• Journal of Life Science
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• v.12 no.4
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• pp.477-482
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• 2002

As a biological model system for the production of an active antibacterial peptide, we have attempted the expression and secretion of insect defensin in Saccharomyces cerevisiae. Nucleotide sequences encoding mature defensin composed of 40 amino acids were fused in frame with promoter and signal sequence of Saccharomyces diastaticus glucoamylase, and mating factor $\alpha$ l[MF $\alpha$1] prosequence. The host strain, S. cerevisiae 2805 was transformed with the resulting plasmid, pSMFll The secretion of functional defensin was confirmed by growth inhibition zone assay using Micrococcus luteus as a test organism. Insect defensin was secreted to the culture supernatant in biologically active form by glucoamylase signal sequence and mating factor $\alpha$1 prosequence. Most of antibacterial activity was detected in the culture supernatant. Defensin was also active against Staphylococcus aureus and Listeria monocytogenes.

• International Journal of Industrial Entomology and Biomaterials
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• v.13 no.1
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• pp.37-43
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• 2006

Enterococcus faecalis was isolated from the body fluid of dead Galleria mellonella larvae. Upon injection of E. faecalis into the hemocoel of G. mellonella, the bacteria destroyed parts of humoral defense systems in the hemolymph. In a test for the proteolytic activity of E. faecalis CS, it was confirmed that the enzyme degraded three well-known a-helical antimicrobial peptides, cecropin A, melittin and halocidin, and abolished their activities. We also determined putative cleavage sites on the primary sequences of three peptides through purification and mass analysis of peptide fragments digested by E. faecalis CS. Furthermore it was found that apolipophorin-III, recently known as a critical recognition protein for invading microbes in the hemolymph of G. mellonella, was also degraded by E. faecalis CS. Taken together, the present work shows that the protease in secretions from E. faecalis destroyed two critical humoral immune factors in the hemolymph of G. mellonella larvae. In addition, this paper demonstrates that the relationship between the host insect and the pathogenic bacteria might provide a valuable model system to study the enterococcal virulence mechanism, which may be relevant to mammalian pathogenesis.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.1
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• pp.89-95
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• 2011

In this study, We modeled the deck house of the container ship like the representative bluff body and made the model ship. By using the PIV technique, the exhaust gas anti-reflux effect of the deck house backward according to open and close of the Sunken Deck and installation of the deflector in deck house side were measured in circulating water channel. The experiment system consists of hi-speed camera, laser, image board, host computer. The mean velocity vector and time mean axial velocity were found in deck house backward and the results were compared each case.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.23-23
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• 2018

Taiwan, formerly known as Formosa, located on tropical and subtropical climate zones with abundant biological resources. According to the latest version of the Flora of Taiwan, there are 4339 species of vascular plants including 1054 endemic species. First, Taiwania (Taiwania cryptomerioides), named after its native island of Taiwan, have been isolated more than 500 secondary metabolites, including lignans, terpenoids, steroids, and flavonoids. Several of the metabolites are reported to have antibacterial, antifungal, antimite, antitermite and antitumor activities. In order to investigate plant secondary metabolic diversity toward industrial applications, we established deep transcriptome resources for non-model plants and fungi to produce terpenoid metabolites of economic importance. Second, many plants of Lauraceae have been utilized in folk medicine for their exciting bioactivities. The twigs and leaves from 27 tree species of Lauraceae grown in Taiwan were performed to evaluate potential bioactivity. The leaves of Cinnamomum osmophloeum are traditionally used in folk medicines, and many biological activities have been identified, such as antibacterial, antifungal, antitermite, antidiabetic, antihyperuricemia, antiinflammatory, and antioxidant activities. However, C. osmophloeum has nine chemotypes with various secondary metabolite profiles. In order to efficiently produce active compounds, we established the genetic markers to identify the chemotype plants. Finally, Cinnamomum kanehirae is the host of the medicinal mushroom Antrodia cinnamomea. Several in vivo and in vitro studies indicated that A. cinnamomea possesses a diverse range of biological activities. Because of the potential pharmacological application, we established the transformation system to enhance the triterpenoid contents production.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.2
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• pp.31.2-31.2
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• 2010

We have performed a spectroscopic study of globular cluster (GC) system associated with the Virgo cD galaxy M87 using the Subaru/FOCAS MOS mode. We derive ages, metallicities and abundance ratios from the GC spectra using Simple Stellar Population (SSP) models. The metallicity distribution function (MDF) obtained empirically based on Milky Way GCs is consistent with the MDF derived from SSP models. A comparison with a meta-analysis using literature data sample of 15 other GC systems shows good agreement with our results. The properties of GCs acquired from the spectra will be used to test the recent theoretical prediction of a significant inflection along the colour-metallicity relations (Yoon et al. 2006). If confirmed, the non-linearity of the relations would shed new light on the interpretation of the GC colour bimodality. The robustness of our results is being tested against the choice of a SSP model, measurement errors and sample selection towards the goal of better understanding the formation history of GCs and host galaxy.

• BMB Reports
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• v.41 no.2
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• pp.102-107
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• 2008

Extracellular proteases play an important role in a wide range of host physiological events, such as food digestion, extracellular matrix degradation, coagulation and immunity. Among the large extracellular protease family, serine proteases that contain a "paper clip"-like domain and are therefore referred to as CLIP-domain serine protease (clip-SP), have been found to be involved in unique biological processes, such as immunity and development. Despite the increasing amount of biochemical information available regarding the structure and function of clip-SPs, their in vivo physiological significance is not well known due to a lack of genetic studies. Recently, Drosophila has been shown to be a powerful genetic model system for the dissection of biological functions of the clip-SPs at the organism level. Here, the current knowledge regarding Drosophila clip-SPs has been summarized and future research directions to evaluate the role that clip-SPs play in Drosophila immunity are discussed.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.30-33
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• 2003

The chestnut blight fungus, Cryphonectria parasitica, and its hypovirus aye a useful model system in the study of the mechanisms of hypoviral infection and its consequences, such as a biological control of fungal pathogens. Strains containing the double-stranded (ds) RNA viruses Cryphonectria hypovirus 1 show characteristic symptoms of hypovirulence and display hypovirulence-associated changes, such as reduced pigmentation, sporulation, laccase production, and oxalate accumulation. Interestingly, symptoms caused by hypoviral infection appear to be the result of aberrant expression of a number of specific genes in the hypovirulent strain. Several viral regulated fungal genes are identified as cutinase gene, Lac1, which encodes an extracellular laccase, Crp, which encodes an abundant tissue-specific cell-surface hydrophobin that mediates physical strength, and Mf2/1 and Mf2/2, which encode pheromone genes involved in poor sporulation in the presence of hypo-virus. Since the phenotypic changes in the fungal host are pleiotropic, although coordinated and specific, it has been suggested that the hypovirus disturbs one or several regulatory pathways (Nuss,1996). Accordingly, several studies have shown the implementation of a signal transduction pathway during viral symptom development. Although further studies are required, hypovirulence and its associated symptom development due to the hypoviral regulation of a fungal hetero-trimeric G-protein have been suggested. In addition, recent studies have shown the presence of a novel protein kinase gene cppk1 and its transcriptional upregulation by hypovirus. In this review, the presence of important components in signal transduction pathway, their putative biological function, and viral-specific regulation will be addressed.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1997.04a
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• pp.413-417
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• 1997

Distributed piezoelectric sensor and actuator have been designed for efficient vibration control of a cantilevered beam. Both PZT and PVDF are used in this study, the former as an actuator and the latter as a sensor for our integrated structure. For the PZT actuator, the position and size have been optimized. Optimal electrode shape of the PVDF sensor has been determined. For multi-mode vibration control, we have used two PZT actuators and a PVDF sensor. Electrode shading of PVDF is more powerful for modal force adjustment than the sizing and positioning of PZT. Finite element method is used to model the structure that includes the PZT actuator and the PVDF sensor. By deciding on or off of each PZT segment, the length and the location of the PZT actuator are optimize. Considering both of the host structure and the optimized actuators, it is designed that the active electrode width of PVDF sensor along the span of the beam. Actuator design is based on the criterion of minimizing the system energy in the control modes under a given initial condition. Sensor is designed to minimize the observation spill-over. Modal control forces for the residual(uncontrolled) modes have been minimized during the sensor design. Genetic algorithm, which is suitable for this kind of discrete problems, has been utilized for optimization. Discrete LQG control law has been applied to the integrated structure for real time vibration control. Performance of the sensor, the actuator, and the integrated smart structure has been demonstrated by experiments.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.10
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• pp.4042-4059
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• 2020

The development of new multimedia techniques such as 3D printing is increasingly attracting the public's attention towards 3D objects. An optimized robust and imperceptible watermarking method based on Ant Colony Optimization (ACO) and Weber Law is proposed for 3D polygonal models. The proposed approach partitions the host model into smaller sub meshes and generates a secret watermark from the sub meshes using Weber Law. ACO based optimized strength factor is identified for embedding the watermark. The secret watermark is embedded and extracted on the wavelet domain. The proposed scheme is robust against geometric and photometric attacks that overcomes the synchronization problem and authenticates the secret watermark from the distorted models. The primary characteristic of the proposed system is the flexibility achieved in data embedding capacity due to the optimized strength factor. Extensive simulation results shows enhanced performance of the recommended framework and robustness towards the most common attacks like geometric transformations, noise, cropping, mesh smoothening, and the combination of such attacks.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.35 no.12
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• pp.75-82
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• 2019

After 1840 the first opium war, that changed the social nature and living habitats in China. The purpose of this study is to observe the influence of the war on the plan layout of traditional Chinese residential houses in Beijing Siheyuan. To find out the changes that were made after 1840, and reservation of the past, the space syntax model is used to analyze different social contexts and also compare the ancient and modern residential buildings. The result of the study indicate the following: Before the war, the ancient Siheyuan people's way of life strictly abide by the hierarchy system; External emphasis shows the host's status. However, in modern times, the social status became equal, efficient and harmonious relationship internally, while externally, it arranged the orientation and position of buildings to adapt to the natural environment. However, the boundaries between family members and visitors the emphasis on the courtyard and respect for private life remain unchanged.