• Analytical Science and Technology
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• v.20 no.3
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• pp.219-226
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• 2007

Glass is frequently encountered as types of materials that are submitted to forensic science laboratories as a result of trace evidence transfers. The repeatability and the reproducibility of trace element analysis were presented. An analysis of variance (ANOVA) was performed on laser ablation inductively coupled plasma spectrometric analyses of the fragments to identify the source. Pairwise comparisons were completed for all samples. In a pairwise comparison, each sample was compared to each other for a possible [n(n-1)/2] (n : numbers of the samples) total comparison to associate/discriminate samples using Tukey's HSD method. The aim of this study was to determine the utility of LA-ICP-MS for multi-element analysis of forensic samples. The 12 glass fragments from two manufacturers were collected and analyzed to identify the source. An analysis of variance (ANOVA) was performed on 31 elements in NIST 612 Trace elements in Glass. Elements were classified into four categories defined by the combination of precision and variation of inter-samples. We selected 11 elements, 209Bi, 90Zr, 121Sb, 178Hf, 59Co, 238U, 208Pb, 140Ce, 118Sn, 49Ti and 137Ba. 6 pairs out of 66 possible pairs were not distinguished when compared by 137Ba (p<0.05). However, all samples were distinguished using both 49Ti and 137Ba (p<0.05). In conclusion, multi-elemental analysis with LA-ICP-MS is a potential tecnique for the discrimination of forensic samples.

• Journal of Korean Biological Nursing Science
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• v.16 no.4
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• pp.334-341
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• 2014

Purpose: The purpose of this study was to examine the effects of aroma-necklace application on perceived stress, symptoms of stress and changes in autonomic nervous system among nursing students in clinical training. Methods: A research design for this study was a non-equivalent control group, non-synchronized pre-post test. The experimental group (n=39) underwent aroma inhalation by necklace which contains an essential oil mixture of lavender and sweet orange mixture in ratio of 2:1 for 2 weeks while the control group (n=36) received placebo therapy. The data were collected using self-administered questionnaires and measurement of heart rate variability (HRV) and analyzed using the PASW 18.0 program. Results: The perceived stress scores and scores of peripheral manifestations in the experimental group were significantly lower than those of the control group. All indices of HRV were significantly different between the two groups. LF norm and LF/HF ratio in the experimental group were significantly lower than those of the control group, and HF norm in the experimental group was significantly higher than that in the control group. Conclusion: It can be suggested that aroma-necklace application was effective in decreasing perceived stress and peripheral manifestations of stress and in changing HRV indices among nursing students in clinical training.

• Mass Spectrometry Letters
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• v.3 no.2
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• pp.58-61
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• 2012

A method based on flow injection-isotope dilution-cold vapor-inductively coupled plasma mass spectrometry (FI-IDCV-ICP/MS) has been applied to determine trace level of mercury in fly ash. $^{200}Hg$ isotopic spike was added to 0.25 g of BCR176R fly ash and then decomposed by microwave digestion procedure with acid mixture A (8 mL $HNO_3$ + 2 mL HCl + 2 mL HF) and acid mixture B (8 mL $HNO_3$ + 2 mL $HClO_4$ + 2 mL HF) for applying IDMS. Mercury cold vapor was generated by using reductant solution of 0.2% (w/w) $NaBH_4$ and 0.05% (w/w) NaOH. The measurements of n($^{200}Hg$)/n($^{202}Hg$) isotope ratio was made using a quadrupole ICP/MS system. The accuracy in this method was verified by the analysis of certified reference material (CRM) of fly ash (BCR 176R). The indicative value of Hg in BCR 176R fly ash was $1.60{\pm}0.23$ mg/kg (k = 2). The determined values of Hg in BCR 176R fly ash by the method of FI-CV-ID-ICP/MS described in this paper were $1.60{\pm}0.24$ mg/kg (k = 3.18) and the analysis results were in well agreement with the indicative value within the range of uncertainty.

• The Journal of Korean Medicine
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• v.33 no.1
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• pp.68-78
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• 2012

Objectives: The purpose of this study was to analyze the relationship between Yin-deficiency questionnaire score and various biofunctional signals in women. Methods: A retrospective chart review was performed on charts of 195 patients who visited Gangdong Kyung Hee Hospital between April 1st and September 30th, 2011. The subjects were categorized into two groups, a low Yin-deficiency group (n=118) and a high Yin-deficiency group (n=77). The authors analyzed the correlation between Yin-deficiency questionnaire score and biofunctional signals by Pearson's correlation coefficient test and the difference in biofunctional signals between the two groups by independent samples t-test using SPSS for windows. Results: 1. Negative correlations were observed between the temperature difference of back-humerus, standard deviation of all R-R intervals (SDNN), total power (TP), low frequency (LF), high frequency (HF) on heart rate variability parameters, and Yin-deficiency questionnaire score. A positive correlation was observed between the temperature difference of knee-humerus and Yin-deficiency questionnaire score. 2. The temperature difference of back-humerus in the high Yin-deficiency group was significantly higher than that in the low Yin-deficiency group. The temperature difference of knee-humerus, height, waist-hip ratio, SDNN, TP, LF, and HF of the high Yin-deficiency group were significantly lower than those of the low Yin-deficiency group. Conclusions: The results of this study suggest that the comprehensive diagnosis of Yin-deficiency and biofunctional signals is useful.

• Journal of Oriental Neuropsychiatry
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• v.20 no.4
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• pp.127-135
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• 2009

Objectives : This study was conducted to assess the effect of Chunwangbosim-dan(CB) on the autonomic nervous system by using heart rate variability measurement. Methods : The eligible subjects were composed of the healthy female group of ages 25 to 30 years. They were divided into two groups, the CB group(n=16) and the control group(n=16). We measured the heart rate variability respectively for 3 times at 14:00, 16:00, and 17:00. In the CB group, subjects were administered CB at 15:00. For the measurement values, Mean Heart Rate and SDNN(standard deviation of the NN intervals) were used as time domain analysis, and HF(high frequency), LF(low frequency), and LF / HF ratio were used as frequency domain analysis. Results : The degree of LF changes in the CB group was significantly lower than control group at 1 hour after administration. Conclusions : We suggest that Chunwangbosim-dan may be useful for stabilization of the autonomic nervous system by controlling sympathetic nerve in healthy people.

• Journal of Korean Medicine Rehabilitation
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• v.19 no.2
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• pp.241-250
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• 2009

Objectives : To study autonomic nervous system dysfunction of Low Back Pain(LBP) patients, using spectral analysis of Heart Rate Variability(HRV). Methods : HRV of 190 patients was measured and seperated into two groups, those with LBP(n=95) and healthy controls(n=95). HRV was measured by SA-6000(Medicore, Korea) for 5 minutes after 5 minutes' resting. Results : 1. Mean heart rate(MHRT) of the experimental group was slightly higher than that of the control group, but did not show significant difference(P=0.428). The square root of the mean squared differences of successive normal-to-normal intervals(RMSSD), logarithmic very low frequency power(Ln VLF) and low frequency power/high frequency power ratio(LH/HF ratio) were not significantly low between experimental group and control group(P=0.16, 0.130, 0.537). 2. The standard deviation of all the normal-to-normal intervals(SDNN), logarithmic total power(Ln TP), logarithmic low frequency power(Ln LF) and logarithmic high frequency power(Ln HF) were significantly low between experimental group and control group(P=0.03, 0.005, 0.001, 0.007). 3. Ln LF of acute group was significantly low compared with those of chronic group(P= 0.039). Conclusions : This study suggests the activity and imbalance of autonomic nervous system in LBP is low. Also sympathetic nervous system of acute LBP is lower than that of chronic LBP. Further study of HRV related to LBP is needed in the clinical medicine.

• Journal of Digital Contents Society
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• v.8 no.3
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• pp.355-363
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• 2007

One of major factors for learning achievement is the student's learning preference according to his character type. In course of learning, if a student studies e-learning contents opposed to his preference, then he would be under stress and his blood pressure and heart beat be changed. For measuring unwillingness, we used spectral components in frequency domain known as stress measure. For 13 children attending kindergarten we examined S(sensing)/ N(intuition) of MBTI and presented same learning contents during 10 minutes. During learning we gathered ECG signals, changed into HRV(heart rate variability), transformed time-varying HRV signal into spectral density in frequency domain. And then, we divided it into three areas of low(LF), middle(MF), and high-frequency(HF) and calculated stress measures by rates of those frequency area. We compared estimated stress measures of S group with them of N group whether students in different group preferred different contents or not. Experimental shows that students according to MBTI type prefer different contents.

• Journal of the Korean Chemical Society
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• v.34 no.3
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• pp.232-238
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• 1990

To study the biological roles of Zn, we investigated simple model systems of $Zn^{++}, coordinated with OH_2 or NH_3,$ or with O=C- in peptide. The geometrical structures and net atomic charges were calculated by the ab initio HF-SCF theory using double zeta basis sets. The ligands of O-H, N-H, and O=C- are very polar due to $Zn^{++}$. Therefore, the carbon atom in peptide becomes so electrophilic that it can be easily attacked by other nucleophiles. In addition, to understand how $Zn^{++}$ is coordinated with ligands in enzyme, a molecular mechanics method is applied to the system of the enzyme of carboxypeptidase A (CPA) with the substrate of glycyltyrosine. From our results, it appears that the Zn ion is coordinated not only by four ligands in enzyme and substrate but also by one water molecule.

• Korean Journal of Biological Psychiatry
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• v.16 no.2
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• pp.69-75
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• 2009

Objectives : The detrended fluctuation analysis is one of the nonlinear methods for the investigation of biological time series. It quantifies the fractal scaling properties and is known to be useful in the evaluation of long-range correlations in time series. The heart rate variability(HRV) of obstructive sleep apnea syndrome (OSAS) patients during nighttime was analyzed by detrended fluctuation analysis to assess its relationship with the severity of the symptoms. Methods : Fifty nine untreated male OSAS patients with moderate to severe symptoms(mean age=45.4${\pm}$11.7 years, apnea-hypopnea index, AHI${\geq}$15) underwent nocturnal polysomnography. Moderate(AHI=15-30, N=22) and severe(AHI>30, N=37) OSAS patients were compared for the indices derived from detrended fluctuation analysis and frequency domain analysis of HRV. Results : In the detrended fluctuation analysis, the alpha values were 0.75${\pm}$0.11 and 0.82${\pm}$0.07 for the severe and the moderate OSAS groups respectively. The difference was significant(p<.01). The alpha value had negative correlation with AHI(r=-.425, p=.001). Negative correlation coefficients were also found in the relationships between the alpha values and very low frequency(VLF)(r=-.425, p=.001), low frequency(LF)(r=-.633, p= <.001) and the LF/HF ratio(r=-.305, p=.019) respectively. LF/HF ratio(p=.005) was higher in the severe OSAS group compared to that of the moderate OSAS group. Conclusion : In this study, the detrended fluctuation analysis showed the significant difference between the two OSAS groups classified according to their severity of symptoms. The scaling exponent showed the negative correlation with AHI and indicies of frequency domain analysis. This result suggests that detrended fluctuation analysis can be helpful to estimate the severity of OSAS.

• Journal of Sasang Constitutional Medicine
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• v.28 no.2
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• pp.123-131
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• 2016

Objectives We observed what effects of Paced Breathing(PB) in specific respiration rate have on heart rate variability (HRV) according to Sasang Constitution.Methods HRV of 72 healthy participants in sitting position was recorded while they carried out usual breathing, 0.2Hz, 0.1Hz, and 0.05Hz PB each 5 minutes in consecutive order. Five minute of relaxation was permitted between each breathing. Finally, HRV indices were statictically analyzed of 32 participants (SOEUM: 11, SOYANG: 10, TAEEUM: 11) after data out of accord with respiration rate or outliers were excluded.Results and Conclusions According to respiration rates, there was no statistical significance of HRV among Sasang Constitution. Regardless of Sasang Constituion, 0.2Hz PB increased mean heart rate and decreased natural logarithmic low frequency(lnLF) oscillation of HRV without the change of natural logarithmic high frequency(lnHF), while 0.1Hz PB increased lnLF and standard deviation of N-N interval(SDNN), and slightly decreased lnHF without the change of mean heart rate. 0.05 Hz PB also showed the same effect as 0.1Hz PB, but was impracticable.