• Title/Summary/Keyword: Host range

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Identification, Pathogenicity and Host Range of a Potential Bioherbicide, Epicoccosorus nematosporus, Causing Fingerprint Stem Blight on Water Chestnut, Eleokaris kuroguwai (생물제초제로서의 올방개 지문무늬병균(Epicoccosorus nematosporus)의 동정, 병원성 및 기주범위)

  • 홍연규;조재민;김재철;엄재열
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.58-65
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    • 1996
  • 한국의 수도 재배 지대에서 문제가 되는 다년생 논잡초 올방개에 자연상태에서 기주특이적으로 기생하여 올방개 줄기를 고사시키는 병원균인 Epicoccosorus nematosporus를 우리나라에서 처음으로 분리, 동정하였으며, 이 병을 올방개 지문무늬병(Finegerprint stem blight disease of water chestnut)으로 명명하였다. 분생포자는 분생자층 위에서 형성이 되며 무격막, 단핵이며, 44.5~72 $\mu\textrm{m}$ 크기의 양끝이 뾰족한 선충 모양이다. 갈색의 병반상에서 흑색의 분생자층은 발달하여 특이한 지문무늬를 형성하는 것이 특징이다. 이 병균은 전국의 올방개가 자라는 논에서 분포하며 매년 발생한다. 온실 접종시 초기의 병징은 접종후 4~5일만에 바늘끝 모양의 회흑색의 반점으로 나타나며 시간이 경과하면서 병반은 급속도로 진전하여 접종후 10~15일 정도 후 약 10mm 내외의 갈색병반이 형성이 되어 올방개 줄기를 감싸고 그 줄기 전체는 고사하게 된다. 분생포자 현탁액(3.2$\times$10\ulcorner conidia/ml)을 온실 조건하에서 분무접종 하였을 때 20일 이내에 약 78.0~93.3% 정도의 고사율을 나타내었다. 올방개 지문무늬병균의 분생포자 현탁액(3.2$\times$10\ulcorner conidia/ml)을 온실 조건하에서 벼 등 31개 작물과 피 등 15개 잡초의 유묘에 접종하였을 때 올방개를 제외한 어떤 식물에도 병원성이 없었다.

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Variability in Virulence of Calonectria ilicicola Isolates on Soybean (콩에 대한 Calonectria ilicicola 균주의 병원성 변이)

  • ;J. s. Russin;J. P. Snow
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.571-577
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    • 1998
  • Variability in virulence of Calonectria ilicicola isolates form different hosts and geographic origin provides important information for breeding cultivars resistant to red crown rot. A wide range in virulence for 25 isolates of C. ilicicola from soybean and peanut was observed on six soybean cultivars. Soybean isolates were pathogenic on soybean although some were less virulent. Virulence of isolates was not affected greatly by cultivar and relatively consistent regardless of virulence level. Soybean isolates were more virulent on soybean than were peanut isolates. When virulence of two soybean and tow peanut isolates was compared between laboratory and greenhouse tests, it was stable across a range of cultivars. Mycelial growth of isolates from either soybean or peanut was reduced significantly on potato dextrose chlorate showed significant reduction of fungal growth than isolates from peanut did although their growth on potato dextrose agar was not significantly different. Evidence for physiologic specialization was not recognized in this system. However, the findings that soybean isolates of C. ilicicola were more virulent on soybean and reduction of fungal growth on potato dextrose chlorate than were peanut isolates suggest that host specialization may exist in this fugus.

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Plant-derived Antibacterial Metabolites Suppressing Tomato Bacterial Wilt Caused by Ralstonia solanacearum

  • Vu, Thuy Thu;Choi, Gyung Ja;Kim, Jin-Cheol
    • Research in Plant Disease
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    • v.23 no.2
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    • pp.89-98
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    • 2017
  • Ralstonia solanacearum species complex (RSSC) causes bacterial wilt, and it is one of the most important soil-borne plant pathogenic bacteria. RSSC has a large host range of more than 50 botanical families, which represent more than 200 plant species, including tomato. It is difficult to control bacterial wilt due to following reasons: the bacterial wilt pathogen can grow inside the plant tissue, and it can also survive in soil for a long period; moreover, it has a wide host range and biological diversity. In most previous studies, scientists have focused on developing biological control agents, such as antagonistic microorganisms and botanical materials. However, biocontrol attempts are not successful. Plant-derived metabolites and extracts have been promising candidates to environmentally friendly control bacterial wilt diseases. Therefore, we review the plant extracts, essential oils, and secondary metabolites that show potent in vivo antibacterial activities (in potted plants or in field) against tomato bacterial wilt, which is caused by RSSC.

STUDIES ON THE VIRUSES OF RADISH MOSAIC

  • KIM, Woon-Soo
    • Journal of Plant Biology
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    • v.6 no.2
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    • pp.9-21
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    • 1963
  • A mosaic diseased radish collected from the suburb of Seoul, in November, 1961 was used for studing the host range, physical properteis, purificaitiion, insect transmission, and electron microscopy. A Japanese strain of radish mosaic(RPV) was also used with Korean strain of radish mosaic (KRMV) for a comparative study. The two viruses, KRMA and RPV, were identified by the difference in host range, insect transmission and electron microscopy. The KRMA was severely infective on tobacco and Nicotiana glutinosa, while on Gomphrena globsa was immune to the virus. RPV produces necrotic local lesions on Gomphrena globosa but did not infect tobacco and N. glutinosa. Among varieties of radish, Seoul, Akamaroo, Akanagea, Koong-Joong showed more severe symptoms than Simoo, Minong, Paek-soo, which appeared to be fainly resistant. In a number of tests, it was found that the virus KRMA retained its infectivity until to a dilution of 1:2,000, heating at $58^{\circ}$ for 10 minutes, adn aging in vitro for 7 days at room temperature. The RPV was not inactivated until it was diluted to 1:2,000, heated to $56^{\circ}$, and aged for 6 days. The KRMV was readily transmitted by the aphid(Myzus persicae Sulz). The virus RPV was not transmitted by the aphid in a number of tests. Partialy purified viruses using ammonium acetate buffer, salting-out by ammonium sulfate and centrifugation of high and low speed were highly infective. Electron micrographs showed that the KRMV paticles are of spherical particles whereas the RPV particles are rod-shaped.

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STUDIES ON THE VIRUSES OF RADISH MOSAIC

  • Kim, Woon Soo
    • Journal of Plant Biology
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    • v.6 no.2
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    • pp.7-7
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    • 1963
  • A mosaic diseased radish collected from the suburb of Seoul, in November, 1961 was used for studing the host range, physical properteis, purificaitiion, insect transmission, and electron microscopy. A Japanese strain of radish mosaic(RPV) was also used with Korean strain of radish mosaic (KRMV) for a comparative study. The two viruses, KRMA and RPV, were identified by the difference in host range, insect transmission and electron microscopy. The KRMA was severely infective on tobacco and Nicotiana glutinosa, while on Gomphrena globsa was immune to the virus. RPV produces necrotic local lesions on Gomphrena globosa but did not infect tobacco and N. glutinosa. Among varieties of radish, Seoul, Akamaroo, Akanagea, Koong-Joong showed more severe symptoms than Simoo, Minong, Paek-soo, which appeared to be fainly resistant. In a number of tests, it was found that the virus KRMA retained its infectivity until to a dilution of 1:2,000, heating at $58^{\circ}$ for 10 minutes, adn aging in vitro for 7 days at room temperature. The RPV was not inactivated until it was diluted to 1:2,000, heated to $56^{\circ}$, and aged for 6 days. The KRMV was readily transmitted by the aphid(Myzus persicae Sulz). The virus RPV was not transmitted by the aphid in a number of tests. Partialy purified viruses using ammonium acetate buffer, salting-out by ammonium sulfate and centrifugation of high and low speed were highly infective. Electron micrographs showed that the KRMV paticles are of spherical particles whereas the RPV particles are rod-shaped.

Genomic Recombination of Bombyx mori and Autographa californica Nuclear Polyhedrosis Viruses (누에 및 Autographa californica 핵다각체병 바이러스에 대한 유전자 재조명)

  • 우수동;박범석;박지현;정인식;양재명;강석권
    • Korean journal of applied entomology
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    • v.32 no.4
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    • pp.407-413
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    • 1993
  • Twelve recombinant viruses with wider host range were plaque purified after coinfectian of Autographa cahjornica and Bombyx mOT! NPVs into Sf9 ar BmN-4 cells. Restriction endonucleases analysis of the recombinant's DNAs showed that the recombinatIOn between AcNPV and BmNPV genomes had occurred more than once. When the recombinam RecB-8, derived from BrnN-4 cells, was observed by electron rntcroscopy, the shape of the polyhedron was a regular tetrahedron, and few virions were occluded into a polyhedron.

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Optimization of the Bacteriophage Cocktail for the Prevention of Brown Blotch Disease Caused by Pseudomonas tolaasii

  • Yun, Yeong-Bae;Um, Yurry;Kim, Young-Kee
    • The Plant Pathology Journal
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    • v.38 no.5
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    • pp.472-481
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    • 2022
  • Brown blotch disease, caused by Pseudomonas tolaasii, is one of the most serious diseases in mushroom cultivation, and its control remains an important issue. This study isolated and evaluated pathogen-specific bacteriophages for the biological control of the disease. In previous studies, 23 varieties of P. tolaasii were isolated from infected mushrooms with disease symptoms and classified into three subtypes, Ptα, Ptβ, and Ptγ, based on their 16S rRNA gene sequences analysis and pathogenic characters. In this study, 42 virulent bacteriophages were isolated against these pathogens and tested for their host range. Some phages could lyse more than two pathogens only within the corresponding subtype, and no phage exhibited a wide host range across different pathogen subtypes. To eliminate all pathogens of the Ptα, Ptβ, and Ptγ subtype, corresponding phages of one, six, and one strains were required, respectively. These phages were able to suppress the disease completely, as confirmed by the field-scale on-farm cultivation experiments. These results suggested that a cocktail of these eight phages is sufficient to control the disease induced by all 23 P. tolaasii pathogens. Additionally, the antibacterial effect of this phage cocktail persisted in the second cycle of mushroom growth on the cultivation bed.

Control Network using Bluetooth with Wire Network (유선 네트워크 기반의 근거리 무선 통신을 이용한 제어용 네트워크)

  • Gwak, Jae-Hyeok;Im, Jun-Hong
    • Proceedings of the KIEE Conference
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    • 2003.11c
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    • pp.476-479
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    • 2003
  • Recently, Bluetooth has been regarded as a new technology for short-range wireless connection. Although initial application of Bluetooth technology has been focused mainly on replacing cables between hand-held devices due to a limited packet size and short-range, general wireless telecommunication such as PAN and Ad hoc networks via Bluetooth-equipped devices is expected to be one of the most popular applications. Wireless equipments have been used to exchange data between host and mobile unit. The exchanging data may be several bytes of control command and the value of sensors with ultra-sonic, vision sensor, and encoder from mobile robot. However, most wireless equipments have some drawbacks such as lack of authentication, large size and high price. On the other hand, the benefits of Bluetooth are small size, low power, low price except short-range. Especially, there are some difficulties when wireless modules are used in indoor environments. In this paper, a method of using wire network in Bluetooth network is investigated as a solution to overcome the short-range problem of Bluetooth and difficulty in indoor environment.

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Improving the Performance of TCP over Wired-Wireless Networks Using the Received Signal Strengths of Mobile Host (이동호스트의 수신신호를 이용한 유무선 혼합망에서의 TCP 성능 향상)

  • Kim, Jin-Hee;Kwon, Kyun-Hee
    • The KIPS Transactions:PartC
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    • v.13C no.5 s.108
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    • pp.635-640
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    • 2006
  • The Snoop in the BS (Base Station) performs a local retransmission over wired-wireless networks to recover packet loss quickly. However, when the MH (Mobile Host) leaves the reception range of the signal, the local retransmission causes performance to degrade. In this paper, we minimize the packet loss and local retransmission caused by the unreachability from BS to MH to improve network performance. To do this, we suggest to add RSS(Received Signal Strengths) flag bit in ACK packet of MH. By using this flag bit, the BS decides whether it retransmits or not to minimize packet loss. The result of the simulation by ns-2 shows a big improvement of performance in the networks.

Eager Data Transfer Mechanism for Reducing Communication Latency in User-Level Network Protocols

  • Won, Chul-Ho;Lee, Ben;Park, Kyoung;Kim, Myung-Joon
    • Journal of Information Processing Systems
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    • v.4 no.4
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    • pp.133-144
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    • 2008
  • Clusters have become a popular alternative for building high-performance parallel computing systems. Today's high-performance system area network (SAN) protocols such as VIA and IBA significantly reduce user-to-user communication latency by implementing protocol stacks outside of operating system kernel. However, emerging parallel applications require a significant improvement in communication latency. Since the time required for transferring data between host memory and network interface (NI) make up a large portion of overall communication latency, the reduction of data transfer time is crucial for achieving low-latency communication. In this paper, Eager Data Transfer (EDT) mechanism is proposed to reduce the time for data transfers between the host and network interface. The EDT employs cache coherence interface hardware to directly transfer data between the host and NI. An EDT-based network interface was modeled and simulated on the Linux-based, complete system simulation environment, Linux/SimOS. Our simulation results show that the EDT approach significantly reduces the data transfer time compared to DMA-based approaches. The EDTbased NI attains 17% to 38% reduction in user-to-user message time compared to the cache-coherent DMA-based NIs for a range of message sizes (64 bytes${\sim}$4 Kbytes) in a SAN environment.