• International Journal of Stem Cells
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• 제16권4호
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• pp.438-447
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• 2023

Recently, ex-vivo gene therapy has emerged as a promising approach to enhance the therapeutic potential of mesenchymal stem cells (MSCs) by introducing functional genes in vitro. Here, we explored the need of using selection markers to increase the gene delivery efficiency and evaluated the potential risks associated with their use in the manufacturing process. We used MSCs/CD that carry the cytosine deaminase gene (CD) as a therapeutic gene and a puromycin resistance gene (PuroR) as a selection marker. We evaluated the correlation between the therapeutic efficacy and the purity of therapeutic MSCs/CD by examining their anti-cancer effect on co-cultured U87/GFP cells. To simulate in vivo horizontal transfer of the PuroR gene in vivo, we generated a puromycin-resistant E. coli (E. coli/PuroR) by introducing the PuroR gene and assessed its responsiveness to various antibiotics. We found that the anti-cancer effect of MSCs/CD was directly proportional to their purity, suggesting the crucial role of the PuroR gene in eliminating impure unmodified MSCs and enhancing the purity of MSCs/CD during the manufacturing process. Additionally, we found that clinically available antibiotics were effective in inhibiting the growth of hypothetical microorganism, E. coli/PuroR. In summary, our study highlights the potential benefits of using the PuroR gene as a selection marker to enhance the purity and efficacy of therapeutic cells in MSC-based gene therapy. Furthermore, our study suggests that the potential risk of horizontal transfer of antibiotics resistance genes in vivo can be effectively managed by clinically available antibiotics.

• 대한치과교정학회지
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• 제23권4호
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• pp.619-631
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• 1993

Tooth movement by segment is one of the means which are frequently used in daily orthodontic practice. When we retract or intrude a tooth or teeth, we should recognize the center of resistance of the certain tooth or teeth. There have been many studies about the center of resistance of a single tooth, not so much was about the tooth-segment. At the present study the center of resistance of the maxillary anterior segment is experimentally investigated by using laser reflection technique and metal splints on the human dry skull. The variables of intrusive force magnitude are divided into two groups, 50g and 100g groups. The results were as follows ; 1. The center of resistance of the maxillary anterior segment composed of the central and lateral incisors was at the mesial portion of canine crown at the coronal level. 2. The center of resistance of the maxillary anterior segment composed of the central and lateral incisors and canines is between the canine and the 1st premolar crowns at the coronal level.

• Earthquakes and Structures
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• 제24권3호
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• pp.205-215
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• 2023

Currently, many studies are underway at home and abroad on the seismic performance evaluation and dry construction method of the masonry structure. In this study, a dry stack masonry wall system without mortar using concrete blocks is proposed, and investigate the seismic performance of dry filling wall frames through experimental studies. First, two types of standard blocks and key blocks were designed to assemble dry walls of concrete blocks. And then, three types of experiments were manufactured, including pure frame, 1/2 height filling wall frame, and full height filling wall frame, and cyclic load experiments in horizontal direction were performed to analyze crack patterns, load displacement history, rebar deformation yield, effective stiffness change, displacement ductility, and energy dissipation capacity. According to the experimental results, the full height filling wall frame had the largest horizontal resistance against the earthquake load and showed a high energy dissipation capacity. However, the 1/2 height filling wall frame requires attention because the filling wall constrains the effective span of the column, limiting the horizontal displacement of the frame. In addition, the concrete block was firmly assembled in the vertical direction of the wall as the horizontal movement between the concrete blocks was allowed within installation margin, and there was no dropping of the assembled concrete block.

• 한국지반환경공학회 논문집
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• 제21권7호
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• pp.5-16
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• 2020

현재 경주지진 및 포항지진 발생으로 내진설계기준이 강화되어 기존 시설물에 대한 내진성능평가를 실시하고 있다. 기존 시설물의 피해를 최소화하면서 국한된 협소한 장소에서 시공이 가능한 마이크로파일공법을 개선하여 지진 시 내진성능효과를 확보하고자 한다. 개선방법은 주 기둥인 연직말뚝 주변에 우산형태로 경사말뚝을 시공하여 말뚝 상부의 사각접시형 플레이트에서 말뚝을 모두 일체화하는 것이다. 본 논문에서는 수치해석으로 사질토지반에서 다양한 지진파에 대해 우산형 마이크로파일의 수평변위 거동을 분석하였다. 수치해석 결과, 지반이 연약할수록 우산형 마이크로파일의 수평저항력의 효과가 우수하였다. 경사말뚝의 근입심도에 따른 분석결과, 동일한 지반강도에서 근입심도가 15m 이상일 경우에 수평변위 저감 효과가 뚜렷했으며, N치 30 이상의 지반에 정착하면 지진 시에 효과가 있는 것으로 확인되었다. 마이크로파일의 근입심도와 수평변위 억제효과가 비례하였으며, 대체적으로 지반이 약할수록 변위억제 효과가 컸다. 우산형 마이크로파일은 수직말뚝이 모멘트에 대한 저항을 하고, 경사말뚝이 축력에 대한 저항을 하는 복합저항효과가 있었다.

• 수산해양기술연구
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• 제35권3호
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• pp.335-342
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• 1999

The purpose of present research is to develop and efficient numerical method for the calculation of potential flow and predict the wave-making resistance for the application to ship design of tuna purse seiner. Havelock was considered the wave resistance of a post extending vertically downwards through the water from the surface, its section by a horizontal plane being the same at all depths and having its breadth small compared with its length. This enables us to elucidate certain points of interest in ship resistance. However, the ship has not infinite draft. So, the problem which is investigated ind detail in this paper is the wave resistance of a mathematical quadratic model in a uniform stream. The paper deals with the numerical calculation of potential flow around the series 60 with forward velocity by the new slender ship theory. This new slender ship theory is based on the asymptotic expression of the Kelvin-source, distributed over the small matrix at each transverse section so as to satisfy the approximate hull boundary condition due to the assumption of slender body. The numerical results using the panel shift method and finite difference method are compared with the experimental results for wigley mono hull. There are no differences in the wave resistance. However, it costs much time to compute not only wave resistance but also wave pattern over some range of Froude numbers. More improvements are strongly desired in the numerical procedure.

• 한국화재소방학회논문지
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• 제25권5호
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• pp.76-84
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• 2011

건축물 화재는 인명과 재산피해가 동시에 유발될 수 있는 인위 재해이며, 피해의 최소화하기 위해서는 건축물 설계단계부터 화재안전에 관한 요구사항 특히 내화성능을 충족해야만 한다. 강구조물에서의 보부재는 수평하중 전달과 바닥하중을 축부재로 전달하는 주요 구조부재이며, 주로 H형강으로 시공되고 있다. H형강의 내화성능은 내화피복 시공에 의해서 만족되고 있으나, 구조성능과 내화성능을 동시에 향상시킬 수 있는 콘크리트 충전 보부재의 내화성능에 관한 연구 그리고 작용 하중비에 따른 내화성능 연구는 미진한 실정이다. 따라서 본 논문에서는 H형강 보부재와 H형강 웨브에 보통강도 및 고강도 콘크리트가 충전된 H형강 보부재를 대상으로 내화성능과 하중비에 따른 한계온도 자료를 확보하여 향후 성능적 내화설계의 기초자료로 활용하고자 한다.

• 한국농공학회지
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• 제42권4호
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• pp.115-123
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• 2000

This study compared the degree of consolidation by hyperbolic, curve fitting , Asaoka's and methods using values measured with a theoretical curve in consideration of smear effect and well resistance. The degree of consolidation by the Hyperboilc method was underestimated than the degree of consolidation by Curve fitting. Asaoka's , and Monden's methods. The typical range of the coefficient of horizontal consolidation was Ch=(2-3)Cv in the case considering smear effect and well resistance, and Ch =(0.5-2.1) Cv in the case disregarding smear effect and well resistance. The degree of consolidation obtained by ground settlement monitoring was nearly the same value when the coefficient of smear zone permeability by back analysis was shown to be half that of in-situ and the diameter of the smear zone was shown to be double that of mandrel. By increasing the diameter reduction ratio of the drain, the time of consolidation was delayed. The effect of well resistance showed that the case of a small coefficient of permeability was much more than in the case of a large coefficient of permeability . It was recommended that when designing diameter reduction of a drain, well resistance should be considered.

• Journal of Mechanical Science and Technology
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• 제19권6호
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• pp.1338-1346
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• 2005

Effects of thermal contact resistance between heater and susceptor, susceptor and graphite board in a MOCVD reactor on temperature distribution and film growth rate were analyzed. One-dimensional thermal resistance model considering thermal contact resistance and heat transfer area was made up at first to find the temperature drop at the surface of graphite board. This one-dimensional model predicted the temperature drop of 18K at the board surface. Temperature distribution of a reactor wall from the three-dimensional computational fluid dynamics analysis including the gap at the wafer position showed the temperature drop of 20K. Film growth rates of InP and GaAs were predicted using computational fluid dynamics technique with chemical reaction model. Temperature distribution from the three-dimensional heat transfer calculation was used as a thermal boundary condition to the film growth rate simulations. Temperature drop due to the thermal contact resistance affected to the GaAs film growth a little but not to the InP film growth.

• 한국산학기술학회논문지
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• 제22권6호
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• pp.267-274
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• 2021

말뚝의 허용횡방향지지력을 결정하는 방법으로는 지반반력법과 극한횡방향지지력에 근거한 방법이 많이 쓰이고 있는데 설계시 지반반력법에 근거한 방법만을 적용하는 경우가 있다. 본 연구에서는 지반조건과 말뚝머리 구속조건 그리고 말뚝길이에 따른 말뚝의 허용지지력을 이들 두 가지 방법을 적용하여 구하고 상호 비교함으로써 해석에서 고려한 조건들이 말뚝의 허용횡방향지지력 결정법에 미치는 영향을 알아보고자 하였다. 연구결과에 따르면 말뚝머리 구속조건 및 말뚝길이에 상관없이 연약 점성토 지반에 설치된 말뚝의 경우 지반반력법에 의한 허용횡방향지지력이 설계를 지배함을 알 수 있었고 점성토의 비배수강도가 커짐에 따라 극한횡방향지지력을 통한 허용횡방향지지력이 설계를 지배함을 알 수 있다. 사질토 지반에 설치된 말뚝의 경우 느슨한 사질토지반에 설치된 말뚝머리 자유인 짧은말뚝의 경우를 제외하고 모든 경우에 있어 극한횡방향지지력을 통한 허용횡방향지지력이 설계를 지배함을 알 수 있다. 횡방향말뚝의 설게시 허용 횡방향변위량에 근거한 설계만으로 충분하다는 견해도 있지만 본 연구결과에 따르면 극한횡방향지지력에 근거한 허용횡방향지지력의 계산 또한 필요함을 알 수 있었다. 수평지반반력계수는 허용횡방향변위량 이내에서는 말뚝폭에 큰 영향을 받지 않음을 알 수 있었으며 실제 많이 쓰이는 말뚝폭의 범위인 20~90cm 인 경우 말뚝폭의 영향을 무시해도 될 것으로 생각된다.

• 한국유체기계학회 논문집
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• 제19권3호
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• pp.43-47
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• 2016

Pile foundations constructed on extremely cold regions cause serviceability problems of superstructures from repeated actions of ground freezing and thawing. Oil sand module plants are mainly constructed on seasonal frozen ground. Due to the freezing and thawing actions of grounds, vertical movements of piles have been observed. To solve these erratic pile movement problems, thin vertical layer of PET aggregates is installed around the pile shaft to prevent potential unfavorable pile movements. There is no known method to calculate "thin PET aggregate layer" -surrounded pile shaft resistance (capacity) against vertical loads; therefore, this experimental research is conducted. Specifically, in this study, horizontal (normal) pressures on pile shaft were assessed varying PET aggregate layer thickness based on the experiment.