• 한국버섯학회지
• /
• 제15권3호
• /
• pp.134-138
• /
• 2017

양송이의 국내 생산량은 9,732톤으로 5번째로 가장 많이 생산되는 버섯이다. 양송이가 속한 주름버섯속은 리그노셀룰로오스 분해력을 가진 버섯으로 알려져 있다. 양송이의 영양생장과 생식생장동안 기본적으로 셀룰로오스, 해미셀룰로오스, 리그닌 성분의 분해는 변화한다. 우리는 양송이에서 celluase, xylanase, ligninolytic enzyme의 세포외효소 활성도로 효과적인 생물학적 분해력을 가진 균주를 선발하였다. 각 효소의 생물학적 분해는 0.5% CMC-MMP ((malt-mops-peptone), 0.5% Xylan-MMP, 0.5% ligin-MMP 배지에서 14일 동안 균주들을 배양하여 측정하였다. 그리고 배양된 균사체는 0.2% trypan blue에 염색되었다. MMP배지내 각 효소의 생물학적 분해의 효율성에 따라 선발된 균주 18점은 6개로 그룹화되었다. 또한 이 균주들은 볏짚발효퇴비배지에서의 균사생장과 세포외효소 활성도의 연관성을 알아보기 위해 볏짚발효퇴비배지에서 10일, 20일 각각 배양되었다. 본 연구로 ligninolytic enzyme 활성도가 볏짚발효퇴비배지에서의 균사배양과 상관도가 가장 높아 균주내 ligninolytic enzyme의 활성도 비교로 볏짚발효퇴비배지에서 균사배양이 우수한 균주를 간단하게 선발 할 수 있다고 사료된다.

• Journal of Plant Biotechnology
• /
• 제47권1호
• /
• pp.90-99
• /
• 2020

This study aimed to screen for plants with antimicrobial potential among the evergreen woody species of South Korea that are used for horticulture and landscaping and to provide basic information about plants with proven antimicrobial activity to underpin future research. The plant materials were extracted under various conditions, and the antimicrobial activities of the extracts were evaluated by agar diffusion assay. The screening tests demonstrated that the crude extracts of 43 species had inhibitory effects against S. aureus. The inhibitory activities of four species (Elaeocarpus sylvestris, Camellia japonica, Cleyera japonica, and Quercus salicina) were relatively higher than that of the synthetic antimicrobial agents methylparaben and phenoxyethanol. The highest inhibitory activity was observed with the leaf extracts (extracted with methanol for 30 minutes) of E. sylvestris, based on induction of the largest inhibition zone of 23.3 mm in size. In addition, solvent fractions of E. sylvestris were evaluated. The largest inhibitory zone of 23.1 mm was observed for the n-butanol fraction, which is likely to contain effective compounds that exhibit inhibitory activity against S. aureus. In contrast, n-hexane and residual aqueous fractions showed no antimicrobial activity. Overall, our findings confirm that evergreen woody plants native to South Korea have potential antimicrobial activity.

• 한국토양비료학회지
• /
• 제44권5호
• /
• pp.801-807
• /
• 2011

유기물 연용이 감자재배 토양의 토양화학성과 효소활성에 미치는 영향을 평가하고자 비화산 암갈색 토양에서 1년 2기작으로 3 작기 동안 감자재배를 하였다. 유기물은 작기당 유기질비료와 음식물퇴비는 $2,000kg\;10a^{-1}$, 돈분퇴비는 2,000, 4,000, and $6,000kg\;10a^{-1}$을 기준으로 시용하였다. 토양 pH는 3 작기 후 모든 처리구에서 높았다. PMC 시용량이 많을수록 토양 pH, 토양질소, 유효인산, 치환성 양이온 (칼륨, 칼슘 및 마그네슘), Zn, Cu 함량이 증가하였다. 토양 탈수소효소활성은 PMC 시용량이 많고 작기가 증가할수록 대조구 보다 3작기 후는 PMC 4톤구가 2배 이상 높았으며, PMC>FWC>NPK+PMC>MOF 순으로 증가하였다. 토양인산효소활성은 PMC 6톤구에서 가장 높았다. 3작기 동안 유기물 처리구의 Zn함량이 증가함에 따라 탈수소효소 활성이 직선적으로 증가하였으며 MOF구 $R^2$=0.427, FWC 구 $R^2$=0.427, PMC구 $R^2$=0.411 (p<0.01)의 상관관계를 나타냈다.

• 식물병연구
• /
• 제20권4호
• /
• pp.264-269
• /
• 2014

시설오이와 상추 재배지에서 녹비작물의 종류에 따른 M. incognita의 생물학적 방제효과를 조사하였다. 시설오이 재배지에서 수단그라스 재배는 fosthiazate GR의 화학적 처리 효과와 유사한 88.6%의 살선충 효과를 보였다. 상추재배지에서 수단그라스의 재배는 토양중 93.5%의 M. incognita 밀도 감소를 보였다. 또한 수단그라스는 다른 녹비작물보다 매우 우수한 생육을 보였기 때문에 시설 재배지에서 M. incognita를 관리하는데 수단그라스가 적당한 것으로 생각된다.

• 성인간호학회지
• /
• 제13권3호
• /
• pp.409-419
• /
• 2001

The reference study was performed to investigate the nursing importance which was based a theoretical background related to horticultural therapy and to examine the possibility which horticultural therapy was applicable as a nursing intervention through analysis on a preceding study. The research subjects related to the areas of horticultural therapy which related as a nursing intervention, were psychological, physical, environmental, and psycho-social aspects. It is judged that the horticultural therapy is effective in treating depression, emotional disruption or anxiety. It seems to be also effective in increasing the muscular tension and, thereby, expanding the scope of joint movements. Such theories show that horticultural therapy may be a good alternative nursing means. Plants act to create a pleasant interior atmosphere by generating anion, controlling the temperature and humidity and purifying the air, and therefore, the horticultural therapy may be applied to clinic or environmental therapy. When horticultural therapy is used as a nursing intervention, patients' sociopsychological needs may be fulfilled. It has been found that horticultural therapy is instrumental in treating perceptive or emotional disruption, depression, loss of self-respect, disrupted everyday activities and social behaviors. In particular, horticultural therapy seems to be effective in managing chronic patients' crisis or improving life quality. Intervention method applied on a preceding study was activity therapy and scene therapy in the horticultural therapy The above findings suggest that the pro-environmental horticultural therapy is useful as a new paradigm of nursing or holistic nursing conducive to improvement of health. So, it is desirable to prove its effects by applying it in the clinic. In order to apply horticultural therapy as a nursing intervention, it will be necessary to educate nurses on principles and methods of horticultural therapy and encourage them to apply it in nursing and clinics. Moreover, it may well be necessary to develop landscape therapy as nursing intervention as well as a variety of horticultural therapy programs befitting the clinic conditions. On the other hand, we need to provide for some scientific ground for horticultural therapy through continued studies. In this regard, this study which focuses on patients' health improvement through change of environment. may well provide for a framework for such studies.

• 한국약용작물학회:학술대회논문집
• /
• 한국약용작물학회 2011년도 추계학술발표회
• /
• pp.264-265
• /
• 2011

• 원예과학기술지
• /
• 제30권6호
• /
• pp.769-775
• /
• 2012

Among the phenolic compounds that is generally present in strawberry fruits, five simple phenolics, three flavonoids, and a stilbene were tested for their antimicrobial activity against seven fungi and one oomycete. trans-Cinnamic acid showed strong antimicrobial activity, and the antimicrobial effect of the simple phenolics decreased with an increase in the number of hydroxyl groups. Phytophthora capsici was the most susceptible to the phenolic compounds tested in this study. trans-Cinnamic acid, p-hydroxybenzoic acid, and kaempferol were mainly detected in 'Seolhyang' strawberry fruits, and the total phenolic contents of the fruits decreased during their development. Extracts of the green (1-10% red color) and red (above 90% red color) strawberry fruits reduced the mycelial growth and zoospore germination rate of P. capsici, and the extract of red strawberry fruit showed strong antimicrobial activity against the zoospore germination of P. capsici. These results indicate that strawberry fruits contain antimicrobial phenolic compounds and that strawberry fruit extract can be used as a natural fungistat.

• 한국버섯학회지
• /
• 제7권4호
• /
• pp.168-172
• /
• 2009

We attempted to isolate the bacterial strains from the fruiting bodies of Agaricus brazilensis and determined their effects on browning and distortions of mushrooms. No bacterial strains were isolated when the middle of browning regions of A. brazilensis were used. Total 125 bacterial strains were obtained from the surface of browning regions and classified into 17 different genera and 29 species by using MIDI methods. Most common genus were Pseudomonas (26), Yersinia (29), and Cedecea (29). High lytic activity were detected when Pseudomonas strains were tested, while relatively low lytic activity were observed with both Yersinia and Cedecea strains.Therefore, we believed the distortion of mushroom could be the result of bacterial infections. Also, the development of brownish color was detected in large number of A. brazilensis strains only by incubation at 4C, suggesting no specific correlation between bacterial strains and brownish color development. Also, it is considered that the development of brownish color can be the normal changes of A. brazilensis.

• Journal of Ginseng Research
• /
• 제37권4호
• /
• pp.442-450
• /
• 2013

The aim of this study was to determine and compare the preventive effect of Korean White Ginseng and Red Ginseng on oxidative stress in $H_2O_2$-treated HepG2 cells. The roots of ginseng were extracted with 70% methanol and partitioned with butanol to obtain saponin fractions, which have been known as bioactive constituents of ginseng. 2',7'-Dichlorofluorescein diacetate (DCF-DA) assay and malondialdehyde (MDA) content were measured for evaluating intracellular reactive oxygen species (ROS) generation. Also, mRNA expressions and activities of antioxidant enzymes were analyzed to determine the antioxidant activity of saponin or non-saponin fractions of ginsengs. According to DCF-DA assay, $H_2O_2$-induced MDA release and ROS generation were significantly reduced by treatment with saponin fractions of white and red ginseng roots. Also, saponin fractions increased effectively intracellular antioxidant enzyme activities including catalase, glutathione peroxidase and superoxide dismutase in $H_2O_2$-treated HepG2 hepatoma cells. In general, red ginseng was more effective than white ginseng for reducing oxidative stress. These results indicate that administration of red ginseng may certainly contribute relatively stronger than white ginseng to prevent from damaging liver function by oxidative stress.

• 인간식물환경학회지
• /
• 제22권2호
• /
• pp.145-157
• /
• 2019

This study was conducted to examine the effects of horticultural therapy on depression and emotional balance of women with hearing impairment. Ten women with hearing impairment registered in the Mokpo branch of the Korean Association of the Hearing Impaired were selected, and participated in the horticultural therapy program composed of 14 sessions from May 15 to July 31. The horticultural therapy program consisted of 'planting tropical orchids,' 'making a dish garden,' 'making plum pickles,' 'decorating a table with flowers,' 'making a hanging pot with succulent plants,' 'making pressed flowers with hydrangea,' 'making a fan with pressed flowers,' 'making a terrarium,' 'making a flower basket,' 'making a mosquito repellent spray,' 'decorating a heart-shaped cup with flowers,' 'making a natural herbal soap,' 'making a hydrangea bouquet' and 'making watermelon salad.' The depression and emotional balance scales were used to examine the effects of the program and the pre- and post-program scores were compared. The mean value of depression statistically significantly decreased from 49.0 points before the program to 43.9 points after the program. In addition, the mean value of emotional balance statistically significantly increased from 0.3 points before the program to 2.3 points after the program. The activity of drawing a picture after each session was effective in reducing depression and improving positive emotional balance by expressing emotions through pictures. The programs preferred by women with hearing impairment included 'making plum pickles,' 'making pressed flowers with hydrangea,' 'planting tropical orchids,' 'making a mosquito repellent spray,' 'making a natural herbal soap,' and 'making watermelon salad.' In conclusion, horticultural therapy programs were effective in reducing depression and improving emotional balance of women with hearing impairment.