• 한국산학기술학회논문지
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• 제21권11호
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• pp.554-560
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• 2020

평판 디스플레이 제조 공정에서 대상물의 위치 결정을 위해 고정밀 평면 모션 스테이지를 사용한다. 이 유형의 스테이지는 일반적으로 마찰이 없는 선형 모터와 에어 베어링을 사용하며, 고정밀 위치 센서로 레이저 간섭계를 사용한다. 스테이지의 불가피한 기생 운동에 의해 야기되는 요 모션 오차는 위치 결정 대상체의 향 변화를 의미하므로, 스테이지의 성능과 공정 정밀도 향상을 위해 요 모션 오차의 실시간 동적 보정은 매우 중요하다. 요 모션 오차 보상에는 갠트리 제어가 일반적이며, 이 방법을 공기베어링 가이드를 사용하는 스테이지에 적용하기 위해서 회전 모션을 허용하는 유연기구가 스테이지에 적용된다. 본 논문은 공기베어링과 유연기구를 갖춘 H형 XY 스테이지의 정속 구동 성능을 개선하는 방법을 제안한다. 유연기구를 포함한 스테이지의 갠트리 제어 시 선형 모터로부터 발생하는 상호 리플의 발생 원인을 분석하고, 이러한 상호 리플을 보상하는 방안으로 적응 학습 제어를 제시한다. 제시 방안의 검증을 위해 시뮬레이션을 수행하여, 보상 제어를 통해 속도 리플이 약 22 % 수준으로 감소함을 확인하였다. 그리고 요 모션 오차가 발생하는 스테이지 상태를 가정하여 리플 저감 효과를 검증하였다.

• Reproductive and Developmental Biology
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• 제29권2호
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• pp.83-91
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• 2005

Somatic cell nuclear transfer in cattle has limited efficiency in terms of production of live offspring due to high incidence of fetal failure after embryo transfer to recipients. Such low efficiency of cloning could possibly arise from abnormal and poorly developed placenta. In the present study the placental proteome in late pregnancy established from in vitro fertilization (IVF) and nuclear transfer (NT) was analysed. Proteome alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF). Comparing placenta from NT embryos to those from IVF counterparts, significant changes in expression level were found in 18 proteins. Of these proteins 12 were not expressed in NT placenta but expressed in IVF counterpart, whereas the expression of the other 6 proteins was limited only in NT placenta. Among these proteins, cytokeratin 8 and vimentin are considered to be involved in regulation of post-implantation development. In particular, cytokeratin 8 and vimentin may be used as makers for placental development during pregnancy because their expression levels changed considerably in NT placental tissue compared with its IVF counterpart. Data from 2-DE suggest that protein expression was disorientated in late pregnancy from NT, but this distortion was eliminated with progression of pregnancy. These findings demonstrate abnormal placental development during late pregnancy from NT and suggest that alterations of specific placental protein expression may be involved in abnormal function of placenta.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1239-1246
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• 2016

The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development.

• 재활간호학회지
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• 제1권1호
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• pp.51-60
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• 1998

The result of studying papers about management of lower back pain published in domestic and foreign nursing and medical magazines in these 10 years is as follows ; 1. General characteristic of lower back pain ; 1) In sex distribution, there were more men than women in 5 papers and more women than men in 4 papers among 9 papers surveryed. 2) In age distribution, thirties to forties of patients had more lower back pain as surveyed by general hospitals and fifties to sixties of patients had more lower back pain as surveyed by oriental medical hospitals. 3) In cause factor, there were 50 to 65% of sprain, 32 to 44% of herniated intervertebral disc and 13 to 29% of degenerative changes. 4) In symtom distribution, there were 26 to 57% of lower back pain, 42 to 65% of lower back pain with radiating pain and 34 to 99% of paravertevral muscle spasm. 5) In period of pain management distribution, 18 to 40% of patients experienced pain for less than 6 months and 59 to 82% of them experienced pain for more than 6 months in 3 papers among 4 papers. 6) In surveying the treatment, 66 to 88% of patients had conservative treatment and there were treatments of general hospital, oriental medicine, self remedy and traditional practice in conservative treatment. 7) In job distribution, 12 to 50% of them were housekeepers, 23 to 31% office workers, 4.6 to 36% blue color workers and 11 to 15% students. 2. As psychological character lower back pain paients had anxiety, depression, anger-hostility, phobic anxiety, neurasthenia, hypochondriasis, and interpersonal sensitivity. 3. To distinguish the cause of lower back pain, plain lumbar roentgenogram, straight leg rasing test, eletromyelogic findings, somatosensory evoked potentials CT and MRI were performed. 4. To relieve lower back pain. epidural adhesiolysis, epidulal injection of local anesthetic in mixture with steroid, lumbar spinal root block, low level laser therapy, acupuncture like transcutaneous nerve stimulation(AL TENS), topical capsaicin and lumbar orthotics were used in medical field, and relaxation technique was used in nursing field. 5. Mckenzie's extension exercise and William's flexsion exercise for lower back pain were used in medical field and Yoga exercise was applied in nursing field. 6. The more school education and self efficacy were high, the better they had active coping lower back pain positively and the less self efficacy was the more they had serious pain. As a result of studying the paper there have been very little research for lower back pain in nursing fields of Korea and foreign countries. Because 60 to 80% of population expeience lower back pain at least more than once, it is necessary to develop the study and clinical practice for management of lower back pain.

• 대한공간정보학회지
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• 제22권2호
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• pp.25-32
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• 2014

하천의 체계적 관리와 계획을 위해서는 정확도 높은 측량자료가 요구되며, 이를 통해 재해예방과 환경보호, 주요 인프라인 댐이나 보 같은 하천의 관리가 가능해 진다. 본 연구는 재난관리, 건설산업의 지원 등에 다양하게 활용되는 하천측량을 현재의 토털스테이션을 이용한 측량 방법과 최근 들어 활발히 이용되고 있는 항공라이다측량 방법을 이용하여 동일지역의 데이터를 취득하고 그 성과를 비교 분석하는데 목적을 두었으며, 실험 대상지로는 강원도 북한강 상류지역을 선정 하였다. 선정된 실험 지역을 대상으로 총 2개의 횡단면도를 추출하였으며, 추출된 횡단면도를 상호 비교 분석하였다. 분석 결과 일반적인 육지부에서는 두 자료간 수준고의 평균제곱근 오차가 0.017m로 그 차이가 거의 없었으나 수직위치만 변하는 특이점이나 수심부에서는 항공라이다측량이 해당 지점의 수준고를 정확하게 측정하지 못하는 것을 알 수 있었다. 본 연구를 통해 도출된 문제점을 극복하기 위해서는 향후 지속적인 연구와 실험으로 항공라이다측량을 이용한 세부 시설물의 정확한 측정 방안이나 수심부를 투과하여 하천 바닥면을 육지부와 동일한 오차범위 내로 측정할 수 있는 대안이 마련되어야 할 것으로 보이며, 실용화 단계가 될 시점에서는 대규모 지역이나 사람의 접근이 불능한 지역에 대하여 하천뿐만 아니라 각종 엔지니어링 사업분야에서도 계획 및 설계 등 의사결정 수단으로 다양한 활용이 기대된다.

• 한국재료학회지
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• 제27권12호
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• pp.705-709
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• 2017

The electronic and optical characteristics of molybdenum disulphide ($MoS_2$) film significantly vary with its thickness, and thus a rapid and accurate estimation of the number of $MoS_2$ layers is critical in practical applications as well as in basic researches. Various existing methods are currently available for the thickness measurement, but each has drawbacks. Transmission electron microscopy allows actual counting of the $MoS_2$ layers, but is very complicated and requires destructive processing of the sample to the point where it will no longer be useable after characterization. Atomic force microscopy, particularly when operated in the tapping mode, is likewise time-consuming and suffers from certain anomalies caused by an improperly chosen set point, that is, free amplitude in air for the cantilever. Raman spectroscopy is a quick characterization method for identifying one to a few layers, but the laser irradiation causes structural degradation of the $MoS_2$. Optical microscopy works only when $MoS_2$ is on a silicon substrate covered with $SiO_2$ of 100~300 nm thickness. The last two optical methods are commonly limited in resolution to the micrometer range due to the diffraction limits of light. We report here a method of measuring the distribution of the number of $MoS_2$ layers using a low voltage field emission electron microscope with acceleration voltages no greater than 1 kV. We found a linear relationship between the FESEM contrast and the number of $MoS_2$ layers. This method can be used to characterize $MoS_2$ samples at nanometer-level spatial resolution, which is below the limits of other methods.

• 공업화학
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• 제31권2호
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• pp.220-225
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• 2020

모발은 여러 가지 아미노산들을 포함하는 단백질로 이루어져 있다. 자외선(UV)은 태양광선중에서 모발손상에 가장 큰 영향을 미치며 모발 노화에 주된 역할을 한다. 본 연구의 목적은 전자현미경(SEM), 공초점현미경(CLSM) 및 적외선 현미경분광법(IR micro spectroscopy)을 이용하여 정상모발에 UVB를 조사한 후 특징적인 형태학적 및 화학적 구조변화를 알아보는 것이다. 에너지 분산형 X선 분광기가 부착된 전자현미경은 자외선 조사모발의 표면이 정상모발과 비교했을 때 거칠고 높은 산소원소의 함량을 보였다. 형광 및 3차원 위상 이미지를 CLSM으로 분석한 결과 정상모발의 초록색 형광방출이 UVB 조사모발에 비해 매우 높았다. 또한 fluorescamine 형광 염색법을 통해 UVB 조사모발은 정상모발에 비해 펩타이드 결합의 파괴로 생성된 자유 아미노기가 많음을 확인할 수 있었다. UVB 조사모발의 강한 푸른색 형광은 아미노기의 함량이 높다는 것을 의미하며, 이는 CLSM에서도 관찰되었다. 따라서 fluorescamine은 UVB 조사모발에서 펩타이드 결합의 파괴를 관찰하는데 유용한 도구가 될 수 있다. 정상모발과 UVB 조사모발의 단면을 IR micro-spectroscopy를 통해 이미지 맵핑(mapping)한 결과, UVB 조사모발은 정상 모발에 비해 모발의 표면은 물론 내부에 걸쳐 디설파이드 결합(disulfide bond)의 산화가 일어나고 있음을 확인할 수 있었다. 이러한 분광학적 방법은 단독 또는 다른 분석법과 함께 모발화장품의 개발에 응용될 수 있을 것이다.