Lee, Minji;Shin, Juyong;Kim, Jin Ho;Lim, Young Kyun;Cho, Hoon;Baek, Seung Ho
Korean Journal of Environmental Biology
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v.36
no.3
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pp.359-369
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2018
Harmful algal blooms (HABs) are a serious problem for public health and fisheries industries, thus there exists a need to investigate the possible ways for effective control of HABs. In the present study, we investigated the algicidal effects of a newly developed GreenTD against the HABs (Chattonella marina, Heterosigma akashiwo, Cochlodinium polykriokides, and Heterocapsa circularisquama) and non-HABs (Chaetoceros simplex, Skeletonema sp. and Tetraselmis sp.), which is focused on the different population density and concentration gradients of algicidal substances. The time series viability of target alga was assessed based on the activity of Chl. a photosynthetic efficiency in terms of $F_v/F_m$, and in vivo fluorescence (FSU). Effective control of Raphidophyta, C. marina and H. akashiwo was achieved at a GreenTD concentration of $0.5{\mu}gL^{-1}$ and $0.2{\mu}gL^{-1}$, respectively, and regrowth of both the species was not observed even after 14 days. The inhibitory ratio of the dinoflagellate, C. polykriokides was more than 80% at $0.2{\mu}gL^{-1}$ of GreenTD. H. circularisquama was constantly affected in the presence of $0.2{\mu}gL^{-1}$ of GreenTD in the high- and low-population density experimental groups. On the other hand, diatoms, C. simplex, and Skeletonema sp. were not significantly affected even in the presence of $0.2{\mu}gL^{-1}$ of GreenTD and exhibited re-growth activity with the passage of incubation time. In particular, green alga Tetraselmis sp. remained unaffected even in the presence of the highest concentration of GreenTD ($1.0{\mu}gL^{-1}$), implying that non-HABs were not greatly influenced by the algicidal substances. As a result, the algicidal activity of GreenTD on the harmful and nonharmful algae was as follows: raphidophyte>dinoflagellates>diatoms>green alga. Consequently, our results indicate that inoculation of GreenTD substances into natural blooms at a threshold concentration ($0.2{\mu}gL^{-1}$) can maximize the algicidal activity against HABs species. If we consider the dilution and diffusion rate in the field application, it is hypothesized that GreenTD will demonstrate economic efficiency, thus leading to effective control against the target HABs in the closed bay.
We investigated the characteristics of fish communities and habitat status of endangered species Rhodeus pseudosericeus in Heuk Stream, a tributary of the Han River, from April to October 2018. During this period, we collected 47 species of 14 families from 10 survey stations using kick nets, cast nets, and long bag set nets. The dominant and subdominant species were Zacco platypus (37.6%) and Z. koreanus (13.8%), respectively. The next most abundant species were Squalidus gracilis majimae (11.1%), Pungtungia herzi (7.7%), Rhodeus pseudosericeus (5.0%), Microphysogobio yaluensis (4.3%), and Hemibarbus longirostris (3.0%). Among the fish collected, 19 species (40.4%) were Korean endemic species. Endangered species were R. pseudosericeus and Acheilognathus signifer while exotic species were Micropterus salmoides and Oncorhynchus mykiss, and land-locked species were Plecoglossus altivelis and Rhinogobius brunneus. The community analysis showed that the dominance and evenness indexes were mostly low and that the diversity and richness indexes were high. Moreover, the river health (index of biological integrity) was mostly excllent (1 site is good, 9 sites are very good). R. pseudosericeus inhabited widely from St. 3 to St. 9 in Heuk Stream, and its population size was large. Age groups for R. pseudosericeus estimated by the frequency distribution of total length in spawning season (April) indicated the 32~45 mm group as 1-year old, the 46~59 mm group as 2-years old, 60~69 mm group as 3-years old, and the 70~89 mm group as 4-years or older. Total length range of male and female was similar, and the sex ratio of female (426) to male (394) was 1 : 0.95. A. signifer, which had been released in Heuk Stream, inhabited from St. 4 to St. 5, but its population size was small. Lastly, this paper discusses a conservation plan for fish in Heuk Stream.
Kim, Youn Hee;Lee, Gee Young;Kim, Hye Hyeong;Lee, Jae Hong;Jung, Jae Hong;Lee, Sang Deok
Journal of Plant Biotechnology
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v.46
no.1
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pp.22-31
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2019
The objective of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shooting and rooting Echeveria laui and Echeveria elegans for in vitro mass production. To determine the suitable plant parts for callus induction, the leaves were divided into upper, medium and bottom parts and cultured on MS medium at different concentrations with $0{\sim}2mgL^{-1}\;NAA$ and $0{\sim}4 mgL^{-1}BA$. The upper and middle parts of leaves both showed 100% callus formation rate with $NAA\;1\;mgL^{-1}$ and $BA\;1\;mgL^{-1}$ treatment in E. laui. The middle parts of leaves showed 83.3% callus formation rate at $NAA\;2\;mgL^{-1}$ and BA 4 mgL-1 treatment in E. elegans. The shoot induction rate from callus was highest at $NAA\;0.1\;mgL^{-1}$ and $BA\;3\;mgL^{-1}$ treatment in E. laui and $NAA\;0.3\;mgL^{-1}$ in E. elegans. In addition, the number of shoots formation was 10.4 shoots high in $NAA\;1\;mgL^{-1}$ and $BA\;1\;mgL^{-1}$ treatment in E. laui and 12.0 shoots in most effective $NAA\;1\;mgL^{-1}$ and $BA\;0.1\;mgL^{-1}$ treatment in E. elegans. In the case of acclimatization of regenerated plant, growth characteristics did not show any significant difference (35 ~ 55%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate considered plant height and appearance preference of E. laui and E. elegans. It was established that the optimization of culture condition was responsible for the mass propagation in vitro cultures of E. laui and E. elegans.
With regard to current Neck CT, Bismuth shielding boards are often being used to reduce exposure to superficial organs such as the thyroid. However, beam hardening often occurs near superficial organs with Bismuth shielding boards and variations in CT Number, Noise, and Uniformity values occur severely. This study looked into the usefulness of shielding boards made from aluminum and silicone that can be easily obtained and have good machinability by comparing them to the existing Bismuth shielding board. An Aluminum 7.3mm and a Silicone 21.5mm were made with shielding ratios similar to that of the Bismuth(0.06 mmPb). TLD (TLD-100) was placed on the thyroid area of the Phantom (RS-108T) and 5 doses were measured for each. To compare image quality, CT Number and Noise variations in axial images of the thyroid area in Neck CT images were compared. Also, variations in CT Number, Noise, and Uniformity were measured in the AAPM phantom images and compared. In the results, when thyroid doses for each shielding board were compared, the Bismuth shielding board showed a 14% reduction, the Silicone 21.5mm showed a 15% reduction, and the Aluminum 7.3mm showed a 13% reduction compared to the Non-Shield. Statistically, there were no significant differences in comparison with the Bismuth shielding board. In CT Number variations of thyroid area images, variations were largest for the Bismuth shielding board. With Uniformity evaluations of the AAPM phantom, the Bismuth shielding board was found unsuitable and the Aluminum 7.3mm and Silicone 21.5mm satisfied the acceptance criteria. Research results show that the Aluminum 7.3mm and Silicone 21.5mm have a similar shielding ratio to the high-priced Bismuth shielding board that is currently being used clinically and in comparison tests of CT Number attenuation coefficient variations, Noise, and Uniformity which are phantom image evaluation items, they proved to be better than Bismuth shielding boards. If various shielding boards are made using aluminum and silicone, sized appropriately for superficial organs, it would be useful in decreasing patient doses.
Park, Jinsoo;Choi, Jinsoo;Kim, Hyunjae;Oh, Jun;Sung, Minyoung;Ahn, Joonyoung;Lee, Sangbo;Kim, Jeongho
Journal of the Korean Society of Urban Environment
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v.18
no.4
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pp.485-494
/
2018
This study aims to identify the characteristics of oxidation and chemical composition of PM in winter season, 2017 at Incheon area. The mean concentration of air pollutants were $46{\pm}22{\mu}g/m^3-PM_{10}$, $29{\pm}18{\mu}g/m^3/-PM_{2.5}$, $5{\pm}3ppb-SO_2$, $0.56{\pm}0.24ppm-CO$, $21{\pm}13ppb-O_3$ and $28{\pm}17ppb-NO_2$, respectively. The dominant ion of the $PM_{1.0}$ chemical component were organic with $3.2{\mu}g/m^3$ and nitrate with $1.9{\mu}g/m^3$. The day and night variation of the $PM_{1.0}$ chemical components was higher in nighttime than those of daytime. The averaged nitrate oxidation rate (SOR) was 0.06 and sulfate oxidation rate was 0.11 during the field campaign. In the high mass loading period, nitrate oxidation rate (NOR) was up to 0.6 and also the nitrate in $PM_{1.0}$ was increased. The averaged ratio of $NO_x/SO_2$ was 8.7 and nitrate/sulfate was 3.1, respectively. In this results, the nitrate component in $PM_{1.0}$ was influenced by NOx from the stationary source as power plant and the mobile source around the measurement site.
We investigated the effect of seedling age and planting density on early growth performance of Zelkova serrata trees. Containerized Z. serrata seedlings, 1-year-old and 2-year-old, were planted according to two different planting densities (3,000 and 5,000 trees ha-1) at the end of March, 2017. Three plots were established for each treatment (2 seedling ages × 2 planting densities). We calculated the survival rate (%) of out-planted seedlings as well as the stem volume based on the root collar diameter (RCD) and height (H) in September of each year, from 2017 to 2019. Seedling age and planting density did not affect survival rate of the out-planted trees. Planting density did not affect height of the trees, whereas the treatment significantly affected RCD and the H/D ratio for 2-year-old seedlings in 2019. The stem volume per tree was highest in the plot of 2-year-old seedlings at 5,000 trees ha-1 and lowest in the 1-year-old seedlings at 3,000 trees ha-1. In addition, the tree height in both 1-year-old and 2-year-old Z. serrata trees was greater in the 5,000 trees ha-1 plot than in the 3,000 trees ha-1 plot. There was no interactive effect between seedling age and planting density on stem volume; however, as seedling age and planting density increased, stem volume of Z. serrata trees increased. The values for stem volume per hectare are 85%, 68%, and 300% higher in 1-year-old 5,000 trees ha-1, 2-year-old 3,000 trees ha-1, and 2-year-old 5,000 trees ha-1 plots, respectively, compared to the values in 1-year-old 3,000 trees ha-1. This result shows that high planting density (close planting) affects tree growth differently based on seedling age, and suggests that the planting density of 5,000 trees ha-1 is suitable for 2-year-old seedlings to improve early growth field performance.
This study was conducted to analyze stem decay in Quercus mongolica and Quercus variabilis in Korea. To ensure even allocation, a total of 5,005 sample trees (2,504 Q. mongolica and 2,501 Q. variabilis) were cut and collected in five regions and 27 subregions. The trees were then examined for stump decay and assigned to four classes based on the degree of scar, tissue decay and decolorization, splitting, and tree hollowing. The results show that the decay rate of Q. mongolica was 66.1%, at least twice as high as that of Q. variabilis, which was rated at 35% (χ2 = 631.15, p < 0.001). The comparison among regions indicated that the highest ratio of Q. mongolica occurs in the Central Regional Forest Service zone (76.5%), followed by the Northern zone (74.8%) and Eastern zone (65.7%). In contrast, the greatest proportion of Q. variabilis is found in the Northern Regional Forest Service zone (38.6%), followed by the Southern (32.9%) and Eastern (37.8%) zones. A statistically significant difference was seen among the five zones (p < 0.05, p < 0.001). There was also a clear tendency for the proportions for the two species to increase with a rise in the DBH. With respect to age, however, a statistically significant difference was found (p < 0.01, p < 0.05) only in Q. mongolica, whose rate increased with the increase in age. Our results show that as the DBH and age increases, the conditions of tissue decay and decolorization are manifested in Q. mongolica, whereas scars are common in Q. variabilis.
Seong, Deok-Gyeong;Kim, Young-Gwang;Cho, Yong-Cho;Kim, Min Chul;Kim, Cheol Soo;Kim, Dong-Kwan;Chung, Jung-Sung
Journal of agriculture & life science
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v.51
no.3
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pp.19-27
/
2017
This study was conducted to investigate the optimum transplanting method for increasing the rice yield and quality by early transplanting cultivation in Gyeongnam plain area. This examination used of the transplanting method of planting distance($30{\times}14$ and $30{\times}18cm$) with number of seedlings(3, 5 and 7 plants). In high planting density($30{\times}14cm$), the number of tillers was increased while plant height was decreased as the planting number increases. On the other hand, the plant height and number of tillers were increased as planting number increases in low planting density($30{\times}18cm$). However, the number of grain per panicle was increased while the number of panicle was decreased as the planting number decreases. The number of panicle and the number of grain per panicle was increased as the planting distance increase. The yield of rice, when transplanted in the distance of $30{\times}14cm$ with 3 plants, was higher than the other treatment. Head rice ratio and head rice yield was the highest when transplanted in the distance of $30{\times}18cm$ with 3 plants(403 kg/10 a). The protein content was decreased and Toyo taste value was improved when transplanted in the distance of $30{\times}18cm$. Taken together, the best transplanting method for early transplanting cultivation was low planting density($30{\times}18cm$) with 3 plants in Gyeongnam plain area.
Kim, Jae Kyung;Jang, Dong Cheol;Kang, Ho Min;Nam, Ki Jung;Lee, Mun Haeng;Na, Jong Kuk;Choi, Ki Young
Journal of Bio-Environment Control
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v.30
no.1
/
pp.1-9
/
2021
This study was conducted to examine the changes of photosynthesis, growth, chlorophyll contents and functional material contents in light intensity and EC concentration of wild baby leaf vegetable, Indian lettuce (Lactuca indica L. cv. 'Sunhyang') in DFT hydroponics. The cultivation environment was 25±1℃ of temperature and 60±5% of relative humidity in growth system. At 14 days after sowing, combination effect of light intensity (Photosynthetic Photon Flux Density (PPFD 100, 250, 500 µmol·m-2·s-1) and EC level (EC 0.8, 1.4, 2.0 dS·m-1) of nutrient solution was determined at the baby leaf stage. The photosynthesis rate, stomatal conductance, transpiration rate and water use efficiency of Indian lettuce increased as the light intensity increased. The photosynthesis rate and water use efficiency were highest in PPFD 500-EC 1.4 and PPFD 500-EC 2.0 treatment. The chlorophyll content decreased as the light intensity increased, but chlorophyll a/b ratio increased. Leaf water content and specific leaf area decreased as light intensity increased and a negative correlation (p < 0.001) was recognized. Plant height was the longest in PPFD 100-EC 0.8 and leaf number, fresh weight and dry weight were the highest in PPFD 500-EC 2.0. Anthocyanin and total phenolic compounds were the highest in PPFD 500-EC 1.4 and 2.0 treatment, and antioxidant scavenging ability (DPPH) was high in PPFD 250 and 500 treatments. Considering the growth and functional material contents, the proper light intensity and EC level for hydroponic cultivation of Indian lettuce is PPFD 500-EC 2.0, and PPFD 100 and 250, which are low light conditions, EC 0.8 is suitable for growth.
Methanol synthesized from renewable hydrogen and captured CO2 has recently attracted great interest as a sustainable energy carrier for large-scale renewable energy storage. In this study, molten carbonate fuel cell's performance was investigated with the direct conversion of methanol into syngas inside the anode chamber of the cell. The internal reforming of methanol may significantly improve system efficiency since the heat generated from the electrochemical reaction can be used directly for the endothermic reforming reaction. The porous Ni-10 wt%Cr anode was sufficient for the methanol steam reforming reaction under the fuel cell operating condition. The direct supply of methanol into the anode chamber resulted in somewhat lower cell performance, especially at high current density. Recycling of the product gas into the anode gas inlet significantly improved the cell performance. The analysis based on material balance revealed that, with increasing current density and gas recycling ratio, the methanol steam reforming reaction rate likewise increased. A methanol conversion more significant than 90% was achieved with gas recycling. The results showed the feasibility of electricity and syngas co-production using the molten carbonate fuel cell. Further research is needed to optimize the fuel cell operating conditions for simultaneous production of electricity and syngas, considering both material and energy balances in the fuel cell.
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