• 제목/요약/키워드: Homogenate

검색결과 353건 처리시간 0.03초

마늘장아찌 숙성 중 매운맛과 Allicin량과의 상관관계 (Correlation between Pungency and Allicin Content of Pickled Garlic during Aging)

  • Kim, Mee-Ree;Yun, Jun-Hwa;Sok, Dai-Eun
    • 한국식품영양과학회지
    • /
    • 제23권5호
    • /
    • pp.805-810
    • /
    • 1994
  • Relationship between pungency and allicin content of pickled garlic during aging was examined . Degree of pungency of pickled garlic during aging at 20 $^{\circ}C$ was determined by the sensory evaluation. A panel of 10 members evaluated seven samples of pickled garlic which were aged for 0, 10 , 20, 40 , 50 or 60 days by using scoring test (seven point scale). The sensory evaluation results showed that pungency of pickled garlic decreased gradually during aging, and scored at 3.07 on the 40 th day of aging. Content of allicin, which was a major pungent component of garlic homogenate, was quantitatively analyzed by HPLC. The level of allicin in homogenate of pickled garlic was found to decrease gradually, and to 5.9% on the 40 th day of aging compared with that of fresh garlic. Relationship between the pungency score results and the content of allicin demonstrated a highly positive correlation (r=0.9648).

  • PDF

피하조직에 투여된 수은과 카드뮴의 효소활성과 과산화지질에 미치는 영향 (The Effects of Mercury and Cadmium Administered in Subcutaneous Tissue on Enzymatic Activity and lipidperoxidation)

  • 하배진
    • 한국환경과학회지
    • /
    • 제11권6호
    • /
    • pp.583-588
    • /
    • 2002
  • Heavy metals like Mercury and Cadmium cause various kinds of toxicities in the organs of Liver and Kidney. To observe the results of toxicity in the liver, kidney, and serum when the rats were injected subcutaneously with HgCl$_2$ and CdC1$_2$ and sacrificed after 24 hours and 72 hours from the last injection, we measured variation of lipidperoxide values in rat liver homogenate, variation of aspartate aminotransferase and alanine aminotransferase in rat serum. Variation of lipidperoxide values in rat kidney homogenate and variation of BUN in rat serum. It was found that Mercury and Cadmium administered subcutaneously to the skin in the air could cause the damages of liver and kidney.

The Bioactivity of Natural Product in the Ovariectomized Rat

  • Ha, Bae-Jin
    • Journal of Life Science
    • /
    • 제11권1호
    • /
    • pp.47-51
    • /
    • 2001
  • To investigate the deaging effects of introperitoneally injected Chondroitin Sulfate (CS) on various enzyme activity (AST, ALT, MDA (Malon dialdehyde), SOD (Superoxide dismutase), GPx (Glutathione peroxidases) and histophathology of liver tissue, ovariectomized rats were used. The antioxidative effects of chondroitin sulfate (100 mg/kg and 200 mg/kg body weight) were investigated at the antioxidative enzyme activities of liver homogenate fractions (liver total homogenate, mitochondrial, and microsomal fractions) and sera. In addition, the rat liver was histologically examined. Intraperitoneally injected CS, depend on dosage, indicated a protective effect against ovariectomy-inducted aging. Moreover, inflammation and cirrhosis in liver tissue of CS treated group were significantly decreased. Based on these results, intraperitoneally injected CS is a useful material to delay aging.

  • PDF

알콜유도 지질과산화에 미치는 어성초 영향 (Effect of Houttuynia cordata on EtOH-induced Lipidperoxidation in Rat)

  • 강탁림
    • 대한예방한의학회지
    • /
    • 제2권1호
    • /
    • pp.45-54
    • /
    • 1998
  • The antilipidperoxidative and hepatopreventive effects of Houttuynia cordata extract(HCE) were investigated at the level of liver homogenates and serum of SD rats intoxicated EtOH(3g/kg for 3days). We measured MDA(malondialdehyde) in the live. homogenate, AST(1-aspartate-2-oxoglutarate aminotransferase) and ALT( 1-alanine-2-oxoglutarate aminotransferse) in the serum. The analysis of the measurement indicated that HCE reduced MDA, ALT and AST significantly and their reduction was in relation to dose dependence. In rat liver homogenate intoxicated with EtOH, HCE treatment group inhibited lipidperoxidation by 50-75%. In rat serum intoxicated EtOH, HCE inhibited AST and ALT by 40-70%.

  • PDF

중금속의 생체독성에 대한 알로에의 저감 연구 (A Study on the Reduction of Heavy Metal Biotoxicity by Aloe)

  • 하배진
    • 한국환경과학회지
    • /
    • 제7권1호
    • /
    • pp.46-51
    • /
    • 1998
  • The effects of aloe on the MDA(malondialdehyde) and the blood biochemical components of heavy metal poisoning in SD rat were examined and the following results were obtained. In rat liver homogenate intoxicated with $CdCl_2$, lipidperoxide was increased each 2.37times(24h), 3.31times(72h) but lipidperoxide In aloe administration groups was lower each 47% , 64% than in heavy metal group. In rat kidney homo- genate intoxicated with $CdCl_2$, lipidperoxide was increased 1.85times(24h), 1.33times(72h) but lipidperoxide in groups was almost the same as that of normal group. Lipidperoxide of kidney homogenate was slightly decreased as time passed. Also heavy metal poisoning rats showed high levels(1.38-2.50times) of serum AST, ALT and BUN. However. the administration of aloe significantly inhibited the reduction of them. These results suggest that Cd-induced hepatic and renal injury, via increase llpidpero)Ode and release of AST, ALT and BUN. Aloe may be used to inhibit or prevent the hepatic and renal toxicity which results from the heavy metal.

  • PDF

갓김치 Chlorophylls 및 Carotenoids의 항산화 효과 (Antioxidative Effect of Cholorophylls and Carotenoids in Mustard Leaf Kimchi Activity)

  • 송은승;전영수;최홍식
    • 한국식품영양과학회지
    • /
    • 제30권3호
    • /
    • pp.421-425
    • /
    • 2001
  • Antioxidative effects of crude chlorophylls and carotenoids extracts from mustard leaf kimchi on the lipid peroxidation in rat liver homogenate, egg phosphatidyl choline (EPC) liposome and superoxide anion radical were examined. The extracts were found to inhibit the increase of the thiobarbituric acid (TBA) value and show the effect of antioxidative activity on the liposomal phospholipid membrane. The oxidation index of EPC liposome was markedly decreased in the prescence of the extracts. The antioxidative activity of the extracts from mustard leaf kimchi was not related with fermentation period of the kimchi. The extracts from mustard leaf showed the similar antioxidative activity of $\alpha$-tocopherol within in the given level of addition. However, the oxidation index. When the effect of the extracts from mustard leaf kimchi on free radical scavenging was observed by the determination of the superoxide anion radical scavenging activity, it had similar value to that of $\alpha$-tocopherol.

  • PDF

알로에의 과산화지질 억제효과에 관한 연구 (Studies on the Antilipidperoxidative Effect of Aloe)

  • 하배진
    • 한국식품위생안전성학회지
    • /
    • 제11권2호
    • /
    • pp.159-164
    • /
    • 1996
  • The antilipidperoxidative and hepatopreventive effects of Aloe water extract (30 mg, 50 mg, 100 mg) were investigated at the levels of liver-total homogenates and the sera of SDrats intoxicated with CCl4 (0.5 cc/100g) and 50% ethanol. We measured MDA (Malondialdehyde) in the liver homogenate, AST (L-Aspartate-2-oxoglutarate aminotransferase) and ALT(L-Alanine-2-oxo-glutraate aminotransferase) in the serum. The analysis of the measurement indicated that Aloe water extract reduced MDA, ALT and AST significantly and their reduction was in relation to dose dependence. In rat liver homogenate intoxicated with ethanol and CCl4, Aloe treatment group markedly inhibited lipidperoxidation by 30%∼70%. In rat serum intoxicated with ethanol and CCl4, Aloe treatment group inhibited AST, ALT by 40%∼90%. In these data Aloe may be used to inhibit or prevent the hapatic toxicity with results from the environmental and alcohlic factors through the further study of its exact antihepatotoxic mechanism.

  • PDF

저산소/재관류로부터 청폐사간탕의 PC12 세포 보호 효과 (Protective Effect of Metabolized Chungpesagan-tang on Hypoxia/Reperfusion Induced-PC12 Cell Damage)

  • 소윤조
    • 생약학회지
    • /
    • 제36권2호통권141호
    • /
    • pp.151-157
    • /
    • 2005
  • This research was performed to investigate the protective effect of Chungpesagan-tang (CST) from hypoxia/reperfusion induced-PC12 cell damage. To elucidate the mechanism of the protective effect of CST, cell viability, changes in activities of superoxide dismutase, glutathione peroxidase, catalase, caspase 3 and the production of malondialdehyde were observed after treating PC12 cells with CST which was metabolized by rat liver homogenate. Pretreatment of CST with liver homogenate appeared to increase its protective effect against hypoxia/reperfusion insult. The result showed that CST exhibited the highest protective effect against hypoxia/reperfusion at the dose of $1\;{\mu}g/ml$ in PC12 cells, probably by recovering the redox enzyme activities and MDA to control level.

11,12-Dihydroarteannuic acid에서 artemisinin의 생합성 (Biosynthesis of artemisinin from 11,12-dihydroarteannuic acid)

  • 김남철;김수언
    • Applied Biological Chemistry
    • /
    • 제35권2호
    • /
    • pp.106-109
    • /
    • 1992
  • 국화과의 일증인 개똥쑥(Artemisia annua L., 청호(靑蒿))에는 항말라리아 물질인 artemisinin이 함유되어 있다. 11,12-Dihydroarteanuic acid는 cell-free 방법으로 수행한 실험에서, Agrobacterium tumefaciens로 형질 전환된 crown gall의 homogenate와의 incubation에서 artemisinin으로 전환되었다. 그러나 arteannuic acid는 NADPH와 함께 incubation하였을 때 dihydro acid로 전환되지 않았다. 따라서 이 결과는 식물체의 조건에 따라서, dihydro acid가 자발적으로 뿐만 아니라, 생체내에서 효소 반응에 의해서도 artemisinin으로 전환될 수 있음을 나타낸다.

  • PDF

식용 및 약용식물 추출물과 화합물이 흰쥐의 간장조직에서 과산화지질 생성에 미치는 영향 (Effects of the Components and Extracts of Some Edible and Medicinal Plants on the Formation of Lipid Peroxide in Rat Liver Homogenate)

  • 박종철;정신교;이종호;허종문;최명락;송상호;최종원
    • 한국식품영양과학회지
    • /
    • 제26권6호
    • /
    • pp.1159-1163
    • /
    • 1997
  • We have studied the effect of 31 extracts and 10 components from some edible and medicinal plants on the formation of lipid peroxide in the liver homogenate of rat in vitro. The 70% acetone extracts of Allium tuberosum, Beta vulgaris var. cicla and Brassica juncea var. integrifolia, and methanol extract of Capsicum annuum decreased the formation of lipid peroxide by 33%, 58%, 62% and 56% at the concentration of 1mg/ml, respectively. And these four extracts inhibited the lipid peroxidation at the concentration of $10^{-1}$mg/ml by 17%, 46%, 49% and 45% respectively. Among the component tested, quercetin, quercitrin and isorhamnetin reduced the formation of lipid peroxide by 45%, 15% and 28% respectively at the concentration of $10^{-2}$mg/ml.

  • PDF