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http://dx.doi.org/10.6116/kjh.2017.32.3.79.

Protective Effect of Atractylodes macrocephala and Taraxacum spp. Combination Treatment in Balb/c Mice with Dextran Sulfate Sodium-Induced Ulcerative Colitis  

Lee, Donghun (Kyung Hee University, College of Korean Medicine, Department of Herbal Pharmacology)
Kim, Hocheol (Kyung Hee University, College of Korean Medicine, Department of Herbal Pharmacology)
Publication Information
The Korea Journal of Herbology / v.32, no.3, 2017 , pp. 79-87 More about this Journal
Abstract
Objectives : This study aimed to investigate the protective effects of an herbal mixture of Atractylodes macrocephala and Taraxacum spp. (ATC) on ulcerative colitis. We have previously screened traditional medicinal herbs to discover the effective candidate by the animal model. A. macrocephala and T. spp were identified as one of the effective herbs in the screening process. Methods : Experimental colitis was induced in male Balb/c mice by administering drinking water containing dextran sulfate sodium, which mimics the clinical and histological features of ulcerative colitis in human. ATC at doses of 30, 100 or 300 mg/kg were orally administered to mice twice per day for 10 consecutive days. To evaluate the damage from experimentla ulcerative colitis, body weight, colon length, disease activity index, myeloperoxidase and histological changes were measured and analyzed. Results : The administration of dextran sulfate sodium with drinking water resulted in markedly reduced colon length, severe body weight loss, increased levels of myeloperoxidase activity and histological damages in mice. ATC treatment significantly ameliorated the colon shortening, histological damage, body weight loss and disease activity index score in a dose-dependent manner. ATC also attenuated the colonic myeloperoxidase activity which reflects the severity and extent of inflammatory damage of colon. Conclusions : ATC exerts protective effects against inflammatory colonic structural damage induced by epithelial barrier integrity impairment. ATC also inhibits weight loss and related symptoms of UC which can be considered as the functional recovery of colon.
Keywords
Atractylodes macrocephala; Taraxacum spp.; ulcerative colitis;
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1 Head KA, Jurenka JS. . 2003. Inflammatory bowel disease Part 1: ulcerative colitis--pathophysiology and conventional and alternative treatment options. Alternative medicine review : a journal of clinical therapeutic 8: 247-83.
2 Loftus EV,Jr. . 2004. Clinical epidemiology of inflammatory bowel disease: Incidence, prevalence, and environmental influences. Gastroenterology 126: 1504-1517.   DOI
3 Campieri M, Gionchetti P. . 2001. Bacteria as the cause of ulcerative colitis. Gut 48: 132-135.   DOI
4 Sartor RB. . 2006. Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis. Nature clinical practice Gastroenterology & hepatology 3: 390-407.   DOI
5 Fournier BM, Parkos CA. . 2012. The role of neutrophils during intestinal inflammation. 5: 354-366.   DOI
6 Burger D, Travis S. . 2011. Conventional medical management of inflammatory bowel disease. Gastroenterology 140: 1827-1837.e2.   DOI
7 Xu CT, Meng SY, Pan BR. . 2004. Drug therapy for ulcerative colitis. World J Gastroenterol 10: 2311-2317.   DOI
8 China. CftPoPsRo. Pharmacopoeia of The People's Republic of China2010.
9 Kiso Y, Tohkin M, Hikino H. . 1983. Antihepatotoxic principles of Atractylodes rhizomes. J Nat Prod 46: 651-654.   DOI
10 Wang R, Zhou G, Wang M, Peng Y, Li X. . 2014. The Metabolism of Polysaccharide from Atractylodes macrocephala Koidz and Its Effect on Intestinal Microflora. Evid Based Complement Alternat Med 2014: 926381.
11 Liu H, Zhu Y, Zhang T et al. . 2013. Anti-tumor effects of atractylenolide I isolated from Atractylodes macrocephala in human lung carcinoma cell lines. 18: 13357-68.   DOI
12 Zhang J, Cao G, Xia Y, Wen C, Fan Y. . 2014a. Fast analysis of principal volatile compounds in crude and processed Atractylodes macrocephala by an automated static headspace gas chromatographymass spectrometry. Pharmacognosy magazine 10: 249-53.
13 Schutz K, Carle R, Schieber A. . 2006. Taraxacum-a review on its phytochemical and pharmacological profile. J Ethnopharmacol 107: 313-323.   DOI
14 Shin HS, Satsu H, Bae MJ, Zhao Z, Ogiwara H, Totsuka M, et al. Anti-inflammatory effect of chlorogenic acid on the IL-8 production in Caco-2 cells and the dextran sulphate sodium-induced colitis symptoms in C57BL/6 mice. Food Chem. 2015;168:167-75.   DOI
15 Gonzalez-Castejon M, Visioli F, Rodriguez-Casado A. . 2012. Diverse biological activities of dandelion. Nutrition reviews 70: 534-47.   DOI
16 Ye H, Chen C, Zhu S. . 2014. Effects of baizhu decoction on ulcerative colitis model rats and the serum IL - 6, IL - 17. Journal of Shaanxi college of traditional Chinese medicine 1: 69-71.
17 Leenen CH, Dieleman LA. . 2007. Inulin and oligofructose in chronic inflammatory bowel disease. J Nutr 137: 2572S-2575S.   DOI
18 Johansson ME, Gustafsson JK, Sjoberg KE et al. . 2010. Bacteria penetrate the inner mucus layer before inflammation in the dextran sulfate colitis model. PloS one 5: e12238.   DOI
19 Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology. 1990;98(3):694-702.   DOI
20 Cooper HS, Murthy SN, Shah RS, Sedergran DJ. . 1993. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Laboratory investigation; a journal of technical methods and pathology 69: 238-49.
21 Kitajima S, Takuma S, Morimoto M. . 2000. Histological analysis of murine colitis induced by dextran sulfate sodium of different molecular weights. Exp Anim 49: 9-15.   DOI
22 Jadert C, Phillipson M, Holm L, Lundberg JO, Borniquel S. . 2014. Preventive and therapeutic effects of nitrite supplementation in experimental inflammatory bowel disease. Redox biology 2: 73-81.   DOI
23 Videla S, Vilaseca J, Antolin M et al. . 2001. Dietary inulin improves distal colitis induced by dextran sodium sulfate in the rat. Am J Gastroenterol 96: 1486-1493.   DOI
24 Bradley PP, Priebat DA, Christensen RD, Rothstein G. . 1982. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. The Journal of investigative dermatology 78: 206-9.   DOI
25 Yan Y, Kolachala V, Dalmasso G et al. . 2009. Temporal and spatial analysis of clinical and molecular parameters in dextran sodium sulfate induced colitis. PloS one 4: e6073.   DOI
26 Kang TH, Bang JY, Kim MH, Kang IC, Kim HM, Jeong HJ. . 2011. Atractylenolide III, a sesquiterpenoid, induces apoptosis in human lung carcinoma A549 cells via mitochondria-mediated death pathway. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 49: 514-9.   DOI
27 Kim YH, Choo SJ, Ryoo IJ, Ahn JS, Yoo ID. . 2011. Eudesmanolides from Taraxacum mongolicum and their inhibitory effects on the production of nitric oxide. Archives of pharmacal research 34: 37-41.   DOI
28 Zhang X, Xiong H, Li H, Cheng Y. . 2014b. Protective effect of taraxasterol against LPS-induced endotoxic shock by modulating inflammatory responses in mice. Immunopharmacology and immunotoxicology 36: 11-6.   DOI
29 Kitajima S, Takuma S, Morimoto M. . 1999. Tissue distribution of dextran sulfate sodium (DSS) in the acute phase of murine DSS-induced colitis. J Vet Med Sci 61: 67-70.   DOI
30 Roediger WE. . 1990. The starved colon--diminished mucosal nutrition, diminished absorption, and colitis. Diseases of the colon and rectum 33: 858-62.   DOI
31 Fakhoury M, Negrulj R, Mooranian A, Al-Salami H. . 2014. Inflammatory bowel disease: clinical aspects and treatments. Journal of inflammation research 7: 113-20.
32 Gottfries J, Melgar S, Michaelsson E. . 2012. Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease. PloS one 7: e30005.   DOI
33 Kawada M, Arihiro A, Mizoguchi E. . 2007. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease. World J Gastroenterol 13: 5581-5593.   DOI
34 Egger B, Bajaj-Elliott M, MacDonald TT, Inglin R, Eysselein VE, Buchler MW. . 2000. Characterisation of acute murine dextran sodium sulfate colitis: cytokine profile and dose dependency. Digestion 62: 240-248.   DOI
35 Shan GS, Zhang LX, Zhao QM et al. . 2014. Metabolomic study of raw and processed Atractylodes macrocephala Koidz by LC-MS. J Pharm Biomed Anal 98: 74-84.   DOI
36 Wang R, Zhou G, Wang M, Peng Y, Li X. . 2014. The Metabolism of Polysaccharide from Atractylodes macrocephala Koidz and Its Effect on Intestinal Microflora. Evid Based Complement Alternat Med 2014: 926381.
37 Jin YR, Jin J, Piao XX, Jin NG. . 2011. The effect of Taraxacum spp. on gastric emptying and smooth muscle motility in Rodents. Neurogastroenterol Motil 23: 766-e333.   DOI
38 Murthy SN, Cooper HS, Shim H, Shah RS, Ibrahim SA, Sedergran DJ. . 1993. Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin. Digestive diseases and sciences 38: 1722-34.   DOI
39 Kumar GK, Dhamotharan R, Kulkarni NM, Honnegowda S, Murugesan S. . 2011. Embelin ameliorates dextran sodium sulfate-induced colitis in mice. International immunopharmacology 11: 724-31.   DOI
40 Kim SH, Jung HN, Lee KY, Kim J, Lee JC, Jang YS. . 2005. Suppression of Th2-type immune response-mediated allergic diarrhea following oral administration of traditional Korean medicine: Atractylodes macrocephala Koidz. Immunopharmacology and immunotoxicology 27: 331-43.   DOI