• Title/Summary/Keyword: Hetero-chitosan

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Preparation of Hetero-Chitooligosaccharides and Their Antimicrobial Activity on Vibrio parahaemolyticus

  • Park, Pyo-Jam;Lee, Hun-Ku;Kim, Se-Kwon
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.41-47
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    • 2004
  • This study was performed to investigate the antimicrobial effects of hetero-chitosans and their oligosaccharides on the halophilic bacterium, Vibrio parahaemolyticus. Nine classes of hetero-chitosan oligosaccharides were prepared based on their molecular weights, using an ultrafiltration membrane reactor system with chitosanase and celluase, from partially different deacetylated chitosans, 90%, 75%, and 50% deacetylated chitosan, respectively. Thirty-two strains of V. parahaemolyticus were isolated from various marine organisms such as shellfish, shrimps, octopus, and seabirds. Seventy-five percent deacetylated chitosan showed the highest antimicrobial acitivity. The minimal inhibitory concentration (MIC) was 0.5 mg/ml on 14 strains of V. parahaemolyticus, and MIC of the rest strains (18 strains) was 1.0 mg/ml. In addition, MIC of most hetero-chitosan oligosaccharides was 8.0 mg/ml. The results revealed that the antimicrobial effects of hetero-chitosans and their oligosaccharides against V. parahaemolyticus depend on the degree of deacetylation, their molecular weights, and strains tested.

Antimicrobial Activity of Hetero-Chitosans and Their Oligosaccharides with Different Molecular Weights

  • Park, Pyo-Jam;Je, Jae-Young;Byun, Hee-Guk;Moon, Sung-Hoon;Kim, Se-Kwon
    • Journal of Microbiology and Biotechnology
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    • v.14 no.2
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    • pp.317-323
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    • 2004
  • This study was performed to investigate the antimicrobial effects of hetero-chitosans and their oligosaccharides against three Gram-negative bacteria and five Gram-positive bacteria. Nine classes of hetero-chitosan oligosaccharides consisted of partially deacetylated chitosans; 90%, 75%, and 50% deacetylated chitosans. Based on molecular weight, they were prepared using an ultrafiltration membrane reactor system. Seventy-five percent deacetylated chitosan showed the highest antimicrobial acitivity as compared with the 90% and 50% deacetylated chitosan, and the activity was dependent on their molecular weights. It was apparent that the growth of Gram-negative bacteria is less inhibited in the presence of the heterochitosans and their oligosaccharides than Gram-positive bacteria. These results revealed that the antimicrobial effects of hetero-chitosans and their oligosaccharides depend on the degree of deacetylation, and their molecular weights.

Chitosan surface grafted with fusion protein of FGF-2 and Fibronectin-FGF for tissue regeneration therapy

  • Hwang, Jeong-Hyo;Lee, Jue-Yeon;Kim, Sun-Chul;Jang, Jun-Hyeog;Ku , Young;Chung, Chong-Pyoung;Lee, Seung-Jin
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.231.3-232
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    • 2003
  • The biomedical applications of chitosan have been widely researched. FN mediates its biological effects through binding to the hetero-dimeric transmembrane glycoproteins, integrins, which physically couple the cytoskeleton to the ECM. FN binds to the integrin through a consensus site including the Arg-Gly-Asp (RGD) sequence within tenth type III module (Ruoslahti & Pierschbacher 1987). A short sequence Pro-His-Ser-Arg-Asn (PHSRN) has also been identified as a synergistic motif within ninth type III module for binding to ${\alpha}$5${\beta}$1 integrin (Aota et al. 1994). (omitted)

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