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Effects of Storage Duration, Medium and Viscin on in vitro seed Germination in Endangered Species, Loranthus tanakae  

Lee, Su-Gwang (Lab. of Environmental Biotechnology, Department of Biological & Environmental Science, College of Life Science and Biotechnology, Dongguk University)
Lee, Song-Hee (Lab. of Environmental Biotechnology, Department of Biological & Environmental Science, College of Life Science and Biotechnology, Dongguk University)
Kang, Ho-Duck (Lab. of Environmental Biotechnology, Department of Biological & Environmental Science, College of Life Science and Biotechnology, Dongguk University)
Publication Information
Journal of Korean Society of Forest Science / v.99, no.4, 2010 , pp. 618-624 More about this Journal
Abstract
This study was conducted to establish the in vitro seed germination of Loranthus tanakae. A factorial experiment evaluated the effects of seed storage duration (0, 8, 16 weeks), media (MS, SH, White, WPM), presence of viscin, GA3, gelling agent and concentrations. Seed germinated after one week culture in in vitro condition and produced radicles. In vitro seed germination was optimal when the seeds removed viscin placed on SH medium (69%) and the addition of 0.35% gelrite (75%) in the same medium. As seed storage duration was expanded to 8 or 16 weeks, in vitro seed germination rate was reduced rapidly. Holdfasts were also produced at the side of radicles. The important factors to produce holdfast and haustorium was kind of media as an optimal condition in White medium without any supplements to be shown 98% and 8% respectively. Process of in vitro germination of Loranthus tanakae was followed to radicle elongation, holdfast development and then haustorium formation sequencially.
Keywords
Loranthus tanakae; in vitro germination; viscin; holdfast; haustorium;
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Times Cited By KSCI : 2  (Citation Analysis)
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