• 제목/요약/키워드: High-throughput screen

검색결과 32건 처리시간 0.022초

Identification of Novel Regulators of Apoptosis Using a High-Throughput Cell-based Screen

  • Park, Kyung Mi;Kang, Eunju;Jeon, Yeo-Jin;Kim, Nayoung;Kim, Nam-Soon;Yoo, Hyang-Sook;Yeom, Young Il;Kim, Soo Jung
    • Molecules and Cells
    • /
    • 제23권2호
    • /
    • pp.170-174
    • /
    • 2007
  • High-throughput subcellular imaging is a powerful tool for investigating the function of genes. In order to identify novel regulators of apoptosis we transiently transfected HeLa cells with 938 hypothetical genes of unknown function, and captured their nuclear images with an automated fluorescence microscope. We selected genes that induced greater than 3-fold increase in the percentage of apoptotic nuclei compared with vector-transfected cells. The full-length genes C10orf61, MGC 26717, and FLJ13855 were identified as candidate proapoptotic genes, and their apoptotic effects were confirmed by DNA fragmentation ELISAs and Western blotting for caspase-7 and PARP. We conclude that a subcellular image-based apoptotic screen is useful for identifying genes with proapoptotic activity.

OSMU N-스크린 서비스를 위한 WiMedia D-MAC에서 멀티캐스트 릴레이 전송 기술의 성능 분석 (Performance Analysis of Multicast Relay Transmissions in WiMedia D-MAC for OSMU N-Screen Services)

  • 허경
    • 한국정보통신학회논문지
    • /
    • 제20권12호
    • /
    • pp.2267-2273
    • /
    • 2016
  • 본 논문에서는 OSMU (One Source Multi Use) N-스크린 멀티캐스트 서비스를 위한 무선 통신 MAC 구조로서, WiMedia Distributed-MAC 프로토콜을 적용하였다. 그러나, 채널 에러율이 가변적인 무선 통신환경을 고려하면, N-스크린 고속 데이터가 손실될 가능성이 높다고 할 수 있다. 이러한 문제에 대해, WiMedia Distributed-MAC 프로토콜을 분석하여, 멀티캐스트 릴레이 기술을 제안하였다. 제안하는 멀티캐스트 릴레이 기술은 Multicast-free DRP Availability IE 기술과 결합되어, 멀티캐스트 통신에 대해 릴레이노드를 선정하고 에러율이 높은 채널을 회피하여 통신할 수 있다. 시뮬레이션을 통해 제안하는 멀티캐스트 릴레이 기술과 기존 WiMedia Distributed-MAC 멀티캐스트 기술을 멀티캐스트 노드 수에 따라 수율과 에너지 소모량을 비교 하였고, 다양한 BER (Bit Error Rate) 채널 환경에서 수율 성능을 비교하였다. 이를 통해, OSMU N-스크린 멀티캐스트 서비스에서 멀티캐스트 릴레이 기술이 적용되어야 함을 설명하였다.

유전체 코호트 연구를 위한 대용량 염기서열 분석 (High Throughput Genotyping for Genomic Cohort Study)

  • 박웅양
    • Journal of Preventive Medicine and Public Health
    • /
    • 제40권2호
    • /
    • pp.102-107
    • /
    • 2007
  • Human Genome Project (HGP) could unveil the secrets of human being by a long script of genetic codes, which enabled us to get access to mine the cause of diseases more efficiently. Two wheels for HGP, bioinformatics and high throughput technology are essential techniques for the genomic medicine. While microarray platforms are still evolving, we can screen more than 500,000 genotypes at once. Even we can sequence the whole genome of an organism within a day. Because the future medicne will focus on the genetic susceptibility of individuals, we need to find genetic variations of each person by efficient genotyping methods.

혈장 시료 풀링을 통한 신약 후보물질의 흡수율 고효율 검색기법의 평가 (Evaluation of a Sample-Pooling Technique in Estimating Bioavailability of a Compound for High-Throughput Lead Optimazation)

  • 이인경;구효정;정석재;이민화;심창구
    • Journal of Pharmaceutical Investigation
    • /
    • 제30권3호
    • /
    • pp.191-199
    • /
    • 2000
  • Genomics is providing targets faster than we can validate them and combinatorial chemistry is providing new chemical entities faster than we can screen them. Historically, the drug discovery cascade has been established as a sequential process initiated with a potency screening against a selected biological target. In this sequential process, pharmacokinetics was often regarded as a low-throughput activity. Typically, limited pharmacokinetics studies would be conducted prior to acceptance of a compound for safety evaluation and, as a result, compounds often failed to reach a clinical testing due to unfavorable pharmacokinetic characteristics. A new paradigm in drug discovery has emerged in which the entire sample collection is rapidly screened using robotized high-throughput assays at the outset of the program. Higher-throughput pharmacokinetics (HTPK) is being achieved through introduction of new techniques, including automation for sample preparation and new experimental approaches. A number of in vitro and in vivo methods are being developed for the HTPK. In vitro studies, in which many cell lines are used to screen absorption and metabolism, are generally faster than in vivo screening, and, in this sense, in vitro screening is often considered as a real HTPK. Despite the elegance of the in vitro models, however, in vivo screenings are always essential for the final confirmation. Among these in vivo methods, cassette dosing technique, is believed the methods that is applicable in the screening of pharmacokinetics of many compounds at a time. The widespread use of liquid chromatography (LC) interfaced to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) allowed the feasibility of the cassette dosing technique. Another approach to increase the throughput of in vivo screening of pharmacokinetics is to reduce the number of sample analysis. Two common approaches are used for this purpose. First, samples from identical study designs but that contain different drug candidate can be pooled to produce single set of samples, thus, reducing sample to be analyzed. Second, for a single test compound, serial plasma samples can be pooled to produce a single composite sample for analysis. In this review, we validated the issue whether the second method can be applied to practical screening of in vivo pharmacokinetics using data from seven of our previous bioequivalence studies. For a given drug, equally spaced serial plasma samples were pooled to achieve a 'Pooled Concentration' for the drug. An area under the plasma drug concentration-time curve (AUC) was then calculated theoretically using the pooled concentration and the predicted AUC value was statistically compared with the traditionally calculated AUC value. The comparison revealed that the sample pooling method generated reasonably accurate AUC values when compared with those obtained by the traditional approach. It is especially noteworthy that the accuracy was obtained by the analysis of only one sample instead of analyses of a number of samples that necessitates a significant man-power and time. Thus, we propose the sample pooling method as an alternative to in vivo pharmacokinetic approach in the selection potential lead(s) from combinatorial libraries.

  • PDF

Gravure off-set 인쇄법을 적용한 고효율 다결정 실리콘 태양전지 (Gravure off-set printing method for the high-efficiency multicrystalline-silicon solar cell)

  • 김동주;김정모;배소익;전태현;송하철
    • 한국태양에너지학회:학술대회논문집
    • /
    • 한국태양에너지학회 2011년도 춘계학술발표대회 논문집
    • /
    • pp.293-298
    • /
    • 2011
  • The most widely used method to form an electrode in industrial solar cells are screen printing. Screen printing is characterized by a relatively simple and well-known production sequence with high throughput rates. However the method is difficult to implement a fine line width of high-efficiency solar cells can not be made. The open circuit voltage(Voc) and the short circuit current density(Jsc) and fill factor(FF) need to be further improved to increase the efficiency of silicon solar cells. In this study, gravure offset printing method using the multicrystalline-silicon solar cells were fabricated. Gravure off-set printing method which can print the fine line width of finger electrode can have the ability reduce the shaded area and increase the Jsc. Moreover it can make a high aspect ratio thereby series resistance is reduced and FF is increased. Approximately $50{\mu}m$ line width with $35{\mu}m$ height was achieved. The efficiency of gravure off set was 0.7% higher compare to that of scree printing method.

  • PDF

식품소재 라이브러리를 이용한 천식 완화용 물질의 초고속스크리닝 기법 개발 (Development of High Throughput Screening Techniques Using Food-borne Library against Anti-asthma Agents)

  • 허진철;박자영;권택규;정신교;김성욱;이상한
    • 한국식품저장유통학회지
    • /
    • 제12권3호
    • /
    • pp.267-274
    • /
    • 2005
  • 천식과 관련하여 산화스트레스 (Oxidant stress)는 그 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 농산물과 한약재를 이용하여 항산화 물질을 찾고자 하였으며, 시간과 비용의 단축을 위하여 HTS인 throughput screening)을 이용을 하였다. 항산화 실험과 관련하여서 DPPH(1,1-diphenyl-2-picrylhydrazyl), FRAP(ferric ion reducing antioxidant power), HO(hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 시행하였다. 이후 $H_{2}O_2$에 의한 산화스트레스를 이용한 세포사멸을 유도하여 세포생존을 확인해 보았다. 실험결과 해바라기씨(Helianthus annuus), 신선초(Angelica utilis Makino), 시금치(Rehmannia glutinosa Libo) 등이 활성이 높게 나타났다. 본 연구는 여기에서 나온 hit를 이용하여 동물모델 실험을 진행하고자 한다.

Nanoliter Reactor Arrays for Antibiotic Study

  • Park, Jin-Won
    • Bulletin of the Korean Chemical Society
    • /
    • 제28권10호
    • /
    • pp.1709-1714
    • /
    • 2007
  • It is demonstrated in this study that the nanoliter reactor arrays with an inkjet printing, can be used for high throughput screen of antibiotic function. As a model antibiotic, gramicidin was used in this study. The gramicidin embedded lipid vesicles were immobilized on the surface in the nanoliter reactor structure with control of the volume in the nanoliter reactor. By dispensing acidic drops into the reactor, the gramicidin function was monitored. The technique developed in this research also has a great potential to be used for discovery of drugs.