• Title/Summary/Keyword: High-throughput analysis

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High-throughput identification of chrysanthemum gene function and expression: An overview and an effective proposition

  • Nguyen, Toan Khac;Lim, Jin Hee
    • Journal of Plant Biotechnology
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    • v.48 no.3
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    • pp.139-147
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    • 2021
  • Since whole-genome duplication (WGD) of diploid Chrysanthemum nankingense and de novo assembly whole-genome of C. seticuspe have been obtained, they have afforded to perceive the diversity evolution and gene discovery in the improved investigation of chrysanthemum breeding. The robust tools of high-throughput identification and analysis of gene function and expression produce their vast importance in chrysanthemum genomics. However, the gigantic genome size and heterozygosity are also mentioned as the major obstacles preventing the chrysanthemum breeding practices and functional genomics analysis. Nonetheless, some of technological contemporaries provide scientific efficient and promising solutions to diminish the drawbacks and investigate the high proficient methods for generous phenotyping data obtaining and system progress in future perspectives. This review provides valuable strategies for a broad overview about the high-throughput identification, and molecular analysis of gene function and expression in chrysanthemum. We also contribute the efficient proposition about specific protocols for considering chrysanthemum genes. In further perspective, the proper high-throughput identification will continue to advance rapidly and advertise the next generation in chrysanthemum breeding.

High Throughput Radix-4 SISO Decoding Architecture with Reduced Memory Requirement

  • Byun, Wooseok;Kim, Hyeji;Kim, Ji-Hoon
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.14 no.4
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    • pp.407-418
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    • 2014
  • As the high-throughput requirement in the next generation communication system increases, it becomes essential to implement high-throughput SISO (Soft-Input Soft-Output) decoder with minimal hardware resources. In this paper, we present the comparison results between cascaded radix-4 ACS (Add-Compare-Select) and LUT (Look-Up Table)-based radix-4 ACS in terms of delay, area, and power consumption. The hardware overhead incurred from the retiming technique used for high speed radix-4 ACS operation is also analyzed. According to the various analysis results, high-throughput radix-4 SISO decoding architecture based on simple path metric recovery circuit is proposed to minimize the hardware resources. The proposed architecture is implemented in 65 nm CMOS process and memory requirement and power consumption can be reduced up to 78% and 32%, respectively, while achieving high-throughput requirement.

Performance Analysis of LTE-R Radio Communication Systems in High Speed Train Environment (열차 고속 주행환경에서 LTE-R 무선통신시스템 성능 분석)

  • Kwon, You-Chul;Choi, Jun-Sung;Oh, Hyun-Seo;Kim, Seong-Cheol
    • The Journal of the Korea institute of electronic communication sciences
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    • v.17 no.4
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    • pp.587-594
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    • 2022
  • This paper analyzed radio link throughput performance by applying radio link simulation and test equipment because there is limit in train filed test in 420 km/h high speed environment. According to the radio performance analysis, the throughput performance is slightly degraded in QPSK and 16QAM modulation schemes. But throughput performance is highly degraded in 64QAM modulation scheme.

Throughput of Coded DS CDMA/Unslotted ALOHA Networks with Variable Length Data Traffic and Two User Classes in Rayleigh Fading FSMC Model

  • Tseng, Shu-Ming;Chiang, Li-Hsin;Wang, Yung-Chung
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.8 no.12
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    • pp.4324-4342
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    • 2014
  • Previous papers analyzed the throughput performance of the CDMA ALOHA system in Rayleigh fading channel, but they assume that the channel coefficient of Rayleigh fading was the same in the whole packet, which is not realistic. We recently proposed the finite-state Markov channel (FSMC) model to the throughput analysis of DS uncoded CDMA/unslotted ALOHA networks for fixed length data traffic in the mobile environment. We now propose the FSMC model to the throughput analysis of coded DS CDMA/unslotted ALOHA networks with variable length data traffic and one or two user classes in the mobile environment. The proposed DS CDMA/unslotted ALOHA wireless networks for two user classes with access control can maintain maximum throughput for the high priority user class under high message arrival per packet duration.

High-Throughput DNA Extraction Method for Marker Analysis in Rice Grain (대량의 쌀 시료 분석을 위한 DNA 추출법)

  • Choi, Young-Deok;Lee, Hae-Kwang;Lee, Yun-Suk;Yun, Jeong-Hee;Kim, Su-Jeong;Park, Seong-Whan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.269-273
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    • 2006
  • The study of molecular markers to improve crops largely depends on the availability of rapid and of efficient DNA extraction methods. Here we developed a cheap and convenient method to isolate genomic DNA from rice grains suitable for large-scale microsatellite analysis. We confirmed that the isolated rice DNA is suitable for PCR analysis with STS marker and SNP marker, as well as microsatellite marker. Further, we established high-throughput DNA extraction system in a 96-well plate format which make it possible high-throughput analysis of microsatellite markers with rice grains. This implies that the new method could be a useful tool for other types of marker analysis in large scale.

An Analysis of Effective Throughput in Distributed Wireless Scheduling

  • Radwan, Amr
    • Journal of Korea Multimedia Society
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    • v.19 no.2
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    • pp.155-162
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    • 2016
  • Several distributed scheduling policies have been proposed with the objective of attaining the maximum throughput region or a guaranteed fraction throughput region. These policies consider only the theoretical throughput and do not account the lost in throughput due to the time complexity of implementing an algorithm in practice. Therefore, we propose a novel concept called effective throughput to characterize the actual throughput by taking into account the time complexity. Effective throughput can be viewed as the actual transmitted data without including the control message overhead. Numerical results demonstrate that in practical scheduling, time complexity significantly affects throughput. The performance of throughput degrades when the time complexity is high.

Design on Main Mechanism of High Throughput Device for Enhancement of Oxidation and Recover Rate (산화속도 및 회수율 향상을 위한 고효율 장치 핵심 메커니즘 설계)

  • Kim, Y.H.;Park, B.S.;Jung, J.H.;Yoon, J.S.;Hwang, J.S.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2008.05a
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    • pp.473-476
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    • 2008
  • In this paper, we designed the main mechanism of high throughput device for rod-cuts of spent fuel. For this, we analyzed the mechanical methods(slitting, ball mill, roller straightening) and chemical methods(muffle furnace, rotary kiln). As the results, methods of ball drop and rotary drum for concepts design were selected in the analysis step. For enhancement of oxidation rate, we devised the blades on the reactor with mesh type. Also, for enhancement of decladding rate, we designed ball size and rotation reactor with mesh type and devised the vacuum system for fission products. Mechanisms of oxidation and recovery can simultaneously handle the rod-cuts of spent fuel and independently recover. The results of mechanism design can be used for scale-up of high throughput device.

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Throughput and Delay of Single-Hop and Two-Hop Aeronautical Communication Networks

  • Wang, Yufeng;Erturk, Mustafa Cenk;Liu, Jinxing;Ra, In-ho;Sankar, Ravi;Morgera, Salvatore
    • Journal of Communications and Networks
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    • v.17 no.1
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    • pp.58-66
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    • 2015
  • Aeronautical communication networks (ACN) is an emerging concept in which aeronautical stations (AS) are considered as a part of multi-tier network for the future wireless communication system. An AS could be a commercial plane, helicopter, or any other low orbit station, i.e., Unmanned air vehicle, high altitude platform. The goal of ACN is to provide high throughput and cost effective communication network for aeronautical applications (i.e., Air traffic control (ATC), air traffic management (ATM) communications, and commercial in-flight Internet activities), and terrestrial networks by using aeronautical platforms as a backbone. In this paper, we investigate the issues about connectivity, throughput, and delay in ACN. First, topology of ACN is presented as a simple mobile ad hoc network and connectivity analysis is provided. Then, by using information obtained from connectivity analysis, we investigate two communication models, i.e., single-hop and two-hop, in which each source AS is communicating with its destination AS with or without the help of intermediate relay AS, respectively. In our throughput analysis, we use the method of finding the maximum number of concurrent successful transmissions to derive ACN throughput upper bounds for the two communication models. We conclude that the two-hop model achieves greater throughput scaling than the single-hop model for ACN and multi-hop models cannot achieve better throughput scaling than two-hop model. Furthermore, since delay issue is more salient in two-hop communication, we characterize the delay performance and derive the closed-form average end-to-end delay for the two-hop model. Finally, computer simulations are performed and it is shown that ACN is robust in terms of throughput and delay performances.

Implementation of Z-Factor Statistics for Performance Evaluation of Quality Innovation in the High Throughput Process (High Throughput 프로세스에서 품질혁신의 성능평가를 위한 Z-Factor의 적용방안)

  • Choi, Sung-Woon
    • Journal of the Korea Safety Management & Science
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    • v.15 no.1
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    • pp.293-301
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    • 2013
  • The purpose of this study is to introduce the limit of previously used six sigma quality process evaluation metrics, $Z_{st}$ and $P_{pk}$, and a solution to overcome this drawback by using a metric based on performance evaluation of Z-factor quality innovation. Case analysis on projects from national six sigma contest from 2011 to 2012 is performed and literature review on new drug development HTS (High Throughput Screening) is used to propose innovative performance evaluation metrics. This research shows that experimental study on six sigma evaluation metric, $Z_{st}$ and $P_{pk}$, have no significance difference between industrial type (Manufacturing, Semi-Public Institute, Public Institute) and CTQ type (Product Technology Type CTQ, Process Technology Type CTQ). Following discovery characterize this quality improvement as fixed target type project. As newly developed moving target type of quality innovation performance metric Z-Factor is used for evaluating experimental study, hypothetical analysis suggests that $Z_{st}$ and $P_{pk}$ share different relationship or even show reciprocal relationship. Constraints of the study are relatively small sample size of only 37 projects from past 2 years and conflict on having interview and communication with six sigma quality practitioner for qualitative experimental study. Both moving target type six sigma innovation project and fixed target type improvement project or quality circle enables efficient ways for a better understanding and quality practitioner use by applying quality innovation performance metric. Downside of fixed target type quality performance evaluation metric, $Z_{st}$ and $P_{pk}$, is presented through experimental study. In contrast, advantage of this study is that high throughput requiring product technology, process technology and quantum leap typed innovation effect is evaluated based on precision and accuracy and Z-Factor that enables relative comparison between enterprises is proposed and implemented.

An Automated High Throughput Proteolysis and Desalting Platform for Quantitative Proteomic Analysis

  • Arul, Albert-Baskar;Han, Na-Young;Lee, Hookeun
    • Mass Spectrometry Letters
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    • v.4 no.2
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    • pp.25-29
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    • 2013
  • Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.