Browse > Article
http://dx.doi.org/10.5407/JKSV.2016.14.1.057

Acoustothermal Heating of Polydimethylsiloxane Microfluidic Systems and its Applications  

Sung, Hyung Jin (Department of Mechanical Engineering, KAIST)
Ha, Byunghang (Department of Mechanical Engineering, KAIST)
Park, Jinsoo (Department of Mechanical Engineering, KAIST)
Destgeer, Ghulam (Department of Mechanical Engineering, KAIST)
Jung, Jin Ho (Department of Mechanical Engineering, KAIST)
Publication Information
Journal of the Korean Society of Visualization / v.14, no.1, 2016 , pp. 57-61 More about this Journal
Abstract
We report a finding of fast(exceeding 2,000 K/s) heating of polydimethylsiloxane(PDMS), one of the most commonly-used microchannel materials, under cyclic loadings at high(~MHz) frequencies. A microheater was created based on the finding. The heating mechanism utilized vibration damping of sound waves, which were generated and precisely manipulated using a conventional surface acoustic wave(SAW) microfluidic system, in PDMS. The penetration depths were measured to range from $210{\mu}m$ to $1290{\mu}m$, enough to cover most microchannel heights in microfluidic systems. The energy conversion efficiency was SAW frequency-dependent and measured to be the highest at around 30 MHz. Independent actuation of each interdigital transducer(IDT) enabled independent manipulation of SAWs, permitting spatiotemporal control of temperature on the microchip. All the advantages of this microheater facilitated a two-step continuous flow polymerase chain reaction(CFPCR) to achieve the billion-fold amplification of a 134 bp DNA amplicon in less than 3 min. In addition, a technique was developed for establishing dynamic free-form temperature gradients(TGs) in PDMS as well as in gases in contact with the PDMS.
Keywords
Acoustothermal Heating; Surface Acoustic Wave; Temperature Gradient; Continuous Flow PCR;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Miralles, V., Huerre, A., Malloggi, F. and Jullien, M.C., 2013, "A Review of Heating and Temperature Control in Microfluidic Systems: Techniques and Applications," Diagnostics, Vol.3(1), pp.33-67.   DOI
2 Chang, C.M., Chang, W.H., Wang, C.H., Wang, J.H., Mai, J.D. and Lee, G.B., 2013, "Nucleic Acid Amplification Using Microfluidic Systems," Lab Chip, Vol.13(7), pp.1225-1242.   DOI
3 Oda, R.P., Strausbauch, M.A., Huhmer, A.F.R., Borson, N., Jurrens, S.R., Craighead, J., Wettstein, P.J., Eckloff, B., Kline, B. and Landers, J.P., 1998, "Infrared-Mediated Thermocycling for Ultrafast Polymerase Chain Reaction Amplification of DNA," Anal. Chem., Vol.70(20), pp.4361-4368.   DOI
4 Fermer, C., Nilsson, P. and Larhed, M., 2003, "Microwave-Assisted High-Speed PCR," Eur. J. Pharm. Sci., Vol.18(2), pp.129-132.   DOI
5 Ha, B.H., Lee, K.S., Destgeer, G., Park, J., Choung, J.S., Jung, J.H., Shin, J.H. and Sung, H.J., 2015, "Acoustothermal Heating of Polydimethylsiloxane Microfluidic System," Sci. Rep., Vol.5, pp.11851.   DOI
6 Ha, B.H., Park, J., Destgeer, G., Jung, J.H. and Sung, H.J., 2015, "Generation of Dynamic Free-Form Temperature Gradients in a Disposable Microchip," Anal. Chem., Vol.87(22), pp.11568-11574.   DOI
7 Reichl, M., Herzog, M., Gotz, A. and Braun, D., 2014, "Why Charged Molecules Move Across a Temperature Gradient: the Role of Electric Fields. Phys. Rev. Lett., Vol.112(19), p.198101.   DOI
8 Mao, H., Yang, T. and Cremer, P.S., 2002, "A Microfluidic Device with a Linear Temperature Gradient for Parallel and Combinatorial Measurements," J. Am. Chem. Soc., Vol.124(16), pp.4432-4435.   DOI