• KSBB Journal
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• v.5 no.3
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• pp.269-277
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• 1990

Methylobacillus sp. SK1, an obligate methylotroph, was cultivated in a fed-batch culture using DO as a methanol feeding indicator with a micro computer-aided control system. While 2.07g/l of cell density was obtained after 13 hr in the batch culture (initial methanol concentration: 1.0%(v/v)),45.3g/l of cell density was obtained after 17 hr by feeding methanol and metal ions in the fed-batch culture with oxygen supply. The high-density biomass was obtained in short cultuivation time by fed-batch culture with feedback control, and consequently the biomass productivity was significantly increased. It was mainly due to extension of logarithmic growth period by methanol feeding without methanol inhibition and intensive aeration without DO limitation with microcomputer-aided control system.

• Journal of Plant Biotechnology
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• v.5 no.1
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• pp.1-6
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• 2003

The adventitious root of Panax ginseng C.A. Meyer is regarded as an efficient alternative to cell culture or hairy root culture for biomass production due to its fast growth and stable metabolite production. To determine optimal culture conditions for the bioreactor culture of ginseng roots, experiments have been conducted on physical and chemical factors such as bioreactor type, dissolved oxygen, gas supply, aeration, medium type, macro- and micro-elements, medium supplement during culture period, sucrose concentration, osmotic agents, medium pH and light. Elicitation is a key step to increase ginsenoside accumulation in the adventitious roots but biomass growth is severely inhibited by elicitor treatment. To obtain high ginsenoside content with avoiding biomass decrease, we applied two-stage bioreactor culture system. Ginseng adventitious roots were cultured for 40 days to maximize biomass increase followed by elicitation for 7 days to enhance ginsenoside accumulation. We also experimented on types and concentrations of jasmonate to determine optimal elicitation methods. In this paper, we discussed several factors affecting the root propagation and ginsenoside accumulation. Based on the results obtained from previous experiments we have established large-scale bioreactor system (1 ton-10 ton) for the efficient production of ginseng adventitious roots and bioactive compounds including ginsenoside. Still, experiments are on going in our laboratory to determine other bioactive compounds having effects on diet, high blood pressure, DPPH elimination and increasing memories.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.12 no.1
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• pp.1-10
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• 2000

A three-dimensional hydrodynamic-ecosystem model was developed and applied to Chinhae Bay which is located in the southeastern sea of Korea. The model includes a three-dimensional hydrodynamic model and an eutrophication model, and the model operates on the same grid system. The agreement between predicted and measured results is reasonably encouraging. The concentrations of the calculated COD, DIN and DIP are appeared to be very high due to the phytoplankton production and the wastewater input in the northern part of Chinhae Bay. Anoxic and hypoxic water masses in the bottom layer occur in the northern part of the bay due to the excess loading of wastewater and strong stratification, and in the western inner part of the bay due to high oxygen consumption in densely populated aquaculturing facilities. DO concentration contours show parallel to the bay entrance line, which means the importance of supplying DO by physical process from the mouth of the bay. Although both the hydrodynamic and biochemical processes play important role to form the hypoxic waters in the bottom of the inner bay, it is suggested that the hydrodynamic conditions such as the vertical and the horizontal eddy diffusivity are primarily important factors.

• Korean Journal of Materials Research
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• v.19 no.8
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• pp.427-431
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• 2009

ZnO wire-like thin films were synthesized through thermal oxidation of sputtered Zn metal films in dry air. Their nanostructure was confirmed by SEM, revealing a wire-like structure with a width of less than 100 nm and a length of several microns. The gas sensors using ZnO wire-like films were found to exhibit excellent $H_2$ gas sensing properties. In particular, the observed high sensitivity and fast response to $H_2$ gas at a comparatively low temperature of $200^{\circ}C$ would lead to a reduction in the optimal operating temperature of ZnO-based $H_2$ gas sensors. These features, together with the simple synthesis process, demonstrate that ZnO wire-like films are promising for fabrication of low-cost and high-performance $H_2$ gas sensors operable at low temperatures. The relationship between the sensor sensitivity and $H_2$ gas concentration suggests that the adsorbed oxygen species at the surface is $O^-$.

• Journal of Korean Society on Water Environment
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• v.30 no.4
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• pp.394-402
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• 2014

High-voltage dielectric discharges are an emerging technique in environmental pollutant degradation, which are characterized by the production of hydroxyl radicals as the primary degradation species. The initiation and propagation of the electrical discharges depends on several physical, chemical, and electrical parameters such as 1st and 2nd voltage of power, gas supply, conductivity and pH. These parameters also influence the physical and chemical characteristics of the discharges, including the production of reactive species such as OH, $H_2O_2$ and $O_3$. The experimental results showed that the optimum 1st voltage and oxygen flow rate for RNO (N-Dimethyl-4-nitrosoaniline, indicator of the generation of OH radical) degradation were 160 V (2nd voltage of is 15 kV) and 4 L/min, respectively. As the 2nd voltage (4 kV to 15 kV) was increase, RNO degradation was increased and, generated $H_2O_2$ and $O_3$ concentration were increased. The conductivity of the solution was not influencing the RNO degradation, $H_2O_2$ and $O_3$ generation. The pH effect on RNO degradation was not high. However, the lower pH and the conductivity, the higher $H_2O_2$ and $O_3$ generation were observed.

• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.149-153
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• 2004

L-Threonine fermentation process was constructed on batch and fed-batch culture by using Escherichia coli MT201. The production type of L-threonine was observed as growth-associated production in batch culture. In fed-batch culture studying optimal concentration of yeast extract in feeding media, when 600 g/l of glucose and 60 g/l of yeast extract were added in feeding media, 87 g/$\ell$ of L-threonine was produced. To improve cell growth and L-threonine production, the culture of high cell density was performed in fed-batch culture with oxygen enriched air and feeding media containing L-methionine and phosphate. Under the conditions, we could achieve the highest L-threonine production of98 g/$\ell$ at 60 h. The highest productivity of L-threonine was about 3.85 g/$\ell$/h.

• Development and Reproduction
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• v.23 no.1
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• pp.21-34
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• 2019

The effects of the anaesthetic agents, clove oil and mixture of clove oil with lidocaine-HCl were evaluated on river puffer, Takifugu obscurus and tiger puffer, T. rubripes. Anaesthesia times of clove oil were affected by water temperature ($20^{\circ}C$, $24^{\circ}C$, and $28^{\circ}C$) and salinity (10, 20, and 30 ppt). Anaesthesia times of mixed samples were significantly similar with regard to exposure and recovery times, and all samples satisfied anaesthesia criteria (exposure time within 3 min and recovery time within 5 min) under the various temperatures and salinities, and the lowest to highest concentration of anaesthetics (p<0.05). Both species river puffer and tiger puffer had short exposure time with a high anaesthesia dose, high temperature ($28^{\circ}C$) and intermediate salinity (20 ppt), and were highly affected by temperature and salinity (p<0.05). The mixed anaesthetics had rapid exposure times and long recovery times in contrast to the effects of clove oil. Cortisol concentrations under the conditions of various clove oil dosages, salinity, and temperature for both species increased until 12 hrs after recovery from anaesthesia (p<0.05). After 12 hrs, cortisol concentrations decreased until after 48 hrs (p<0.05). During the simulated transportation of both species, control and sedated clove oil groups (5 ppm) were measured for water parameters, dissolved oxygen (DO), $CO_2$, respiratory frequency, $NH_4{^+}$, and pH for 6 hrs in 1 hr intervals. Water parameters of sedated groups and controls were significantly different after 2 hrs (p<0.05).

• Journal of Korean Society on Water Environment
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• v.27 no.4
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• pp.533-541
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• 2011

Algal blooms have caused problems for drinking water as well as eutrophication. However it is difficult to control algal blooms by current warning manual in rainy season because the algal blooms happen in a few days. The water quality data, which have high correlations with Chlorophyll-a on Daecheongho station, were analyzed and chosen as input data of Artificial Neural Networks (ANN) for training pattern changes. ANN was applied to early forecasting of algal blooms, and ANN was assessed by forecasting errors. Water temperature, pH and Dissolved oxygen were important factors in the cross correlation analysis. Some water quality items like Total phosphorus and Total nitrogen showed similar pattern to the Chlorophyll-a changes with time lag. ANN model (No. 3), which was calibrated by water temperature, pH and DO data, showed lowest error. The combination of 1 day, 3 days, 7 days forecasting makes outputs more stable. When automatic monitoring data were used for algal bloom forecasting in Daecheong reservoir, ANN model must be trained by just input data which have high correlation with Chlorophyll-a concentration. Modular type model, which is combined with the output of each model, can be effectively used for stable forecasting.

• Journal of Korean Society on Water Environment
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• v.27 no.6
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• pp.855-861
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• 2011

Short-term effects of current velocity and turbid water on the benthic diatom community and water quality were examined in artificial channel ($20{\times}200{\times}10cm$) with two different experiments. The first and second experiments were consisted of different current velocities such as 1 L/min., and 1, 3, and 6 L/min., respectively. The concentration of turbid water is prepared with loess and fixed at 10 and 20 times of the turbidity of control inflow (10 NTU, LTW), respectively. At experiment 1 (EXP-1), introduction of turbid water increased dissolved oxygen, electric conductivity, pH and turbidity, but there were no differences between low- (100 NTU, MTW) and high-turbid water (200 NTU, HTW). However, experiment 2 (EXP-2) did not change any environmental parameters except dissolved total and inorganic nitrogen like EXP-1. MTW in EXP-1 strongly stimulated the growth of benthic diatom, while both MTW (150 NTU) and HTW (300 NTU) in EXP-2 did not increase or decrease the diatom abundance. Over the study, the dominant species was four, Aulacoseira ambigua, Cyclotella stelligera, Aulacoseira granulata and Achnanthes minutissima. In EXP-1, two highest species in abundance, A. ambigua and A. granulata were highly grown in MTW, while Achnanthes minutissima high in HTW adversely. These results indicate that the introduction of turbid water can play an important role in the shift of water quality and benthic diatom community in stream ecosystem, especially inflow of soil water in low current velocity.

• Food Science and Industry
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• v.50 no.2
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• pp.12-26
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• 2017

Processing steps such as washing and cutting, involved in preparing fresh-cut produce causes tissue damage, leading to rapid quality deterioration. Major defects of fresh-cut produce are discoloration, softening, off-odor development, and microbial growth. Packaging of fresh-cut produce has been changed to reduce these quality problems. Flexible packaging film is widely used to pack fresh-cut produce. Vacuum packaging was the popular packaging method in the beginning of fresh-cut industry in Korea. Vacuum packaging creates high $CO_2$ and low $O_2$ levels to control browning of fresh-cut produce. However, these conditions induce some visual defects and off-odor development. Discoloration problem was also found when fresh-cut produce was packaged with conventional packaging film or plastic tray. Modified atmosphere (MA) packaging is effective for prolonging shelf-life of fresh-cut produce by decreasing $O_2$ and increasing $CO_2$ concentration in the package. Retail MA packaging using different oxygen transmission rate (OTR) film and micro-perforated film has started to be applied to fresh-cut produce in Korea. Proper MA package design that provides optimum range of $O_2$ and $CO_2$ partial pressures is one of the major challenges in the industry. An initial package flushing with $N_2$ or an low $O_2$/high $CO_2$ atmosphere is also used to more rapidly establish steady-state MA condition. Film OTR and $O_2$ flushing affects the fermentative volatile production, off-odor development, electrolyte leakage, discoloration, $CO_2$ injury, microbial population of fresh-cut produce. There is also a demand for convenient packaging to attract consumers. Rigid fresh-cut produce container for retail market has increased since the packaging provides excellent protection from physical damage during transport. Rigid tray used as actual serving vessel for the consumer is increasing in Korea. The tray with flexible lid to wrap or seal fresh-cut produce is more and more gaining popularity. Further practical technology to control quality change and microbial growth for each fresh-cut product has been studied since various fresh-cut items were required. The fresh-cut industry also focuses on searching for more convenient and environmentally friendly packaging.