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Characterization of Placental Proteins in Bovine Somatic Cell Clone Fetuses  

Woo, Jei-Hyun (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration, Genetic Engineering Laboratory, Department, Genetic Engineering, Sungkyunkwan University)
Ko, Yeoung-Gyu (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Kim, Bong-Ki (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Kim, Jong-Mu (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Lee, Youn-Su (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Kim, Nam-Yun (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Im, Gi-Sun (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Yang, Boung-Chul (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Seong, Hwan-Hoo (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Jung, Jin-Kwan (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Kwun, Moo-Sik (Genetic Engineering Laboratory, Department, Genetic Engineering, Sungkyunkwan University)
Chung, Hak-Jae (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
Publication Information
Reproductive and Developmental Biology / v.29, no.2, 2005 , pp. 83-91 More about this Journal
Abstract
Somatic cell nuclear transfer in cattle has limited efficiency in terms of production of live offspring due to high incidence of fetal failure after embryo transfer to recipients. Such low efficiency of cloning could possibly arise from abnormal and poorly developed placenta. In the present study the placental proteome in late pregnancy established from in vitro fertilization (IVF) and nuclear transfer (NT) was analysed. Proteome alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF). Comparing placenta from NT embryos to those from IVF counterparts, significant changes in expression level were found in 18 proteins. Of these proteins 12 were not expressed in NT placenta but expressed in IVF counterpart, whereas the expression of the other 6 proteins was limited only in NT placenta. Among these proteins, cytokeratin 8 and vimentin are considered to be involved in regulation of post-implantation development. In particular, cytokeratin 8 and vimentin may be used as makers for placental development during pregnancy because their expression levels changed considerably in NT placental tissue compared with its IVF counterpart. Data from 2-DE suggest that protein expression was disorientated in late pregnancy from NT, but this distortion was eliminated with progression of pregnancy. These findings demonstrate abnormal placental development during late pregnancy from NT and suggest that alterations of specific placental protein expression may be involved in abnormal function of placenta.
Keywords
Cloned cow; 2-DE; Placentome; Vimentin; Cytokeratin 8; Aldose reductase; PRP-1;
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