food-borne and Pathogenic Microorganisms in Hot Spring Water (온천수에서 식중독 및 병원성 미생물의 생존 양상)

  • Zheng Jian-Bin;Ahn Yong-Sun;Jeong Do-Yeong;Kim Yong-Suk;Shin Dong-Hwa
    • Journal of Food Hygiene and Safety
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    • v.21 no.1
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    • pp.36-40
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    • 2006
  • This study was conducted to investigate the effects of hot spring water against the survival of food-borne and pathogenic microorganisms. Staphylococcus aureus, Bacillus cereus, and Escherichia coli, which are food-borne microorganisms, Candida albicans and Trichophyton mentagrophytes, which are skin disease pathogens, and Helicobacter pylori, gastritis inducing microorganism, were tested. The content of fluoride in tested hot spring water is 14.1 mg/L, which is higher than the standard of safe for drinking water 1.5 mg/L, but the results on 48 other items were up to the standard. Hot spring water didn't show the bactericidal effects against food-borne microorganisms, C. albicans, and H. pylori tested. However, the viable cell populations of B. cereus and T. mentagrophytes were decreased, which were depends on the temperature of hot spring water. From these results, we confirmed that hot spring water didn't show the bactericidal effects against food-borne microorganisms, skin disease pathogens, and gastritis inducing microorganism, but the growth of some microorganisms were inhibited by high temperature ($41^{\circ}C$).

The Characteristics of Kimchi by the Degree of Hotness of Powdered Red Pepper (매운 정도가 다른 고춧가루로 제조한 김치 품질 특성)

  • Jeong Eun-Ja;Bang Byung-Ho;Kim Kwan-Pil
    • The Korean Journal of Food And Nutrition
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    • v.18 no.1
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    • pp.88-93
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    • 2005
  • The objective of this study was to investigate an influence of the degree of hotness of powdered red pepper on quality attributes of Kimchi. The extent of hotness was analysed by content of capsaicin and dihydrocapsaicin and ASTA color value, and the change of quality attributes of samples were evaluated by pH, acidity, total microbes, lactic acid bacteria cell count and sensory. Content of capsaicin and dihydrocapsaicin in mild red pepper powder was 11.4mg%, 5.4mg% respectively. And content of capsaicin and dihydrocapsaicin in hot red pepper powder was 149.1mg%, 66.0mg% respectively. Kimchi was made with mild and hot red pepper and stored for 24 days at 5±1℃. The pH of Kimchi made with mild red pepper powder during storage of 12 days was slightly lower than that of Kimchi with hot red pepper powder. The pattern of change in pH of samples showed a reducing trend during storage, and the acidity was vice-versa. At day 0, numbers of total bacteria in mild and hot Kimchi were 5.7, 6.7 log cfu/g, respectively. And at day 3, number of total bacteria in mild and hot Kimchi was same. The change in number of lactic acid bacteria was similar to that of total bacteria. In case of sensory evaluation, Kimchi made with hot red pepper powder showed better sensory scores in overall acceptability.

Dehydration of Opuntia ficus-indica and Aloe vera Slices Using Polyethylene Glycol and Comparison with Other Drying Methods (폴리에틸렌글리콜 처리에 의한 백년초, 알로에 절편의 탈수 및 건조와 기존 건조 방법과의 비교)

  • Yu, Dong-Jin;Wang, Suk-Mae;Song, Kyung-Bin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.7
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    • pp.1024-1029
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    • 2010
  • Opuntia ficus-indica and Aloe vera slices were dried using 20, 30, and 40% polyethylene glycol (PEG) 4,000 as a dehydration agent, and the dried samples were compared with the hot-air dried and freeze dried in terms of rehydration ratio, color, and sensory evaluation. The moisture content of the PEG-treated samples decreased with increasing concentrations of polyethylene glycol. The rehydration ratio of the PEG-treated samples was better than those of the hot air-dried or freeze-dried samples. The color of the PEG-treated samples was similar to that of the freeze-dried samples and better than that of the hot air-dried samples. The sensory evaluation of PEG-treated samples was better than those of the hot air-dried or freeze-dried samples. These results suggest that dehydration of Opuntia ficus-indica and Aloe vera slices using PEG is very effective in terms of rehydration ratio and minimal damage of cell structure.

Quality Characteristics of Osmotic Dehydrated Sweet Pumpkin by Different Drying Methods (건조방법에 따른 삼투처리 단호박의 품질 특성)

  • Hong, Joo-Heon;Lee, Won-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.9
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    • pp.1573-1579
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    • 2004
  • This study was conducted to develop intermediate material for new processed food from sweet pumpkin. Osmotic dehydration was carried out as pretreatment before drying. After the sweet pumpkins were pretreated under optimized osmotic dehydration conditions, they were dried by three drying methods (hot air drying, vacuum drying and cold air drying). The moisture contents of dried sweet pumpkin products by osmosis treatment (sucrose) and hot air drying, vacuum drying, and cold air drying were 14.48, 14.09 and 13.87%, respectively. Cold air drying preserved more vitamin C content and showed lower color difference than hot air drying and vacuum drying. As a result of microscopic analysis, cold air dried sweet pumpkin was observed regular tissue, while hot air and vacuum dried sweet pumpkins were observed a cell collapse following the loss of water.

Fabrication of high-quality silicon wafers by hot water oxidation (Hot water oxidation 공정을 이용한 고품위 실리콘 기판 제작)

  • Park, Hyo-Min;Tark, Sung-Ju;Kang, Min-Gu;Park, Sung-Eun;Kim, Dong-Whan
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.06a
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    • pp.89-89
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    • 2009
  • 높은 소수반송자 수명(life-time)을 가지는 고품위 실리콘 기판은 고효율 실리콘 이종접합 태양전지 제작을 위한 중요 요소 기술 중 하나이다. 본 연구에서는 n-type c-Si 기판을 이용한 고효율 실리콘 이종접합 태양전지제작을 위해 hot water oxidation(HWO) 공정을 이용하여 고품위 실리콘 기판을 제작하였다. 실리콘 기판의 특성 분석은 Qusi-steady state photoconductance (QSSPC)를 이용하여 소수반송자 수명을 측정하였으며, 기판의 면저항 및 wetting angle을 측정하여 공정에 따른 특성변화를 분석하였다. Saw damage etching 된 기판을 웨이퍼 표면으로부터 particle, 금속 불순물, 유기물 등의 오염을 제거하기 위해 $60{\sim}85^{\circ}C$로 가열된 Ammonia수, 과산화수소수($NH_4OH/H_2O_2/H_2O$), 염산 과산화수소수($HCL/H_2O_2/H_2O$) 및 실온 희석불산(DHF) 중에 기판을 각각 10분 정도씩 침적하여, 각각의 약액 처리 후에 매회 10분 정도씩 순수(DI water)에서 rinse하여 RCA 세정을 진행한 후 HWO 공정을 통해 기판 표면에 얇은 산화막 을 형성시켜 패시베이션 해주었다. HF를 이용하여 자연산화막을 제거시 HWO 공정을 거친 기판은 매끄러운 표면과 패시베이션 영향으로 기판의 소수 반송자 수명이 증가하며, 태양전지 제작시 접촉저항을 감소시켜 효율을 증가 시킬수 있다. HWO 공정은 반응조 안의 DI water 온도와 반응 시간에 따라 life-time을 측정하여 진행하였으며, 이후 PE-CVD법으로 증착된 a-Si:H layer 및 투명전도 산화막, 금속전극을 증착하여 실리콘 이종접합 태양전지를 제작하였다.

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A Study on The Comparison of The Program Efficiency in The Conventional CHE Injection Method and a novel Hot Electron Injection Method Using A Substrate forward Bias (CHE 주입방법과 기판 순바이어스를 이용한 새로운 고온 전자 주입방법의 프로그램 효율성 비교에 관한 연구)

  • Zhang, Yong-Jie;An, Ho-Myoung;Kim, Hee-Dong;Kim, T.G.
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.47 no.1
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    • pp.1-5
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    • 2010
  • In this paper, we directly compare the program efficiency of conventional channel hot electron (CHE) injection methods and a novel hot electron injection methods using substrate forward biases in our silicon-oxide-nitride-oxide-silicon (SONOS) cell. Compared with conventional CHE injection methods, the proposed injection method showed improved program efficiency including faster program operation at lower bias voltages as well as localized trapping features for multi-bit operation with a threshold voltage difference of 1 V at between the forward and reverse read. This program method is expected to be useful and widely applied for future nano-scale multi-bit SONOS memories.