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http://dx.doi.org/10.3746/jkfn.2015.44.1.035

Protective Effects of Sea Buckthorn (Hippophae rhamnoides L.) Leaves Fermented with Hericium erinaceum Mycelium against Oxidative Modification of Biological Macromolecules and Cell Death  

Kim, Seung-Sub (Department of Biomedical Science, Cheongju University)
Kyeong, Inn-Goo (R&D Center, Cosis Bio Corporation Limited)
Lee, Mi-La (R&D Center, Cosis Bio Corporation Limited)
Kim, Dong-Goo (R&D Center, Cosis Bio Corporation Limited)
Shin, Ji-Young (R&D Center, Cosis Bio Corporation Limited)
Yang, Jin-Yi (Department of Biomedical Science, Cheongju University)
Lee, Gwang-Ho (Department of Biomedical Science, Cheongju University)
Eum, Won-Sik (Research Institute of Bioscience and Biotechnology, Hallym University)
Kang, Jung-Hoon (Department of Biomedical Science, Cheongju University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.1, 2015 , pp. 35-43 More about this Journal
Abstract
In this study, hot water extract from sea buckthorn (Hippophae rhamnoides L.) leaves fermented with Hericium erinaceum mycelium (SBT-HE) was assessed for protection against oxidative modification of biological macromolecules and cell death. Antioxidant activity of SBT-HE was evaluated based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, and peroxyl radical scavenging assays. SBT-HE showed 65.06% DPPH radical scavenging activity at $500{\mu}g/mL$, 98.83% ABTS radical scavenging activity at $50{\mu}g/mL$, and 44.03% peroxyl radical scavenging activity at $100{\mu}g/mL$. SBT-HE significantly inhibited DNA strand breakage induced by peroxyl radical. SBT-HE also prevented peroxyl radical-mediated human serum albumin modification. SBT-HE effectively inhibited $H_2O_2$-induced cell death and significantly increased cell survival by 21.59% at $100{\mu}g/mL$. SBT-HE also reduced intracellular reactive oxygen species levels in $H_2O_2$-treated cells. The results suggest that SBT-HE can contribute to antioxidant activity and protect cells from oxidative stress-induced cell injury.
Keywords
oxidative modification; biological macromolecules; antioxidant activity; reactive oxygen species;
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