• 대한한의학방제학회지
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• 제8권1호
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• pp.343-358
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• 2000

Epimedii Herba is an important traditional herb medicine widely used as a tonic. However, the mechanism of action of this drug on the immune system is not well studied. This study was performed to elucidate the effects of the aqueous extract of Epimedii Herba on the production of antibodies in mice. Antibodies in serum were detected in male ICR mice treated with the aqueous extract of Eplmedii Herba (AEEH) at a dosage of 40, 120, and 360mg/kg orally for 2 weeks. Effects of oral administration of AEEH on antibody forming responses were measured by enzyme linked immunosorbent assay (ELISA) of immunoglobulin (Ig) levels in serum. The results were as follows; 1. Epimedii Herba slightly decreased body weight gain. 2. Epimedii Herba significantly increased the relative spleen weight. 3. Epimedii Herba significantly increased total serum IgG levels. 4. Epimedii Herba significantly enhanced total serum IgG1 levels. 5. Epimedii Herba significantly increased total serum IgG2a levels. 6. Epimedii Herba markedly increased total serum IgM levels. These findings demonstrate that Epimedii Herba significantly enhances the production of antibodies at therapeutic concentrations, and suggest that administration of Epimedii Herba may prevent the host from immunologic diseases.

• 대한약침학회지
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• 제14권4호
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• pp.13-22
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• 2011

Objectives: The symptoms of hypothyroidism are fatigue, cold intolerance, arthralgia, muscle cramps, dry skin and etc. Although hypothyroidism is a relatively common endocrinical disease, we do have many difficulties treating it effectively. The symptoms of hypothyroidism are similar to those of Yang-deficiency in Oriental medicine. Epimedii Herba is a popular herb that has the effect of tonifying the kidney and strengthening Yang in Oriental medicine. In this study, we investigated the therapeutic effects of Epimedii Herba on a hypothyroidism rat model induced by PTU(6-Propyl, 2-thiouracil). Methods: 24 two-month-old Spargue-Dawley(SD) rats were divided into 4 groups: 1) normal(n=6), 2) PTU-induced hypothyroidism control(n=6), 3) hypothyroidism rat treated with Epimedii Herba(n=6), 4) hypothyroidism rat treated with levothyroxine(n=6). PTU was administered for 4 weeks, Epimedii Herba and levothyroxine was administered for 2 weeks after PTU was initiated for a total duration of 2 week. At the end of the experiment, blood samples from all the rats were taken from their hearts and were analyzed. Results: In comparison with normal group, the PTU-induced control group significantly showed hypothyroidism with low T3, T4 and high TSH. In Epimedii Herba group, T4 was significantly increased(p<0.05). There was no significant difference in TSH between the Epimedii Herba treatment group and the control group. And no significant differences were observed in biochemical labs and weight between the Epimedii Herba group and the control group. Conclusions: These results suggest that Epimedii Herba could help thyroid cells to produce thyroid hormones. And no significant side effects related with Epimedii Herba were found, suggesting that it is safe to administer. According to these results, Epimedii Herba may be a safe alternative medicine for hypothyroidism.

• 대한한방부인과학회지
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• 제18권1호
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• pp.142-155
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• 2005

Objective: This study was undertaken to evaluate the dose dependent effects of Epimedii Herba extract solution on the spermatogenic abilities such as concentration, motility and morphological normality of sperm from the testis, and the activities of spermhyaluronidase and antioxidants. Materials and Method: We choose the 2-month-old mice, and administered the extract powder of Epimedii Herba in the different concentration once in a day for 60 days. The control group was administerde to normal water in the isolated testis tissue. Also we observed changes of isolated testis at the before and after administration of Epimedii Herba extracts in the mice. And we compared to the testicular tissue especially seminiferous tubules between control and treated group by histochemical methods. Results: The significant dose dependent differences were observed in the concentration of total sperm, the motility and normality of spermatozoa of the Epimedii Herba extract administered groups compared than that of control group, respectively. In the histological analysis of the testicular lobes were observed in the Epimedii Herbaextract administered groups than control group, respectively. Also, the activity hyluronidase was significantly increased in the Epimedii Herba extract administered groups than that of the control group. In case of antioxidant activity analysis, the activity of peroxidase and catalase were significantly increased in the Epimedii Herba extract administered groups than that of control group, respectively. Conclusion: This study shows that Epimedii Herba can effect the count and motility of sperm, the important ractor in male fertility and also promote the activity of antioxidants, catalase and peroxidase, which is the important factor in spermatogenesis.

• 생약학회지
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• 제32권1호통권124호
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• pp.43-48
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• 2001

Epimedii Herba has been used for tonic, cardiotonic, impotency, amnestic and diuretic. Icariin, a main component was isolated from the Epimedii Herba and identified by the spectroscopic methods. In order to evaluate the quality of Epimedii Herba purchased from various regions of Korea, quantitative determination of icariin in Epimedii Herba using HPLC method has been conducted. Quantitative analysis showed the average amount of icariin in commercial 42 samples was 0.34% on the dry weight basis.

• Biomolecules & Therapeutics
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• 제20권2호
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• pp.189-195
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• 2012

Epimedii Herba is a traditional medicinal herb used in Korea and China and exerts estrogenic activity. In this study, we investigated the effect of peripubertal administration of Epimedii Herba on pubertal development in female rats using a modified protocol of the rodent 20-day pubertal female assay. Female Sprague-Dawley rats (21 days old after weaning, 10 rats per group) were divided into five groups: saline (Con), ethinyl estradiol (E2), Epimedii Herba ext (Ext), icariin (ICI), and icaritin (ICT), which were administered by oral gavage (E2 by subcutaneous injection) from postnatal day (PND) 21 through PND40. The time to vaginal opening (VO) was shorter for the Epimedii groups, particularly for the ICT group (p<0.05). Treatment with ICI and ICT significantly increased the duration of the estrus cycle (ICI, 2.78 days; ICT, 4.0 days; control, 1.78 days). Ovary weight was reduced by E2 treatment and increased by the Ext, ICI, and ICT treatments while the weight of the uterus and pituitary glands increased significantly only in the E2 and ICT groups. Although Epimedii Herba displayed relatively weak estrogenic activity, its repeated administration could affect pubertal development in female rats.

• 대한본초학회지
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• 제28권6호
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• pp.111-117
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• 2013

Objectives : This research aimed at studying the immuno modulating activity of Fermented Epimedii Herba (EHS). Method : The impacts on the cell viability, hydrogen peroxide and nitric oxide (NO) generation in cells, and cytokines such as tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin (IL)-6, IL-$1{\beta}$, monocyte chemoattractant protein-1 (MCP-1) level have been measured by using Raw 264.7 cells with the specimen EHS as the fermented extract of Epimedii Herba with Saccharomyces cerevisiae STV89. Result : As a result of MTT assay to confirm the cytotoxicity of extracts from fermented Epimedii Herba, the toxicity was not excessively induced in Raw 264.7 cells when EHS were processed by concentration. EHS increased hydrogen peroxide generation in Raw 264.7 cells. EHS suppressed NO generation in Raw 264.7 cells while they significantly suppressed the increase of NO generation induced by LPS in macrophage. EHS significantly decreased the generation amount of TNF-${\alpha}$ and IL-6 induced by LPS in Raw 264.7 cells at $25{\mu}g/mL$ or more. Conclusion : It appeared that the fermented extract of Epimedii Herba manufactured from Epimedii Herba significantly has the immuno modulating acitivity as it did not excessively trigger cytotoxicity to Raw 264.7 cells, increased hydrogen peroxide generation in Raw 264.7 cells, decreased NO generation in macrophage, and especially, suppressed both TNF-${\alpha}$ and IL-6 generation in macrophage induced by LPS.

• 혜화의학회지
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• 제9권1호
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• pp.215-223
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• 2000

To understand immunopharmacologic effects on Cervi Pantotrichum Cornu, Morindae Officinalis Radix, Cistanches Herba, Curculginis Rhizoma, Epimedii Herba, Eucommiae Cortex, we investigated chinese experimental documents, and we could reach conclusions as follows : 1. The effects on cell-mediated immune system were as follows. 1) The effects on macrophage (1) The herbal medicines promoting to increase the number of WBC in the peripheral blood were Morindae Officinalis Radix, Epimedii Herba and that promoting to reinforce the phagocytic functions of neutrophil was Curculginis Rhizoma. (2) The herbal medicines promoting the phagocytic functions of mononuclear, macrophage were Cervi Pantotrichum Cornu, Cistanches Herba, Eucommiae Cortex. 2) The herbal medicines stimulating the activities of T lymphocytes were Cervi Pantotrichum Cornu, Curculginis Rhizoma, Epimedii Herba, Eucommiae Cortex. 2. The effects on humoral immune system were as follows. 1) The herbal medicines increasing the activity of complement receptor were Cervi Pantotrichum Cornu, Curculginis Rhizoma. 2) The herbal medicines reinforcing immunity of spleen cells were Cervi Pantotrichum Cornu, Cistanches Herba, Epimedii Herba. 3) The herbal medicines promoting proliferation of spleen cells that produce antibody after having been immunized by SRBC were Cervi Pantotrichum Cornu, Cistanches Herba, Epimedii Herba. 3. The herbal medicines, reinforcing immunity on delayed type hypersensitivity were Cervi Pantotrichum Cornu, Cistanches Herba, Eucommiae Cortex. As you know in the many bibliological documents, the studies on the effects of Drugs for Tonifying Yang were started along right lines. Recently the studies on those were accomplished more rapidly and applied many immune diseases. We thought that Drugs for Tonifying Yang could be important immunopotentiators. Therefore we can apply those herbal medicines not only to immune diseases but also inflammatory diseases, senile infirmity and all sorts of tumor.

• KSBB Journal
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• 제17권4호
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• pp.388-395
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• 2002

장내 유용세균인 Eifidobacterium adolescentis KCTC 3216은 천연산물 향가지 단독으로서도 성장이 촉진되었으며, 두가지, 세가지 및 네가지의 조합에 의하여도 특징적으로 성장이 촉진되었다. 그리고 이 균주는 천연산물의 배합 중에서 한가지의 천연산물로서는 괴화, 황백, 상백피, 진피, 당귀 등이 좋은 효과를 보였고, 두가지 천연산물의 배합에서는 백작약와 녹차, 음양곽과 당귀, 백작약과 음양곽, 백출과 당귀 등의 조합이, 세가지의 천연산물 배합에서는 녹차와 백작약 및 음양곽, 녹차와사간 및 백작약, 녹차와 황기 및 상백피 등의 조합이, 그리고 4가지 천연산물 배합에서는 사간, 당귀, 음양곽 및 녹차의 조합, 당귀, 음양곽, 백작약 및 녹차의 조합, 음양곽, 백작약, 괴화 및 녹차 등의 조합에서 잘 성장함을 보였으며, 음앙곽, 백작약, 괴화 및 녹차의 조합이 대조군 보다 2.6배의 성장 촉진 효과를 나타내었고, AUC는 659.88로써 대조군의 AUC보다 5.6배 높은 것으로 나타났다. 또한 4종류의 조합 중 당귀, 음양곽, 백작약 및 녹차의 조합의 AUC가 665.55로써 강한 것으로 났으며, 이 조합의 성장촉진효과는 대조군보다 2.1배의 성장률을 보였고 대조군의 AUC(116.61)보다 5.7배 정도 항산화력이 강한 것으로 나타났다 또한 4가지 종류 조합의 총 phenol 량은 녹차 중의 증제차나 볶음차 둥의 비발효 차보다 30배 이상 높은 것으로 나타났으며, 반 발효차인 오룡차보다는 32∼35배, 발효차인 홍차보다는 55∼60 정도 높은 것으로 나타났다. 이것은 항산화력이 강한 천연산물들을 이용하여 free radical의 제거와 동시에 B. adulescentis의 성장 촉진 물질로 사용할 수 있으며, 생체의 기능을 향상시킬 수 있는 기능성 제제로의 응용 가능성을 보였다.

• 약학회지
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• 제40권2호
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• pp.230-237
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• 1996

The fractions of Epimedii Herba were examined for the immunological effects in ICR mice. Mice were divided into 4 groups and administered orally the fractions of Epimedii Herba for 10 days. The results of this study were summarized as following: (1) The fraction 1 (EtOAc layer) administered group as compared with control group significantly decreased spleen weight, Arthus reaction and hemagglutination (HA) titer but significantly increased circulating white blood cells (WBC). (2) The fraction 2 ($H_20$ layer) administered group as compared with control group significantly decreased liver weight, Arthus reaction and HA titer but significantly increased WBC. (3) The fraction 3 (ppt) administered group as compared with control group significantly increased liver weight, thymus weight rate, delayed type hypersensitivity, phagocytic activity and WBC. The results showed that Frs. 1 and 2 administered groups reduced humoral immune response but increased WBC, and that Fr. 3 administered group increased cell-mediated immune response, phagocytic activity and WBC.

• 한국식품영양과학회지
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• 제31권5호
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• pp.931-938
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• 2002

유용세균인 B. infantis은 선택되어진 천연산물의 한 종류 단독으로서도 촉진되었으며, 또한 두 종류, 세 종류, 네 종류의 배합에 의하여 특징적으로 성장이 촉진되었으며, 이것은 항산화력이 강한 천연산물들을 free radical의 제거와 동시에 B. infantis의 성장촉진물질로 사용할 수 있고 이를 이용하여 기능성 항산화 제제의 제조가 가능함을 의미하며 이들 천연산물의 배합 중에서 한 종류의 천연산물로서는 진피, 두충 ,산수유 그리고 황기 등이 좋은 효과를 보였고, 두 종류 천연산물의 배합에서는 음양곽과 녹차, 음양곽과 산사자, 음양곽과 두충 등의 조합이, 세 종류의 천연산물 배합에서는 녹차와 음양곽 및 백굴채, 녹차와 음양곽 및 진피, 녹차와 백굴채 및 황기, 녹차와 진피 및 갈근 등의 조합이, 4종류 천연산물 배합에서는 녹차, 황기 및 음양곽 그리고 갈근의 조합, 녹차, 황기 및 진피 그리고 갈근의 조합, 녹차, 백굴채 및 황기 그리고 음양곽 등의 조합에서 B. infantis가 잘 성장함을 보였고, 녹차, 백굴채 및 황기 그리고 음양곽의 조합이 대조군보다 2.1배 이상의 성장률을 보였다. 4종류의 조합의 항산화력을 용존산소 분석법에 의하여 분석한 결과 녹차, 백굴채 및 황기 그리고 음양곽의 조합이 632.49로써 가장 높았고, 다음으로 녹차, 음양곽 및 진피 그리고 갈근의 조합(623.64),녹차, 백굴채 및 진피 그리고 갈근의 조합(601.81), 녹차, 백굴채 및 황기 그리고 갈근의 조합(560.49)순서로 나타났으며, 항산화력이 강한 조합에서 성장이 촉진되는 것으로나타났다. B. infantis의 성장을 촉진하는 4종류의 조합에 대한 hydroxyl radical의 소거 활성은 50,100,200 및 300 배로 희석하여 검토하였을 때 녹차, 백굴채 및 진피 그리고 갈근의 조합을 100배로 희석한 소거윤이 84.36%로 가장 높은 소거율을 보였으며 ,4 종류의 조합모두 놀은 희석율에도 80% 이상의 높은 소거율을 보여 높은 희석률을 필요로 하는 음료 제제로서의 탁월함을 보였다. 또한, 4종류의 각 조합에 따른 방향족의 함량 및 총 Phenol 함량은 녹차 중의 증제차나 볶음차의 비 발효 차보다 28～30배 이상 높은 것으로 나타났으며, 반발효차인 오룡차보다는, 32～33배, 발효차인 홍차보다는 55～58배정도 높은 것으로 나타났다 이것은 항산화력이 강한 천연산물들을 이용하여 free radical의 제거와 동시에 B. infantis의 성장 촉진물질로 사용할 수 있으며 생체의 기능을 향상시킬 수 있는 기능성 제제로의 응용 가능성을 보였다.