• KSBB Journal
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• v.15 no.1
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• pp.14-21
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• 2000

In this study, algae cultivation using the photobioreactor has been applied to remove the nitrogen and phosphorus compounds in the wastewater of the livestock industry. The optimal ratio of nitrate and ortho-phosphate concentration was found for the enhancement of removal efficiency. To achieve the high density culture of algae, the photobioreactor consisted of optical fibers wes developed to get the sufficient light intensity. The light could be illuminated uniformly from light source to the entire reactor by the optical fibers. The structured kinetic model was proposed to describe the growth rate, consumption rate of nitrates and ortho-phosphates in algae culture. The self-organizing fuzzy logic controller incorporated with genetic algorithm was constructed to control the semi-continuous wastewater treatment system. The proposed fuzzy logic controller was applied to maintain the nitrated concentration at the given set-point with the control of wastewater feeding rate. The experimental results showed that the self-organizing fuzzy logic controller could keep the nitrate concentration and enhance algae growth.

• Microbiology and Biotechnology Letters
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• v.17 no.3
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• pp.257-262
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• 1989

The induction of calli from tissues of a grape, Vitis hybrid, and their suspension cultures were performed and various factors were investigated on cell growth and anthocyanin production. It was shown that light intensity and inorganic nitrogen concentration played an important role on anthocyanin production.1:he contents of anthocyanin produced under 10,000 Iux light irradiation were about twice as much as under the dark. The reduction of inorganic nitrogen concentration of MS medium to one to twenty brought about the increase of approximately five to six-fold in total anthocyanin or sixteenfold in anthocyanin content per dry cell weight and addition of nitrate only as inorganic nitrogen source was shown to be the best for anthocyanin production. Miller medium and Gamborg medium were suitable for the anthocyanin production, as well as high concentrations of Co$^{2+}$ and Fe$^{2+}$. And high yield of 40mg anthocyanins per 200m1 flask was obtained by two stage culture using MS medium for the first stage and the modified MS medium for the second stage.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2019.05a
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• pp.278-280
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• 2019

Flow cytometer is one of the instrument that can measure various optical properties of a single cell or microparticle. These parameters including size, granularity, and fluorescence intensity are determined by the physical and optical interaction of the cells with excitation light source. However, users have some difficulties such as high cost, size of instrument, and limited fluorescence selectivity. In addition, abundant data is also unintentionally acquired even though user wants to have a single optical parameter. For these reasons, the use of flow cytometer is more challenging for researchers to apply their study. Therefore, the proposed study aims to develop a low-cost portable fluorescence acquisition system using a commercially available light-emitting diode and photodiode. It is designed by a 3D printer, and fluorescence selectivities are increased by changing of the light source / optical filter / detection sensor. Various number sets of fluorescently labeled cells were measured, and its feasibility was evaluated through the proposed system. As a result, acquried fluorescence intensities were proportional to the concentration of the cells and showed high linearity.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.07a
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• pp.237-242
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• 2001

The stochiometric mix of evaporating materials for the CuGaSe$_2$ single crystal thin films was prepared from horizontal furnace. To obtain the single crystal thin films, CuGaSe$_2$ mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were 610$^{\circ}C$ and 450$^{\circ}C$, respectively The crystalline structure of single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). From the photocurrent spectrum by illumination of perpendicular light on the c-axis of the CuGaSe$_2$ single crystal thin film, we have found that the values of spin orbit splitting Δ So and the crystal field splitting ΔCr were 91 meV and 249.8 meV at 20 K, respectively. From the Photoluminescence measurement on CuGaSe$_2$ single crystal thin film, we observed free excition (Ex) existing only high quality crystal and neutral bound exiciton (D$^{\circ}$,X) having very strong peak intensity. Then, the full-width-at-half-maximum(FWHM) and binding energy 7f neutral acceptor bound excision were 8 meV and 35.2 meV, respectivity. By Haynes rule, an activation energy of impurity was 355.2 meV

• Microbiology and Biotechnology Letters
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• v.29 no.4
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• pp.227-233
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• 2001

To maximize astaxanthin (3,3'-dihydroxy-$\beta\beta$'carotene-44'-dione) production by high density Haematococcus pluvialis cultures, various, media were examined Among tested media, \`Hong Kong Medium and Modified Bolds Basal Medium showed the best result for cell growth ( $2.0$\times$10^{ 6}$cells /mL) and for astaxanthin content per cell (9.7 mg astaxanthin mg/g cell), respectively, Maximum astasanthin concentration of 6.1mL was obtained at pH 7.5, $20^{\circ}C$~$25^{\circ}C$ Deficiencies of nitrogen source($NaNO_3$ and proteose-peptone) found to simulate astaxanthin formation Relatively low light inten- sity of $60\mu$E ($\m^2$s) was sutiable for vegetative cell growth while higher light intensity was required for higher astaxanthin accumulation.

• Journal of the Korea Institute of Military Science and Technology
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• v.17 no.1
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• pp.90-95
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• 2014

The system design and the system performance analysis of 3D imaging laser radar system for the mapping purpose is addressed in this article. For the mapping, a push-bloom scanning method is utilized. The pulsed fiber laser with high pulse energy and high pulse repetition rate is used for the light source of laser radar system. The high sensitive linear mode InGaAs avalanche photo-diode is used for the laser receiver module. The time-of-flight of laser pulse from the laser to the receiver is calculated by using high speed FPGA based signal processing board. To reduce the walk error of laser pulse regardless of the intensity differences between pulses, the time of flight is measured from peak to peak of laser pulses. To get 3D image with a single pixel detector, Risley scanner which stirs the laser beam in an ellipsoidal pattern is used. The system laser energy budget characteristics is modeled using LADAR equation, from which the system performances such as the pulse detection probability, false alarm and etc. are analyzed and predicted. The test results of the system performances are acquired and compared with the predicted system performance. According to test results, all the system requirements are satisfied. The 3D image which was acquired by using the laser radar system is also presented in this article.

• Proceedings of the KSRS Conference
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• 2008.10a
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• pp.344-347
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• 2008

Ocean surface waves may be modified by ocean current and their observation may be severely distorted if the observer is on a moving platform with changing speed. Tidal current near a sill varies inversely with the water depth, and results spatially inhomogeneous modulation on the surface waves near the sill. For waves propagating upstream, they will encounter stronger current before reaching the sill, and therefore, they will shorten their wavelength with frequency unchanged, increase its amplitude, and it may break if the wave height is larger than 1/7 of the wavelength. These small scale (${\sim}$ 1 km changes is not suitable for satellite radar observation. Spatial distribution of wave-height spectra S(x, y) can not be acquired from wave gauges that are designed for collecting 2-D wave spectra at fixed locations, nor from satellite radar image which is more suitable for observing long swells. Optical images collected from cameras on-board a ship, over high-ground, or onboard an unmanned auto-piloting vehicle (UAV) may have pixel size that is small enough to resolve decimeter-scale short gravity waves. If diffuse sky light is the only source of lighting and it is uniform in camera-viewing directions, then the image intensity is proportional to the surface reflectance R(x, y) of diffuse light, and R is directly related to the surface slope. The slope spectrum and wave-height spectra S(x, y) may then be derived from R(x, y). The results are compared with the in situ measurement of wave spectra over Keelung Sill from a research vessel. The application of this method is for analysis and interpretation of satellite images on studies of current and wave interaction that often require fine scale information of wave-height spectra S(x, y) that changes dynamically with time and space.

• KSBB Journal
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• v.17 no.2
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• pp.158-161
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• 2002

In this study, a prototype fiber-optic biosensor was fabricated using the inhibition of enzyme reaction by organophosphorus compounds to detect organophosphorus compounds, which is nervous toxic material an? is used as chemical weapon and pesticide. Enzyme, substrate, and inhibitor for enzyme reaction were acetylcholinesterase (key enzyme in nervous cell), acetylthiocholine iodide, and paraoxon (a kind of organophosphorus compounds), respectively. The detection principle of sensor is the detection of enzyme reaction inhibited by organophosphorus compounds by the quantitative measurement of acetic acid, which was achieved by absorbance measurement using litmus solution that maximum absorbance band is changed by pH. To fabricate prototype fiber-optic biosensor, high bright LED and photodiode was used as light source and light intensity detector, respectively. From the experimental results using a prototype biosensor, the linear change of sensor signal was obtained in a range of 0-2 ppm inhibitor concentrations. From these results, it was verified that the quantitative measurement of organophosphorus compounds could be achieved fast (within 2 minutes) and accurately by a prototype fiber-optic biosensor.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.11a
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• pp.171-174
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• 2004

The stochiometric $AgGaSe_2$ polycrystalline mixture of evaporating materials for the $AgGaSe_2$ single crystal thin film was prepared from horizontal furnace. To obtain the single crystal thin films, $AgGaSe_2$ mixed crystal and semi-insulating GaAs(100) wafer were used as source material and substrate for the Hot Wall Epitaxy (HWE) system, respectively. The source and substrate temperature were fixed at $630^{\circ}C$ and $420^{\circ}C$, respectively. The thickness of grown single crystal thin films is $2.1{\mu}m$. The single crystal thin films were investigated by photoluminescence and double crystal X-ray diffraction(DCXD) measurement. The carrier density and mobility of $AgGaSe_2$ single crystal thin films measured from Hall effect by van der Pauw method are $4.89{\times}10^{17}\;cm^{-3},\;129cm^2/V{\cdot}s$ at 293K, respectively. From the photocurrent spectrum by illumination of perpendicular light on the c - axis of the $AgGaSe_2$ single crystal thin film, we have found that the values of spin orbit splitting ${\Delta}S_o$ and the crystal field splitting ${\Delta}C_r$ were 0.1762 eV and 0.2494 eV at 10 K, respectively. From the photoluminescence measurement of $AgGaSe_2$ single crystal thin film, we observed free excition $(E_X)$ observable only in high quality crystal and neutral bound exciton $(D^o,X)$ having very strong peak intensity And, the full width at half maximum and binding energy of neutral donor bound excition were 8 meV and 14.1 meV, respectively. By Haynes rule, an activation energy of impurity was 141 meV.

• Journal of Environmental Science International
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• v.9 no.1
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• pp.1-11
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• 2000

A study on the variational characteristics of water quality and phytoplankton biomass by principal component analysis(PCA) was carried out in Kogum-sudo from February to October in 1993. We analyzed PCA on biological factors such as chlorophyll a and phytoplankton cell numbers for centric and pennate diatoms, phytoflagellates, and total phytoplankton as well as physico-chemical factors as water temperature, salinity, transparency, dissolved oxygen(DO), saturation of DO, apparent oxygen utilization (AOU), chemical oxygen demand(COD), nutrient (ammonia, nitrite, nitrate, phosphate and silicate), N/P ratio and suspended solid(SS). The source of nutrients supply depended on the mineralization of organic matters and inputs of seawater from outside rather than runoff of freshwater. The phytoplankton biomass was changed within short interval period by nutrients change. And it was controlled by the combination of several environmental factors, especially of light intensity, ammonia and phosphate. The marine environmental characteristics were determined by the mineralization of organic matters in winter, by runoff of freshwater including high nutrients concentration in spring, by ammonia uptake and high phytoplankton productivity in summer, and phosphate supplied input seawater from outside of Kogeum-sudo in autumn. And Kogum-sudo was separated with 2 regions by score distributions of PCA. That is to say, one region was middle parts of straits which was characterized by the mixing seawater and the accumulated organic matters, other one region was Pungnam Bay and the water around Kogum Island which was done by high phytoplankyon biomass and productivity year-round.