• The Korean Journal of Malacology
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• v.32 no.2
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• pp.119-125
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• 2016

The present study aimed to understand the effects of a ${\beta}$-glucan-added abalone feed (Immuneglu$^{TM}$) on the immune parameters, survival rate, and anti-bacterial activity of the abalone Nordotis discus hannai. During the study, spat and adult abalones were fed 0, 0.1, and 1% of Immuneglu$^{TM}$ mixed with artificial feed for 2 weeks, and their immune parameters, survival rate, and anti-bacterial activity were measured. The results showed that abalones fed on Immuneglu$^{TM}$-added feed showed a higher phagocytic rate, survival rate, and anti-bacterial activity against Vibrio alginolyticus, than those with the control treatment. In addition, the nitric oxide concentration, which indicates an inflammatory response, increased in the treatment group. Our study suggests that Immuneglu$^{TM}$ might be a useful additive for increasing the immunity and survival rates of abalones during mass culture.

• The Korean Journal of Physiology and Pharmacology
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• v.10 no.3
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• pp.155-159
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• 2006

Among the Shc proteins, p66shc is known to be related to oxidative stress responses and regulation of the production of reactive oxygen species (ROS). The present study was undertaken to investigate the role of p66shc on endothelial nitric oxide synthase (eNOS) activity in the mouse embryonic fibroblasts (MEFs). When wild type (WT) or p66shc (-/-) MEFs were transfected with full length of eNOS cDNA, the expression and activity of eNOS protein were higher in the p66shc (-/-) MEFs. These phenomena were reversed by reconstitution of p66shc cDNA transfection in the p66shc (-/-) MEFs. The basal superoxide production in the p66shc (-/-) MEFs was not significantly different from that of WT of MEFs. However, superoxide production induced by NADPH in the p66shc (-/-) MEF was lesser than that in WT MEFs. When compared with WT MEFs, cell lysate of p66shc (-/-) MEFs showed significantly increased H-ras activity without change of endogenous H-ras expression. Our findings suggest the pivotal role of p66shc adaptor protein played in inhibition of endothelial nitric oxide production via modulation of the expression and/or activity of eNOS protein.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.3
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• pp.269-278
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• 2014

Various antiepileptic drugs (AEDs) especially enzyme-inducing AEDs might be associated with increased vascular risk, through impairment of the endogenous antioxidative ability which may trigger oxygen-dependent tissue injury. Lamotrigine (LTG) a non-enzyme-inducing AED has scarce information regarding its effects on oxidative stress. The present study aimed to study the possible modulation of vascular risk factors of epileptogenesis by LTG, in a rat model of kindling seizure induced by pentylenetetrazole (PTZ). Four groups of male Wister rats were used; vehicle control group, PTZ group (alternate day PTZ, 30 mg/kg, i.p), LTG/PTZ group (LTG 20 mg/kg/day p.o and alternate day PTZ) and LTG group. The study period was 5 weeks. Lipoproteins and total homocysteine (tHcy), malondialdehyde (MDA) and reduced glutathione (GSH) were measured. Aortic endothelial function study and histopathological examination of the rats' brains, aortas and coronaries were conducted. Serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), tHcy, MDA, GSH levels were significantly higher in epileptic rats than normal controls rats. A decrease in HDL-cholesterol with high atherosclerotic index was also demonstrated. The administration of LTG improved the PTZ-kindled seizures. It produced a significant decrease in TC, TG and LDL-cholesterol, MDA, aortic GSH and increase in HDL-cholesterol with no significant effect on serum GSH and tHcy levels. LTG improved endothelium-dependent relaxation, decreased hippocampal neurodegenerative changes and atherosclerotic changes of aortas and coronaries. LTG decreased seizures severity, hippocampal damage and improved vascular risk markers in this rat model of kindling seizures.

• Nutrition Research and Practice
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• v.9 no.4
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• pp.343-349
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• 2015

BACKGROUND/OBJECTIVES: Fermentation of dietary fiber results in production of various short chain fatty acids in the colon. In particular, butyrate is reported to regulate the physical and functional integrity of the normal colonic mucosa by altering mucin gene expression or the number of goblet cells. The objective of this study was to investigate whether butyrate modulates mucin secretion in LS174T human colorectal cells, thereby influencing the adhesion of probiotics such as Lactobacillus and Bifidobacterium strains and subsequently inhibiting pathogenic bacteria such as E. coli. In addition, possible signaling pathways involved in mucin gene regulation induced by butyrate treatment were also investigated. MATERIALS/METHODS: Mucin protein content assay and periodic acid-Schiff (PAS) staining were performed in LS174T cells treated with butyrate at various concentrations. Effects of butyrate on the ability of probiotics to adhere to LS174T cells and their competition with E. coli strains were examined. Real time polymerase chain reaction for mucin gene expression and Taqman array 96-well fast plate-based pathway analysis were performed on butyrate-treated LS174T cells. RESULTS: Treatment with butyrate resulted in a dose-dependent increase in mucin protein contents in LS174T cells with peak effects at 6 or 9 mM, which was further confirmed by PAS staining. Increase in mucin protein contents resulted in elevated adherence of probiotics, which subsequently reduced the adherent ability of E. coli. Treatment with butyrate also increased transcriptional levels of MUC3, MUC4, and MUC12, which was accompanied by higher gene expressions of signaling kinases and transcription factors involved in mitogen-activated protein kinase (MAPK) signaling pathways. CONCLUSIONS: Based on our results, butyrate is an effective regulator of modulation of mucin protein production at the transcriptional and translational levels, resulting in changes in the adherence of gut microflora. Butyrate potentially stimulates the MAPK signaling pathway in intestinal cells, which is positively correlated with gut defense.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.259-264
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• 2005

Transplantation of cultured chondrocytes can regenerate cartilage tissues in cartilage defects in humans. However, this method requires a long culture period to expand chondrocytes to a large number of cells for transplantation. In addition, chondrocytes may dedifferentiate during long-term culture. These problems can potentially be overcome by the use of undifferentiated or partially developed cartilage precursor cells derived from neonatal cartilage, which, unlike chondrocytes from adult cartilage, have the capacity for rapid in vitro cell expansion and may retain their differentiated phenotype during long-term culture. The purpose of this study was to compare the cell growth rate and phenotypic modulation during in vitro culture between adult chondrocytes and neonatal chondrocytes, and to demonstrate the feasibility of regenerating cartilage tissues in vivo by transplantation of neonatal chondrocytes expanded in vitro and seeded onto polymer scaffolds. When cultured in vitro, chondrocytes isolated from neonatal (immediately postpartum, 2 h of age) rats exhibited much higher growth rate than chondrocytes isolated from adult rats. After 5 days of culture, more neonatal chondrocytes were in the differentiated state than adult chondrocytes. Cultured neonatal chondrocytes were seeded onto biodegradable polymer scaffolds and transplanted into athymic mice's subcutaneous sites. Four weeks after implantation, neonatal chondrocyte-seeded scaffolds formed white cartilaginous tissues. Histological analysis of the implants with hematoxylin and eosin showed mature and well-formed cartilage. Alcian blue/ safranin-O staining and Masson's trichrome staining indicated the presence of highly sulfated glycosarninoglycans and collagen, respectively, both of which are the major extracellular matrices of cartilage. Immunohistochemical analysis showed that the collagen was mainly type II, the major collagen type in cartilage. These results showed that neonatal chondrocytes have potential to be a cell source for cartilage tissue engineering.

• Journal of Korea Multimedia Society
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• v.14 no.11
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• pp.1431-1437
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• 2011

This paper proposes a novel watermarking algorithm that protects digital copyrights and is robust to attacks. Watermarks are embedded in the subband including the significant part of the signal such as a pitch. Generally, the subband containing the pitch has the biggest energy. In order to find this subband, wavelet packet transform is used to decompose the subbands and their energy are calculated. The signal of the selected subbands is transformed in frequency domain using FFT. The watermarks are embedded using QIM for samples higher than a certain threshold. The blind detection uses the Euclidean distance. The proposed method shows less than 5% BER in the audio watermark benchmarking.

• Journal of Korean Society of Archives and Records Management
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• v.12 no.1
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• pp.189-207
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• 2012

It is necessary to diagnose the standard record management system when the system is being spread out to entire public organization and institutions. This study aims to analyze the standard record management system for improving system and revising the related standards in the future. This study evaluates the satisfaction on the interface and functional quality of the system through questionnaire survey on records managers. It is found that the satisfaction of functional quality is just above the line, but the interface fails to satisfy records managers. Also the reliability of the system appears to be higher satisfaction than efficiency and usability. Therefore, the study suggests that the standard records management systems should try to improve 'easy of use' and effectiveness and efficiency by implementing heavy batch job and promoting modulation of key function. And the study emphasizes that the standards will be revised with the long-term plan in order to develop standard records management system systematically and continuously.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.24 no.4
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• pp.417-425
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• 2013

Digital communication technology have been focused on the higher data efficiency and the effective coding of information. Also, it is very important to improve security and minimize the interference of the external sources, Chaos communications system is studied to improve security and minimize the interference by effective encoding of information in the nonlinear systems such as DCSK(Differential Chaos Shift Keying) and CDSK(Correlation Delay Shift Keying). In this paper, we evaluate anti-jamming performance of chaos communications system using the DCSK and CDSK system. The simulation results show that the Gauss iterated map has better BER performance than the other maps(tent map, logistic map, Henon map). Also, DCSK and CDSK system can get the better anti-jamming performance by applying different chaotic maps.

• Journal of Broadcast Engineering
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• v.17 no.5
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• pp.864-875
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• 2012

In this paper, we propose a carrier frequency offset estimation method for single-carrier MIMO systems. In the proposed method, phase rotated PN (Pseudo-Noise) sequences are transmitted to prevent a cancelling out of partial PN sequences. After removing a modulation of received PN sequences by multiplying of complex conjugated PN Sequences which are locally generated in receiver, a CFO (Carrier Frequency Offset) is accurately estimated by employing L&R method which is a kind of ML (Maximum Likelihood) estimation algorithm and uses multiple auto-correlatos. In addition, the frequency offset estimation scheme by using channel state information is proposed for accurate CFO estimation in time-varying Rayleigh channel. By performing computer simulations, MSE (Mean Square Error) performance of proposed method is almost same as MSE performance of SISO systems in AWGN channel. Moreover, MSE Performance of proposed method with using channel information is higher than MSE performances of SISO system and conventional method in time-varying Rayleigh channel.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2002.09a
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• pp.385-388
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• 2002

We measured and evaluated digital, pre-sampling and overall imaging properties (characteristic curves, Modulation Transfer Function (MTF), Wiener spectra (WS), Noise Equivalent Quanta (NEQ) and Detective Quantum Efficiency (DQE)) for the direct type and indirect type of Flat-Panel Detector (FPD). First, the digital and overall characteristic curves of the both types of FPD had more wide dynamic range than that of the S/F system. Second, the pre-sampling and overall MTF of the direct-type FPD were superior to those of the indirect-type FPD. Third, for identical exposures, the digital and overall WS of the direct-type FPD were similar or worse than those of the indirect-type FPD, and for larger exposure, the digital WS of the both types of FPD were smaller, but overall WS of the both types of FPD were larger. Fourth, the digital and overall NEQ and DQE of the direct-type FPD were worse than both NEQ and DQE of the indirect-type FPD at lower spatial frequencies, but were better at higher spatial frequencies.