• 한국항공우주학회지
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• 제47권10호
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• pp.720-727
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• 2019

하이브리드 로켓 연소의 저주파수 연소불안정은 고체연료의 열적지연(Thermal Lag)과 경계층 유동 변화에 의한 열전달 진동의 공진에 의해 발생한다. 본 연구는 연료 표면 근처의 경계층 유동의 교란이 어떤 물리적 과정에 의해 발생하여 연소불안정으로 발달하는지를 실험적으로 확인하였다. 특히 산화제의 스월 분사는 연소 안정화에 매우 큰 기여를 하므로 스월 강도를 증가시키며 경계층의 변화와 연소불안정의 발생과정을 연구하였다. 경계층 섭동을 확인하기 위하여 연소 유동장을 가시화하였고 이미지에 대한 POD(Proper Orthogonal Decomposition) 분석을 시도하였다. 스월 강도가 증가할수록 500Hz 대역 고주파수 p', q'의 결합이 약해지며 열적지연과 유사한 주파수 특성을 갖는 Rayleigh Index의 섭동 발생도 약해져 경계층 진동의 발생이 점차 감소하는 것을 관찰하였다. 따라서 고주파수 p', q'의 주기적인 결합에 의한 축 방향 경계층 진동이 나타나면 열적지연 주파수와 공진에 의한 연소불안정이 발생함을 확인하였다.

• Journal of Biosystems Engineering
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• 제39권2호
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• pp.111-114
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• 2014

Purpose: This review aims to introduce aptamers and the methods of its development to improve the sensitivity and selectivity to target bacteria. In this review, we have highlighted current developments and directions in the pathogen detection based on aptamers. Background: Aptamers, the specific nucleic acid sequences, can bind to targets with high affinity and specificity. Some of researches on the use of aptamers for the detection of pathogen have been reported in recent years. Aptamers have more applicability than antibodies for the development of pathogen detection using biosensor; such as easy to synthesis and labeling, lack of immunogenicity, and a low cost of production. However, only few reports on the development and use of aptamers for the detection of pathogen have been published. Review: Aptamers specific to pathogen are obtained by whole-cell systematic evolution of ligands by exponential enrichment (SELEX) process. SELEX process is composed of screening random oligonucleotide bound with target cells, multiple separation and amplification of nucleic acids, final identification of the best sequences. For improving those affinity and selectivity to target bacteria, optimization of multiple separating process to remove unbounded oligonucleotides from aptamer candidates and sorting process by flow cytometry are required.

• 한국가시화정보학회지
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• 제14권1호
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• pp.57-61
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• 2016

We report a finding of fast(exceeding 2,000 K/s) heating of polydimethylsiloxane(PDMS), one of the most commonly-used microchannel materials, under cyclic loadings at high(~MHz) frequencies. A microheater was created based on the finding. The heating mechanism utilized vibration damping of sound waves, which were generated and precisely manipulated using a conventional surface acoustic wave(SAW) microfluidic system, in PDMS. The penetration depths were measured to range from $210{\mu}m$ to $1290{\mu}m$, enough to cover most microchannel heights in microfluidic systems. The energy conversion efficiency was SAW frequency-dependent and measured to be the highest at around 30 MHz. Independent actuation of each interdigital transducer(IDT) enabled independent manipulation of SAWs, permitting spatiotemporal control of temperature on the microchip. All the advantages of this microheater facilitated a two-step continuous flow polymerase chain reaction(CFPCR) to achieve the billion-fold amplification of a 134 bp DNA amplicon in less than 3 min. In addition, a technique was developed for establishing dynamic free-form temperature gradients(TGs) in PDMS as well as in gases in contact with the PDMS.

• 한국항공우주학회지
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• 제50권11호
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• pp.783-789
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• 2022

최근 연구에 의하면 하이브리드 로켓의 후연소실로 유입되는 연소유동은 고주파수 와류흘림을 포함하고 있으며, 노즐 벽면과 충돌하여 대향류가 형성되며 점화지연을 동반한 추가적인 연소가 발생하는 것이 확인되었다. 본 연구는 대향류 발생에 의한 점화지연이 연소압력 맥놀이가 나타나는 원인임을 확인하려 한다. 이를 위하여 Culick이 제안한 기존의 열음향 불안정 발생에 대한 에너지 킥 모델에 점화지연 발생을 반영한 수정 모델을 제안하였고 수치계산을 통하여 점화지연의 크기 변화가 열음향 결합에 의한 연소압력 맥놀이 발생을 결정하는 중요한 인자임을 확인하였다. 또한 후연소실 길이가 증가함에 따라 실험에서 관찰된 점화지연 감소는 에너지 킥과 압력의 위상 차의 증가를 가져와 맥놀이현상인 주기적인 압력증폭이 전혀 나타나지 않는 것도 확인하였다.

• 실내환경 및 냄새 학회지
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• 제17권4호
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• pp.355-361
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• 2018

Mold grows more easily when humidity is higher in indoor spaces, and as such is found more often on wetted areas in housing such as walls, toilets, kitchens, and poorly managed spaces. However, there have been few studies that have specifically assessed the level of mold in the indoor spaces of water-damaged housing in the Republic of Korea. We investigated the levels of airborne mold according to the characteristics of water damage types and explored the correlation between the distribution of mold genera and the characteristics of households. Samplings were performed from January 2016 to June 2018 in 97 housing units with water leakage or condensation, or a history of flooding, and in 61 general housing units in the metropolitan and Busan area, respectively. Airborne mold was collected on MEA (Malt extract agar) at flow rate of 100 L/min for 1 min. After collection, the samples were incubated at $25^{\circ}C$ for 120 hours. The cultured samples were counted and corrected using a positive hole conversion table. The samples were then analyzed by single colony culture, DNA extraction, gene amplification, and sequencing. By type of housing, concentrations of airborne mold were highest in flooded housing, followed by water-leaked or highly condensed housings, and then general housing. In more than 50% of water-damaged housing, the level of airborne mold exceeded the guideline of Korea's Ministry of Environment ($500CFU/m^3$). Of particular concern was the fact that the I/O ratio of water-damaged housing was greater than 1, which could indicate that mold damage may occur indoors. The distribution patterns of the fungal species were as follows: Penicillium spp., Cladosporium spp. (14%), Aspergillus spp. (13%) and Alternaria spp. (3%), but significant differences of their levels in indoor spaces were not found. Our findings indicate that high levels of mold damage were found in housing with water damage, and Aspergillus flavus and Penicillium brevicompactum were more dominant in housing with high water activity. Comprehensive management of flooded or water-damaged housing is necessary to reduce fungal exposure.