• 제목/요약/키워드: Host range

검색결과 585건 처리시간 0.029초

Construction of a Reporter Strain Pseudomonas putida for the Detection of Oxidative Stress Caused by Environmental Pollutants

  • Lee Yun-Ho;Ahn Eun-Young;Park Sung-Su;Madsen Eugene L.;Jeon Che-Ok;Park Woo-Jun
    • Journal of Microbiology and Biotechnology
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    • 제16권3호
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    • pp.386-390
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    • 2006
  • A green fluorescent protein-based Pseudomonas putida reporter was successfully constructed and shown to be capable of detecting oxidative stress. In this whole-cell reporter, the promoter of the paraquat-inducible ferredoxin-$NADP^+$ reductase (fpr) was fused to a promoterless gfp gene on a broad-host-range promoter probe vector. Pseudomonas putida KT2440 harboring this reporter plasmid exhibited an increased level of gfp expression in the presence of redox-cycling agents (paraquat and menadione), hydrogen peroxide, and potential environmental pollutant chemicals such as toluene, paint thinner, gasoline, and diesel. Induction of fpr in the presence of these chemicals was confirmed using Northern blot analysis.

lux Operon과 Heat Shock Promoter 유전자 재조합을 통한 독성물질 탐지용 대장균의 개발 (Construction of Bioluminescent Escherichia coli from lux Operon and Heat Shock Promoter for the Detection of Toxic Substances)

  • 유승오;이은관;김현숙;정계훈;전억한
    • 한국미생물·생명공학회지
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    • 제27권4호
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    • pp.278-285
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    • 1999
  • In order to use heat shock promoter for the detection of toxic substances, dnaK promoter was amplified from E. coli genomic DNA by using a polymerase chain reaction(PCR) followed by sequencing and sub-cloning into the multi-cloning site of the plasmid, pUCD615. The pUCD615 is a broad-host-range vector containing promoterless lux operon originated from V.fischeri. The recombinant plasmid was transfered to E. coli DH5$\alpha$ through electroporation. The recombinant E. coli showed several patterns of bioluminescent responses to ethanol stress. The bioluminescent E. coli also showed responses to other toxic substances including FeK3(CN)6, CdCl2, p-nitrophenol and HgCl2. The increases of RLU(Relative Light Unit) were observed at 100ppm of FeK3(CN)6, 10ppm and 100ppm and 100ppm of CdCl2, 1ppm of 10ppm of p-nitrophenol and at 1ppm of HgCl2.

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Synthesis of Host Polymers and Guests for Electrophosphorescence

  • Holmes Andrew B.;Chan, Khai-Leok;Cho, Sung-Yong;Evans Nicholas R.;Grimsdale Andrew C.;Mak Chris S.K.;Sandee Albertus J.;Watkins Scott E.;Williams Charlotte K.
    • 한국고분자학회:학술대회논문집
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    • 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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    • pp.21-22
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    • 2006
  • Significant progress has been realized in the design and synthesis of light emitting polymers with emission over the whole range of the visible spectrum. However up to seventy-five percent of charge recombination events can lead to triplet states that decay non-radiatively. Following the pioneering work in the field of small molecule organic light emitting devices it has been found that solution processible iridium polymer complexes can be used to harness the wasted triplet energy. In this paper new results concerning electrophosphorescence of solution processible tethered iridium polymer derivatives will be presented. Furthermore our approaches to the design of new high triplet energy conjugated polymer hosts will be reported.

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한국형전술데이터링크(Link-K)에서 TDL 프로토콜과 IP의 효과적인 적용방안 분석 (An Analysis for Efficient Appliance of TDL Protocol and IP on Link-K System)

  • 지승배
    • 한국군사과학기술학회지
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    • 제11권1호
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    • pp.25-32
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    • 2008
  • In this paper, we propose how to efficiently apply IP and TDL(Tactical Datalink) protocol to the Link-K network for using their applications on the host system. We classify 3 kinds of communication method between IP network and TDL network. We explain the concepts of Like-to-Like communication (Link-K over IP, IP over Link-K) and Any-to-Any communication. We describe the characteristics and the implementation range of tactical datalink processor for each communication method.

Preparation and Luminescence Properties of $LaPO_4:Eu/:LaPO_4$ Core/Shell Nanoparticle

  • Oh, Jae-Suk;Seok, Sang-Il;Jung, Ha-Kyun
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.II
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    • pp.1495-1498
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    • 2005
  • Due to an efficient red emission, $Eu^{+3}$ ions have doped in various host materials. Eu-doped $LaPO_4/LaPO_4$ core/shell nanophosphor which emits red light in visible range, was synthesized by using a solution system. The mean size of the nanoparticle observed by TEM was about 7-8 nm. In this core/shell system, the thickness of $LaPO_4$ shell was optimized on the basis of relative PL emission intensity. In addition, the luminescent properties of $LaPO_4:Eu/LaPO_4$ core/shell particle have been compared with Eu-doped $LaPO_4$ bulk particle and nanoparticle.

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Synthesis and Mesomorphic Properties of Achiral Liquid Crystals with 1,3-Dialkoxy-2-propyl Swallow-Tail

  • Lee, Seng-Kue;Bang, Mi-Yeon;Lee, Jong-Gun;Kang, Kyung-Tae;Kim, Yong-Bae
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2003년도 International Meeting on Information Display
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    • pp.575-577
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    • 2003
  • Achiral swallow-tailed liquid crystals derived from 1,3-dialkoxy-2-propanol were prepared and their mesomorphic properties were investigated. 1,3-Dialkoxy-2-propyl swallow-tailed material showed antiferroelectric-like smectic C phase at lower temperature and in broader temperature range than the corresponding compounds with a branched alkyl group as a swallow-tail. They could serve as suitable host for antiferroelectric mixtures.

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Active Matrix OLED Displays with High Stability and Luminous Efficiency by New Doping Method

  • Shibata, Kenichi;Hamada, Yuji;Kanno, Hiroshi;Takahashi, Hisakazu;Mameno, Kazunobu
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2003년도 International Meeting on Information Display
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    • pp.4-6
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    • 2003
  • We have developed the active matrix OLED displays with a high efficiency red emission material which uses an emitting assist (EA) dopant system. The EA dopant (rubrene) did not itself emit but assisted the energy transfer from the host ($Alq_s$) to the red emitting dopant(DCM2). A stable red emission (chromaticity coordinates: x=0.64, y=0.36) was obtained in this cell within the luminance range of 100 - 4000 $cd/m^2$ By using EA dopant system, we can realize the reduction of the power consumption of the OLED display..

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ARCA-An Adaptive Routing Protocol for Converged Ad-Hoc and Cellular Networks

  • Wu, Yumin;Yang, Kun;Chen, Hsiao-Hwa
    • Journal of Communications and Networks
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    • 제8권4호
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    • pp.422-431
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    • 2006
  • This paper proposes an adaptive routing protocol called ARCA for converged ad-hoc and cellular network (CACN). Due to the limitation of both bandwidth and transmission range in a cell, a mobile host (MH) may not be able to make a call during busy time. CACN offers a flexible traffic diversion mechanism that allows a MH to use the bandwidth in another cell to ease the congestion problem and increase the throughput in a cellular network. Based on the presentation of CACN's physical characteristics, the paper details the design issues and operation of the adaptive routing protocol for CACN (ARCA). Detailed numerical analysis is presented in terms of both route request rejection rate and routing overhead, which, along with the simulation results, have indicated the effectiveness and efficiency of the ARCA protocol.

대만에서 고추에 발생한 미보고 Potyvirus에 관한 연구 (An Unusual Potyvirus from Pepper in Taiwan)

  • 김정수
    • 한국식물병리학회지
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    • 제3권4호
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    • pp.261-269
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    • 1987
  • 포장에서 고추에 황화, 엽맥록대 그리고 엽맥주름을 일으키는 바이러스가 분리되었다. 이 바이러스는 PVY항혈청과 ELISA 검정에서 반응하였으나 CMV, TMV, TBRV, AMV, TSWV, TEV, PMV, TRSV와는 반응하지 않았다. 바이러스 입자의 전자현미경 검경 결과 길이가 750-760nm의 사상형 입자였다. 이 바이러스는 진딧물에 의해 쉽게 전염되었으며 기주범위는 PVY와 비슷하였으나 Chemopodium amaranticolor와 C. quinoa에 전신 감염을 일으켰다.

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오이 모자이크 바이러스와 수눔 모자이크 바이러스의 복합감염에 의한 스톡의 꽃잎얼룩무늬병 (Color Breaking Syndrome of Matthiola incana Caused by Double Infection of Cucumber Mosaic Virus and Turnip Mosaic Virus)

  • 윤주연;최홍수;류화영;함영일;최장경
    • 한국식물병리학회지
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    • 제14권3호
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    • pp.220-222
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    • 1998
  • In 1995, we collected stock (Matthiola incana) plants causing mosaic symptoms on leaves and color breakings on flowers in Daekwallyong, Korea. Two viruses were isolated from the infected plants, and identified as cucumber mosaic virus (CMV) and turnip mosaic virus (TuMV) by experiments of host range, serology and electron microscopy. Each of the virus did not produce the same symptoms on the stock seedlings as naturally infected plants caused. When the viruses were coinoculated to the stock seedlings, however, severe mosaic symptoms were observed on leaves, and then the color breakings were expressed on flowers.

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