Effects of the Aqueous Extract of Epimedii Herba on the Production of Antibodies in Mice

음양곽(淫羊藿) 수추출물(水抽出物)이 생쥐의 항체생성(抗體生成)에 미치는 영향(影響)

  • Kim Joung-Hoon (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park Joung-Suk (Dept. of Pharmacy, Graduate School of Wonkwang University) ;
  • Lee Kwan-Sun (Dept. of Oriental Medicine, Graduate School of Wonkwang University) ;
  • Lim Kyu-Sang (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Kwang-Gyu (Dept. of Pathology in Wooseok University) ;
  • Woo Won-Hong (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 김정훈 (원광대학교 한의학전문대학원) ;
  • 박정숙 (원광대학교 대학원 약학과) ;
  • 이관순 (원광대학교 대학원 한의학과) ;
  • 임규상 (원광대학교 한의학전문대학원) ;
  • 이광규 (우석대학교 한의과대학 병리학교실) ;
  • 우원홍 (원광대학교 한의학전문대학원)
  • Published : 2000.12.05

Abstract

Epimedii Herba is an important traditional herb medicine widely used as a tonic. However, the mechanism of action of this drug on the immune system is not well studied. This study was performed to elucidate the effects of the aqueous extract of Epimedii Herba on the production of antibodies in mice. Antibodies in serum were detected in male ICR mice treated with the aqueous extract of Eplmedii Herba (AEEH) at a dosage of 40, 120, and 360mg/kg orally for 2 weeks. Effects of oral administration of AEEH on antibody forming responses were measured by enzyme linked immunosorbent assay (ELISA) of immunoglobulin (Ig) levels in serum. The results were as follows; 1. Epimedii Herba slightly decreased body weight gain. 2. Epimedii Herba significantly increased the relative spleen weight. 3. Epimedii Herba significantly increased total serum IgG levels. 4. Epimedii Herba significantly enhanced total serum IgG1 levels. 5. Epimedii Herba significantly increased total serum IgG2a levels. 6. Epimedii Herba markedly increased total serum IgM levels. These findings demonstrate that Epimedii Herba significantly enhances the production of antibodies at therapeutic concentrations, and suggest that administration of Epimedii Herba may prevent the host from immunologic diseases.

Keywords

References

  1. 黃帝內徑素問譯解 楊維傑
  2. 大韓韓醫學會誌 v.10 no.1 免疫學에 관한 文獻的 考察 黃黃義玉
  3. 大韓韓醫學會誌 v.11 no.2 免役疾患의 韓方槪念과 治療에 관한 文獻的 考察 蔡禹錫
  4. 임상본초학 신민교
  5. Zhejiang J. Trad. Clin. Med. v.20 Clinical applications of Yinyanghuo Yang, Z.Z.
  6. Chinese Drug of Plant Origin Tandg, W.;Eisenbrand, G.
  7. Chin. J. Pharm. Anal. v.4 Analysis of active ingredients of traditional Chinese herbal drug;Assay of icariin in Epimedium Liu, C.R.;Xu, L.X.
  8. Oriental matrica medica Hong, H.
  9. J. Trad. Chin. Med. v.12910 Clinical observation on treatment of 120 cases of coronary heart disease with Herba Epimedii Yu, L.;Li, H.;Huang, G.;Bai, Y.;Dong, Y.
  10. Chin. Trad. Herb Drugs v.13 Effects of icariin on heart Liu, C.M.;Yu, Q.H.;Zhang, L.M.
  11. Chung Kuo Chung Yao Tsa Chih v.22 no.4 Preventive effect of gubao on hydrocortisone-induced osteoporosis in rats Xie, H.;Wu, T.;Huang, L.;Li, C.
  12. Chung Kuo Chung Yao Tsa Chih v.21 no.12 Experimental study on antagonizing action of Herba Epimedii on side effects induced by glucocorticoids Wu, T.;Cui, L.;Zhang, Z.;Chen, Z.;Li, Q.;Liao, J.;Huang, L.
  13. Chem. Pharm. Bull. v.37 no.7 Differential inhibitory effects of various herbal extracts on the activaties of reverse transcriptase and various deoxyribonucleic acid polymerases Ono, K.;Nakane, H.;Zeng-Mu, M.;Ose, Y.;Sakai, Y.;Mizuno, M.
  14. J. Immunol. v.109 Enzyme-linked immunosorbent assay III:Quantitation of specific antibodies of enzyme labeled anti-immunoglobulin in antigen-coated tubes Enavall, E.;Perlmann, P.
  15. 中醫 基礎學 上海中醫學院
  16. 中醫病因病機學 宋驚永
  17. 韓方病理學 總論 鄭遇悅
  18. 實用臨證中藥指南 林通國(編著)
  19. 上海中醫藥雜誌 v.6 虛證和實證病人的免疫狀態 章育正
  20. 東洋醫學 v.12 no.1 免疫에 關한 東洋醫學的 考察 趙鍾寬
  21. Agents Actions v.15 Immunomodulatory effects of Panax gingeng C.A. Meyer in the mouse Jie, Y.H.;Cammisuli, S.;Baggiolini, M.
  22. Chin. Trad. Herb Drugs v.13 Effect of ginsenosides from stems and leaves of ginseng on immunological functions Cui, J.C.;Liu, A.J.;Wang, B.X.
  23. Chin. Pharm. Bull. v.16 Effect of polysaccharide of ginseng root on immnuological function Wang, B.X.;Cui, J.C.;Liu, A.J.
  24. J. Shemyang Coll. Pharm. v.3 Effects of ginseng root polysaccharides cyclophosphosphamide-suppressed immunity Yuan, W.X.;Shang, X.H.;Jin, M.L.;Shang, K.
  25. The effect of ginseng on immune responses;Advances in Chinese Medicinal Material Research Wang, B.X.;Cui, J.C.;Liu, A.J.;Chang, H.M.(ed.);Yeung, H.W.(ed.);Tso, W.W.(ed.);Koo, A.(ed.)
  26. Org. Chem. Studis of the active princeples of Astragalus mongholicus Bung-I. Isjolation, characterization and biological effect of its polysaccharides Fang, S.D.;Chen, Y.;Xu, X.Y.;Ye, C.Q.;Zhai, S.K.;Shen, M.L.
  27. Chin. Trad. Herb Drugs v.11 Isolation and identification of icariin Liu, B.Q.;Ma, H.S.;Mon, P.
  28. Heterocycles v.26 Validity of oriental medicines Part 122. Epimedins A, B and C, flavonoid glycosides of Epimedium koreanum Herbs Oshima, Y.;Okamoto, M.;Hikino, H.
  29. Chem. Pharm. Bull. v.35 Studies on the glycosides of Epimedium grandiflorum-I Miyase, T.;Ueno, A.;Takizawa, N.;Kobayashi, H.;Oguchi, H.
  30. Chem. Pharm. Bull. v.35 Studies on the glycosides of Epimedium grandiflorum-II Miyase, T.;Ueno, A.;Takizawa, N.;Kobayashi, H.;Oguchi, H.
  31. Chem. Pharm. Bull. v.36 Studies on the glycosides of Epimedium grandiflorum-III Miyase, T.;Ueno, A.;Takizawa, N.;Kobayashi, H.;Oguchi, H.
  32. Phytochemistry v.26 Flavonol glycosides from Epimedium sagittatum Mizuno, N.;Hanioka, S.;Suzuki, N.;Iinuma, M.;Tanaka, T.;Liu, X.S.;Min, Z.D.
  33. Chem. Pharm. Bull. v.36 no.9 Flavonol glycosides in Epimedium species Mizuno, N.;Iinuma, M.;Tanaka, T.;Sakakibara, N.;Hanioka, S.;Liu, X.S.
  34. Yakugaku Zasshi v.95 no.27 Studies on the constituents of flavonoids of Epimedium grandiflorum-IV Tokuoka, Y.;Daigo, K.;Takemoto, T.
  35. Phytochemistry v.27 Flavonol glycosides from Epimedium sagittatum Mizuno, M.;Sakakibara, N.;Hanilka, S.;Iinuma, M.;Tanaka, T.;Liu, X.S.;Shi, D.W.
  36. Phytochemistry v.27 Three flavonol glycosides from Epimedium koreanum Ito, Y.;Hirayama, F.;Suto, K.;Sagara, K.;Toshida, T.
  37. Chem. Pharm. Bull. v.32 Inhibitors of angiotensin-converting enzyme in crude drugs-I Inokuchi, J.I.;Okabe, H.;Yamauch, T.;Nagamatsu, A.
  38. Chem. Pharm. Bull. v.33 Inhibitors of angiotensin-converting enzyme in crude drugs-II Inokuchi, J.I.;Okabe, H.;Yamauch, T.;Nagamatsu, A.;Nonaka, G.;Nishioka, I.
  39. Chung Hua Nei Ko Chih v.33 no.2 Effects of Epimedium sagittatum on immunopathology and extracellular matrices in rats with chronic renal insufficiency Chung, Q.L.;Chen, X.M.;Shi, S.Z.
  40. Chung Kuo Chung Yao Tsa Chih v.16 no.10 Effects of Epimedium sagittatum Maxim;Polysaccharides on DNA synthesis of bone marrow cells of "yang deficiency" animal model caused by hydroxyurea Liu, F.;Ding, G.;Li, J.
  41. Kor. J. Pharmacogn. v.20 Studies on the effect of several crude drugs on cultured chicken brain cells Park, M.J.;Song, J.H.;Kim, Y.C.
  42. Yakugaku Zasshi v.110 Phagocytic activity of leaves of Epimedium species on mouse reticuloendothelial system Iinuma, M.;Tanaka, T.;Sakakibara, N.;Mizuno, M.;Matsuda, H.;Shiomoto, H.;Kubo, M.
  43. U.S. Patent 4501734 Extract from barrenwort Matsushita, S.;Takahash, M.;Yoshida, S.;Weber, I.;Yamauchi, K.
  44. U.S. Patent 4528188 Polysaccharide PS-A obtained from barrenwort deriving from plats belonging to the Genus Epimedium, process for preparation and phylactic and immunostimulating agents comprising said polysaccharide PS-A effective component Matsushita, S.;Takahashi, M.;Yoshida, S.;Weber, I.;Takahashi, M.;Yoshida, S.;Weber, I.
  45. Abstr. Chin. Med. v.1 Advances on antiageing herbal medicines in China Chen, K.J.;Zhang, W.P.
  46. Shanghai J. Immunol. v.6 In vivo and in vitro studies on the effect of Epimedium extract on mouse immune response Wang, Y.C.;He, Q.Z.
  47. J. Trad. Chin. Med. v.26 Correction between trace elements and immunological function in patients with vital energy deficiency Liu, F.C.;Liu, J.X.;Ding, G.X.;Zhang, J.Y.;Zhou, S.H.;Guo, F.;Wu, Y.C.;Hu, T.H.
  48. Yakhak Hoeji v.40 no.2 Effects of Epimedii Herba fraction on immune response on ICR mice Kim, J.H.;Kim, I.H.;Chae, B.S.;Kang, T.W.;Park, C.B.;Ahn, Y.K.
  49. Immunology of cytokines: An introduction;The cytokine handbook Vicek, J.;Le, J.;Thomson, A.W.(ed.)
  50. J. Immunol. v.143 The role of interferon-gamma in delayed type hypersensitivity mediated by Th1 clones Fong, T.A.;Mosmann, T.R.
  51. Science v.236 Interferon-gamma and B cell stimulatory factor-1 reciprocally regulate immunoglobulin isotype production Snapper, C.M.;Paul, W.E.
  52. Annu. Rev. Immunol. v.7 Th1 and Th2 cells: Different patterns of lymphokine secretion lead to different functional properties Mosmann, T.R.;Coffman, R.L.
  53. J. Immunol. v.143 Evidence for differential induction of helper T cell subst during Trichinella spiralis infection Pond, L.;Wasson, D.L.;Hayes, C.
  54. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih v.15 no.4 Effect of Epimedium sagittatum on quality of life and cellular immunity in patients of hemodialysis maintenance Liao, H.J.;Chen, X.M.;Li, W.G.
  55. Chung Hua Nei Ko Tsa Chieh v.34 no.2 Effect of Epimedium sagittatum on soluble IL-2 receptor and IL-6 levels in patients undergoing hemodialysis Chen, X.;Zhou, M.;Wang, J.