• Journal of Life Science
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• v.27 no.1
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• pp.67-71
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• 2017

Foulbrood disease is infected by Paenibacillus larvae on larval stage of honeybee, and is lethal disease to result in population death. This disease was manifested in 2008 in Korea, is still suffered by the secondary damages. In this study, we are to examine diagnostic PCR approaches to manage the Foulbrood disease. PCR amplification of 16S rRNA is generally using for microbial infection, but the specificity is little poor for the correct diagnosis. Therefore, we are to identify specific genes expressed in Paenibacillus larvae, and perform PCR analysis. We selected five distinct genes from literature references. Those genes are commonly known as toxic genes for host infection, and include Toxin1, Toxin2A & 2B, SplA, CBP49, and SevA&SevB. PCR amplification for these genes is difficult to detect at the first time. So, we performed the second PCR using the first PCR product as a template. This approach using the nested PCR was very useful for detecting large marker genes. When Paenibacillus larvae was cultured in the medium containing plant extracts, PCR amplification of the identified genes is correlated with the microbial growth inhibition. Therefore, these results suggest that the identified genes might be useful to study diagnostic PCR markers for honeybee Foulbrood disease.

• Journal of fish pathology
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• v.24 no.2
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• pp.113-120
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• 2011

In this study, we examined antimicrobial activity of the bee venom isolated from honeybee (Apis mellifera L.) against fish pathogenic bacteria, Edwardsiella tarda, Vibrio ichthyoenteri and Streptococcus iniae of cultured olive flounder, Paralichthys olvaceus. The bee venom exhibited significant antimicrobial activity against the both Gram-negative bacteria, E. tarda and V. ichthyoenteri and Gram-positive bacteria, S. iniae. Minimum inhibitory concentration (MIC) and minimun bactericidal concentration (MBC) of the bee venom were 17.6 ${\mu}g$/ml 34.6 ${\mu}g$/ml against E. tarda., and 1.76 ${\mu}g$/ml, 6.8 ${\mu}g$/ml against V. ichthyoenteri, respectively. MIC and MBC of the bee venom were 3.49 ${\mu}g$/ml, 11 ${\mu}g$/ml, respectively against S. iniae. The postantibiotic effect (PAE) of the bee venom was 5 hr, 6 hr, and 7 hr against E. tarda, V ichthyoenteri, and S. iniae, respectively. In addition, its antimicrobial activity was stable under various pH conditions. According to these results, the bee venom showed the excellent antimicrobial activity against the tested pathogenic bacteria.

• Mycobiology
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• v.39 no.4
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• pp.317-320
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• 2011

Honey production from approximately 1.6 million colonies owned by about 199,000 Korean beekeepers was almost 23,000 metric tons in 2009. Nosema causes significant losses in honey production and the virus decreases population size. We initiated a survey of honey bee colonies on the blooming period of Acacia to determine the prevalence of Nosema and virus in 2011. Most Korean beekeepers have moved from the south to north of Korea to get Acacia nectar for 2 mon. This provided a valuable opportunity to sample bees originating from diverse areas in one location. Twenty hives owned by 18 beekeepers were sampled in this year. Nosema spore counts ranged from zero to 1,710,000 spores per bee. The average number of nosema spores per bee was 580,000. Approximately 95% of the colonies were infected with Nosema, based on the presence of spores in the flowering period of Acacia. This indicates that Nosema is the predominant species affecting honeybee colonies. Also, the seven most important honeybee viruses were investigated by reverse transcription-PCR. Among them, four different viruses were detected in samples. Black queen cell virus was present in all samples. Chronic bee paralysis virus was detected in 10% of samples. Deformed wing virus was present in only 5% of the samples. Prevalence of Sacbrood virus was 15%. However, Cloudy wing virus, Israel acute paralysis virus and kashmir bee virus were not detected in any of samples.

• Proceedings of the Korean Society of Life Science Conference
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• 2005.09a
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• pp.112-112
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• 2005

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.451-452
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• 2008

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.209.3-209
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• 2005

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1997.05a
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• pp.80-86
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• 1997

꿀벌 및 봉산물 수입에 대응하여 국내 양봉산업의 안정적 발전을 도모하기 위해서는 현실적으로 실내 월동기술의 확립과 봉군월동관리기술의 생력화가 필요하다. 지역의 기후조건 및 봉군관리 실정에 맞는 효율적인 월동기술을 확립하므로서 월동기간중 봉군의 폐사율을 현재 추정되는 20% 수준에서 10%이하가 되도록 줄이고 월동능력을 향상시킬 필요가 있다. (중략)

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.133.1-133
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• 2000

No Abstract, See Full Text

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.69-69
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• 2003

We took the nationwide survey of three main honeybee diseases, European foulbrood (EFB; Melissococcus pluton), chalk brood (CB; Ascosphera apis), and nosema (Nosema spp.) in 2001 and 2002 from South Korea. The number of infected apiaries with EFB and CB examined from 21 apiaries were 9 and 13, respectively. The average percentages of infectedcolonies in apiaries where EFB and CB occurred were 7.4% and 12.8%. (omitted)

• Korean journal of applied entomology
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• v.51 no.3
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• pp.299-303
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• 2012

This study aims to investigate whether geographical variation affects the antibacterial component properties of honeybee (Apis mellifera L.) venom in Korea. Honeybee venom samples were collected from May to September, during 2010 and 2011, from 35 different sites, and were analyzed for major components, including melittin, apamin and phospholipase A2 were determined by a liquid chromatography using ammonium formate, acetonitrile, trifluoracetic acid. On average, melittin, apamin and phospholipase A2 were determined $55.2{\pm}2.07%$, $22.57{\pm}0.103%$, and $12.51{\pm}0.37%$, respectively. The ratio of the major components, including melittin, apamin and phospholipase A2 did not differ significantly according to flower or temperature during collections (One way-ANOVA, Duncan's test (${\alpha}$=0.05)).