• 제목/요약/키워드: Homo-Association

검색결과 45건 처리시간 0.024초

cDNA Microarray Analysis of Differential Gene Expression in Boar Testes during the Prepubertal Period

  • Lee, Dong-Mok;Lee, Ki-Ho;Choi, Jin Ho;Hyun, Jin Hee;Lee, Eun Ju;Bajracharya, Prati;Lee, Yong Seok;Chang, Jongsoo;Chung, Chung Soo;Choi, Inho
    • Asian-Australasian Journal of Animal Sciences
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    • 제22권8호
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    • pp.1091-1101
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    • 2009
  • In an attempt to understand the biochemical mechanism for the synthesis of the anabolic steroid, 19-nortestosterone, produced by prepubertal boar testes and its physiological role, normalized complementary DNA (cDNA) from boar testes was generated. DNA sequencing of 2,016 randomly selected clones yielded 794,116 base pairs of high quality nucleotide sequence. Computer-assisted assembly of the nucleotide sequence of each clone resulted in 423 contigs and 403 singletons including several genes for steroidogenic enzymes and molecules related to steroid metabolism. Analysis of gene expression pattern by use of the presently-fabricated cDNA microarray identified a number of genes that were differentially expressed during the postnatal development period in boar testes. Two genes of unknown function were identified to be highly expressed in the testis of 2-weeks-old neonatal boar. In addition, the sequencing of open reading frames of these genes revealed their homology with human alpha hemoglobin and Homo sapiens hypothetical LOC643669, transcript variant 1. Moreover, the transcripts of these genes were also detected in porcine muscle and adipocytes, in addition to Leydig cells of pigs.

영상기반의 바이브라이미지 기술을 이용한 자극 검사에 대한 연구 (Research of video based Vibraimage technology stimulation examination KOCOSA)

  • 이재석;이일호;이태현;최진관;정석화;한지수
    • 융합보안논문지
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    • 제15권3_1호
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    • pp.41-51
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    • 2015
  • 인간의 속임수는 동물과 차원이 다르다 상대방뿐만 아니라 자신도 속일 수 있다. 심리학 용어로 '자기기만'이라고 한다. 일반적으로 거짓말을 할 때는 티가 난다. 말은 어떻게 짜맞춰보더라도 호흡이나 땀, 시선, 표정, 목소리까지 완벽히 통제하는 데는 한계가 있다. 하지만 자신의 거짓말을 스스로 믿는 다면, 비언어적인 표현조차 거짓을 바꿀 수 있다. 호모사이피엔스가 네안데르탈인을 몰아내고 마침내 지구를 차지할 수 있었던 것은 언어인 거짓말의 능력이라고 해도 과언이 아니다. 그래서 인류를 '호모 팔락스'라고 부르기도 한다. 인간의 이러한 거짓말에 대한 발전 못지않게 거짓말탐지에 대한 능력 또한 비약적으로 증대되었다. 20세기 가장 뛰어난 발명품 중의 하나인 폴리그래프 검사기는 진화를 거듭하여 피검사자나 검사자 모두에게 이용하기 편리한 비접촉식으로 검사를 할 수 있는 바이브라이미지 기술이 출현하게 되었다. 본 논문에서는 이러한 바이브라이미지 기술을 이용한 거짓탐지기법인 영상 계층진술분석기법의 연구개발과정에서 발견된 표준검사인 자극 검사를 소개한다.

Cloning and Sequence Analysis of Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Yak

  • Li, Sheng-Wei;Jiang, Ming-Feng;Liu, Yong-Tao;Yang, Tu-Feng;Wang, Yong;Zhong, Jin-Cheng
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권11호
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    • pp.1673-1679
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    • 2008
  • In order to study the biological function of gapdh gene in yak, and prove whether the gapdh gene was a useful intra-reference gene that can be given an important role in molecular biology research of yak, the cDNA sequence encoding glyceraldehyde-3-phosphate dehydrogenase from yak was cloned by the RT-PCR method using gene specific PCR primers. The sequence results indicated that the cloned cDNA fragment (1,008 bp) contained a 1,002 bp open reading frame, encoding 333 amino acids (AAs) with a molecular mass of 35.753 kDa. The deduced amino acids sequence showed a high level of sequence identity to Bos Taurus (99.70%), Xenopus laevis (94.29%), Homo sapiens (97.01%), Mus musculus (97.90%) and Sus scrofa (98.20%). The expression of yak's gapdh gene in heart, spleen, kidney and brain tissues was also detected; the results showed that the gapdh gene was expressed in all these tissues. Further analysis of yak GAPDH amino acid sequence implied that it contained a complete glyceraldehyde-3-phosphate dehydrogenase active site (ASCTTNCL) which ranged from 148 to 155 amino acid residues. It also contained two conserved domains, a NAD binding domain in its N-terminal and a complete catalytic domain of sugar transport in its C-terminal. The phylogenetic analysis showed that yak and Bos taurus were the closest species. The prediction of secondary structures indicated that GAPDH of yak had a similar secondary structure to other isolated GAPDH. The results of this study suggested that the gapdh gene of yak was similar to other species and could be used as the intra-reference to analyze the expression of other genes in yak.

정보기술문화와 가속화된 미래에 대한 환경 비전 -돈 들릴로의 『코스모폴리스』 (The Environmental Vision in Information Technology Culture and Accelerated Future: Don DeLillo's Cosmopolis)

  • 이정희
    • 영어영문학
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    • 제58권5호
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    • pp.943-974
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    • 2012
  • This paper aims to suggest the compromising vision of nature and technology as the solution to get out of the globally accelerated technology environment in Don DeLillo's Cosmopolis. This novel intends to emphasize on the importance of physical environment as a precondition for the survival of human. Eric wants to be a posthuman with the cybernetic idea, pursuing to be the digital self in a vast biosphere that integrates both the nature and the technology. His obsessive worship of technology through his quest for the futurity results in the effacement of the humanity and the insulation from the nature. Cosmopolis is DeLillo's first 9/11 novel, which describes a young-billionaire asset manager Eric's one-day life in New York in April 2000. Eric can be the third Twin Tower as a symbol of global economic hegemony. By the allusion of the 9/11 catastrophic event, it can be said that Eric's fall is caused by his hubris and avarice as a global capitalist. Crossing the 47th Street toward the West in his limousine, his journey is revealed as the environmental reflections on his desires to attain the futurity and transcendence by technology. This novel cautions that the abuse of technology can bring out the obsolescence and erasure of the humanity and the nature. DeLillo suggests that the best hope for the evolutionary possibility of posthuman can be realized through the correlation with nature and technology. This future-oriented novel warns that the excessive technology should not lead to the disappearance of community and humanity, and the separation of self and nature. It admonishes that they should not follow pseudo-cosmopolitanism as the greedy world citizens, devoting on the velocity of newest technology. This novel recommends that humans should be the world citizen of global ecosystem, making the ameliorative environment through the correlation with self/environment and technology/nature, and gardening the restorative biosphere and the younger planet.

사용자 욕구조사를 통한 해양레저용품(Bodyboard)디자인 컨셉개발 (Case Study on Bodyboard Design Development though User's needs)

  • 강범규
    • 한국콘텐츠학회논문지
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    • 제13권5호
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    • pp.135-145
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    • 2013
  • 인간의 특징을 설명하는 단어 중 하나가 "유희적 인간"이다. 또한 최근 생활수준이 높아지고 삶이 더욱 윤택해짐에 따라 놀이는 인간 삶의 중심 가치로 부상하고 있다. 최근 해양레저산업의 규모가 확대됨에 따라 해양용품 개발의 필요성이 대두되고 있으며, 해양레저장비를 수입에 의존해 오던 국내 시장에서 국산 해양 레저 용품의 개발이 필요성을 더욱 커지고 있다. 본 연구는 바디보드(bodyboard)의 신제품 개발에 있어서 제품분석 및 소비자 욕구 조사를 통한 국내 시장에 적합한 새로운 바디보드의 디자인 컨셉 제안을 목표로 한다. 연구 결과는 다음과 같다. 첫째, 한국시장의 바디보드는 14세에서 29세 사이의 젊은 층을 위한 40,000원~100,000원 대의 가격 포지셔닝이 바람직하다. 둘째, 작은 파도 위주의 국내 해안환경에도 튜브형 바디보드가 적합하다. 튜브형 바디보드의 선호고객이 65%이상으로 조사되었다. 셋째, 새로운 가치 창출형 바디보드 개발을 위하여 본 연구에서는 새로운 CMFF(Color, Material, Form, Function)전략이 개발되었다. 본 연구결과는 객관적이고 과학적인 소비자 조사가 이루어지지 않았던 바디보드개발의 연구 분야에 있어서 새로운 제품 디자인개발 방법을 제시했다는 점에서 의미를 가진다.

원발성 및 전이성 구강편평세포암종 세포주에서 p21 및 p73 mRNA발현에 관한 연구 (STUDY ON mRNA EXPRESSION OF P21 AND P73 IN THE CELL LINES OF PRIMARY AND METASTATIC SQUAMOUS CELL CARCINOMA)

  • 강정훈;김경욱;이재훈
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제27권6호
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    • pp.483-490
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    • 2001
  • There were many controversies in the cause and progress of tumorigenesis. Recently, studies on the mutation of genes related to the tumor have extensively been performed due to development of molecular biology. Structural and morphological changes of chromosomes, which are related to the abnormal activation of oncogenes or inactivation of tumor suppression genes, transform the normal cells into the tumor cells. p53 and Rb are well known tumor suppressor genes, while oncogenes include c-myc, bcl-2 and ras, etc. When exposed to cell damaging agents, p53 inhibits cell growth by inducing transcription of p21. Especially p73, which is homo-logy of p53, frequently deleted in melanoma, neuroblastoma, colon cancer, and breast cancer, when over produced, p73 activates the transcription of p21, bax-1 and inhibits cell growth by inducing apoptosis. For study on mRNA expression of p21 and p73, normal oral keratinocytes, and cell lines of primary and metastatic oral squamous cell carcinoma were cultured and then electrophoresis and RT-PCR(reverse transcription-polymerase chain reaction) were performed. 1. The mRNA of p21 and p73 in normal oral keratinocyte expressed lower than that of primary squamous cell carcinoma. 2. The mRNA of p21 in metastatic oral squamous carcinoma cell lines was expressed as various patterns compared with that of normal oral keratinocyte. 3. In the metastatic oral squamous cell lines, the mRNA of HN8 expressed higher than that of HN12 or HN19. 4. The mRNA of p73 in primary oral squamous cell lines expressed 4-5 times higher than that of normal keratinocyte. 5. In metastatic oral squamous cell lines, there was no significant expression of p73 mRNA compared with that of normal oral keratinocyte. From the results obtained in this study, mRNA expression of p73 in primary oral squamous cell lines was remarkable, while mRNA expression of p21 and p73 in metastatic oral squamous cell lines were statistically insignificant.

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가정과 주생활 교육을 위한 코하우징 교수.학습 과정안 개발 및 평가 (Development and Evaluation of the Cohousing Teaching.Learning plan)

  • 김미향;조재순
    • 한국가정과교육학회지
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    • 제23권2호
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    • pp.55-69
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    • 2011
  • 이 연구에서는 중 고등학교에 적용할 수 있는 코하우징 교수 학습 과정안을 개발하고 평가하여 그 내용과 방법의 적절성을 알아보는 것을 목적으로 하였다. 이 과정안은 자료수집 및 분석 설계, 개발, 실행, 평가 5단계를 거쳐 개발되었다. 자료수집 및 분석단계에서는 스웨덴 코하우징 단지 답사, 코하우징 국제 컨퍼런스 참석, 선행연구 분석을 하였으며, 설계 단계에서는 교수 학습 내용 선정과 배치, 수업 실행과 참관에 대한 계획 및 평가문항 개발계획을 세웠다. 개발 단계에서는 교수 학습 과정안 초안을 개발하여 교육 전문가 집단토의 2회, 코하우징 전문가, 수업 지도 교사와의 협의를 통해 수정, 보완하여 최종안을 완성하였다. 실행 단계에서는 중학교 2개교의 3학년과 고등학교 1개교 1학년 학생을 대상으로 수업이 이루어졌다. 평가 단계에서는 학생과 지도교사의 코하우징 수업에 대해 설문조사한 결과를 분석하였다. 개발된 코하우징 교수 학습 과정안은 1차시에는 코하우징의 정의와 필요성, 2차시에는 코하우징의 공간구성, 3차시에는 코하우징 마을 만들기를 주제로 학습활동 하도록 구성하였다. 관련 학습자료로는 개인 활동지(2개), 모둠 활동지(2개), 개인 활동 교구(2세트), 모둠 활동 교구(3세트), 텍스트 자료(8개), 파워포인트(3편)를 개발하였다. 수업은 경기도소재 중고교 가정과 교사 3인이 2010. 8. 23~9. 17까지 3개교 28개반(총1125명) 학생을 대상으로 실행하였으며 연구자가 일부 수업을 참관하였다. 학생들의 평가에서는 수업 과정, 코하우징에 대한 이해도, 수업에 대한 흥미도 만족도가 모두 높았으며, 중학교와 고등학교간 유의미한 차이가 없어 과정안이 개발된 코하우징 교수 학습 과정안이 중학교와 고등학교에서 모두 적용 가능함을 시사하고 있다. 교사들도 학습자료와 수업내용을 매우 높게 평가하였으며, 연구자는 수업 참관을 통해 이 교수 학습 과정안이 학교사정에 따라 응용이 가능함을 관찰할 수 있었다.

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디지털 창의노동 -젊은 세대의 노동 윤리와 주체성에 관한 한 시각 (Digital Creative Labour -A Perspective of the Ethics of Labour and Subjectivity of the Younger Generation in Korea)

  • 김예란
    • 한국언론정보학보
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    • 제69권
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    • pp.71-110
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    • 2015
  • 디지털 창의 노동이란 문화화와 소비주의가 확장하며 노동질서의 유연성과 불안정성이 격화하고 신자유주의적인 이념이 지배적인 자본주의 사회에서, 자유 자율 자발을 추구하는 에토스와 문화적 창의성과 참여를 지향하는 실천들이 디지털 네트워크를 매개로 왕성하게 촉발, 발현, 교통되고, 이들이 정치 경제 문화 권력과 다양한 방식들로 접합 혹은 분절하는 노동 문화를 의미한다. 디지털 창의노동은 개인의 자유를 기치로 표방하는 신자유주의적인 이념 체제 안에 있으면서도 기업화된 디지털 자본주의의 자장 안에서 생성되기에 통치권력과 주체형성의 모순과 갈등으로부터 결코 자유로울 수 없다. 창의성과 열정, 자기 혁신과 성공적인 미래와 같은 긍정적 가치가 강조되는 동시에 이것을 실행하는 경제적 합리성과 자기 경영의 원리가 중시된다. 그 과정에 주체의 의지, 기업의 경영기술, 대중의 익명화된 권력, 사회적으로 팽배한 상상과 환영의 힘들이 개입하면서 자기 통치적인 주체가 형성된다. 이처럼 디지털 창의노동자는 창의성과 자유의 문화적 가치를 지향하고 디지털 네트워크의 참여성과 자발성의 가치를 추구하기에 오히려 신자유주의적인 경제 체제에 타협적으로 접합되는 역설 안에 있다.

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Cloning and sequence analysis of Wild Argali short palate, lung and nasal epithelium clone 1 cDNA

  • Shen, Wen;Chen, Kaili;Sun, Yanming;Guo, Haiying;Chen, Dongmei;Cao, Yang
    • Asian-Australasian Journal of Animal Sciences
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    • 제30권5호
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    • pp.736-742
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    • 2017
  • Objective: Experiments were conducted to clone the sequence of Wild Argali short palate, lung and nasal epithelium clone 1 (SPLUNC1) cDNA, and to lay the foundation for further study the biological function of Wild Argali SPLUNC1. Methods: The complete sequence of Wild Argali SPLUNC1 cDNA was generated by rapid amplification of cDNA ends. The entire coding sequence was inserted into the pPIC9K vector and expressed in Pichia pastoris (P. pastoris) GS115. The recombinant SPLUNC1 protein was detected by Western blot and purified by $Ni^{2+}$ chelate affinity chromatography. The test of effect of the protein on Mycoplasma ovipneumoniae (MO) was performed with real-time polymerase chain reaction. Results: The Wild Argali SPLUNC1 cDNA was 1,076 bp with an open reading frame of 768 bp, which encoded a 26.49 kDa protein composed of 255 amino acids. Its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8%, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of SPLUNC1 cDNA from Ovis aries (accession no. NP_001288334.1), Capra hircus (accession no. XP_005688516.1), Pantholops hodgsonii (accession no. XP_005979709.1), Bos taurus (accession no. NP_776851.1), Felis catus (accession no. XP_006929910.1), Homo sapiens (accession no. NP_001230122.1), Sus scrofa (accession no. NP_001005727.1), Chinchilla lanigera (accession no. NP_001269294.1), Mus musculus (accession no. NP_035256.2), and Rattus norvegicus (accession no. NP_742028.1), respectively. The recombinant protein corresponded to the expected molecular mass of 25.47 kDa as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was detected in the supernatant of P. pastoris, and it could be purified. The results from the test of inhibition effect of argali recombinant SPLUNC1 protein on MO showed that the product could inhibit MO very well (p<0.01). Conclusion: The amino acid sequence of Wild Argali SPLUNC1 was different from other organisms. The recombinant SPLUNC1 protein has good biological activity.

Nutrient Digestibility, Ruminal Fermentation Activities, Serum Parameters and Milk Production and Composition of Lactating Goats Fed Diets Containing Rice Straw Treated with Pleurotus ostreatus

  • Kholif, A.E.;Khattab, H.M.;El-Shewy, A.A.;Salem, A.Z.M.;Kholif, A.M.;El-Sayed, M.M.;Gado, H.M.;Mariezcurrena, M.D.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권3호
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    • pp.357-364
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    • 2014
  • The study evaluated replacement of Egyptian berseem clover (BC, Trifolium alexandrinum) with spent rice straw (SRS) of Pleurotus ostreatus basidiomycete in diets of lactating Baladi goats. Nine lactating homo-parity Baladi goats (average BW $23.8{\pm}0.4$ kg) at 7 d postpartum were used in a triplicate $3{\times}3$ Latin square design with 30 d experimental periods. Goats were fed a basal diet containing 0 (Control), 0.25 (SRS25) and 0.45 (SRS45) (w/w, DM basis) of SRS. The Control diet was berseem clover and concentrate mixture (1:1 DM basis). The SRS45 had lowered total feed intake and forages intake compared to Control. The SRS25 and SRS45 rations had the highest digestibilities of DM (p = 0.0241) and hemicellulose (p = 0.0021) compared to Control which had higher (p<0.01) digestibilities of OM (p = 0.0002) and CP (p = 0.0005) than SRS25 and SRS45. Ruminal pH and microbial protein synthesis were higher (p<0.0001) for SRS25 and SRS45 than Control, which also had the highest (p<0.0001) concentration of TVFA, total proteins, non-protein N, and ammonia-N. All values of serum constituents were within normal ranges. The Control ration had higher serum globulin (p = 0.0148), creatinine (p = 0.0150), glucose (p = 0.0002) and cholesterol (p = 0.0016). Both Control and SRS25 groups had the highest (p<0.05) milk (p = 0.0330) and energy corrected milk (p = 0.0290) yields. Fat content was higher (p = 0.0373) with SRS45 and SRS25 groups compared with Control. Replacement of BC with SRS in goat rations increased milk levels of conjugated linoleic acid and unsaturated fatty acids compared with Control. It was concluded that replacing 50% of Egyptian berseem clover with SRS in goat rations improved their productive performance without marked effects on metabolic indicators health.