• Clinical and Experimental Reproductive Medicine
• /
• v.29 no.3
• /
• pp.201-207
• /
• 2002

Objective: To observe the capability of fertilization and embryo development including blastocyst formation of the oocytes in simple media after thawing of the cryopreserved cumulus-free mouse oocytes by vitrification method. Methods: Oocytes were collected from 5 to 6 weeks old ICR female mice, and were denuded from the cumulus cells by 0.1% hyaluronidase. Recovered mature oocytes in study group were cryopreserved by vitrification method using EM grid for $5{\sim}7$ days. In brief, oocytes were exposed in dPBS containing 1.5 M EG and 5.5 M EG+1 M sucrose for 2.5 minutes and 20 seconds each, and then executed vitrification by plunging in LN2 after loading on EM grid. Thawing treated by exposure of 1, 0.5, 0.25 and 0.125 M sucrose solution for 2.5 minutes each in order and used for experiments. Spermatozoa aspirated form the epididymis of 12 weeks old ICR male mice were used for insemination after capacitation. T6 media containing 0.4% BSA were used for fertilization and development. Results: Survival and fertilization rates after thawing were 76.9% and 79.6% respectively. Fertilization rate was lower (p<0.005) than that of control group (92.9%). There was no difference in embryo developmental rates from 2-cell to morula, however, the blastocyst formation rate and mean cell numbers of blastocysts in study group (63.3%, $58.9{\pm}9.2$) were lower compared with those of control group (76.1%, $63.5{\pm}8.9$). Conclusion: Vitrification is an effective method for mouse mature oocyte cryopreservation with high survival and fertilization rate after thawing. And in simple media, fertilization rates and embryo development of frozen-thawed mouse oocytes are satisfactory.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.26 no.2
• /
• pp.139-148
• /
• 2020

The most important factor affecting the berthing energy generated when a ship berths is the berthing velocity. Thus, an accident may occur if the berthing velocity is extremely high. Several ship features influence the determination of the berthing velocity. However, previous studies have mostly focused on the size of the vessel. Therefore, the aim of this study is to analyze various features that influence berthing velocity and determine their respective importance. The data used in the analysis was based on the berthing velocity of a ship on a jetty in Korea. Using the collected data, machine learning classification algorithms were compared and analyzed, such as decision tree, random forest, logistic regression, and perceptron. As an algorithm evaluation method, indexes according to the confusion matrix were used. Consequently, perceptron demonstrated the best performance, and the feature importance was in the following order: DWT, jetty number, and state. Hence, when berthing a ship, the berthing velocity should be determined in consideration of various features, such as the size of the ship, position of the jetty, and loading condition of the cargo.

• Journal of Korean Society of Environmental Engineers
• /
• v.27 no.10
• /
• pp.1035-1042
• /
• 2005

Granular Activated Carbon(GAC) is widely used in drinking water treatment. At S and B Water Treatment Plant, GAC is used in place of granular media in conventional rapid filters(GAC Filter-Adsorber) for removal of Disinfection By-products(DBPs). The primary focus of this study is on the performance of existing filter-adsorber, and their operation. It was found that F/A process removed turbidity as effective as sand system. The ratio of Hydrophobic DOM (HPO) and hydrophilic DOM (HPI) fraction in the raw water at S and B WTP was similar. Filter Adsorber presented earlier DOC breakthrough and steady state condition which was contributed by biodegradation during operation period. The removal efficiency of DBPs were used to evaluate the filter performance. The DBPs concentration of F/A treated water was below treatment goal level (THM < $80\;{\mu}g/L$, HAA < $60{\mu}g/L$). The removal efficiency of THM decreased rapidly during operation period. However, HAA were removed steadily regardless of the influent concentration of HAA. These results indicate that the removal of THM depend upon the adsorption mechanism while the removal of HAA depend upon biodegradation as well as adsorption. The decrease of adsorption capacity and characteristic value of GAC may be attributed to the effect of high organic loading, residual free chlorine, coagulants, manganese oxidants and frequently backwashing. This study has confirmed that Filter adsorber process can be considered as effective alternatives for the removal of DBPs, especially HAA.

• Journal of Korean Society of Environmental Engineers
• /
• v.31 no.5
• /
• pp.341-345
• /
• 2009

Continuous dry anaerobic digestion of organic solid wastes (30% TS, Total Solids) comprised of food waste and paper was performed under mesophilic condition. During the operation, hydraulic retention time (HRT) was decreased as follows: 150 d, 100 d, 60 d, and 40 d, which corresponded to the solid loading rate of 2.0, 3.0, 5.0, and 7.5 kg TS/$m^3$/d, respectively. Volumetric biogas production rate ($m^3$/$m^3$/d) increased as HRT decreased, and the highest biogas production rate of 3.49${\pm}$0.31 $m^3$/$m^3$/d was achieved at 40 d of HRT. At this HRT, high volatile solids (VS) reduction of 76% was maintained, and methane production yield of 0.25 $m^3$/kg $TS_{added}$ was achieved, indicating 67.4% conversion of organic solid waste to bioenergy. The highest biogas production yield of 0.52 $m^3$/kg $TS_{added}$ was achieved at 100 d of HRT, but it did not change much with respect to HRT. For the ease feed pumping, some amount of digester sludge was recycled and mixed with fresh feed to decrease the solid content. Recirculation volume of 5Q was found to be the optimal in this experimental condition. Specific methanogenic activity (SMA) of microorganisms at mesophilic-dry condition was 2.66, 1.94, and 1.20 mL $CH_4$/g VS/d using acetate, butyrate, and propionate as a substrate, respectively.

• Journal of Korean Society of Environmental Engineers
• /
• v.36 no.4
• /
• pp.258-264
• /
• 2014

This study is to investigate water quality changes of nitrogen (N) and phosphorous (P) at Gangjung-Goryeong Weir and Dalseong weir, in 2012-2013. Concentration of COD, TOC, T-N, T-P showed 3.5~6.8 mg/L, 3.4~6.5 mg/L, 1.8~4.0 mg/L, 0.08~0.12 mg/L respectively, in the Nakdong river main stream. Water quality of Dalseong weir showed lower level of contamination than that of Gangjung-Goryeong weir. Because of input loading of nutrients and organic matters from tributaries. At the analysis of sediments at each weirs, sedimentation fluxes of the hypolimnion represented 1.6~2.4 times higher than epilimnion fluxes. Sinking rate (%/d) of SS, N and P of the hypolimion showed in the range of 1.68 %/d~2.42 %/d. It is implied that the suspended matters seem to be floating in the water body. In the result of nutrients release experiment, release flux of nutrients in July showed 3~4 times higher than April. The reason was considered that nutrients is easily released in the anaerobic condition and at high water temperature.

• Elastomers and Composites
• /
• v.49 no.1
• /
• pp.43-52
• /
• 2014

The effect of nanoclay (Cloisite 20A) on the self-adhesion behavior of uncured brominated-isobutylene-isoprene rubber (BIIR) has been studied. The dispersion state of nanoclay into the rubber matrix was examined by SEM, TEM and XRD analysis. The thermal degradation behavior of the filled and unfilled samples was examined by TGA and improvement in the thermal stability of the nanocomposites occurred based on the weight loss (%) measurements. Also, addition of nanoclay enhanced the cohesive strength of the material by reinforcement action thereby reducing the degree of molecular diffusion across the interface of butyl rubber. However, the average depth of penetration of the inter-diffused chains was still adequate to form entanglement on either side of the interface, and thus offered greater resistance to peeling, resulting in high tack strength measurements. The improvement in tack strength was only achieved at critical nanoclay loading above 8 phr. Contact angle measurement was also made to examine the surface characteristics. There was no significant interfacial property change by employing the nanoclay.

• Proceedings of the KSRS Conference
• /
• 2006.03a
• /
• pp.341-343
• /
• 2006

The current spectral shape matching method (SSMM), developed by Ahn and Shanmugam (2004), relies on the assumption that the path radiance resulting from scattered photons due to air molecules and aerosols and possibly direct-reflected light from the air-sea interface is spatially homogeneous over the sub-scene of interest, enabling the retrieval of water-leaving radiances ($L_w$) from the satellite ocean color image data. This assumption remains valid for the clear atmospheric conditions, but when the distribution of aerosol loadings varies dramatically the above postulation of spatial homogeneity will be violated. In this study, we present the second version of SSMM which will take into account the horizontal variations of aerosol loading in the correction of atmospheric effects in SeaWiFS ocean color image data. The new version includes models for the correction of the effects of aerosols and Raleigh particles and a method fur computation of diffuse transmittance ($t_{os}$) as similar to SeaWiFS. We tested this method over the different optical environments and compared its effectiveness with the results of standard atmospheric correction (SAC) algorithm (Gordon and Wang, 1994) and those from in-situ observations. Findings revealed that the SAC algorithm appeared to distort the spectral shape of water-leaving radiance spectra in suspended sediments (SS) and algal bloom dominated-areas and frequently yielded underestimated or often negative values in the lower green and blue part of the electromagnetic spectrum. Retrieval of water-leaving radiances in coastal waters with very high sediments, for instance = > 8g $m^{-3}$, was not possible with the SAC algorithm. As the current SAC algorithm does not include models for the Asian aerosols, the water-leaving radiances over the aerosol-dominated areas could not be retrieved from the image and large errors often resulted from an inappropriate extrapolation of the estimated aerosol radiance from two IR bands to visible spectrum. In contrast to the above results, the new SSMM enabled accurate retrieval of water-leaving radiances in a various range of turbid waters with SS concentrations from 1 to 100 g $m^{-3}$ that closely matched with those from the in-situ observations. Regardless of the spectral band, the RMS error deviation was minimum of 0.003 and maximum of 0.46, in contrast with those of 0.26 and 0.81, respectively, for SAC algorithm. The new SSMM also remove all aerosol effects excluding areas for which the signal-to-noise ratio is much lower than the water signal.

• Journal of Korean Academy of Nursing
• /
• v.28 no.3
• /
• pp.751-759
• /
• 1998

The study of the validity test on the self-monitoring scale for nurses In this study, both the literary survey as well as empirical research has been executed to test the validity of the scales that measure the construct of the self-monitoring scale. The self-monitoring scale could not be classified into five factors as Snyder suggested. Many other scholars (Briggs, Cheek and Buss, 1980) suggested 3 different classifications which was accepted by Snyder and Gangestad (1986). John, Cheek and Klohnen(1996) claimed a two-factor classification. As has been discussed, factor analysis is used to prove convergent validity within the factor and discriminant validity between the factors. However, depending on the researchers, many variations in classification of the factors were found and a lack of content and discriminant validity were found in the previous research findings. It is also important to note that Snyder's self-monitoring scale did not factor-load at over. 30 for all 25 items, regardless of how many factors could be classified. According to findings of this study, the self-monitoring scale neither classified as five, three or two factors nor factor loaded as hypothesized. It is also clear that Snyder's self-monitoring scale lacks convergent validity as the sub-factors of the scale failed to prove its uni-dimensionality. The A self-monit oring scale not only fail to overcome the problems of Snyder's self-monitori ng scale but even lost the attractiveness of the self-monitoring scale. In this study it was also found that the A self-monitoring scale was not classified in either in a two or three-factor classification as hypothesized. It is, of course, not desirable to use any scale that lacks convergent and discriminant validity even though it has been widely used and has held a great deal of influence on the field of social psychology. To overcome the shortcomings of Snyder's self-monitoring scale, Lennox and Wolfe(1984) suggested 13 items. This study was dedicated to test the validity and reliability of the scale, in which we found that the data presented in validity as the two factors were class ified and loaded as expected. Reliability was also proven by checking Cronbach's α for each factor and for the total items. In addition, a confirmatory factor analysis was executed for the 13 items using LISREL 8.12 program to confirm convergent validity in a two-factor classification. The model was fitting and sound : however, the self-monitoring scale was unfitted and not validated. Thus, it is recommended to use not the original nor the abbreviated self-monitoring scale but the 13 items in future studies. It should also be noted that items 7 and 13 should be removed to obtain better uni-dimensionality for the 13 items. These items loaded at over. 30, too high for the two factors in the test results of Factor analysis. In addition, it is necessary to double-check the cause of two-hold loading at over .30 for the two factors. It could be a problem caused by data or by the scale itself. Therefore, additional studies should follow to better clarify this matter.

• Journal of Korea Port Economic Association
• /
• v.28 no.4
• /
• pp.275-298
• /
• 2012

Terminal operators have provided special services such as loading, discharging, stowage of cargo to the owner, and carriers of the sea, which contribute the domestic and international logistics. For smooth flow of logistics, container terminal should reserve spaces for inbound and outbound logistics. However, if it is unable to provide the spaces, which can be caused by labour strikes or terminal lockouts and so on, national logistic system and financial management of terminal operators can be seriously affected. In order to minimize these kinds of problems, terminal operators impose high rate of charges ("overstorage charge") to the accumulated cargoes and/or containers, which are stipulated in terminal service agreement. Nevertheless, if there is no terminal service agreement with an owner of cargo and/or container, any kind of charge can cause legal problems (conflict ??) between the cargo and terminal operator. In this regard, I would like to study on the definition of overstorage charge and the legal issue of it based on the Busan district court's judgment. In particular, I will propose a special right of commercial lien and public auction for terminal operators to settle accumulated cargoes in container terminal.

• Journal of Korean Society of Steel Construction
• /
• v.20 no.2
• /
• pp.237-246
• /
• 2008

The cross-sections of continuous multi-span beams sometimes suddenly increase, or become stepped, at the interior supports of continuous beams to resist high negative moments. The three-dimensional finite-element program ABAQUS (2006) was used to analytically investigate the inelastic lateral-torsional buckling behavior of stepped beams subjected to pure bending moment and resulted in the development of design equations. The flanges of the smaller cross-section were fixed at 30.48 by 2.54 cm, whereas the width and/or thickness of the flanges of the larger cross-section varied. The web thickness and height of beam was kept at 1.65 cm and 88.9 cm, respectively. The ratios of the flange thickness, flange width, and stepped length of beams are considered analytical parameters. Two groups of 27 cases and 35 cases, respectively, were analyzed for double and single stepped beams. The combined effects of residual stresses and geometrical imperfection on inelastic lateral-torsional buckling of beams are considered. First, the distributions of residual stress of the cross-section is same as shown in Pi, etc (1995), and the initial geometric imperfection of the beam is set by central displacement equal to 0.1% of the unbraced length of beam. The new proposed equations definitely improve current design methods for the inelastic LTB problem and increase efficiency in building and bridge design. The proposed solutions can be easily used to develop new design equation for inelastic LTB resistance of stepped beams subjected to general loading condition such as a concentrated load, a series of concentrated loads or uniformly distributed load.