• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.120-126
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• 2004

Kenaf plants were hydroponically grown in reactor containing toxic metals as Cd, Cu, Cr, Ni and Zn to examine the ability to take up heavy metal. The plants were fertilized using a nutrient solution, which was appropriately adjusted to optimum pH, DO and conductivity. For n hydraulic retention time of 8 days, Cr, Cd, Cu, Ni and Zn were removed up to 90.5, 80.5, 66.1%, 71.1% and 79.4%, and reduced from 2.34 to 0.54 mg $L^{-1}$, 3.37 to 1.07 mg $L^{-1}$, 4.92 to 3.19 mg $L^{-1}$, 6.31 to 4.41 mg $L^{-1}$ and 6.27 to 2.09 mg $L^{-1}$. Especially, accumulation rate of Cr, Cd, Cu, Ni and Zn in the plant were measured up to 347.32, 275.39, 157.52, 50.48 and 211.01 mg DW kg $L^{-1}d^{-1}$, respectively. We considered that Kenaf plants removed Cr, Cd and Zn more effectively than other toxic metals applied.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.132-136
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• 2012

Kenaf (Hibiscus cannabinus) is an annual herbaceous plant of the family Malvacease that has been planted in tropical Africa and Asia region for more than 4000 years and use as source of fiber, energy and feed stock. In this study, the physiological characters and chemical compositions of kenaf mutant variety "Jangdae" developed using gamma irradiation at the Korea Atomic Energy Research Institute (KAERI) were compared with three genetic resources (Auxu, C12, and C14-DRS). Jangdae showed the highest productivity growth rates in fresh yield, dry weight (DW) yield (leaf and stem), node number, and stem thickness. Especially, leaf DW yield of Jangdae was 1.6-3.1 times higher than that of three genetic resources. Also, stem DW yield of Jangdae was 1.6-2.1 times higher than that of three genetic resources. In the analysis of chemical composition, Jangdae showed 16.9% of crude protein content that was 0.86-0.94 times lower than three cultivars. However, Jangdae showed the highest neutral detergent fiber (NDF) contents in leaf (32.5%) and stem (75.2%). Also, acid detergent fiber (ADF) contents of stem and leaf in Jangdae were 64.4% and 33.9%, respectively. Total polyphenol and total flavonoid contents were 22.1 mg/g and 7.4 mg/g in Jangdae. Based on these results, Jangdae would have the potential to become a successful forage crop.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.375-384
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• 2014

Kenaf (Hibiscus cannabinus L.) was recognized as a potential source of forage. But the domestic cultivation techniques are not set standards yet. So we tested the basic culture techniques during 2012~2013 for getting a high yield and good grade forage production system. The best seeding method for mechanized planting (corn planter used) was hill seeding with $20{\times}20cm$ seeding distance. When we treated hill seeding with $20{\times}20cm$, the yield what we could get was 13,641 kg/10a and it was 32% more than that of conventional practice hill seeding with $20{\times}30cm$ seeding distance. The proper seeding date for getting high yield was May 1. In May 1, the yield per 10a was reached 13,423 kg, and it was 30% more than that of seeding at May 30. More over the crude protein content which was important factor for determinating forage nutritive value was 12.7% and it was higher 1.8% (relatively 16.5% high) than that of May 30 seeding. The most effective herbicide for kenaf was Fluazipof-p-butyl. It's herbicidal rate was 97% and phyto-toxicity was less than 5%. Regional adaptability for Jeollabuk-Do including Imsil Gun, Kochang Gun and Sunchang Gun were identified that the stable cultivation were possible in these area with average yield 12,400 kg/10a and it was about 1.7 times as compared to corn harvest.

• Natural Product Sciences
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• v.23 no.3
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• pp.192-200
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• 2017

Skin aging is a complex biological process due to intrinsic and extrinsic factors. Free radical oxidative is one of extrinsic factors that induce activation of collagenase, elastase and hyaluronidase. Natural product from plants has been used as antioxidant and antiaging. This study aimed to evaluate antioxidant and antiaging properties of Hibiscus sabdariffa extract (HSE) and its compounds including myricetin, ascorbic acid, and ${\beta}$ carotene. The phytochemical of H. sabdariffa was determined using modified Farnsworth method and presence of phenols, flavonoids and tannins were in moderate content, whereas triterpenoids and alkaloids were in low content. Total phenolic content performed using Folin-Ciocalteu method, was $23.85{\mu}gGAE/mg$. Quantitative analysis of myricetin, ${\beta}-carotene$, and ascorbic acid of HSE was performed with Ultra-High Performance Liquid Chromatography (UHPLC) that shows $78.23{\mu}g/mg$ myricetin, $0.034{\mu}g/mg$ ${\beta}-carotene$, whilst ascorbic acid was not detected. HSE has lower activity on DPPH ($IC_{50}=195.73{\mu}g/mL$) compared to ${\beta}-carotene$, the lowest in ABTS assay ($IC_{50}=74.58{\mu}g/mL$) and low activity in FRAP assay ($46.24{\mu}MFe(II)/{\mu}g\;$) compared to myricetin, ${\beta}-carotene$. Antiaging was measured through inhibitory activity of collagenase, elastase, and hyaluronidase. HSE had weakest collagenase inhibitory activity ($IC_{50}=750.33{\mu}g/mL$), elastase inhibitory activity ($103.83{\mu}g/mL$), hyaluronidase inhibitory activity ($IC_{50}=619.43{\mu}g/mL$) compared to myricetin, ${\beta}-carotene$, and ascorbic acid. HSE contain higher myricetin compared to ${\beta}-carotene$. HSE has moderate antioxidants and lowest antiaging activities. Myricetin is the most active both antioxidant and antiaging activities.

• Journal of Life Science
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• v.26 no.12
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• pp.1415-1421
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• 2016

This study was worked to investigate the antioxidant activity and the blood circulation improvement effect of two Hibiscus syriacus extracts divided into fermented H. syriacus extract and non-fermented H. syriacus extract. As the results of in vitro experiment, both of fermented H. syriacus extract and non-fermented H. syriacus extract had the DPPH free radical scavenging effect and the high contents of polyphenol and flavonoid known as antioxidant components in dose-dependent manner. Six weeks female C57BL/6 mice were divide into four groups and fed high diets for 28 days. Also fermented H. syriacus extract and non-fermented H. syriacus extract was daily intraperitoneal administration for 28 days at 2 mg/kg of body weight of mice. In vivo C57BL/6 mice experiment of normal diet group (NOR), high-fat diet group (CON), high-fat diet plus fermented H. syriacus extract group (FHS) and high-fat diet plus non-fermented H. syriacus extract group (NFHS), each of total cholesterol, triglyceride and low density lipoprotein (LDL) was decreased in both of FHS and NFHS groups, meanwhile high density lipoprotein (HDL) was increased (p<0.01). Conclusion, the results confirmed that H. syriacus containing the high contents of the antioxidant activity components had the significant effects of blood circulation improvement.

• Korean Journal of Agricultural and Forest Meteorology
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• v.9 no.1
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• pp.55-60
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• 2007

This study was conducted to find characteristics of photosynthesis for dwarf and street tree cultivars of Hibiscus syriacus L. Characteristics of growth and flowering on some cultivars were investigated. The photosynthetic capacity for the cultivars was also measured. Bulsae showed the best height increase; Soyang represented the lowest growth. Baektanshim ${\times}$ Kyungbuk1 had the largest number of branches and leaves. Sundeok and Bulsae were the best in terms of height and width of flower, Soyang showed the smallest flowers. According to the analysis of chlorophyll content (chlorophyll a, b, total) for the cultivars, there was little difference between Sundeok and Bulsae. Based on light response curves for cultivars, it was found that Baektanshim ${\times}$ Kyungbuk1 and Soyang had lower light compensation and light saturation points than Bulsae and Sundeok. Cultivars Baektanshim ${\times}$ Kyungbuk1 and Soyang were also low in dark respiration, photosynthetic capacity, and net apparent quantum yield. In conclusion, it is recommended that cultivars Baektanshim ${\times}$ Kyungbuk1 and Soyang might be used as a dwarf type due to photosynthetic capacity.

• Research in Plant Disease
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• v.8 no.1
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• pp.55-58
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• 2002

In July 2001, rotting and shivering flowers of cotton rose (Hibiscus mutabitis) were fecund in the flower beds along the roadsides in Jinju area. The disease first started as water-soaking, dark-green lesions on the petals, and then whole flower was rotted rapidly, Whitish mycelia and monosporous sporangiophore with monosporous sporangiola were formed abundantly on the lesions. Colony appeared as white to pale yellowish brown mycelia on potato dextrose agar medium (PDA). Monosporous sporangiophore was long slender and branched at the apex, each branch bearing a head of sporangiospores. Sporangium was subglobose in shape and was 42.6-114.2$\mu$m in size. Monosporous sporangiola were elliptic, fusiform or ovoid, and brown in color and 12.3~21.6 $\times$8.3~11.6$\mu$m Um in size. Sporangiospores were elliptic, fusiform or ovoid in shape, dark brown or brown in color and 16.3~23.8$\times$8.2~13.6$\mu$m in size, and they had three or more appendages at bipolar end. Zygospores were mostly globose, dark black colored and sized was 46.2-78.4$\mu$min diameter, The fungus grew on PDA between at 15 to 4$0^{\circ}C$, and the optimum temperature was 3$0^{\circ}C$. This is the first report on the flower rot of cotton rose caused by C. cucurbitarum in Korea.

• Korean Journal of Organic Agriculture
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• v.11 no.4
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• pp.103-115
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• 2003

In order to investigate the possibility for use to control environmental pollution, Kenaf(Hibiscus cannabinus L.) was used to obtain information of their growth and $CO_2$ response under different temperatures and $CO_2$ concentration. The highest percentage of germination and aboveground dry mass of Kenaf were found at 30$^{\circ}C$ and 35$^{\circ}C$ by 89.0% and 3.2g, respectively under different temperatures. The amount of $CO_2$ absorption and aboveground dry mass production of Kenaf were higher than those of maize during the whole growing period and the last sampling of aboveground dry mass of Kenaf and maize were 252.9g and 200.8g, respectively. The highest plant height was found at 400ppm by 131.0cm and the next was in the order of 600ppm by 1293cm, and 800ppm by 108.8cm. Leaf area was higher in the order of 400ppm > 600ppm > 800ppm, whereas leaf dry mass was in the order of 800ppm > 600ppm > 400ppm under different $CO_2$ concentration, showing that leaf became thicker as $CO_2$ concentration was increased. Days from seeding to flowering became shorter by 13 days in 35/25$^{\circ}C$ compared with 25/15$^{\circ}C$ between two temperature regimes and they also became shorter as $CO_2$ concentration was increased. Aboveground dry mass was higher in 35/25$^{\circ}C$ than that of 25/15$^{\circ}C$ between two temperature regimes. while it was increased in the order of 800ppm > 600ppm > 400ppm as CO2 concentration was increased. Temporal changes of leaf dry mass during growth period showed no difference between $CO_2$ concentration in 25/15$^{\circ}C$ , but the highest of it was found at 800ppm in 35/25$^{\circ}C$. The highest temporaI increase of root dry mass was found at 800ppm in 25/15$^{\circ}C$, but 35/25$^{\circ}C$ showed no difference between different $CO_2$ concentration.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.150-160
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• 2006

This study was performed to provide basic information of the development and breeding of new cultivars of Hibiscus syriacus L., which have more beautiful and diverse flowers. Morphological characteristics of the leaves and genetic variation of the leaves of two Jeoktanshim-line cultivars, Bulsae and Pyungsung, were crossed each other. The result of the cross between Bulsae and Pyungsung are as follows: Mean leaf length and width were 6.09cm and 3.36cm, respectively. Mean petiole length was 1.08cm, and mean shoulder width and incision depth were 1.50cm and 0.84cm, respectively. Mean leaf base angle was $98.56^{\circ}$. Leaf index and petiole length were the most important components of leaf characteristics. Two groups were clustered in distance level 5.4. The result of the cross between Pyungsung and Bulsae are as follows: Mean leaf length and width were 5.25cm and 3.10cm, respectively. Mean petiole length was 0.87cm, and mean shoulder width and incision depth were 1.50cm and 0.76cm, respectively. Mean leaf base angle was $107.87^{\circ}$, which was the widest among ten cross combinations. Two groups were clustered with seven leaf characteristics in distance level 4.2. Group I included two small groups, and group II included four small groups.

• Journal of Korean Society of Forest Science
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• v.85 no.4
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• pp.577-585
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• 1996

To seek effective methods for evaluating air pollution and acid rain injury, artificial acid mist(pH 2.5, 3.5 and 4.5) and ground water(pH 6.5) were treated on the potted seedlings of Ligustrum obtusifolium, Cercis chinensis, Hibiscus syriacus and Sophora japonica. Leaf chlorophyll contents, characteristics of leaf-injury, wettability-measurement of diameter of water-droplets on the leaf surface-among treatments were investigated. The results were summarized as follows. 1. Chlorophyll contents of Ligustrum obtusifolium and Hibiscus syriacus measured on June 3 were highest in pH 2.5 plot, but those of Cercis chinensis and Sophora japonica were relatively low level. Chlorophyll contents of Ligustrum obtusifolium measured on August 24 was highest in pH 2.5 plot, but those of Cercis chinensis, Hibiscus syriacus and Sophora japonica were highest in the control. 2. Changes of chlorophyll contents with acid mist treatments were differed among tree species. 3. For all the tested species, leaf injury(injured leaf number and rate, and injured leaf area) increased with decreasing pH levels of acid mist. 4. Leaf tissue injury seemed to be related with the wettability of the leaf surface. Measurement of diameter of water-droplets on the leaf surface might be useful criteria for acid rain or acid mist injury for the glabrous leaved species, such as, Cercis chinensis, Sophora japonica, etc.