• Title/Summary/Keyword: High sensitive

Search Result 3,130, Processing Time 0.033 seconds

CHEMOSENSITIVITY OF CANCER CELLS TO ANTICANCER DRUGS USING DYE EXCLUSION ASSAY, [3H] THYMIDINE INCORPORATION, AND CLONOGENIC ASSAY (두경부악성종양세포주의 항암제감수성 시험에 관한 실험적 연구)

  • Jin, Woo-Jeong
    • Maxillofacial Plastic and Reconstructive Surgery
    • /
    • v.15 no.1
    • /
    • pp.35-48
    • /
    • 1993
  • The in vitro predictive tests in cancer chemotherapy of cancer cell lines to anticancer drugs were determined using novel dye exclusion assay [NDEA], [3H] thymidine incorporation, and clonogenic assay [CA>. Antitumor effect of Bleomycin, Cis-platin, Vinblastine, Methotrexate to HEp-2, B16 cell lines using rapid assays was compared with [CA> in this study. In dye exclusion assay of B l6 cell line, cancer cells were sensitive to Bleomycin at all concentrations, to Vinblastine at the level of peak plasma concentration [PPC], ${\times}1/10$ [PPC](P<0.05). And Bleomycin revealed relatively good cytotoxicity than that of CDDP and vinblastine at ${\times}10$[PPC], (P<0.05). HEp-2 cells were resistive to methotrexate at the level of ${\times}100$[PPC] (P<0.05) In [3H] thymidine incorporation assay, B 16 cells were sensitive to Bleomycin, CDDP, Vinblastine at the level of [PPC], ${\times}10$ [PPC](P<0.01). Dose-dependent drugs of bleomycin, CDDP were more sensitive than Vinblastine at high concentration (P<0.05). In clonogenic assay, HEp-2 cell line was sensitive to three drugs of all concentrations except ${\times}10$ [PPC] of CDDP. B 16 cell line was sensitive to all drugs(P<0,01). In comparison of chemosensitivity tests among three assays, the results were correlated(${\gamma}=0.99$, P<0.05).

  • PDF

Distinction between Cold-sensitive and -tolerant Jute by DNA Polymorphisms

  • Hossain, Mohammad Belayat;Awal, Aleya;Rahman, Mohammad Aminur;Haque, Samiul;Khan, Haseena
    • BMB Reports
    • /
    • v.36 no.5
    • /
    • pp.427-432
    • /
    • 2003
  • Jute is the principal coarse fiber for commercial production and use in Bangladesh. Therefore, the development of a high-yielding and environmental-stress tolerant jute variety would be beneficial for the agro economy of Bangladesh. Two molecular fingerprinting techniques, random-amplified polymorphic DNA (RAPD) and amplified-fragment length polymorphism (AFLP) were applied on six jute samples. Two of them were cold-sensitive varieties and the remaining four were cold-tolerant accessions. RAPD and AFLP fingerprints were employed to generate polymorphism between the cold-sensitive varieties and cold-tolerant accessions because of their simplicity, and also because there is no available sequence information on jute. RAPD data were obtained by using 30 arbitrary oligonucleotide primers. Five primers were found to give polymorphism between the varieties that were tested. AFLP fingerprints were generated using 25 combinations of selective-amplification primers. Eight primer combinations gave the best results with 93 polymorphic fragments, and they were able to discriminate the two cold-sensitive and four cold-tolerant jute populations. A cluster analysis, based on the RAPD and AFLP fingerprint data, showed the population-specific grouping of individuals. This information could be useful later in marker-aided selection between the cold-sensitive varieties and cold-tolerant jute accessions.

Light intensity controlled wrinkling patterns in photo-thermal sensitive hydrogels

  • Toh, William;Ding, Zhiwei;Ng, Teng Yong;Liu, Zishun
    • Coupled systems mechanics
    • /
    • v.5 no.4
    • /
    • pp.315-327
    • /
    • 2016
  • Undergoing large volumetric changes upon incremental environmental stimulation, hydrogels are interesting materials which hold immense potentials for utilization in a wide array of applications in diverse industries. Owing to the large magnitudes of deformation it undergoes, swelling induced instability is a commonly observed sight in all types of gels. In this work, we investigate the instability of photo-thermal sensitive hydrogels, produced by impregnating light absorbing nano-particles into the polymer network of a temperature sensitive hydrogel, such as PNIPAM. Earlier works have shown that by using lights of different intensities, these hydrogels follow different swelling trends. We investigate the possibility of utilizing this fact for remote switching applications. The analysis is built on a thermodynamic framework of inhomogeneous large deformation of hydrogels and implemented via commercial finite element software, ABAQUS. Various examples of swelling induced instabilities, and its corresponding dependence on light intensity, will be investigated. We show that the instabilities that arise have their morphologies dependent on the light intensity.

Molecular Detection of Verotoxigenic Escherichia coli (VTEC) from Animal Feces for Screening VTEC-shedders

  • Kobayashi, Y.;Sato, M.;Taguchi, H.;Koike, S.;Nakatsuji, H.;Tanaka, K.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.17 no.3
    • /
    • pp.423-427
    • /
    • 2004
  • Seventy-six animals including cattle, sheep, horses, 6 species of zoo animals were employed for collection of fresh feces in order to detect verotoxigenic Esherichia coli (VTEC) by safe, quick and sensitive PCR-based molecular methods. Bacterial cell disruption with bead-beating followed by bacterial DNA purification with hydroxyapatide chromatography and gel filtration allowed DNA preparation from animal feces with high recovery and purity. A mountain goat was firstly shown by PCR and sequencing to shed verotoxin 2 gene (vt2) that was used to generate vt2 probe and second primer set for nested PCR to attempt more sensitive detection. Most sensitive nested PCR revealed that 45% of tested cattle and 47% of tested zoo animals were VTEC-positive, while least sensitive normal PCR detected VTEC from none of these animals except a mountain goat. Moderately sensitive detection by PCR in combination with hybridization suggested that the VTEC density varied between the VTEC-positive cattle.

Isolation and Identification of Antimicrobial Agent Producing Microorganisms and Sensitive Strain from Soil (토양으로부터 항균물질 생성균 및 감수성 균주의 분리 및 동정)

  • 장해춘;김수인;김인철
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.28 no.3
    • /
    • pp.526-533
    • /
    • 1999
  • Two species of antimicrobial agent producing bacteria and one sensitive strain were isolated from soil. Those were identified as B. subtilis, B. licheniformis, and Curtobacterium sp. by morphological, biochemical, physiological and chemotaxonomic characteristics. These were designated as B. subtilis cx1, B. licheniformis cy2 and Curtobacterium sp. cf3, respectively. Antimicrobial agent produced by B. subtilis cx1 showed high antibacterial activity against gram positive bacteria including of B. subtilis, Curtobacterium sp., L. mesenteroids, Staphy. aureus, S. faecalis and even gram negative bacteria, P. aeruginosa. Antimicrobial agent from B. licheniformis cy2 showed slightly lower antimi crobial activity than that from B. subtilis cx1. These two strains showed maximum production of antimicrobial agents at 30oC for 9~21hr cultivation. Curtobacterium sp. cf3 showed more sensitive activity than a sensitive strain of B. subtilis ATCC 6633 which was same genus or species with the B. subtilis cx1 and B. subtilis cy2, when the antimicrobial agent producing strains, B. subtilis cx1 and B. subtilis cy2, were directly applied onto these sensitive lawns.

  • PDF

A Task Scheduling Strategy in Cloud Computing with Service Differentiation

  • Xue, Yuanzheng;Jin, Shunfu;Wang, Xiushuang
    • KSII Transactions on Internet and Information Systems (TIIS)
    • /
    • v.12 no.11
    • /
    • pp.5269-5286
    • /
    • 2018
  • Task scheduling is one of the key issues in improving system performance and optimizing resource management in cloud computing environment. In order to provide appropriate services for heterogeneous users, we propose a novel task scheduling strategy with service differentiation, in which the delay sensitive tasks are assigned to the rapid cloud with high-speed processing, whereas the fault sensitive tasks are assigned to the reliable cloud with service restoration. Considering that a user can receive service from either local SaaS (Software as a Service) servers or public IaaS (Infrastructure as a Service) cloud, we establish a hybrid queueing network based system model. With the assumption of Poisson arriving process, we analyze the system model in steady state. Moreover, we derive the performance measures in terms of average response time of the delay sensitive tasks and utilization of VMs (Virtual Machines) in reliable cloud. We provide experimental results to validate the proposed strategy and the system model. Furthermore, we investigate the Nash equilibrium behavior and the social optimization behavior of the delay sensitive tasks. Finally, we carry out an improved intelligent searching algorithm to obtain the optimal arrival rate of total tasks and present a pricing policy for the delay sensitive tasks.

Clinical Usefulness of 99mTc-DMSA Renal SPECT Using High Sensitivity-All Purpose Collimator for Pediatric Patients (고감도 범용성 콜리메이터를 이용한 소아 환자 99mTc-DMSA 신장 SPECT의 유용성)

  • Kim, Jin-Eui;Kim, Jung-Soo;Han, Jae-Bok;Choi, Nam-Gil
    • The Journal of the Korea Contents Association
    • /
    • v.16 no.3
    • /
    • pp.219-231
    • /
    • 2016
  • $^{99m}Tc$-DMSA planar scan that can analyze the functions of kidney quantitatively provides less information on a lesion than tomography scanning. Therefore, this study applied a high sensitivity all-purpose collimator that is sensitive to photonic signals to $^{99m}Tc$-DMSA and carried out a clinical scan with single photon emission computed tomography (SPECT). And diagnostic accuracy and time requirement of were analyzed to know the clinical usefulness of the applied scanning method. 10 subjects were intravenously injected with radiopharmaceutical product (1.0-1.2 MBq/kg) and scanned by a gamma camera with planar scanner (high resolution (HR)-mode, $256{\times}256$, 50 kcts/view, 4 image) and SPECT (HR / high sensitive (HS)-mode, $128{\times}128$, step and shoot, $180^{\circ}$, variable sec/angle, total 64 frame, OSEM reconstruction), respectively. The collected data was compared with an analysis program. The results showed that HS-mode SPECT detected total counts 1.8-5.6 times more than planar scan. Relative renal function evaluated based on the counts was not significantly different by two scanning methods (p=0.96) and it turned out that test time was shortened by 39% when HS-mode SPECT was used. Therefore, SPECT using HR, HS-mode collimator could analyze renal function more quantitatively than using planar scan and the former could diagnose the location information of a lesion more accurately than the latter as well as shortened test time requirement, which demonstrated the clinical usefulness of $^{99m}Tc$-DMSA renal SPECT using high sensitivity all purpose collimator.

Analysis of Insulation Characteristics for High-voltage Motor Stator Winding using Insulation Diagnostic Test (절연진단법을 이용한 고전압전동기 고정자권선의 절연특성분석)

  • Oh, Bong-Keun;Kang, Hyun-Goo;Kim, Hyun-Il;Lim, Kee-Joe
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2009.06a
    • /
    • pp.404-405
    • /
    • 2009
  • Insulation diagnostic tests for high voltage motor stator winding were conducted to analyze the insulation characteristics. Test motors were manufactured same factory and year(1996). Insulation characteristics of moisture winding are different from those of deteriorative winding. Insulation resistance and disscipation factor test results are sensitive to moisture winding. AC current, disscipation factor tip-up and PD test results are sensitive to deteriorative winding. Also, Capacitance value for stator winding insulation material has characteristic of increasing in moisture winding.

  • PDF

Series Voltage Compensation Systems for Voltage Sag by Using an Environmentally Friendly Ultra-capacitor (친환경 Ultra-capacitor에 의한 순시전압강하의 직렬전압보상 시스템)

  • Shon, Jin-Geun;Jeon, Hee-Jong
    • The Transactions of The Korean Institute of Electrical Engineers
    • /
    • v.58 no.4
    • /
    • pp.763-769
    • /
    • 2009
  • A series voltage compensation(SVC) system is a power-electronics controller that can protect sensitive loads from disturbance in the supply system. Especially, voltage sags are considered the dominant disturbances affecting the power quality. This paper dealt with a system of off-line type voltage sag compensation by using a bi-directional DC/DC converter of environmentally friendly ultra-capacitor. This capacitor is attached to the DC link of SVC through the high-efficiency DC/DC converter in order to compensate the DC link voltage drop during short-term power interruption as voltage sags. Therefore, in this paper, a DC/DC converter to control high-efficiency energy of ultra-capacitor and voltage sag detection algorithm of off-line type SVC systems are newly introduced. According to the results of experimental of prototype system, it is verified that the proposed system has effectiveness of voltage sag compensation using an ultra-capacitor.

A Study on the Mismatch of Time and Frequency Domain for Vibration Criteria of Sensitive Equipment (고정밀 장비의 진동허용규제치에 대한 시간 및 주파수 영역에서 나타나는 불일치 문제에 관한 연구)

  • 이홍기;김강부;백재호
    • Journal of the Semiconductor & Display Technology
    • /
    • v.1 no.1
    • /
    • pp.1-7
    • /
    • 2002
  • Modem technology depends on the reliability of extremely high precision equipments. In the production of semiconductor wafer, optical and electron microscopes, ion-beam, laser device must maintain their alignments within a sub-micrometer. This equipment requires a vibration free environment to provide its proper function. Therefore, this high technology equipments require very strict environmental vibration criteria because it is used as basic data for the design of building structure and structural dynamics of equipment. In this paper, the new approach is proposed to investigate the mismatch problem of time and frequency domain for vibration criteria of sensitive equipment. The proposed approach is based on a vibration measurement data and a relative transfer function which can be obtained by experiment or analysis.

  • PDF