• Journal of Ecology and Environment
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• 제43권1호
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• pp.1-8
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• 2019

Background: Hibernating bats exhibit ubiquitous winter activity in temperate zones, but there is considerable between- and within-species variety in their intensity and purpose. Bats may fly during winter for sustenance or travel to other hibernacula. This study compared inter-regional variation in the winter activity of the greater horseshoe bat (Rhinolophus ferrumequinum). We predicted that weather and hibernacula-environmental conditions would influence winter activity patterns. Results: Winter activity patterns differed between regions. In the Anseong area, we confirmed movement inside the hibernaculum, but in Hampyeong, we observed movement both inside and between hibernacula. The two regions differ by $4^{\circ}C$ in average winter temperatures. Anseong experiences 22 days during which average daily temperatures exceeded $5^{\circ}C$, whereas Hampyeong experienced 50 such days. During the hibernating period, bat body weight decreased by approximately 17-20% in both regions. Conclusions: Ambient temperatures and winter-roost environments appear to be behind regional differences in hibernating bat activity. As winter temperatures in Korea do not favor insect activity, feeding probability is low for bats. However, bats may need to access water. At Anseong, underground water flows inside the hibernaculum when the reservoir outside is frozen. At Hampyeong, the hibernaculum does not contain a water source, but the reservoir outside does not freeze during winter. In conclusion, water-source location is the most likely explanation for regional variation in the winter activity of hibernating bats.

• Journal of Ecology and Environment
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• 제32권3호
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• pp.191-195
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• 2009

We monitored 38 hibernation sites of Myotis formosus in South Korea and recorded the number of bats occupying each site and assessed the micro-climate at the sites during four winters from 2005 to 2009 at. The mean rock temperature of the bat roosting sites was $13.2{\pm}1.4^{\circ}C$ and the mean body temperature of the hibernating bats was $13.3{\pm}1.3^{\circ}C$. The number of hibernating bats was negatively related to the size of the entrance and positively related to the minimum ambient temperature and humidity in the site interior. More bats hibernated in roosts with smaller entrances and higher minimum ambient temperatures, and more bats selected sites presenting a narrow temperature range. This study showed that the internal environments of hibernacula of M. formosus were highly stable despite dramatic variation in the external environment.

• Applied Microscopy
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• 제16권2호
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• pp.61-74
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• 1986

The anatomy of the renal papilla and pelvis of the bat(Pipistrellus abramus abramus Temminck) and their ultrastructure of the epithelium has been studied by means of light, scanning and transmission electron microscopy. The bats were captured at Ondal cave located in Yangchun-Myun, Tanyang-Kun, Chungchongpuk-Do, Korea in the active(July) and hibernating(January) phases. A conical renal papilla with several papillary foramina at the apex was curved toward the ureter. At the margin of the funnel-shaped renal pelvis, the fornix extended deeply into the kidney parenchyme was formed between the outer zone of the medulla and the cortex. The epithelium of the papilla covered the inner zone of the medulla. The epithelium of the fornix medially overlayed the outer zone of the medulla, and laterally the cortex. The surface epithelium of the papilla was simple columnar in shape with the scanty organelles and the short microvilli on their luminal surface. The fornix was Jined with $1{\sim}2$ cell layered squamous epithelium. On the medial surface of the fornix, two types of the superficial epithelial cells were identified; one had numerous short microvilli and the other had the microplicae and plaques on their luminal surface. The lateral surface of the fornix lined with the epithelial cells showed microridges and plaques on the luminal surface. The epithelial cells on the medial and lateral surfaces of the fornix were similar to their internal ultrastructures. The pelvic epithelium was a typical transitional type, which was composed of a layer of basal cells, one or two layers of intermediate cells, and a layer of superficial(facet) cells. The fusiform vesicles were observed in the cytoplasm of the intermediate and facet cells. There were no noticeable morphological changes in the epithelium of the papilla, fornix and pelvis except the medial epithelium of the fornix in the hibernating cycle. The microvillar cells were more numerous in the hibernating phase than in the active phase. The morphology, of the renal papilla and fornix of bats, indicates that a functional relationship may exist between the pelvic urine and the renal parenchyme.

• 생태와환경
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• 제51권4호
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• pp.345-353
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• 2018

온대지역에 분포하는 박쥐가 생존을 위하여 선택하는 동면은 에너지가 고갈되는 시기에 직면하는 에너지 문제해결을 위한 적응현상이다. 본 연구에서 온대지역에 분포하는 토끼박쥐의 온도선호도와 동면전략(동면기간)에 대한 연구를 수행하였다. 박쥐의 온도선호도와 동면전략과의 연관성을 알아보기 위하여 박쥐의 온도선호도는 동면기간에 영향을 준다는 가설 검증을 하였다. 이를 위하여 토끼박쥐의 분포를 확인하였고 동면처의 환경특성 및 토끼박쥐의 온도선호도를 도출하였다. 또한 토끼박쥐는 외부 최저 기온이 온도선호도보다 낮아지는 시기에 동면처에 도착하여 외부 최저 기온이 온도선호도보다 높아지는 시기에 동면처를 떠날 것으로 예측하였다. 동면중인 토끼박쥐의 평균 체온은 $3.03{\pm}1.30^{\circ}C$(range $0.1{\sim}7.6^{\circ}C$, n=179)로 대기온도 ($T_a$)에 비해 암벽온도 ($T_r$)와 밀접하게 상관되었다. 토끼박쥐는 외부 기온이 온도선호도보다 낮아지는 11월 중순 이후에 동면처에 도착하여 115~120일 동안 동면처에 머물렀다. 또한 외부 기온이 온도선호도보다 높아지는 시기인 3월 중순 이후에 동면처를 떠났다. 본 연구의 결과는 토끼박쥐의 동면기간은 대상 종의 온도선호도와 외부기온과의 상호작용에 의한 것임을 시사하며 또한 대상 종의 온도선호도 및 동면기간 같은 동면전략은 대상 종의 분포 제한 요인으로 작용될 수 있다.

• 한국동물학회지
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• 제20권2호
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• pp.67-76
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• 1977

The periodic changes of testis and \ulcorner션 epididymidis in Korean hibernating bats, the oriental discoloured bats (Vesportilio superans Thomas) and the Korean greater horseshoe bats (Rhinolophus ferrumequinum korak Kuroda) were studied in order to clarify the possibility of correlation between their histological findings and one of physiological facets, hibernation, and the results obtained were as follows: 1. The spermatogenic function of the oriental discoloured bats obtained in July and August was depressed although the spermatocytes showed a considerable cell proliferation. Few mature sperms were observed in the seminiferous tubules of the bat obtained in August. 2. The spermatogenic function of the Korean greater horeshoe bats obtained in September was not remalkable but a considerable number of sperms were stored in the excretory ducts which were characterized by existence of para-tubular spaces in the ductus epididymidis. 3. The spermatogenic epithelia of the Korean greater horseshoe bats obtained in December showed histologically atrophied figures. However, a vast number of sperm remained in extremely expaned luminae of the ductuc epididymidis which epithelial cells were maintained rectangular in shape. 4. These results suggest that there are periodic changes of the spermatogenic epithelia and the excretory ducts, and that those histological changes are closely related to their wintering.

• 생태와환경
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• 제50권4호
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• pp.411-421
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• 2017

온대지역에서 동면박쥐에 대한 분포와 생태정보는 대상 종의 보전 및 서식지 보호 관리의 효율성을 높인다. 본 연구에서 멸종위기종인 붉은박쥐 개체군 경향 분석과 생태적 요구 조건을 파악하여 보호 관리 방안을 제시하고자 하였다. 2007년부터 2016년까지 조사를 수행한 결과, 전국 58개의 동면처에서 붉은박쥐 570개체가 확인되었다. 붉은박쥐가 이용한 동면처의 유형은 자연동굴에 비해 폐광산이 많았으며, 폐광산에서 보다 더 많은 붉은박쥐 개체가 동면하는 것이 확인되었다. 붉은박쥐의 동면처는 평균 $12.13{\sim}15.07^{\circ}C$의 온도와 92% 이상의 습도가 동면기간 동안 안정되게 유지되었다. 장기적인 모니터링 자료를 바탕으로 멸종위기종인 붉은박쥐의 개체군의 변화를 평가하기 위하여 TRIM을 이용하여 분석한 결과, 붉은박쥐 개체군의 변화는 안정적인 것으로 평가되었다. 그럼에도 불구하고 일부 동면처 사례를 통하여 동면처의 구조 및 환경 변화로 인한 붉은박쥐 개체수가 급감되는 사실을 확인하였다. 본 연구는 장기 모니터링 결과에 근거한 개체군 경향분석 결과와 현장 사례를 분석을 통하여 멸종위기종인 붉은박쥐의 보전 및 서식지 보호 관리 방향을 제시한다. 따라서 멸종위기종인 붉은박쥐의 서식지 보호 관리 방안은 방향은 종의 온도 선호도 및 서식지 환경이 변화되지 않도록 유지하는 것과 서식지 내 인위적인 간섭요인을 차단하는 것은 중요 사항이 될 것이다.

• Applied Microscopy
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• 제22권2호
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• pp.97-117
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• 1992

In order to study process of spermiogenesis of the Korean greater horseshoe bat, Rhinolophus ferrumequinum korai, the cycle of seminiferous epithelium was examined by the light and electron microscope and the following results were obtained based on the epithelial cell differentiation. 1. Spermiogenesis occurred from early July to mid-Octber, and spermatogenic activity was vigorous from mid-August to late September. Spermatocytes including spermatogonia were found to be degenerated in only July. It is deduced that the degeneration serves as the mechanism to regulate effective use of energy to prepare for mating and hibernating periods, and regulation of breeding cycle. 2. Spermiogenesis of the Korean greater horseshoe bat was divided according to differentiation of the cell structure, into Golgi, cap, acrosome, maturation and spermiation phases; Golgi, cap and spermiation phases were further divided into two steps of early and late phase respectively, and acrosome phase into three steps of early, mid and late phases, and maturation phase has only one step. Hence, the spermiogenesis consists of ten phases. The first research was done in this article on the changes of chromatin with nucleus, the time of appearance and disappearance of chromatin granules, in case of Korean greater horseshoe bat (Rhinolophus ferrumequinum korai). Chromatin granule began to be condensed in late Golgi and the condensation proceeded to form an irregular mass of a electron-dense chromatin in a form of circular cylinder in the center of nucleus at the phase of maturation. Finally, the chromatin condensation proceeded and perfect nucleus of sperm with homogeneous density was formed when the sperm was separated from Sertoli cell. Therefore, appearance and disappearance of chromatin granules occurred in the period of time between late Golgi and maturation phase, The tail of sperm began to develop in early cap phase, Numerous lipid droplets were obseved in the cytoplasm of spermatids during the maturation phase, which seemed to be used as energy source necessary to make mature sperm during spermiogenesis.

• Applied Microscopy
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• 제30권1호
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• pp.21-44
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• 2000

동면기 (11월부터 3월까지) 동안의 한국산 관박쥐(Rhinolophus ferrumequinum korai) 자성 생식도관 내의 정자저장, 정자 생존 여부 및 정자이동을 알아보기 위하여 전자현미경으로 관찰한 결과는 다음과 같았다. (1) 자궁내강, 자궁선내의 정자들은 다수의 백혈구들에 의해 포식되고 소멸되었다. (2)정자들은 수란관 미측 협부에서만 저장되었고, 정자들의 두부는 상피세포쪽을 향하고 있었다. 이는 교미기(10월 초-중순경)에 사출된 정자들이 긴 동면기 동안에 수란관 미측 협부에서만 생존 가능함을 의미하며, 수란관의 미측 협부가 정자의 수정능 획득(capacitation)에 필요한 최적의 장소임을 의미한다. (3) 동면후기인 3월의 수란관 미측협부에는 정자들이 관찰되지 않았다. 이는 배란기인 4월에 난자를 만나기 위해 정자들이 수정부위인 팽대부 쪽으로 이행하였음을 의미한다. 이상의 결과로 미루어 보아, 긴 동면기 동안의 자성 생식도관 내에서의 정자의 장기저장과 생존여부 및 정자이동은 수정을 위한 일종의 mechanism이라 여겨진다.

• Applied Microscopy
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• 제15권2호
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• pp.10-18
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• 1985

The posterior hypothalamus of the hibernating greater horseshoe bats (Rhinolophus ferrumequinum korai Kuroda) were observed with an electron microscope. The posterior hypothalamus is known to be closely related to the reflex responses activated by cold, and the following observations were obtained in the cellular type of nerve cells: there are three types of neurons in the posterior hypothalamus. 1. The first type of neuron was the largest, ovoid or conical in shape, the nucleus was elliptic and the nuclear envelope had many deep invaginations. The cell organelles were well developed, in particular there was an abundance of variously shaped mitochondria, and the Golgi complex and the polysomes were observed in the cytoplasm. 2. The second type of neuron was moderate in size, ovoid or elliptic in shape, the nucleus was located nearer to the plasma membrane and the nuclear envelope had. a few invaginations. The cytoplasm was rich in amount compared with that of the third type of neuron, and the cell organelles, especially the rough endoplasmic reticulum were well developed. Also lipofuscin pigments were observed. 3. The third type of neuron was the smallest in size and round in shape. The nucleus and the nucleolus were observed in the central portion of the cell body and the nuclear envelope had a few invaginations. The cytoplasm was small compared with those of the first and second types, but the rough endoplasmic reticulum, the mitechondria and the polysomes were relatively well developed. The cytoplasm was characterized by the presence of membrane-bound small bodies with a single membrane containing a fine particular substance around the rough endoplasmic reticulum and the Golgi complexes.

• 한국동물학회지
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• 제39권1호
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• pp.98-105
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• 1996

온도 적응에 관한 연구들을 보면 생리기능 상의 최적온도가 생물 개체의 일상 체온에 잘 일치한다는 내용을 보여주고 있다. 동면 중의 박쥐들은 월동장소의 주위온도(9$^{\circ}$-12$^{\circ}$C)보다 약간 높은 범위에서 체온을 유지하는 것으로 관찰되고 있다. 본 연구에서는 이 동물들의 근육이 동면체온 범위에서 최대의 수축기능을 발휘하도록 조정되리라는 가설을 설정하고 이를 실험하기 위해 한국큰과박쥐 Rhinolophus ferrumequlnum korai (n = 5)의 이두박근 수축기능을 $10^{\circ}$-35$^{\circ}$C범위의 근육온도에서 실험하였다. 상대적 강축힘(최대 강축힘에 대한 퍼세트 값)은 이들의 동ㅁㄴ체온과 일치하는 $10^{\circ}$-15$^{\circ}$C 범위에서 가장 높았다. 한편 비동면성 동물은 37$^{\circ}$C의 범주에서 체온을 유지하며 강축힘에 대해 30$^{\circ}$-40$^{\circ}$C 범위의 최적온도를 보이는데 이를 종합해 볼 때 본 연구의 결과는 박쥐의 비행근 기능이 계절적인 체온변화에 준하여 조정되고 있음을 보여준다고 하겠다. 박쥐의 근육이 낮은 체온에서도 강한힘을 발생한다는 본 실험 결과는 동면동안 이따금 수분섭취나 배설을 위해 날아야하는 점 또는 천적으로부터 도피할 수 있어야 하는 점을 고려할 때 적응의 한 예라고 사료된다.