• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.253-261
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• 2007

Molecular and cultivation techniques were used to characterize the bacterial communities of biobead reactor biofilms in a sewage treatment plant to which an Aerated Up-Flow Biobead process was applied. With this biobead process, the monthly average values of various chemical parameters in the effluent were generally kept under the regulation limits of the effluent quality of the sewage treatment plant during the operation period. Most probable number (MPN) analysis revealed that the population of denitrifying bacteria was abundant in the biobead #1 reactor, denitrifying and nitrifying bacteria coexisted in the biobead #2 reactor, and nitrifying bacteria prevailed over denitrifying bacteria in the biobead #3 reactor. The results of the MPN test suggested that the biobead #2 reactor was a transition zone leading to acclimated nitrifying biofilms in the biobead #3 reactor. Phylogenetic analysis of 16S rDNA sequences cloned from biofilms showed that the biobead #1 reactor, which received a high organic loading rate, had much diverse microorganisms, whereas the biobead #2 and #3 reactors were dominated by the members of Proteobacteria. DGGE analysis with the ammonia monooxygenase (amoA) gene supported the observation from the MPN test that the biofilms of September were fully developed and specialized for nitrification in the biobead reactor #3. All of the DNA sequences of the amoA DGGE bands were very similar to the sequence of the amoA gene of Nitrosomonas species, the presence of which is typical in the biological aerated filters. The results of this study showed that organic and inorganic nutrients were efficiently removed by both denitrifying microbial populations in the anaerobic tank and heterotrophic and nitrifying bacterial biofilms well-formed in the three functional biobead reactors in the Aerated Up-Flow Biobead process.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.6
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• pp.323-328
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• 2016

Pressurized membrane used for side-stream MBR process requires fouling control strategy both for normal and abnormal operation conditions for stable operation of the facilities. In this study, $85m^3/day$ of pilot-scale side-stream MBR process was constructed for the evaluation of fouling mitigation by air bubble injection into the membrane module. In addition, fouling phenomena at abnormal operation conditions of low influent and/or loading rate were also investigated. Injection of air bubble was found to be effective in delaying transmembrane pressure (TMP) increase mainly due to scouring effect on the membrane surface, resulting in expanded filtration cycle at a high flux of $40L/m^2{\cdot}h$ (LMH). At abnormal operation condition, injection of PACl (53 mg/L as Al) into the bioreactor showed 19% reduction of TMP increase. However, inhibition of nitrifying bacteria by continuous PACl injection was observed from batch experiments. In contrast, injection of powdered activated carbon (PAC, 0.6 g/L) was able to maintain the initial TMP of $0.2kg/cm^2$ for 5 days at the abnormal conditions. It may have been caused from the adsorption of extracellular polymeric substances (EPS), which was known to be excessively released during growth inhibition condition and act as the major foulants in MBR operations.

• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.268-274
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• 2011

BACKGROUND: A large scale of sediment load delivered from watershed causes substantial waterway damages and water quality degradation. Controlling sediment loading requires the knowledge of the soil erosion and sedimentation. The various factors such as watershed size, slope, climate, land use may affect sediment delivery processes. Traditionally sediment delivery ratio prediction equations have been developed by relating watershed characteristics to measured sediment yield divided by predicted gross erosion. However, sediment prediction equations have been developed for only a few regions because of limited sediment data. Besides, little research has been done on the prediction of sediment delivery ratio for asia monsoon period in mountainous watershed. METHODS AND RESULTS: In this study Tank model was expanded and applied for estimating sediment yield to Oship River of east coast. The rainfall-runoff in 2006 was verified using the Tank model and we derived good result between observed and calculated discharge in 2009 at the same conditions. In relation to sediment yield, the sediment delivery rate of 2006 was very high than 2009 regardless of methods for estimating sediment load. It was thought to be affected by heavy rainfall due to the typhoon. CONCLUSION(s): For estimating sediment volume from watershed, long-term monitoring data on discharge and sediment is needed. This model will be able to apply to predict discharge and sediment yield simultaneously in ungauged area. This approach is more effective and less expensive method than the traditional method which needs a lot of data collection.

• Journal of the Society of Disaster Information
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• v.14 no.3
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• pp.379-386
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• 2018

Purpose: This study was to the fragility evaluation of I-shape curved beam structure subjected to strong ground motions including Gyeongju and Pohang earthquakes Method: In particular, to conduct the analytical model, ABAQUS and ANSYS platform was used in this study. Furthermore, the analytical model using 3D Finite Element Model (FEM) was validated, in comparison to the theoretical solutions at the location of 025L, 05L, and 0.75L in static loading condition. In addition, in order to evaluate the seismic fragility of the curved beam structure, 20 seismic ground motions were selected and Monte-Carlo Simulation was used for the empirical fragility evaluation from 0.2g to 1.5g. Result: It was interesting to find that the probability of the system failure was found at 0.2g, as using 190 MPa limit state and the probability of the failure using 390 MPa limit state was starting from 0.6g. Conclusion: This study showed the comparison of the theoretical solution with analytical solution on I-shaped curved beam structures and it was interesting to note that the system subjected to strong ground motions was sensitive to high frequency earthquake. Further, the seismic fragility corresponding to the curved beam shapes must be evaluated.

• Applied Biological Chemistry
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• v.39 no.5
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• pp.333-337
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• 1996

For continuous production of galactooligosaccharides(GOS), $\beta-galactosidase$ with h1gh transgalactosylation activity from Bacillus sp. A 4442 was Immobilized onto $Diaion^{TM}$ HPA 75(styrene-divinylbenzene resin). The parameters influencing enzyme immobilization were scrutinized in order to maximize immobilization yield while minimizing enzyme inactivation. The optimum conditions turned out to be: Tris buffer concentration 30 mM, pH 8.0, contact time at room temperature 3 hr, and enzyme loading 25 mg protein/g resin. Both the thermal stability and the operational stability of immobilized enzyme were markedly enchanced by the treatment with 0.5% glutaraldehyde as a cross-linker. Under the experimental conditions established, the yield of ${\beta}-galactosidase$ immobilization was 40% or more and the activity of the immobilized enzyme ca. 200 U/g resin. When a packed-bed reactor was employed to continuously convert lactose to GOS, the specific production, which refers to as the amount of commercially valuable GOS produced by a unit amount of immobilized ${\beta}-galactosidase$, was found to be ca. 300 g GOS/g carrier.

• Journal of the Korean Institute of Gas
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• v.7 no.2 s.19
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• pp.33-41
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• 2003

There exists high hazard when transporting LPG as well as using, storing, and producing. For small scale LPG consumer, retailers deliver LPG to customers via a truck loading many LPG cylinders. Suppose there occurred a accident during LPG cylinder transfer, this could result in serious damages to the life and properties in the near or neighbor of the accident spot. In this regard, we made a quantitative risk analysis to estimate the possible damages and the probability through the identification of accidents causes and the simulation of the possible scenario. In this study, we made the Excel & Visual Basic computer program to perform quantitative LPG accident analysis. The simulation showed the following results. In case of UVCE(Unconfined Vapor Cloud Explosion), the effect within l0m of the accident spot showed very severe structural damages and even the accident can break the window glasses of the area of 150 m apart from accident spot. In case of TNT corresponding probit analysis, after 10 minutes LPG leaking, $75\%$ window glasses of 40 m distance was expected to be broken. And $16\%$ frames of 20m distance, $10\%$ frames of 40m distance was expected to be collapsed.

• Journal of Korean Society of Steel Construction
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• v.20 no.1
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• pp.131-149
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• 2008

A transmission tower was designed according to general and special load cases based on KEPCO Design Specifications. The special load case such as unbalanced load a cording to some broken wires has not been considered significantly. Therefore, this paper presents investigations on the stability and safety of main post members subjected to unbalanced load and design wind load. In this study, all cases totally considered. From the finite element analyses using LUSAS program, the stresses on the tower subjected to unbalanced load and design wind load were very high in comparison to the allowable stresses of the steel post member that was used. Some of the post member had higher stresses than the yield stress of the steel member. This paper also shows an example to improve the capacity of the post members using increased cross-section members. Based on the analyses results, when investigating the safety of the transmission tower, one must consider thenew design philosophy including ultimate strength of the member and reliability of the special loading cases.

• Polymer(Korea)
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• v.26 no.5
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• pp.691-700
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• 2002

Interstitial therapy using biodegradable polymeric device loaded with anticancer agent can deliver the drug to the tumor site at high concentration, resulting in an increase of therapeutic efficacy. 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU, carmustine) is most commonly used as chemotherapeutic agent for brain tumors. The design of implantable device is regarded as an important factor lot the efficient delivery of antitumor agent to targeting site. In order to control the release profile of drug, the release pattern of BCNU with the changes of various dimension and additives was investigated. The PLGA wafers containing 3.85, 10, 20 and 30% of BCNU were prepared in various shape (diameter of 3, 5 and 10 mm, thickness of 0.5, 1 and 2 mm) by direct compression method. In vitro drug release profile of BCNU-loaded PLGA wafers could be controlled by changing the dimension of wafers such as initial drug content, weight, diameter, thickness, volume and surface area of wafers, as well as PLGA molecular weight and additives. Drug release from BCNU-loaded PLGA wafers was facilitated with an increase of BCNU-loading amount or presence of poly(N-vinylpyrrolidone)(PVP) or sodium chloride (NaCl). The effects of various geometric factors and additives on the BCNU release pattern were confirmed by the investigation of mass loss and morphology of BCNU-loaded PLGA wafers.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Applied Chemistry for Engineering
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• v.23 no.6
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• pp.546-553
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• 2012

Urushiol extracted from lacquer tree exhibits good thermal stabilities as well as antimicrobial andantioxidant properties. However, it has been known that the urushiol derivates bring out allergy. In this study, polyurushiol (PUOH) powders were successfully synthesized for the safe and convenient handling of allergic urushiol. First, the as-synthesized PUOH was confirmed by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), thermal gravimetric analyzer (TGA), antioxidant test and antimicrobial test. And then, six different LDPE/PUOH composite films were prepared via a twin screw extruder system and investigated their feasibility to use as active packaging materials. Their chemical structures, morphology, thermal optical and antimicrobial properties of the LDPE/PUOH composite films were investigated as a function of PUOH contents. FTIR and SEM results showed that LDPE/PUOH composite films have a weak interfacial interaction and poor dispersion with a high PUOH loading. The thermal properties increased up to 3 wt% as the content of PUOH increases. Compared to the pure LDPE films, LDPE/PUOH composite films are more effective in the UV absorbance and antibacterial activity against E. coli. To maximize the performance of LDPE/PUOH compositefilms as the packaging materials, further researches are required to enhance the dispersion of PUOH powders in the LDPE matrix.