• Journal of Korean Clinical Health Science
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• v.1 no.1
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• pp.83-89
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• 2013

Purpose. Natural herb has been used for traditional medicine for thousands of years. For this reason, alternative medicine methods affect to domestic animal field. The main purpose of this study was to confirm the regulated proteins after feed additive treatment. Methods. We used total thirty male pigs were used for this experiment. E. coli and Salmonella typhimurium, were used for promote enteritis. Animals were divided into a negative control group, positive control group and test group to determine the effect of an additive mixture on the changes in protein expression in serum. The pigs were given a food supplemented with a natural herbal additive containing immunoglobulin yolksac (IgY) at concentrations 1%. On the 1st week and after eight weeks of feeding, the serums were collected from each group. The changes in the serum proteome as a response to the herbal additive were examined using two-dimensional polyacrylamide gel electrophoresis. Results. A total of 14 differentially expressed protein spots were identified by comparing the protein profiles of the control and additive treated porcine serums. Finally, 7 proteins were detected by MALDI-TOF/MS. Moreover, the proteins detected are involved in a cholesterol control factor, inflammation regulator, Conclusion. These results support of the hypothesis that a natural herbal additive containing IgY can affect the immune regulation system and reduce the pathological process by microbial infections.

• Journal of the Korean Dietetic Association
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• v.8 no.2
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• pp.199-205
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• 2002

In this study, we found a new food additive as an natural herbal extracts against lipid digestion enzymes for the regulation of fatty acid absorption and weight control. The Water extracts of Platycodon grandiflorum and Solanum melongena. inhibited lipase activity and decresed serum total cholesterol and triglyceride concentration in mouse fed lipid emulsion. Twenty three volunteers were subjected to the intake of the herbal extracts plus the egg yolk IgY that inhibit carbohydrate digestion enzymes in gut for 50 days. In average, the treated subjects appeared to lose 1.96 kg of body weight and 3.4 kg of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of natural herbal extract and egg yolk IgY improve the obesity by the decrement of body weight and body fat mass.

• Journal of Veterinary Clinics
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• v.27 no.1
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• pp.17-22
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• 2010

The natural herbal antimicrobial additive, Flavo-$SK^{TM}$, was developed by Zoonosis Research Center of Wonkwang University. The purpose of this study was to evaluate the effects of Flavo-$SK^{TM}$ on the health status and performance of growing chickens. This study was conducted on the growing chickens (n=20,000) for 31 days in a growing chickens husbandry. The animals were divided with two groups; Flavo-$SK^{TM}$ treated group (n=10,000) and commercial diet feeding group (n=10,000). The Flavo-$SK^{TM}$ treated animals had provided with commercial diet adding the Flavo-$SK^{TM}$ as 0.29%. During the study period, we compared clinical signs, weight increase rate, diet consumption amount, gross finding, necropsy findings and histopathological findings between the treated group and non treated group. As the results of this clinical trial, the natural herbal antimicrobial additive, Flavo-$SK^{TM}$, showed the effects on disease reduction. It is suggested that Flavo-$SK^{TM}$ has the antimicrobial effects.

• Korean journal of food and cookery science
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• v.18 no.3
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• pp.319-324
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• 2002

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymer for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment if blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum Melongena, Twenty three volunteers were subjected to the intake of the egg yolk IgY Plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 kg of body weight and 3.4 kg of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

• Journal of Mushroom
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• v.13 no.3
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• pp.266-269
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• 2015

This study was carried out to investigated optimum additive ratio of barley flour for substitution of rice bran at cultivation of winter mushrooms. Mycerial growth was faster according to increase of barley flour ratio than those of controls, but only some slow at the addition of 10% barley flour. Yields of fruiting body show the highest to 165.4 g/850 ml of medium which are the addition 30% of barley flour and not significant difference of yields up to the addition of 70% barley flour. Diameter of pileus was the highest at the addition of 30% barley flour. Hardness of pileus and stipes were the highest at the addition of 10% barley flour. The L value of stipes were the hight at the addition of barley flour, but the L value of pileus were decreased at the addition of barley flour, but there was no significant difference in the a-value and the b-value.

• Journal of the East Asian Society of Dietary Life
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• v.12 no.3
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• pp.249-257
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• 2002

This study was conducted to investigate the effects of medicinal plants with additives on storage characteristics of sausage. The water activity of the sausage with the additives tended to decrease with the storage time: the highest value was shown in the Angelica added sausage samples, in both cases added with dried powder as well as with extracted powder. The Paeonia extract powder added sausage had the lowest pH value. The herbal plant added sausages showed the lowest cooking and storage losses among all. The values of TBA (thiobarbituric acid value) and VBN (volatile basic nitrogen) increased with storage time, but the values of the sample with the extract powder additive were lower than those of the freeze-dry powder additive. Total plate counts of bacteria increased with storage time fur all samples, but those for the samples with Pueraria, Platycodon, Angelica, Bupleurum and Paeonia additives exhibited lower values than the control. From the above results, the sausages with medicinal plant additives showed the superior storage safety and quality characteristics.

• Herbal Formula Science
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• v.25 no.3
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• pp.391-412
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• 2017

Objective : In this study, we searched the experimental research about combined treatment of anticancer drug and herbal medicine for killing or inhibiting proliferation of Cancer cells searched in OASIS and KISS. This study aimed to analyze the experimental research paper about anticancer drug combined treatment with herbal medicine. Methods : We collected the research paper including killing or inhibiting proliferation of Cancer cells in OASIS and KISS using keyword anticancer drug with herbal medicine, tumor suppressor with herbal medicine, inhibition of Cancer with herbal medicine and combined treatment with herbal medicine. Assorting by cancer cells, we analyzed experimental results cancer cell viability, anticancer drug dosage, tumor weight and survival rate. Also, we checked the effects of herbal medicine on cancer and additive effect reducing side effect of anticancer drug. Results : Total 45 studies were selected. 38 studies reported combined treatment of anticancer drug and herbal medicine was more effective than only anticancer drug. The death of cancer cells was synergistically induced by the cotreatment of anticancer drug and herb extracts. The studies suggest that the cotreatment of anticancer drug and herb extracts could reduce side effect of anticancer drug. In addition, some studies reported cotreatment mechanism like apoptotic death signal processes. In combined treatment of anticancer drug and herb extracts, The expression of Fas/Fas L, Bax, Bcl2, Caspase-3 etc.. was markedly increased in cancer cells. Conclusions : Our results suggest that anticancer drug combined treatment with herbal medicine could be efficient for killing or inhibiting proliferation of cancer cells. However, this paper had some limitation as follows: First, collected studies have been published only for korean journal. Second, results of research and effects of combined treatment are not collected objectively. To solve these problems, more objective and balanced studies should be performed.

• The Journal of Korean Medicine
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• v.24 no.4
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• pp.64-70
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• 2003

Background and Purpose : Allergic rhinitis is a well-known, relatively controllable chronic disease. Although a number of methods for treating allergic rhinitis have been tried, many patients have not been satisfied with their treatment. Therefore, this study tried to evaluate the effect of a cooperative system of Oriental and Western medicine and to develop a new diagnosis protocol for treatment of allergic rhinitis. Methods : We measured improvement rate and acoustic rhinometry after the allergeninduction test and performed a filter paper test as a nonspecific hypersensitivity test with 60 patients who are allergic to house dust mite. Patients were divided into two groups, one treated with Western medicine only and one treated with both Western and Oriental herbal medicine. For the group with Western medicine only, antihistamine for one week and local steroid medicine for two weeks were prescribed. For the group with combined medicine, Oriental herbal medicine was prescribed according to the patient s constitution, along with Western medicine. After all treatments, the above tests were re-performed and the improvement rate was compared. Results and Conclusion : We observed better results in the group treated with both Western and Oriental herbal medicines, comparing improvement rate and the alteration of total nasal volume through acoustic rhinometry after the allergen induction test. In the filter paper test, there was no significant difference between the two groups. In conclusion, we showed the additive effect of Oriental herbal medicine without any severe side effects compared with treatment with Western medicine only. In this study, we set only two patient groups, but further study is required to create various experimental groups and compare among them. We suggest that it might enhance understanding of the improved effect of Oriental herbal medicine in the therapy of allergic rhinitis.

• Mycobiology
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• v.49 no.6
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• pp.589-598
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• 2021

White strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analysis, 82 backcrossed strains (HM8 lines) were generated by mating between KMCC03106-31 and the progenies of the F1 hybrid (Hami-18 × KMCC03106-93). Using HM8, the first 23 quantitative trait loci (QTLs) of yield-related traits were detected with high limit of detection (LOD) scores (1.98-9.86). The length, thickness, and hardness of the stipe were colocated on LG 1. Especially, length of stipe and thickness of stipe were highly correlated given that the correlation coefficients were negative (-0.39, p value ≤ .01). And a typical biomodal distribution was observed for lightness of the pileus and the lightness of the pileus trait belonged to the LG 8, as did traits of earliness and mycelial growth in potato dextrose agar (PDA) medium. Therefore, results for color traits can be suggested that color is controlled by a multi-gene of one locus. The yield trait was highly negatively correlated with the traits for thickness of the stipe (-0.45, p value ≤ .01). Based on additive effects, the white strain was confirmed as recessive; however, traits of mycelial growth, lightness, and quality were inherited by backcrossed HM8 lines. This new genetic map, finely mapped QTLs, and the strong selection markers could be used in molecular breeding of H. marmoreus.

• Journal of Animal Science and Technology
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• v.49 no.3
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• pp.321-328
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• 2007

Two pig trials were conducted to test Herb MixⓇ, a mixture of Rehmannia glutinosa, Angelica gigas, Discorea japonica, Glycyrrhiza uralensis, Schisandra chinensis and Ligusticum jeholense, as a herbal additive to weaning pig diet. Exp. 1 was conducted with 45 three-way cross-bred(Y×L×D) weaning pigs randomly allocated to 3 treatments; control, Herb MixⓇ 0.15% and Herb MixⓇ Gold(Plellidendron amurense fortified Herb MixⓇ) 0.15%. Exp. 2 was conducted with 48 weaning pigs randomly allocated to 4 treatments; control, 0.1%, 0.2% and 0.3% Herb MixⓇ. There was a significant(p=0.05) difference between the control and herbal additive groups, however, no significant difference was found between Herb MixⓇ and Herb MixⓇ Gold in growth performance of Exp. 1. In Ex. 2, supplementation of Herb MixⓇ at all level(0.1%, 0.2% and 0.3%) significantly(P<0.05) improved average daily gain and feed intake, however, there were no significant differences among supplemented groups. Among the blood parameters, serum IgG level and WBC numbers were significantly lowered by Herb Mix supplementation in both experiments. Stress indicator(SI) was significantly lower in herbal additive groups in Exp. 1. Nutrient digestibility of DM and NFE in supplemented groups was lower than the control in Exp. 1. Howener, it was not significantly different among treatments in Exp. 2. Number(cfu) of fecal E.coli decreased while that of Lactobacilli increased in treated groups. It was concluded that fortifying Herb MixⓇ with Plellidendron amurense was not effective in improving the efficacy of Herb MixⓇ and supplementation of Herb MixⓇ at 0.1~0.2% level improves growth performance of weaning pigs. Blood parameters especially immunity related ones(IgG, WBC and SI) were significantly influenced.