• Korean Journal of Ichthyology
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• v.12 no.3
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• pp.192-202
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• 2000

Reproductive biology of the slimy, Leiognathus nuchalis was investigated by means of histological methods. Sex ratio was 1.81 : 1 in female to male. Developmental pattern of oocytes was group-synchronous. Testicular structure was restricted spermatogonial testis-type of tubular testis. The size of first group maturity is 7.5 cm in total length. Gonadosomatic index(GSI) of female was the highest in July(12.83) and the lowest in September(1.08). GSI of male was the highest in June(19.0) and the lowest in October(0.24). Hepatosomatic index(HSI) of female showed to be positively correlated with GSI. Thoracic spot index(TSI) showed to be the minimum value from May to July when the maturation and ripe season of gonad. Reproductive cycle of female could be classified into the growing(March~May), maturation(May~June), ripe and spent(June~August), recovery(August~November), and resting stage(November~March). Reproductive cycle of male could be classified into the multiplicative and growing(January~April), maturation(April~May), ripe and spent(June~August), recovery(August~October), and resting stage(October~December).

• Journal of Aquaculture
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• v.14 no.4
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• pp.205-211
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• 2001

Scapharca satawi is dioecious and attains sexual maturity, when it grows to a shell length of 70 mm. Its ovary is composed of a number of ovarian sacs; likewise, its testis is also composed of several testicular tubules. During the year 1999-2000, the condition index reached the maximum (0.017) in June and spawning occurred from July to September, when water temperature remained above 23$^{\circ}C$. The reproductive cycle of S. satowi can be classified into five successive stages: early active (December to March), late active (April to May), ripe (June 7o July), partially spawned (July to September) and spent/inactive (September and November) stages. Monthly changes in the condition index were closely relaxed to its reproductive cycle.

• The Korean Journal of Malacology
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• v.18 no.2
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• pp.67-76
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• 2002

Gametogenesis, reproductive cycle, egg diameter composition and gonad index of the black-lined limpet, Cellana nigrolineata, were histologically examined. Specimens collected from the rocky intertidal zone of Hamdeok, Jeju from July 1997 to June 1998. The black-lined limpet has an unpaired gonad located at the ventro-anterior part, underneath the visceral mass. The gonad index increased from May when water temperature increased and reached maximum in August. It began to decrease from September thereafter, maintain a low value from January to April. The following reproductive cycle were classified based on the monthly changes of the histological features and sizes of oocytes in the gonad: recovery stage (November to April), active stage (March to June), ripe stage (May to October), spent stage (August to December). Spawning occur once a year between September and October. The black-lined limpet appeared to be gonochorism, neither sex change nor hermaphroditie.

• Journal of Aquaculture
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• v.2 no.1
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• pp.21-32
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• 1989

This study was undertaken to understand development stage of ovary and changes of hormone concentrations in the Israeli carp, Cyprinus carpio from Ferbruary to May. The results obtained in these experiments are as follows: 1. Serum LH levels began to increase sharply in March, coinciding with the onset of rapid ovarian development. 2. LH levels were well correlated with changes in gonadosomatic index. 3. Dramatic increase in gonadosomatic index occured during the months of March. 4. Ripe stage(Stage VII) rapidly increase in March. 5. Early perinucleolus oocyte rapidly develop into late perinucleolus oocytes in March. 6. The vitellogenic phase begins as these late perinucleolus oocytes become transformed into early maturing oocytes through the accumulation of yolk. 7. The cytoplasm completely fills with yolk as oocytes reach the late maturing stage. 8. Changes in the microscopic appearances of the ovaries were well correlated with changes in both gonadosomatic index and macroscopic appearance. 9. It is concluded from these observations that LH plays a major role in sexual maturating of the Israeli carp.

• The Journal of Korean Obstetrics and Gynecology
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• v.37 no.2
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• pp.35-57
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• 2024

Objectives: This systematic review of animal studies was designed to examine the therapeutic mechanisms of herbal medicines of insulin resistance in Polycystic ovary syndrome and composition of them. Methods: Studies were searched from PubMed, CNKI and Medline up to February 2024. Data was extracted and organized as animal model characteristics, treatment period, experimental and control group settings, evaluation index of treatment, therapeutic mechanism of herbal medicine and Composition of prescription. Results: In the 15 studies finally selected, the herbal medicine used in the study was effective in regulating the insulin resistance index, sex hormones and blood lipid index and improving the histological morphology of the ovaries. The herbal medicine prescriptions used in each of the 15 papers were different, and Salviae Miltiorrhizae Radix (丹參), Wolfiporia cocos (茯苓) and Epimedii Herba (淫羊藿) were most frequently used herbs, 7 times. Conclusions: The results showed that herbal medicine is effective in treating insulin resistance in Polycystic ovary syndrome through several mechanisms. The herbs used in more than 4 papers had effects such as anti-inflammatory, anti-oxidant, estradiol hormone production control and glycolipid metabolism control. More diverse clinical studies on insulin resistance in Polycystic ovary syndrome are needed.

• Journal of Korean Neurosurgical Society
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• v.29 no.9
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• pp.1215-1221
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• 2000

Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.5
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• pp.404-409
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• 2006

This study investigated the reproductive cycle of the female hairychin goby, Sagamia geneionema, histologically. The fecundity of female hairychin goby ranged from 1,002 to 1,240 eggs when they reached a total length of 9.1-10.0 cm. Fecundity is related to total length. The gonadosomatic index (GSI) increased from December (1.87$\pm$0.46) and reached a maximum in April (11.57$\pm$1.92). The histological changes in the ovary were correlated with the GSI. Oocytes at the chromatin-nucleolus and peri-nucleolus stages were observed in the ovary year-round. In December, oocytes containing yolk appeared in the ovaries of a few fish. Most oocytes appearing in January were at the yolk globule stage. The frequency of oocytes appearing at the yolk globule stage from January to March was higher than in other months. Subsequently, empty follicles and atretic oocytes were observed in the ovaries in May. Based on the histological observations of gonad development and the monthly change in the GSI, the reproductive cycle was classified into the following successive stages: growing (October to November), mature (December to January), ripe and spawning (February to April), and degenerative and resting (May to September) stages. The histological observations of ovaries during the spawning period indicate that this species is a multiple spawner with abbreviated iteroparity based on the developmental pattern of oocytes.

• Journal of Acupuncture Research
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• v.23 no.3
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• pp.129-142
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• 2006

Objectives : The purpose of this study is to observe the effects of Eucomiae Cortex herbal-acupuncture solution(EC-HAS) at Joksamni(ST36) on arthritis of mice induced by Collagen II. Methods : The author performed several experimental items. First, it is the cell survival rate of mice lung fibroblasts. Second, it is the incidence rate of arthritis and arthritis index of CIA. Third, it is the levels of IL-6, $TNF-{\alpha}$, $IFN-{\gamma}$, $IL-{\beta}$, IgG, IgM and anti-collagen II in serum and the level of IFN-y,$IFN-{\gamma}$/IL -4 ratio in CIA mouse spleen cell culture. Fourth, it is histological analysis of the mice joint. Fifth, it is expression ratio of $CD3e^+$ to $CD19^+$+ cell, $CD4^+$ to $CD8^+$ cell, $CD69^+/CD3e^+$/cells, $CD11a^+/CD19^+$/cells, $CD11b^+/Gr-1^+$ cells and $CD4^+/CD25^+$ cells. Results & Conclusion : 1. In the EC-HA, the incidence of arthritis and arthritis index were significantly decreased. 2. In EC-HA, the levels of IL-6, $IFN-{\gamma}$, $TNF-{\alpha}$, $IL-1{\beta}$, IgG, IgM and anti-collagen II in serum of CIA mice and the level of $IFN-{\gamma}$, IL-4, $IFN-{\gamma}$/lL-4 ratio in CIA mouse spleen cell culture were significantly decreased. 3. In the histological study, the cartilage destruction and synovial cell proliferation were decreased in the EC-HA, and the collagen fiber expressions in the EC-HA were similar with that of the Normal group. 4. In the EC-HA, the expression ratio of $CD3e^+$ to $CD19^+$ cell and $CD4^+$ to $CD8^+$ cell were similarly maintained as Normal group in lymph nodes, and $CD69^+/CD3e^+$ cells and $CD11a^+/CD19^+$ cells were decreased in lymph nodes, and $CD11b^+/Gr-1^+$ cells and $CD4^+/CD25^+$ cells were decreased in synovium. These results suggest that EC-HA at ST36 has an effect to control synovial cell proliferation and cartilage destruction in rheumatoid arthritis, and to be put to practical use in the future rheumatoid arthritis clinic.

• Journal of Society of Preventive Korean Medicine
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• v.3 no.1
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• pp.1-33
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• 1999

In order to compare and study the effects of Kamipalmijiwhang tang(加味八味地黃湯) & Kamigangwhalsokdantang(加味羌活續斷湯) on inhibition of osteoporosis induced by ovariectomy, the author administered Kamipalmijiwhangtang & Kamigangwhalsokdantang to the sample groups per orally before and after ovariectomy. The author observed the activities of cathepsin L, K and the contents of calcium, calcitonin, osteocalcin and phosphorus in the serum for the biochemical index, the contents of IL-6 in the serum and bone marrow for immunological index, the state of bone loss in the metaphysis of tibia by histological study, the expression of ELAM-1 &collagen IV by immunohistochemical analysis, the expression of fos by immunocytochemical analysis. The results were as follows: 1. The activities of cathepsin L, K in the serum had a significant inhibitive effects in all sample groups compared with the control group. The inhibitive effects of Sample A-1 & B-1 was better than those of A-2 & B-2 and the inhibitive effects of sample B-1 & B-2 was better than those of A-1 & A-2. 2. The content changes of IL-6 in the serum and bone marrow had a significant inhibitive effects in all sample groups compared with the control group. The inhibitive effects of sample A-1 & B-1 was better than those of A-2 & B-2 and the inhibitive effects of sample B-1 & B-2 was better than those of A-1 & A-2. 3. In the histological study of tibial metaphysis all the sample groups inhibited bone loss significantly compared with the control group and expressed trabecula shape of bone similar to the normal group. 4. The expression of ELAM-1 & collagen IV decreased a little bit in sample A-2. It also decreased to a similar degree in sample B-2 like normal group and disturbed itself. 5. The expression of fos was inhibited in all sample groups compared with control group. The inhibitive expression of sample B-1 & B-2 was more remarkable than those of A-1. & A-2. 6. The serum calcium had a significant increasing effects in an sample groups compared with control group. Each sample groups didn't have any significant difference. 7. The serum calcitonin had a significant increasing effects in sample A-1, A-2 and B-1 groups compared with control group. Each sample groups didn't have any significant difference. 8. The serum osteocalcin and phosphorus didn't have any significant difference in all sample groups compared with control group.

• Journal of Aquaculture
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• v.9 no.1
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• pp.73-81
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• 1996

This study was conducted to study the reproductive cycle in female fusilier, Caesio diagramma by using the histological methods. Histological changes of the ovary were well correlated with the patterns of gonadosomatic index (GSI) and hepatosomatic index (HSI). GSI was increased in April as the value of HSI increase and reached to its maximum in May and June. Oocytes at the chromatin-nucleolus and peri-nucleolus stages were observed in the ovary throughout the year. In April, oocytes containing yolk appeared in ovaries of a few fishes. Most oocytes appearing in May and June belonged to the tertiary yolk stage. Frequency of oocytes appearance at the tertiary yolk stage in May and June was higher than that of the other months. Moreover, the empty follicles and atretic oocytes were observed in the ovaries with many vitellogenic oocytes during these two months. Thereafter, oocytes of the yolk stage disappeared in September. The spawning period of C. diagramma is from the month of April to June, and this species belongs to an asynchronous and multiple spawner.