• Title/Summary/Keyword: Hornet

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Bioenvironmental Interaction of Toxic Peptide Hornet Venom with Phospholipid (Hornet 독액의 독성 Peptide와 Phospholipid 간의 생체환경적 상호작용)

  • 김광호;이봉헌
    • Journal of Environmental Science International
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    • v.6 no.2
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    • pp.189-194
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    • 1997
  • Toxic peptides from hornet venom, mastoparan and mastoparan-B were synthesized us- ing the solid phase peptide synthesis method and investigated the interaction of them with phospholipid bilayer, antibacterial activity, and hemolytic activity. Both toxic peptides could induce dye release at a low concentration in neutral liposome. The binding affinity of mastoparan-B for neutral liposome was smaller than that for acidic one. Mastoparan and mastoparan-B had strong antibacterial activity for gram-positive bacteria, but weak or potent activity for gram-negative ones, respectively. Mastoparan and mastoparan-B lysed erythrocyte very little up to 5 $\mu$M.

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Prototype Design of Hornet Cloud using Virtual Honeypot Technique (가상 허니팟 기술의 호넷 클라우드의 프로타입 설계)

  • Cha, Byung-Rae;Park, Sun;Kim, Jong-Won
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.8
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    • pp.891-900
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    • 2015
  • Cloud Computing has recently begun to emerge as a new attack target. The malice DDoS attacks are ongoing to delay and disturb the various services of the Cloud Computing. In this paper, we propose the Hornet-Cloud using security Honeypot technique and resources of Cloud Computing, and design the concept of active-interaction and security functions of Hornet-Cloud simply.

Attraction of the Invasive Hornet, Vespa velutina nigrithorax, by using Bacillus sp. BV-1 Cultures

  • Lim, Da Jung;Lee, Jeong Eun;Lee, Jin Sil;Kim, Iksoo;Kim, In Seon
    • Korean Journal of Environmental Agriculture
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    • v.38 no.2
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    • pp.104-109
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    • 2019
  • BACKGROUND: The invasive hornet Vespa velutina nigrithorax has becomes a public concern in rural and urban South Korea. The technologies are necessary to develop a way to counter V. velutina. In an effort to develop a way to counter V. velutina, we found that a bacillus strain, named Bacillus sp. BV-1, produces volatile compounds that attract V. velutina. METHODS AND RESULTS: Field trials of V. velutina attraction were performed using plates and traps containing BV-1 cultures grown on sugar medium. When the sugar medium and sugar-grown BV-1 cultures in the plates were placed close together, V. velutina visited preferably the plates with the BV-1 cultures. Significantly more V. velutina were caught in the traps containing BV-1 cultures than in those containing only sugar medium. Headspace solid-phase microextraction coupled with GC/MS analysis of BV-1 cultures detected 2-methyl-1-propanol, 3-methyl-1-butanol, 3-methylbutanoic acid, ethyl hexanoate, 2-pheylethanol, ethyl octanoate, and ethyl decanoate as the major volatiles. CONCLUSION: BV-1 cultures were suggested as potential agents for managing V. velutina as they produce volatile compounds that attract the hornet.

Real Time Hornet Classification System Based on Deep Learning (딥러닝을 이용한 실시간 말벌 분류 시스템)

  • Jeong, Yunju;Lee, Yeung-Hak;Ansari, Israfil;Lee, Cheol-Hee
    • Journal of IKEEE
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    • v.24 no.4
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    • pp.1141-1147
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    • 2020
  • The hornet species are so similar in shape that they are difficult for non-experts to classify, and because the size of the objects is small and move fast, it is more difficult to detect and classify the species in real time. In this paper, we developed a system that classifies hornets species in real time based on a deep learning algorithm using a boundary box. In order to minimize the background area included in the bounding box when labeling the training image, we propose a method of selecting only the head and body of the hornet. It also experimentally compares existing boundary box-based object recognition algorithms to find the best algorithms that can detect wasps in real time and classify their species. As a result of the experiment, when the mish function was applied as the activation function of the convolution layer and the hornet images were tested using the YOLOv4 model with the Spatial Attention Module (SAM) applied before the object detection block, the average precision was 97.89% and the average recall was 98.69%.

Interaction of Hornet Venom and its Derivatives in the Phospholipid Membrane Environment (말벌 독성 물질과 그 유도체의 인지질 막 환경에서의 상호작용)

  • 이봉헌;박홍재
    • Journal of Environmental Science International
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    • v.7 no.1
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    • pp.62-66
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    • 1998
  • Toxic Mastoparan B(MP-B) which is purified from the venom of the hornet Vespa basalis is a cationic amphlphilic tetradecapeptide. MP-B and Its Ala-substituted analogues were synthesized by solld phase method and the toxic peptide-membrane interactions were examined by circular dichroism(CD) spectra, fluorescence spectra, and leakage abilities in phospholipid membranes. In the presence of phospholipid vesicles, synthetic MP-B and its analogues formed amphiphilic -helical structures, but in the buffer soletion, those exhibited random coil conformation as measured by CD. Fluorescence spectra of MP-B and its analogues which indicated the binding affinity of peptide on phospholipid vesicles showed that the replacement of Lys at position 2 and 11 with Ala caused a remarkable effect in the blue shalt and that at position 2, in the leakage ability of the peptide.

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Development of an Ultra Light Flying Device for Remove the Vespiary (말벌 집 제거용 초경량 비행장치의 개발)

  • Geun-Chul Lee;Kyong-Jin Park
    • Journal of the Korean Society of Industry Convergence
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    • v.26 no.3
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    • pp.395-400
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    • 2023
  • Recently, the number of hornet is rapidly increasing because of change in the ecological climate. It gives a sense of threat to the people and damages the beekeeping industry as a predator of honey bees. Firefighters are working to remove hornet and vespiary, but it has been reported that it is difficult to remove at high altitudes. In this study, an ultra-light flying device was developed to remove vespiary. It has been developed in four types: Drug spray type, Clamp type, Cutter type, and Scraper type, which can be used as field-customized equipment. Therefore, the developed equipment is expected to contribute to the safe field activities of firefighters.

Cyber-Resilience-based Virtual Honeypot Service: Framework Sketch and Feasibility Verification (사이버 탄력성 기반 가상 허니팟 서비스 프레임워크 구상 및 가능성 검증)

  • Cha, Byung Rae;Park, Sun;Kim, Jong Won
    • Smart Media Journal
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    • v.5 no.2
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    • pp.65-76
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    • 2016
  • Cloud Computing has recently begun to emerge as a new attack target. The malice DDoS attacks are ongoing to delay and disturb the various services of the Cloud Computing. In this paper, we propose the Hornet-Cloud using security Honeypot technique and resources of Cloud Computing, define and design the concept of security functions about active low-interaction framework by cyber resilience simply. In addition, for virtual honeypot service, we simulated and vitrified the possibility of functions of the low-interaction vHoneypot using cyber resilience.

The Interaction of Mastoparan B from Venom of a Hornet Vespa Basalis with Phospholipid Matrices

  • 박남규;Yuhji Yamato;Sannamu Lee;Gohsuke Sugihara;박장수;강신원
    • Bulletin of the Korean Chemical Society
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    • v.17 no.3
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    • pp.239-244
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    • 1996
  • Mastoparan B (MP-B) that is a novel MP isolated from the hornet Vespa basalis, was studied as compared with MP, in terms of interaction with phospholipid bilayer and antimicrobial activity. MP-B has more hydrophilic amino acid residues in hydrophilic face of amphiphilic α-helical structure than MP. The both peptides exhibited considerably different effect on interaction with lipid bilayers, e.g. their conformation in the presence of acidic and neutral liposomes, dye-release ability from encapsulated liposomes, but on the whole the interaction mode was similar. On antimicrobial activity, MP had a strong activity against Gram-positive bacteria but no against Gram negative ones. Contrary to this, MP-B had a strong activity against Gram-positive and potent against Gram-negative ones. Since both peptides have almost same residues on the hydrophobic side, such more hydrophilic surface on the molecule seems to lead to the subtle change in its interaction with membranes, resulting in the alternation in its biological activity.