• 한국자원식물학회지
• /
• 제25권6호
• /
• pp.682-692
• /
• 2012

Sea Buckthorn (Hippophae rhamnoides L.) has been used in traditional medicine for the treatment of cough, indigestion, circulatory problems and pain. The associated anti-inflammatory effect of this agent is achieved via the inhibition of Nf-${\kappa}B$ signaling, a property that has been demonstrated to effectively control the symptoms of various skin disorders, including atopic dermatitis. Accordingly, the purpose of this study was to assess the efficacy of Sea Buckthorn in reducing the production of lipopolysaccharide (LPS) activated nitric oxide (NO) by inhibiting the Nf-${\kappa}B$ pathway, as measured by the symptoms of atopic dermatitis (AD) occurring secondarily to inflammation and immune dysregulation. Our data demonstrate that Sea Buckthorn significantly decreased the LPS-induced production of NO (p<0.001). Atopic dermatitis was induced by repeated application of 2,4-dinitrochlorobenzene to the dorsal skin of mice. Topical application of 5% Sea Buckthorn extract improved the symptoms of AD, specifically reducing disease severity scores, scratching behaviors and epidermal thickness. When compared to the control group, animals treated with Sea Buckthorn exhibited increased serum IL-12 levels and decreased serum TNF-${\alpha}$, IL-4 and IL-5 levels. Such a modulation of biphasic T-helper (Th)1/Th2 cytokines may result in a reduction in serum IgE levels. Our findings suggest that mechanism of action of Sea Buckthorn in the treatment of AD is associated with a marked anti-inflammatory effect as well as an inhibition of Th2-mediated IgE overproduction via the modulation of biphasic Th1/Th2 cytokines. Such results suggest that topical Sea Buckthorn extract may prove to be a novel therapy for AD symptoms with few side effects.

• 한국식품과학회지
• /
• 제53권1호
• /
• pp.55-62
• /
• 2021

본 연구에서는 에탄올 농도를 달리한 비타민나무 잎 추출물의 항산화 물질 및 항산화 활성을 측정하였다. 에탄올 농도별 비타민나무 잎 추출물의 총 페놀 함량은 40% 에탄올 추출물과 80% 에탄올 추출물이 각각 285.06, 285.64 mg GAE/g으로 높은 함량을 보였으며, 총 플라보노이드, 총 비타민 C, 클로로필 a, 클로로필 b, 총 클로로필 함량은 각각 100% 에탄올 추출물이 123.78 mg NE/g, 807.11 mg/100 g, 30.65, 49.20, 77.58 g/mL로 높은 함량을 보였다. 항산화 활성은 40% 에탄올 추출물과 80% 에탄올 추출물이 DPPH 라디칼 소거능(IC50 49.18, 49.03 ㎍/mL), ABTS+ 라디칼 소거능(IC50 67.52, 65.71 ㎍/mL), 아질산염 소거능(IC50 5.15, 4.24 ㎍/mL) 및 환원력(0.90-2.60, 0.97-2.73 O.D.)에서 가장 활성이 좋았고 superoxide- 라디칼 소거능은 40% 에탄올 추출물이 IC50 60.80 ㎍/mL로 활성이 좋았다. 또한 아질산염 소거능과 ABTS+ radical 소거능의 경우 비교군보다 활성이 좋거나 비슷하여 비타민나무 잎의 천연 항산화 물질로의 활용 가능성을 확인하였다. 항산화 물질과 항산화 활성 간의 상관관계를 분석한 결과 상관계수가 0.715-0.990으로 양의 상관관계를 나타냈다. 따라서 비타민나무 잎 추출물 제조 시 40% 에탄올 및 80% 에탄올 용매를 사용할 경우 높은 항산화 활성을 나타내어 천연 항산화제로 활용 가능성이 높을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
• /
• 제26권3호
• /
• pp.596-602
• /
• 2016

Staphylococcus aureus, like other gram-positive pathogens, has evolved a large repertoire of virulence factors as a powerful weapon to subvert the host immune system, among which alpha-hemolysin (Hla), a secreted pore-forming cytotoxin, plays a preeminent role. We observed a concentration-dependent reduction in Hla production by S. aureus in the presence of sub-inhibitory concentrations of isorhamnetin, a flavonoid from the fruits of Hippophae rhamnoides L., which has little antibacterial activity. We further evaluate the effect of isorhamnetin on the transcription of the Hla-encoding gene hla and RNAIII, an effector molecule in the agr system. Isorhamnetin significantly down-regulated RNAIII expression and subsequently inhibited hla transcription. In a co-culture of S. aureus and lung cells, topical isorhamnetin treatment protected against S. aureus-induced cell injury. Isorhamnetin may represent a leading compound for the development of anti-virulence drugs against S. aureus infections.

• 한국축산식품학회지
• /
• 제38권3호
• /
• pp.468-475
• /
• 2018

The present trial was carried out to study the effect of sea buckthorn seed supplementation on egg quality of laying birds. A total of 160 Rhode Island Red${\times}$Fayoumi layers was divided into four groups of 40 birds each, which was further replicated four times with 10 hens per replicate. Group one was kept as a control, while other three groups were supplemented with sea buckthorn seed powder at a dose rate of 1 (T1), 2 (T2) and 3 (T3) g/kg of feed. The results showed that egg production was significantly (p<0.05) higher in T3 at the end of the study. Egg weight was significantly (p<0.05) high in T2 and T3 during week 39 and 40. Egg yolk weight was significantly (p<0.05) in T3 compared to the control. Significantly (p<0.01) lower egg cholesterol was recorded in T2 and T3. From the results of the present study, we concluded that laying hens supplemented with sea buckthorn at the rate of 2 and 3 g/kg improved the egg quality parameters and egg cholesterol.

• Human Ecology Research
• /
• 제53권1호
• /
• pp.17-27
• /
• 2015

This study was performed to investigate the antioxidative properties of sea buckthorn (Hippophae rhamnoides L.; leaf, fruit and stem) and the quality characteristics of brown rice sulgidduk prepared using a powder of sea buckthorn leaves. First, the antioxidative activities of sea buckthorn were measured to choose the most effective part of this plant. By analyzing the measured values, we concluded that the effective part of sea buckthorn was its leaves. The $IC_{50}$ value of the DPPH radical scavenging activity and the ABTS radical scavenging activity in sea buckthorn leaves were $7.78{\mu}g/mL$ and $264.04{\mu}g/mL$, respectively. The total polyphenol and total flavonoid contents of sea buckthorn leaves were 3.80 mg/mL and .19 mg/mL, respectively. Therefore, the brown rice sulgidduk was prepared using a powder of sea buckthorn leaves in the weight ratio of 0%, .5%, 1.0%, 1.5%, 2.0% and 2.5%. For analyzing the quality characteristics of the prepared sulgidduk, proximate compositions, color and texture profiles were measured and a sensory evaluation was conducted. With an increase in the added content of the sea buckthorn leaf powder (SBLP), the L-value significantly decreased while the a-value and the b-value increased. In the case of texture profiles, the control group (control A) had a higher score for hardness than the case groups in which the SBLP was added. However, springiness, chewiness and adhesiveness were not significantly different among the groups. In the sensory evaluation, the sample containing 1.5% SBLP yielded the best results. Therefore, we suggest that the powder of sea buckthorn leaves is a good ingredient for increasing the consumer acceptability and functionality of sulgidduk.

• 한방비만학회지
• /
• 제15권2호
• /
• pp.93-103
• /
• 2015

Objectives: It was investigated the cytoprotective efficacies of isorhamnetin, a flavonoid originally derived from Hippophae rhamnoides L., against oxidative stress-induced apoptosis in C2C12 myoblasts. Methods: The effects of isorhamnetin on cell growth, apoptosis and reactive oxygen species (ROS) generation were evaluated by trypan blue dye exclusion assay, 4',6-diamidino-2-phenylindole staining and flow cytometry. The levels of apoptosis-regulatory and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway-related proteins, and caspase activities (caspase-3 and -9) were determined by Western blot analysis and colorimetric assay, respectively. Results: Our results revealed that treatment with isorhamnetin prior to hydrogen peroxide ($H_2O_2$) exposure significantly increased the C2C12 cell viability and, indicating that the exposure of C2C12 cells to isorhamnetin conferred a protective effect against oxidative stress. Isorhamnetin also effectively attenuated $H_2O_2$-induced apoptosis and ROS generation, which was associated with the restoration of the upregulation of Bax and downregulation of Bcl-2 induced by $H_2O_2$. In addition, $H_2O_2$ enhanced the activation of caspase-9 and -3, and degradation of poly (ADP-ribose)-polymerase, a typical substrate protein of activated caspase-3; however, these events were almost totally reversed by pretreatment with isorhamnetin. Moreover, isorhamnetin increased the levels of heme oxygenase-1, a potent antioxidant enzyme, associated with the induction of Nrf2. Conclusions: Our data indicated that isorhamnetin may potentially serve as an agent for the treatment and prevention of muscle disorders caused by oxidative stress.

• Journal of Microbiology and Biotechnology
• /
• 제20권12호
• /
• pp.1724-1734
• /
• 2010

A phosphate-solubilizing bacterial strain isolated from Hippophae rhamnoides rhizosphere was identified as Rahnella sp. based on its phenotypic features and 16S rRNA gene sequence. The bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of inorganic and organic phosphate solubilization, 1-aminocyclopropane-1-carboxylate-deaminase activity, ammonia generation, and siderophore production. The strain also produced indole-3-acetic acid, indole-3-acetaldehyde, indole-3-acetamide, indole-3-acetonitrile, indole-3-lactic acid, and indole-3-pyruvic acid in tryptophan-supplemented nutrient broth. Gluconic, citric and isocitric acids were the major organic acids detected during tricalcium phosphate solubilization. A rifampicin-resistant mutant of the strain exhibited high rhizosphere competence without disturbance to the resident microbial populations in pea rhizosphere. Seed bacterization with a charcoal-based inoculum significantly increased growth in barley, chickpea, pea, and maize under the controlled environment. Microplot testing of the inoculum at two different locations in pea also showed significant increase in growth and yield. The attributes of cold-tolerance, high rhizosphere competence, and broad-spectrum plant growth-promoting activity exhibited the potential of Rahnella sp. BIHB 783 for increasing agriculture productivity.

• 한국식품영양과학회지
• /
• 제44권1호
• /
• pp.35-43
• /
• 2015

본 연구에서는 노루궁뎅이 버섯균사체를 비타민나무 잎에 배양하여 조제한 노루궁뎅이 버섯균사체-비타민나무 발효물 열수 추출물이 생체고분자의 산화적 손상과 세포사멸을 보호할 수 있는지를 관찰하였다. 노루궁뎅이 버섯균사체-비타민나무 발효물의 항산화 활성을 DPPH radical, ABTS radical, peroxyl radical 소거활성 측정을 통해 알아보았다. 그 결과 노루궁뎅이 버섯균사체-비타민나무 발효물을 처리한 DPPH radical 소거활성은 $500{\mu}g/mL$ 농도에서 65.06%, ABTS radical 소거활성은 $50{\mu}g/mL$ 농도에서 98.83%, peroxyl radical 소거활성은 $100{\mu}g/mL$ 농도에서 44.03%로 높은 항산화 활성을 나타내었다. 노루궁뎅이 버섯균사체-비타민나무 발효물은 DNA의 산화적 손상을 효과적으로 억제하였다. 노루궁뎅이 버섯균사체-비타민나무 발효물 역시 사람의 혈청단백질과 Cu,Zn-SOD의 산화적 손상을 억제하였다. 세포에 $H_2O_2$를 처리하였을 때 세포생존율에 비하여 발효물을 $100{\mu}g/mL$ 농도로 전 처리한 세포생존율은 21.59% 높게 증가되었다. 또한 발효물을 $50{\mu}g/mL$ 농도로 처리했을 경우 세포 내 ROS의 축적이 유의적으로 감소되었다. 따라서 노루궁뎅이 버섯균사체-비타민나무 발효물은 항산화 활성뿐만 아니라 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용이 뛰어난 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권7호
• /
• pp.3035-3042
• /
• 2015

Background: Isorhamnetin (Iso), a novel and essential monomer derived from total flavones of Hippophae rhamnoides that has long been used as a traditional Chinese medicine for angina pectoris and acute myocardial infarction, has also shown a spectrum of antitumor activity. However, little is known about the mechanisms of action Iso on cancer cells. Objectives: To investigate the effects of Iso on A549 lung cancer cells and underlying mechanisms. Materials and Methods: A549 cells were treated with $10{\sim}320{\mu}g/ml$ Iso. Their morphological and cellular characteristics were assessed by light and electronic microscopy. Growth inhibition was analyzed by MTT, clonogenic and growth curve assays. Apoptotic characteristics of cells were determined by flow cytometry (FCM), DNA fragmentation, single cell gel electrophoresis (comet) assay, immunocytochemistry and terminal deoxynucleotidyl transferase nick end labeling (TUNEL). Tumor models were setup by transplanting Lewis lung carcinoma cells into C57BL/6 mice, and the weights and sizes of tumors were measured. Results: Iso markedly inhibited the growth of A549 cells with induction of apoptotic changes. Iso at $20{\mu}g/ml$, could induce A549 cell apoptosis, up-regulate the expression of apoptosis genes Bax, Caspase-3 and P53, and down-regulate the expression of Bcl-2, cyclinD1 and PCNA protein. The tumors in tumor-bearing mice treated with Iso were significantly smaller than in the control group. The results of apoptosis-related genes, PCNA, cyclinD1 and other protein expression levels of transplanted Lewis cells were the same as those of A549 cells in vitro. Conclusions: Iso, a natural single compound isolated from total flavones, has antiproliferative activity against lung cancer in vitro and in vivo. Its mechanisms of action may involve apoptosis of cells induced by down-regulation of oncogenes and up-regulation of apoptotic genes.

• 한국환경생태학회지
• /
• 제28권2호
• /
• pp.263-269
• /
• 2014

본 연구는 사막화가 진행 중인 몽골의 엘센타사라이 지역을 중심으로, 주민 설문조사, 조림지 현장답사, 관련 문헌고찰 등을 통해 환경생태적 및 경제적 효과를 동시에 달성할 산림농업의 전략을 모색하였다. 산림농업의 접근유형은 사막화 및 작물 풍해를 방지하기 위한 방풍조림, 소득자원 생산을 위한 농경, 대상지역의 주 산업인 축산 등을 함께 배합하는 농림축혼업을 제안하였다. 주민의견과 생육환경을 반영하여, 조림수종은 사막지대의 자생종인 시베리아포플러와 비술나무를, 경제작목은 짧은 생장기간에 재배 및 수확이 가능한 갈매보리수, 감자, 사료작물 등을 각각 선정하였다. 농림축혼업의 다목적 효과를 충족시킬 토지배분 전략으로서, 다열의 방풍용 수목과 소득용 작물을 교호 대상으로 배식하는 간작기법을 권장하였다. 아울러 강풍과 건조에 대응하여, 경제작목 생산 및 수목생장의 조건을 함께 개선하기 위한 간작 시스템 관련 바람직한 식재기법을 모색하였다. 본 결과는 관련 연구가 미흡한 몽골은 물론 유사한 환경의 반건조지역에서, 지속가능한 산림농업을 추진하기 위한 유용한 기반정보가 될 것으로 기대한다.