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http://dx.doi.org/10.5656/KSAE.2012.06.0.036

Components According to Different Collecting Time and Location in Bee Venom  

Han, Sang-Mi (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Yoon, Hyung-Joo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Baek, Ha-Ju (Gyeongsang Buk-Do Government Public Institute of Health and Environmental)
Publication Information
Korean journal of applied entomology / v.51, no.3, 2012 , pp. 299-303 More about this Journal
Abstract
This study aims to investigate whether geographical variation affects the antibacterial component properties of honeybee (Apis mellifera L.) venom in Korea. Honeybee venom samples were collected from May to September, during 2010 and 2011, from 35 different sites, and were analyzed for major components, including melittin, apamin and phospholipase A2 were determined by a liquid chromatography using ammonium formate, acetonitrile, trifluoracetic acid. On average, melittin, apamin and phospholipase A2 were determined $55.2{\pm}2.07%$, $22.57{\pm}0.103%$, and $12.51{\pm}0.37%$, respectively. The ratio of the major components, including melittin, apamin and phospholipase A2 did not differ significantly according to flower or temperature during collections (One way-ANOVA, Duncan's test (${\alpha}$=0.05)).
Keywords
Bee venom; Collecting time; Collecting location; Melittin; Apamin; Phospholipase A2;
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