• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.4
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• pp.360-365
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• 2009

The dietary fiber and crude fiber contents of different thallus regions (blade, stipe, and holdfast) in three brown algae (Laminaria japonica, Ecklonia stolonifera, and E. cava) were detennined at different ages, and then compared with one another. On a dry matter basis, the soluble dietary fiber (SDF) content was highest ($10.8{\pm}0.5%$) in the holdfast of 2-year old L. japonica, and the insoluble dietary fiber (IDF), total dietary fiber (TDF), and crude fiber (CF) contents were highest in the holdfast of 2-year old E. cava at $44.5{\pm}0.7%$, $50.2{\pm}0.5%$, $6.8{\pm}0.7%$, respectively. The IDF, TDF, and CF contents of these three species were measured in the following order: holdfast > stipe > blade, and the SDF contents of L. japonica exhibited the reverse of this trend. The TDF/CF ratio of 1-year old L. japonica, E. stolonifera, and E. cava was greater than was observed in the corresponding 2-year old samples. This is, to the best of our knowledge, the first report demonstrating that the holdfasts of L. japonica, E. stolonifera, and E. cava are rich in dietary fiber contents, especially IDF, TDF, and CF.

• Ocean and Polar Research
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• v.42 no.2
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• pp.133-139
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• 2020

The aim of this study is to examine the physiological characteristics of an agarophyte Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris et Fredericq in the early life stage of tetrasporophytes (2n) and gametophytes (n) to select appropriate seedlings for mariculture. Growth experiments were carried out at the combinations of four temperatures (20, 25, 30, and 35℃) and three light intensity levels (20, 60, and 100 µmol photons m^-2 s^-1) in the two ontogenetic stages: discoid holdfasts and erect sporelings. Holdfast areas and sporeling lengths of tetrasporophytes and gametophytes were estimated after 14 days in culture. Relative growth rates (RGRs) for holdfast areas were 7.08-28.38% day^-1 for tetrasporophytes and 11.58-23.67% day^-1 for gametophytes. At 35℃, holdfasts of tetrasporophytes survived with RGRs of 7.08-23.28% day^-1 but those of gametophytes died. Maximal holdfast growth of tetrasporophytes occurred at 30℃ and 100 µmol photons m^-2 s^-1, which were different from gametophytes (25℃ and 100 µmol photons m^-2 s^-1). RGRs of tetrasporophytic sporelings were 2.93-11.11% day-1 and were between 0.78-10.82% day-1 for gametophytes. Maximal growth of A. vermiculophyllum sporelings occurred at 25℃ and 60 µmol photons m^-2 s^-1 for tetrasporophytes, and at 20℃ and 100 µmol photons m^-2 s^-1 for gametophytes. In conclusion, the present results indicate that carpospores could be used as resources of spore-seedling methods having genetic diversity for mass field cultivation because tetrasporophytes showed higher-temperature tolerance and faster-growing ability than gametophytes of A. vermiculophyllum in the discoid holdfast and sporeling stages.

• ALGAE
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• v.25 no.3
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• pp.141-153
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• 2010

Seagrass epiphyte blooms potentially have important economic and ecological consequences in Tampa Bay, one of the Gulf of Mexico's largest estuaries. As part of a Tampa Bay pilot study to monitor the impact of environmental stresses, precise characterization of epiphyte diversity is required for efficient management of affected resources. Thus, epiphyte diversity may be used as a rational basis for assessment of ecosystem health. In May 2001, epiphytic species encompassing green, brown and red macroalgae were manually collected from dense and sparse seagrass beds of Thalassia testudinum and Syringodium filiforme. A total of 20 macroalgal epiphytes, 2 Chlorophyta, 2 Phaeophyta, and 16 Rhodophyta, were found on T. testudinum and S. filiforme seagrass at the four sampling sites (Bishop Harbor, Cockroach Bay, Feather Sound, and Mariposa Key). The Rhodophyta, represented by 16 species, dominated the numbers of species. Among them, the thin-crusted Hydrolithon farinosum was the most commonly found epiphyte on seagrass leaves. Species number, as well as species frequency of epiphytes, is higher at dense seagrass sites than sparse seagrass sites. Four attachment patterns of epiphytes can be classified according to cortex and rhizoid development: 1) creeping, 2) erect, 3) creeping & erect, and 4) erect & holding. The creeping type is characterized by an encrusting thallus without a rhizoid or holdfast base. Characteristics of the erect type include a filamentous thallus with or without a cortex, and a rhizoid or holdfast base. The creeping and erect type is characterized by a filamentous thallus with a cortex and rhizoid. A filamentous thallus with a cortex, holdfast base, and host holding branch is characteristics of the erect and holdfast attachment type. This study characterized each species found on the seagrass for epiphyte identification.

• Parasites, Hosts and Diseases
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• v.46 no.2
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• pp.83-86
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• 2008

Holostephanus metorchis (Digenea: Cyathocotylidae) is a parasite of birds, transmitted by freshwater fishes. H. metorchis adults were recovered from chicks experimentally infected with metacercariae collected from freshwater fishes, Pseudorasbora parva. The metacercariae were oval, surrounded with thick fibrous capsules. In adult flukes, the holdfast organ occupied the ventral concavity, and the anterior testis did not reach the level of the ventral sucker. Based on these morphological characteristics, these flukes were identified as H. metorchis.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.618-624
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• 2010

This study was conducted to establish the in vitro seed germination of Loranthus tanakae. A factorial experiment evaluated the effects of seed storage duration (0, 8, 16 weeks), media (MS, SH, White, WPM), presence of viscin, GA3, gelling agent and concentrations. Seed germinated after one week culture in in vitro condition and produced radicles. In vitro seed germination was optimal when the seeds removed viscin placed on SH medium (69%) and the addition of 0.35% gelrite (75%) in the same medium. As seed storage duration was expanded to 8 or 16 weeks, in vitro seed germination rate was reduced rapidly. Holdfasts were also produced at the side of radicles. The important factors to produce holdfast and haustorium was kind of media as an optimal condition in White medium without any supplements to be shown 98% and 8% respectively. Process of in vitro germination of Loranthus tanakae was followed to radicle elongation, holdfast development and then haustorium formation sequencially.

• ALGAE
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• v.20 no.3
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• pp.177-181
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• 2005

Two species of marine algae, Fauchea spinulosa Okamura et Segawa (Rhodymeniaceae, Rhodophyceae) and Stictyosiphon soriferus (Reinke) Rosenvinge (Chodariaceae, Phaeophyceae) were newly collected from the southern coast and Cheju Island of Korea. Fauchea spinulosa was collected from subtidal zone in the insular region of the southern coast. Plants were erect from the discoidal holdfast with short stipe, pinkish to deep red, cartilageneous, dichotomously and flabellately branched, and 10-15 cm high, 5-15 mm broad. Tetrasporangia are cruciately divided and nemathecia occurred on a side of the branches. Cystocarps were mostly coronate in marginal area and spermatangia were scattered on both sides of branches. Stictyosiphon soriferus commonly occurs on muddy and sandy intertidal flat of Namhaedo located on the southern coast. Plants are epilithic, light brown, terete, 3-5 cm high, and branched heavily in irregular or alternate manner, arising from a small holdfast with rhizoidal clumps. Plurilocular sporangia were scattered in patches and slightly swollen above the cortex of the whole filament. Unilocular sporangia were not found.

• ALGAE
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• v.37 no.1
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• pp.15-32
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• 2022

Inspecting herbaria collections of Laurencia rigida highlighted frequent misidentifications between L. rigida and L. heteroclada f. decussata, two poorly studied taxa from Australia. Recent collections of DNA material, including from topotype material, allowed for re-examination of these two taxa using molecular techniques. Detailed morphological and molecular analyses based on two markers (rbcL and COI-5P) strongly supported these two taxa as being distinct from each other and requiring nomenclatural changes. Comprehensive morphological analyses highlighted features useful for accurate identifications. Interestingly, L. rigida was found to belong to the genus Palisada with evidence from both the morphology and molecular data. Therefore, this study proposed recognizing L. rigida as Palisada rigida comb. nov. Molecular data for L. heteroclada f. decussata on the other hand supported its separation from L. heteroclada, with too great a molecular distance to be considered a variety. Morphological characters that best separated P. rigida from L. decussata included seven characters; number of pericentral cells per vegetative axial segment, the presence of secondary pit connections, the presence of lenticular thickenings, tetrasporangia alignment, the presence of corps en cerise, holdfast morphology, and overall plant shape. Morphologically, L. heteroclada f. decussata was also separated from L. heteroclada, particularly by the following characteristics; ultimate branchlets morphologies, lower order branch lengths, primary axis and holdfast morphologies. Therefore, it was proposed that L. heteroclada f. decussata is recognized at a species level as L. decussata comb. et stat. nov.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.871-877
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• 2010

This study was conducted to investigate the effects of host tree species, temperature and humidity on ex vitro for seed germination in endangered species of Loranthus tanakae. In addition, we compared seed shapes between Loranthus tanakae and Viscum album that we could have easily shown in ex vitro condition. Seeds were germinated after one week inoculation and followed to develop radicles. Seed germination rates of Loranthus tanakae were 80~95% in most of the experimental conditions. The highest rate of holdfast penetrated to host plants was 72% in Populus alba var. pyramidalis among 13 different species tested at $20^{\circ}C$. Also the rates of their penetration were 57% in Morus bombycis, 55% in Acer palmatum and 42% in Castanea crenata at $20^{\circ}C$. Seeds were germinated under condition without irrigation and followed to withered in 12 weeks later. Stages of seed germination of the Loranthus tanakae were followed by radicle induction, holdfast development, haustorium formation and penetration in order in total period of 14 weeks.

• ALGAE
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• v.27 no.3
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• pp.139-153
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• 2012

The re-examination of morphological and anatomical characters of Laminaria appressirhiza and L. inclinatorhiza collected from different localities in the Sea of Okhotsk was performed. Despite their commercial and ecological importance to the region they have not been comprehensively reviewed since their first description in 1970. Our results show that some original diagnostic key characters (e.g., shape of holdfast, shape of sporangial sori, and dissection of blade) are not stable and have deviations from the type morphology when plants grow in different environments. In L. inclinatorhiza, the sporangial sori development occurred differently to the pattern indicated in original species description as they did not develop simultaneously on both sides of the blade. Instead, the sporangial sori outlines on both sides of the blade did not coincide at first and only became coincident later. Also, a deep-water population of L. inclinatorhiza with an unusual and interesting morphology, growing at depths of 15-25 m on opened rocky coasts in Taujskaya Bay (northern part of the Sea of Okhotsk) was found. The stable diagnostic key characters to distinguish these two species are the cone-like, multilayered, very thick and massive holdfast (in L. inclinatorhiza) and rolled margins of blades, lamellar rosette-like part of thallus, and sporangial sori developing only on one side of the blade (in L. appressirhiza). The ecological characteristics, distribution, and abundance of both species in the Sea of Okhotsk are discussed. Both species are perennial and widely distributed in the region. L. appressirhiza is more often found as a subdominant species among other kelps, forming maximum biomass and density of 7-9 kg and 8-25 plants per $1m^2$, respectively. L. inclinatorhiza sometimes forms local mono-species communities with maximum biomass and density of 10-12 kg and 10-15 plants per $1m^2$, respectively.

• Journal of Plant Biology
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• v.34 no.1
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• pp.45-51
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• 1991

Morphological variability was quantified for Endarachne binghamiae J. Agardh from five local populations collected in December, 1989 and in March, 1990. The sampling sites were chosen at intervals of 50'N along the east coast of Korea. Fifty individuals were haphazardly collected for the relationship between the blade length and reproduction, among which twenty five individuals were randomly selected for morphological variation. The Korean plants of E. binghamiae had the capacity of reproduction at an early stage of growth. The holdfast diameter and the plurangial length showed clinal variation of increment from the north to the south whereas the cryptostomata from the south to the north. The holdfast diameter, the blade thickness, the number of plurangial blades and the cryptostomata increased from December to March, when the blade length decreased. Endarachne is distinguished by the densely interwoven hypha I cells in the medulla, [our to eighteen rows of the plurangial compartments, and absence of paraphyses between plurangia. Therefore E. binghamiae should not be combined into Petalonia, but rather the Soviet plants of P. zosterijolia may be renamed as a taxon belonging to the genus Endarachne.rachne.