• 대한본초학회지
• /
• 제32권3호
• /
• pp.79-87
• /
• 2017

Objectives : This study aimed to investigate the protective effects of an herbal mixture of Atractylodes macrocephala and Taraxacum spp. (ATC) on ulcerative colitis. We have previously screened traditional medicinal herbs to discover the effective candidate by the animal model. A. macrocephala and T. spp were identified as one of the effective herbs in the screening process. Methods : Experimental colitis was induced in male Balb/c mice by administering drinking water containing dextran sulfate sodium, which mimics the clinical and histological features of ulcerative colitis in human. ATC at doses of 30, 100 or 300 mg/kg were orally administered to mice twice per day for 10 consecutive days. To evaluate the damage from experimentla ulcerative colitis, body weight, colon length, disease activity index, myeloperoxidase and histological changes were measured and analyzed. Results : The administration of dextran sulfate sodium with drinking water resulted in markedly reduced colon length, severe body weight loss, increased levels of myeloperoxidase activity and histological damages in mice. ATC treatment significantly ameliorated the colon shortening, histological damage, body weight loss and disease activity index score in a dose-dependent manner. ATC also attenuated the colonic myeloperoxidase activity which reflects the severity and extent of inflammatory damage of colon. Conclusions : ATC exerts protective effects against inflammatory colonic structural damage induced by epithelial barrier integrity impairment. ATC also inhibits weight loss and related symptoms of UC which can be considered as the functional recovery of colon.

• 보존과학연구
• /
• 통권35호
• /
• pp.87-98
• /
• 2014

뼈의 보존 상태를 화학적으로 평가하기 위하여 조선시대 인골 10점을 대상으로 감쇠전반사법을 활용한 적외선 분광분석(FTIR-ATR)을 실시하였다. FTIR-ATR 스펙트럼을 통해 결정화 지수(crystallinity index; CI), 탄산염간의 비(C/C), 탄산염과 인산염의 비(C/P)를 계산한 결과 CI는 $4.25{\pm}0.78$, C/C는 $0.91{\pm}0.04$, C/P 는 $0.19{\pm}0.06$으로 확인되었다. 화학적 평가 결과 조직학 지수가 높을수록 CI와 C/P 값이 증가하며, C/C는 감소하는 것을 확인하였다. 반면 뼈에서 추출한 DNA 분석 가능 여부는 C/C가 낮고 C/P가 높을수록 분석이 가능 하였다. 이를 통해 FTIR을 이용한 화학적 평가와 조직 분석 결과가 출토 인골의 보존 상태를 종합적으로 파악하기 위한 기준으로 적용이 가능할 것으로 판단된다.

• Journal of Chest Surgery
• /
• 제47권5호
• /
• pp.458-464
• /
• 2014

Background: The effort to detect lung cancer in ever-earlier stages leads to the identification of an increasing number of patients without preoperative histological diagnosis. The aim of this study is to determine the prevalence and characteristics of benign lesions excised in the context of lung cancer surgery. Methods: We retrospectively analyzed data from 125 surgical procedures. We compared the preoperative clinical or cyto-histological diagnosis with the surgical-pathologic diagnosis in order to identify the percentage of benign lesions excised. Furthermore, other parameters were analyzed, such as age, sex, tumor size, the presence of calcification, and the type of surgery according to subgroup. Results: Of the 125 patients included in the study, 63 (50.4%) had a preoperative histological diagnosis of malignancy, corresponding to 56 cases (44.8%) of primary lung cancer and 7 cases (5.6%) of metastases. The 62 (49.6%) remaining cases without preoperative histological diagnosis were divided among 50 (40%) solitary pulmonary nodules and 12 (9.6%) pulmonary masses. According to the postoperative pathologic examination, we identified 12 (9.6%) benign lesions excised during lung cancer surgery. There were no statistically significant differences by subgroups with respect to age or sex. We found statistically significant evidence regarding the size and wedge resection as the surgical technique of choice for this type of benign lesion. Conclusion: Our study obtained results similar to those published by other groups regarding the resection of benign lesions in lung cancer surgery. This percentage could be a quality management index of indeterminate lung lesions.

• 한국어류학회지
• /
• 제18권2호
• /
• pp.87-96
• /
• 2006

이사천에서 채집된 칼납자루의 생식과 기관계 구조를 조직학적인 방법에 의하여 조사한 결과는 다음과 같다. 암:수 성비는 1:1로 나타났다. 암컷의 경우 생식소중량지수(GSI)는 12월과 4월에 두 번의 정점을 나타냈으며, 산란관지수(OLI)는 불규칙한 변화 양상을 보였다. 기관계의 조직학적인 관찰 결과, 피부에서는 상피층의 위축과 색소세포의 증가가 확인되었으며, 아가미에서는 새판 상피층의 박리와 새엽의 분지현상이 나타났다. 그리고 간과 신장에서는 울혈 및 간세포의 세포질 변성과 사구체의 팽창, 세뇨관 상피세포의 초자성 변성 및 간질세포의 핵 응축이 관찰되었다.

• 한국수산과학회지
• /
• 제55권5호
• /
• pp.688-696
• /
• 2022

Obesity could cause immune-physiological disorders in fish. Yet, little is known about the impact of obesity on stress and histological responses. This study aimed to determine histological and physiological changes in and vulnerability of overfeeding-induced obese rainbow trout Oncorhynchus mykiss exposed to stress condition. Control, intermediate and overfed groups were fed at 1.5, 2.5 and 3.09% of their body weight per day, respectively, for eight weeks. Weight gain, body mass index, hepatosomatic index and serological parameters, and histology of liver were measured in five fish from each group at week 0, 2, 4, and 8. At week 8, 20 fish from each group were exposed to heat stress by increasing water temperature at a rate of 3℃ per day from 15 to 25℃ and maintaining the final temperature for 10 days. Overall, overfed fish showed significantly higher weight gain, body mass index, and serological parameters than those of fish in the other groups. Fish in the overfed and intermediate groups displayed multifocal infiltration of inflammatory cells in hepatic parenchyma. Mortality rate and serological parameters of fish in the overfed group exposed to heat stress were significantly higher than those of fish in the other groups, indicating increased vulnerability to environmental stress.

• 대한한의학회지
• /
• 제21권3호
• /
• pp.199-208
• /
• 2000

Objectives : In order to investigate the toxicity of rats after oral administration of Eumcheonyijin-tang extract. Methods : The experimental animals were subdivided into control, short term administration group, and long term administration group. With changes of gross appearance, the histological changes of liver and kidney were observed. Blood chemical indexes used in this study were AST, ALT, total bilirubin, albumin, BUN and creatinine in serum. Results : In the long term administration group, histological changes were detected in the liver as centrolobular disposition of fatty tissue(adipose cell), and in serum test, AST, ALT increased at 21 days after administration group, serum total bilirubin were increased 21, 28 and 35 days after administration group. So it seems to induce toxicity. Kidneys of the long term administration group revealed histological changes : increasing of connective tissue and pyknosis of glomerulus cell were observed at 28 days after administration group, and in serum test, significant changes of albumin, BUN, and creatinine were admitted. So it seems to induce toxicity. Conclusions : In long term administration of Eumcheonyijin-tang toxicity was induced.

• 생약학회지
• /
• 제44권4호
• /
• pp.391-396
• /
• 2013

In this study, we investigated the effects of onion(Allium cepa L.) peel extraction aplication on UVB-induced damage of mouse skin. The male C57BL/6 weeks mice were divided into three groups; the control group(Con), the UVB irradiated group(UVB) and the group treated with onion peel extract after UVB irradiation(UVB+Onion peel). Onion peel extraction were topically treated after UVB irradiation(800 $mJ/cm^2$) to dorsal skin. We were measured TEWL, melanin value, erythema index and histological of mouse skin. In the TEWL, melanin value and erythema index observation, UVB+onion peel group were decreased then in the UVB group and 120 and 168 hr groups were similar to the control group. In the histological observation, UVB+onion peel group were indicated hyperkeratosis then in the UVB. These results showed that onion peel extract as a topical application may have preventive effect against UVB-induced skin damage. Therefore onion peel extract might be good material for UVB-damage skin care.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권13호
• /
• pp.5319-5323
• /
• 2015

Background: The purpose of this study was to evaluate the prognostic significance of Ki-67 and subjective microvascular density (SMVD) indexes together with other factors in patients with oligodendroglioma. Materials and Methods: In this retrospective study, oligodendroglioma specimens obtained from twenty-five consecutive patients were evaluated for Ki-67 and SMVD indices to help determine histological grading and investigate the fidelity of these markers in clinical prognosis. Other potentially prognostic factors were Karnofsky performance scale, tumor histological grade, and adjuvant radiotherapy. Results: The Ki-67 proliferation index appeared to have a strong correlation with the grade of the tumor and the survival. Age, gender, adjuvant radiotherapy, surgical resection type (complete versus incomplete) did not have any influence on recurrence. The SMVD index correlated significantly with the 3 to 5-year survival. Conclusions: Ki-67 and MVD indexes are important and useful markers in estimating the prognosis of oligodendrogliomas.

• Environmental Analysis Health and Toxicology
• /
• 제21권1호
• /
• pp.35-43
• /
• 2006

Reproduction and organ structure of the pale chub, Zacco platypus in Isa stream were investigated by means of histological methods. The results of the study confirmed reproductive abnormality and histopathological features in the pale chub. The gonadosomatic index (GSI) of the fish showed two peak in April and August. In summer season, GSI of the male was about two times of the female's GSI. Monthly variation of the gonadal development was very irregular. From the histological analysis of the organ structure, epidermal atrophy, necrosis and hyperplasia of pigment cell were observed in the skin. Epithelial layer lifting and clubbing of the lamella and bifurcation of the filament were observed in the gill. Also histological changes as congestion, cytoplasmic degeneration of hepatic cell, degeneration of bile duct, glomerular dilatation, degeneration of renal tubule and pycnosis of interstitial cell were identified in the liver and kidney, respectively.

• 한국발생생물학회지:발생과생식
• /
• 제7권1호
• /
• pp.23-28
• /
• 2003

2000년 2월부터 2001년 2월까지 남태평양 미크로네시아 군도의 Chuju 연안에 서식하는 주Epinephelus merra 암컷을 대상으로 생식주기를 조사하였다. 생식소숙도지수 (GSI)는 2월부터 증가하기 시작하여 3월에 최고치 (3.41$\pm$0.84)를 보였다. 조직학적 관찰결과 3월과 4월의 난소 안에는 난황을 가진 다양한 단계의 난모세포와 배란여포들이 존재하였다. 6월부터 1월에 난소 안에는 미성숙 난모세포만 있었다. 이들 결과로 E. merra의 산란 시기는 3월과 4월로 유추할 수 있었다.