• 한국동물위생학회지
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• 제36권1호
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• pp.45-52
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• 2013

The purpose of the present study is to determine whether Schisandra chinensis (SC) has a protective effect on high fat diet (HFD)-induced fatty liver including hepatic lipid accumulation in rats. The HFD-induced obese rats were weighed after SC extracts were administered through the gastrointestinal tract at a concentration of 250 mg/kg b.w/day for 5 weeks. After 5 weeks, all of the rats on a high fat-diet were 36.5% heavier compared with normal controls. In contrast, rats on a high-fat diet supplemented with SC were 23.5% lighter than rats fed only a high-fat diet. Although there was no significant difference in food intake among the groups during the experimental diet period, the body weight gain of the SC group was significantly lower than the weights of the HFD groups. SC treatment slightly decreased the liver weight. Reduction of hepatic TBARS contents by SC was observed in rats fed a diet containing SC, and antioxidant activity was markedly increased in HFD＋SC group compared to those of HFD group in liver. Moreover, total-lipid and triglyceride contents in the liver of groups fed a diet containing SC were significantly lower compared to those of the HFD group. High fat feeding elevated liver cholesterol concentration, but the addition of SC to the HFD rats resulted in the significant decrease in liver cholesterol. In histological observation of liver tissues, the hepatocytes of HFD rats showed a typical fatty liver morphology showing numerous lipid droplets in cytoplasm, whereas administration of SC reduced the size and numbers of lipid droplets. These results clearly demonstrated the attenuation of SC on nonalcoholic fatty liver induced by obese rats fed HFD.

• Journal of Nutrition and Health
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• 제30권9호
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• pp.1035-1044
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• 1997

There is substantial evidence that insulin sensitivity can be enhanced through appropriate dietary management . In this study, insulin sensitivity was evaluated using and insulin suppression test. Male Sprague-Dawley rats, were caused to be in a diabetic condition by the injection of streptozotocin, and divided into four groups. They were fed one of the following diets for 2 weeks : (group 1) a high-carbohydrate(CHO) low-fat low-fiber diet, (group 2) a high CHO low-fat and high-fiber diet, (group 3) a low-CHO high-fat and low-fiber diet, and (group 4) a low-CHO low-fat diet groups (as comparison between group 1 and group 2 shows). In the low-CHO high-fat diet groups, dietary fiber tended to decrease plasma glucose levels at the end of the experiment, but not significantly (as comparison between group 3 and group 4 shows). The average steady state plasma glucose level in rats on the group 3 diet was the highest among all four groups(p<0.05), indicating the poorest insulin sensitivity . However, high fiber increased insulin sensitivity in rats on the low-CHO high-fat diets(as shows by a comparison between group 3 and group 4). On the other hand , the high-CHO low-fat enhanced insulin sensitivity in rats on the low fiber diet(group 1 and group 3). The degree of enhancement of insulin sensitivity depends on the combination of CHO, fat , and fiber in the diet. In conclusion, this study demonstrates that a low-CHO high-fat low -fiber diet may be deleterious to diabetic rats. In view of insulin sensitivity enhancement , dietary fiber level is irrelevant, as long as the diet has a high-CHO and low-fat level.

• Journal of Nutrition and Health
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• 제33권4호
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• pp.386-394
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• 2000

This study was conducted to investigate the effects of shellfish hydrolysate on lipid metabolism in rats fed high fat diet. Male Sprague-Dawley rate weighting approximately 110g were fed basal control diet, high fat diet and high fat diet plus 4 different shellfish hydrolyates for 4 weeks. The shellfish hydrolysates from the different sources, were oyster, hard-shelled mussel, little neck clam and march clam. After 4 weeks, serum GOT, GPT, ${\gamma}$-GTP, triglyceride and total cholesterol was significantly decreased in shellfish hydrolysates supplementation with high fat diet compared to basal control and high-fat group(p<0.05). The total lipid and cholesterol content in liver showed significant decrease(p, 0.05). There were no different in serum GPT, HDL-cholesterol, liver total cholesterol and lipid of rats between basal control diet and high-fat diet. The unsaturated fatty acids, specific components of shellfishes were a little components in shellfish hydrolysate as they were a low and not different among the groups and were most well reflected in liver and plasma. Considering digestive and absorptive process of in human body, it was assumed that the hypolipidemic effect of shellfish was not under the influence of unsaturated fatty acids but the other components, peptides, taurine and betaine and so on was detected in the process of hyperlipidemia induced by high-fat diet.

• Journal of Nutrition and Health
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• 제42권5호
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• pp.415-422
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• 2009

본 연구에서는 포도박이 고지방식이를 섭취한 흰쥐의 효소 활성과 지질과산화 수준에 미치는 영향을 조사함으로써 포도박의 생리활성과 자원화에 필요한 기초자료를 얻고자하였다. 고지방식이를 섭취한 흰쥐에게 포도박 실험식이를 급여한 후 혈청, 간조직 중의 지질과산화물 함량, glutathione 함량과 간조직 효소 활성을 측정하였다. 포도박 첨가군의 식이섭취량은 대조군에 비하여 감소하였고, 체중증가량의 실험군 간 유의적인 차이는 없었다. 식이효율은 고지방식이군이 정상식이군 보다 유의적으로 증가하였다. 지질과산화물 함량은 혈청의 경우 정상식이군에서는 포도박 첨가에 의한 변화가 없었으나 고지방식이군에서 함량이 증가되었고 포도박 첨가에 의해 감소되었다. 간조직과 간 microsome에서는 포도박을 첨가한 군이 각각의 대조군에 비하여 유의적으로 감소하였다. 간조직 내의 총glutathione 함량과 GSH/GSSG 비는 포도박 첨가군이 대조군에 비하여 모두 유의적으로 증가하였다. 간조직의 SOD 활성은 정상식이군에서는 차이가 없었으나 고지방식이에 포도박을 첨가한 군이 고지방대조군에 비하여 활성이 유도되었다. 간조직의 catalase 활성은 대조군에 비하여 포도박 첨가군이 유의적으로 증가하였다. G6Pase 활성은 포도박의 첨가로 인하여 대조군보다 활성이 증가하였지만 실험군간 유의적인 차이는 없었다. GST와 GSH-Px 활성은 정상 식이군에서는 변화되지 않았으나 고지방식이에 포도박을 첨가한 군이 고지방대조군 보다 효소 활성이 유의적으로 증가되었다. 이상의 결과를 종합해 보면 식이섬유와 폴리페놀 성분을 다량 함유한 포도박 식이는 체내 항산화계를 활성화함으로써 지질 산화와 관련성이 높은 심혈관계 질환의 예방효과를 가져올 수 있는 것으로 기대되었다. 포도박의 이러한 생리활성에 대한 연구결과는 향후 포도 가공 중에 얻어지는 포도박 폐기물을 자원화 할 수 있는 기초자료로 이용될 것으로 사료된다.

• Food Science and Biotechnology
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• 제18권1호
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• pp.179-184
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• 2009

The aim of this study was to investigate effects of onion, red onion, or quercetin on plasma antioxidant vitamin, lipid peroxidation, and leukocyte DNA damage in rats fed a high fat-cholesterol diet. Forty SD male rats were assigned to normal control, high fat-cholesterol diet (HF), or HF+5% onion powder, HF+5% red onion powder, or HF+0.0l% quercetin. The HF diet resulted in significantly higher plasma lipid peroxidation which decreased with onion, red onion, or quercetin supplementation. Leukocyte DNA damage induced by HF diet decreased significantly in rats fed onion and red onion, while quercetin supplementation had no effect on preventing leukocyte DNA damage. $H_2O_2$ induced leukocyte DNA damage exhibited a highly significant negative correlation with plasma retinol and tocopherols. These results suggest that onion or red onion powder exerts a protective effect with regard to DNA damage in rats fed HF diet. However, 0.01% quercetin in pure form might not be effective at preventing DNA damage.

• 한국식품영양과학회지
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• 제32권6호
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• pp.893-898
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• 2003

100% 글루코만난으로 제조된 국수의 체중감소 효과를 측정하기 위해 고지방식이에 의해 유도된 비만 흰쥐에게 35일간 급여 수준을 달리하여 공급하면서 비만지수, 혈청 및 혈액 영양생화학적 분석을 실시하였다. 그 결과, 고지방식이군인 NO군이 높은 체중증가량을 보였고, N25군, N50군 순으로 체중 감소정도가 컸다. 비만지수와 체지방 함량의 변화도 같은 경향이었으며, 혈액 및 혈청 생화학적 분석으로 영양상태를 측정 한 결과, 대체적으로 국수를 25% 섭취한 N25군이 국수를 50%섭취한 N50군에 비해 영양상태가 양호하게 나타났다. 본 연구 결과, 섬유성분이 대부분인 국수는 적은 양, 적은 칼로리로도 포만감을 주어 체중감량에 효과가 크나, 섭취 비율이 다른식이와 적절할 때 영양상에도 문제가 없음을 알 수 있었다. 따라서, 글루코만난으로 제조된 식품인 ‘국수’는 다이어트 식품소재로 좋은 효과가 있는 것으로 시사되었다.

• 대한의생명과학회지
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• 제10권4호
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• pp.447-452
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• 2004

This study was designed to investigate the effect of Codonopsis lanceolata extracts on carbon tetrachloride (CCl₄)­induced hepatotoxicity in rats fed high, medium or low fat diet. The liver weight was increased not by the level of fat in diet but by administration of CCl₄. Activities of serum aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased by CCl₄, the activities of AST and alanine aminotransferase (ALT) were significantly decreased in case of CCl₄ treatment with C. lanceolata extracts. In high fat diet with CCl₄ administration, the content of total lipid in liver was significantly increased, which was significantly decreased by the administration of C. lanceolata extracts. Administration of C. lanceolata water extracts remarkably prevented the tissue injury such as lipid degeneration, infiltration of inflanunatory cells and necrosis caused by CCl₄, but those methanol extracts did not affect histopathological changes of the liver as water extract. The data suggest that intake of C. lanceolata extracts would prevent lipid accumulation and hepatotoxicity induced by high fat diet and CCl₄ abministratim, and the water extract of C. lanceolata is supposed to be more effective than those methanol extract.

• Food Science and Biotechnology
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• 제18권1호
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• pp.197-201
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• 2009

This study evaluated the effect of biocellulose and the diet formulation on reducing body weight gain of obese rats induced by high-fat diet for 10 weeks. Thirty male Sprague-Dawley rats were randomly assigned to high-fat diet group (OB-CON), high-fat diet group containing 5% biocellulose (OB-BIO), and high-fat diet group containing 5% dietary formulation (OB-DF). After feeding each diet for additional 10 weeks, body weight gains of OB-BIO and OB-DF groups were lower by 3.3 and 4.8%, respectively as compared with that of OB-CON group. Although not significant, measured values of the perirenal and visceral fat pads of OB-BIO and OB-DF groups were lower than those of the OB-CON group. The weight of interscapular brown adipose tissue did not show significant difference in all groups. The size of adipocyte in rats was lower in both OB-BIO and OB-DF groups. Thus, biocelluose and the diet formulation showed the anti-obesity effect.

• The Korean Journal of Physiology and Pharmacology
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• 제21권6호
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• pp.633-641
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• 2017

The aim of this study is to investigate the effects of intermittent ladder-climbing exercise training on mitochondrial biogenesis and ER stress of the cardiac muscle in high fat diet-induced obese middle-aged rats. We induced obesity over 6 weeks of period in 40 male Sprague-Dawley rats around 50 weeks old, and were randomly divided into four experimental groups: chow, HFD, exercise+HFD, and exercise+chow. The exercising groups underwent high-intensity intermittent training using a ladder-climbing and weight exercise 3 days/week for a total of 8 weeks. High-fat diet and concurrent exercise resulted in no significant reduction in body weight but caused a significant reduction in visceral fat weight (p<0.05). Expression of $PPAR{\delta}$ increased in the exercise groups and was significantly increased in the high-fat diet+exercise group (p<0.05). Among the ER stress-related proteins, the expression levels of p-PERK and CHOP, related to cardiac muscle damage, were significantly higher in the cardiac muscle of the high-fat diet group (p<0.05), and were significantly reduced by intermittent ladder-climbing exercise training (p<0.05). Specifically, this reduction was greater when the rats underwent exercise after switching back to the chow diet with a reduced caloric intake. Collectively, these results suggest that the combination of intermittent ladder-climbing exercise training and a reduced caloric intake can decrease the levels of ER stress-related proteins that contribute to cardiac muscle damage in obesity and aging. However, additional validation is required to understand the effects of these changes on mitochondrial biogenesis during exercise.

• 대한한방내과학회지
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• 제37권6호
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• pp.1012-1021
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• 2016

This study investigated the effects of Wolbiga-banha-tang (WBBT) on high fat diet-induced rats. We divided the rats into 4 groups: normal (fed a general-fat diet), control (fed a high-fat diet and no treatment), $WBBT{\times}1$ (fed a high-fat diet and 250 mg/kg of Wolbiga-banha-tang extracts), and $WBBT{\times}2$ (fed a high-fat diet and 500 mg/kg of Wolbiga-banha-tang extracts). We measured the body weight, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, free fatty acids, total lipids, AST, ALT, and CBC in the serum of the obese rats for 7 weeks. There was a significant decrease in the control group, $WBBT{\times}1$, and $WBBT{\times}2$ in triglycerides and free fatty acids. The results suggest that WBBT is effective for lowering triglycerides and free fatty acids. Therefore, WBBT may be applied to the treatment of lipid metabolism in obesity and further clinical studies are needed.