• Herbal Formula Science
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• v.6 no.1
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• pp.141-156
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• 1998

This histological study was performed to investigate the effects of .Paljeungsan-gagambang on the epididymal fat cell size, number and the fat drop area of hepatic lobule of rats fed High Fat Diet. Paljeungsan-gagambang was orally administered to the rats of sample group 26mg per 200g of rat's weight everyday for 4 weeks, and the control group were just fed with equal amount of saline solution. Then they are the epididymal fat cell size, epididymal fat cell numberand fat drop area of hepatic lobule that were measured. The results were summarized as follows; 1. The size of the epididymal fat cell was decreased significantly as compared with the control group. 2. The number of the epididymal fat cells was decreased significantly as compared with the control group in distribution chart classified by cell size 3. The Area(%) of the Fat drops in the hepatic lobule was decreased significantly as compared with the control group. According to the above results, it is considered that Paljeungsan-gagambang have effects on the decrease of the epididymal fat cell size, number and fat drop area of hepatic lobule of rats fed high fat diet; Thus, the adminisrtation of Paljeungsan-gagambang is considered to have an improving effect on obesities and liver diseases caused by high fat diet.

• Applied Microscopy
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• v.25 no.4
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• pp.62-70
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• 1995

Rat liver sinusoids were observed by scanning electron microscopy. The sinusoids were provided with fenestrations which were divided into three types; small, medium-sized and large. The small fenestrations were usually gathered into clusters. The medium-sized fenestrations were either individually organized or in the cluster of small fenestrations. The large fenestrations were usually accompanied by small or medium-sized fenestrations. The lobule was divided into three zones; peripheral, intermediate and central, according to the distribution pattern of the fenestrations. The sinusoid providing with small fenestrations (type I) were predominant in the peripheral zone. The sinusoid showing medium-sized fenestrations (type II) were frequently observed in the central zone. The sinusoid having large fenestrations (type III) were abundant in the intermediate zone. This evidence indicate that the sinusoidal fenestrations become larger toward the intermediate zone from the peripheral zone of the lobule and progressively smaller toward the central zone. However, the fenestrations observed in the central zone seem to be larger than those seen in the peripheral zone of the lobule.

• Journal of Environmental Science International
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• v.6 no.1
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• pp.53-60
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• 1997

The toxicity of purified ricln from Ricinus communis to rats was examined by histological and histochemical methods. Sprague-Dawley rats were injected intraperitoneally with 75$\mu\textrm{g}$/kg body weight of ricin and were sacrified at itntervals of 6, 24, 48 and 120 hours after injectoon. The major morphological changes, such as cloudy swelling, hydropic degeneration, necrosis, fatty change, blood congestion, increase of Kupffer cells in number and extension of sinusoids, were obvious in the liver of experimental group. These morphological changes of hepatic cells were mainly observed in both the periportal and midlobular region of hepatic lobule. The extension of sinusoids was obvious in the controlobular region. And glycogen dlstrlbution of hepatic cells tended to decrease in the same region showing morphlogical changes as compared with the control group.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.1
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• pp.105-112
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• 2003

This experimental study was designed to investigate the effects of Seunggijoui-tang on the weight of obese rats induced by high fat diet. A series of experiments have been conducted in order to measure the effects of above the body weight, serum and fat cell. The measurements have been performed on; (1) the increasing amount of body weight; (2) the quantity of total cholesterol, triglyceride, free fatty acid, total lipid, phospholipid, HDL-cholesterol and LDL-cholesterol in serum; (3) the size of the epididymal fat cell and the fat drops area in hepatic lobule. In the SG I (Group fed high fat diet and administered 42.5mg/100g extract of Seunggijoui-tang during 8 weeks), the weight decreased significantly throughout the whole research period. In the SG I & SG II (Group fed high fat diet and administered 85mg/100g extract of Seunggijoui-tang during 8 weeks), the serum total cholesterol, triglyceride, total lipid, phospholipid, LDL-cholesterol decreased significantly in comparison with control group. In the SG I, the serum free fatty acid decreased significantly in comparison with control group. In the SG I & SG II, the serum HDL-cholesterol decreased in comparison with control group, but this result showed no efficacy. In the SG I, the epididymal fat cell decreased significantly in comparison with control group. In the SG II, the fat drops area in hepatic lobule of rats decreased significantly in comparison with control group. Based on the above result, it is assumed that the clinical application of Seunggijoui-tang can help the treatment of obesity.

• The Journal of Korea CHUNA Manual Medicine
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• v.3 no.1
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• pp.15-29
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• 2002

Objectives : This experimental study was designed to investigate the effects of Galgunseunggi-tang on the weight of obese rats Induced by high fat diet. Methods : A series of have been conducted in order to measure the effects of the above materials weight. serum and fat cells. The have been performed on; (1) the Increasing amount of body weight; (2) the quantity of total cholesterol, tryglyceride, total lipid, free fatty acid, phospholipid, HDL -cholesterol and LDL -cholesterol in serum; (3) the average size of epididymal fat cells and the area rate of fat drops in hepatic lobule. Results : The results were summarized as follows: 1. It was proved that Galgunseunggi-tang suppressed the increasing amount of body weight. GS II group (supplied 60mg per 100g) showed a significant value. 2. It was proved that Galgunseunggi-tang improved the total cholesterol, HDL -cholesterol In serum. but did not show a significance. 3. It was proved that Galgunseunggi-tang decreased the quantify of free fatty acid, phospholipid, LDL -cholesterol in serum. GS I group (supplied 30mg per 100g) showed a significant value. 4. It was proved that Galgunseunggi-tang decreased the quantity of triglyceride, total lipid in serum, and GS II group showed a significant value. 5. Galgunseunggi-tang decreased the average size of epididymal fat cells and the area rate of fat drops In hepatic lobule, and GS I group showed a significant value. Conclusions : Based on the above result, it is assumed that the clinical application of Galgunseunggi-tang can help the treatment of obesity.

• The Journal of Internal Korean Medicine
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• v.29 no.2
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• pp.375-384
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• 2008

Objective : This study was carried out to investigate the effects of oral administration of Hyeungbangjihwang-tang (HBJHT) on the liver cirrhosis of rats induced by Carbon tetrachloride for 10 weeks. Method : The histopathological changes were observed. The HBJHT extracts were daily dosed at 50, 100 and 200mg/kg for 12 weeks. Results : Severe hepatocellular necrosis and ballooning, hyperplasia of connective tissue, subdued reduction of hepatic lobule, and hyperplasia of bile duct in portal triad were dramatically decreased in the HBJHT-treated group compared to that of the Carbon tetrachloride-treated control group in histopathological observation. The diameter of hepatic lobules was significantly enlarged in the HBJHT-treated group compared to that of the Carbon tetrachloride-treated control group, and the amounts of connective tissue, degenerative cells and bile ducts were significantly and dose-dependently decreased. Conclusion : It is concluded that HBJHT has a significant recovering effect on the liver of rats induced by carbon tetrachloride.

• Korean Journal of Veterinary Research
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• v.1 no.1
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• pp.65-70
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• 1961

Many losses in domestic animals have bern reported in this country, since sodium monofluoroacetate (Compound 1080) was used as a rodenticide. Lesions described inclubed multiple petechiae in epi- and endocardium, lung and intestinal mucosa, or superficial necrosis of the gastric mucosa. In this studies as to the poisoning 7 rabbits were administered 0.6mg (Group I), 0.4mg (Group II), and 0.3mg (Group III) of sodium monofluoroacetate per kilogram of body weight. and the results obtained were as follows: In addition to the changes mentioned above fatty degeneration in central parts of hepatic lobule or nut meg liver, haemorrhagic feci in cerebral cortex and leptomeninges and fatty degeneration in kidney and cardiac muscles were found.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.8
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• pp.1096-1101
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• 2012

Intrauterine growth retardation (IUGR) leads to the dysfunction in digestive system, as well as the alteration in the expression of some functional proteins. Heat shock protein 70 (Hsp70) could be induced by various stress factors, but whether Hsp70 expression is changed in neonatal IUGR infants has not been demonstrated. This study was conducted to explore the expression of Hsp70 in the liver by using the IUGR piglet model. Liver and plasma samples were obtained from IUGR and normal birth weight (NBW) piglets at birth. The neonatal IUGR piglets had significantly lower liver weight than their counterparts. The activities of aspartate aminotransferase and alanine aminotransferase in serum were enhanced significantly in IUGR indicating liver dysfunction. The activities of superoxide dismutase (p<0.01), glutathione peroxidase (p<0.01) and catalase (p>0.05) were lower and the level of malondialdehybe was higher (p<0.05) in IUGR liver compared with in NBW. According to the results of histological tests, fatty hepatic infiltrates and cytoplasmic vacuolization were present in the liver of IUGR piglets, but not in NBW liver. The expression of Hsp70 protein was significantly higher (p<0.05) in IUGR piglet liver than in NBW. Similar to where the hepatic injuries were observed, location of Hsp70 was mostly in the midzonal hepatic lobule indicating that oxidative stress might be responsible for the increased expression of Hsp70.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.305-306
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• 2003

To treat fulminant hepatic failure (FHF) patients, various extracorporeal bioartificial liver (BAL) systems have been developed. Several requirements should be met for the development of BAL systems: hepatocytes should be cultured in a sufficiently high density; their metabolic functions should be of a sufficiently high level and duration; and the BAL systems module should permit scaling-up and aseptic handling. Several investigators have found that freshly isolated primary hepatocytes can be cultured into three dimensional, tightly packed, freely suspended, multicellular aggregates, or spheroids. These specialized cell structures exhibited enhanced liver specific functions and a prolonged differentiated state compared to cells maintained in a monolayer culture. Cells in spheroids appear to mimic the morphology and ultrastructure of the in vivo liver lobule. The ability of hepatocytes to organize into three-dimensional structures was hypothesized to contribute to their enhanced liver-specific activities. In this study, the ammonia removal rate and urea secretion rate of pig hepatocytes spheroids encapsulated in Ca-alginate bead were determined. A packed-bed bioreactor with encapsulated pig hepatocytes was devised as BAL support system. The efficacy of the system was evaluated in vitro.

• The Journal of Korean Medicine
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• v.21 no.3
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• pp.31-39
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• 2000

Objectives : Chekamuiyiin-tang has effects of promoting diuresis, promoting blood circulation, elimating phlegm and strengthening the body's resistance to disease. We experimented with Chekamuiyiin-tang in order to find effects which might possibly cure obesity. Methods : We fed a control group of rats a high fat diet and administered normal saline for 8 weeks. And we fed an experimental group of rats a high fat diet and administered an extract of Chekamuiyiin-tang for 8weeks. Results : The serum total cholesterol level, triglyceride level, free fatty acid level, total lipid level and phospholipid level in the Chekamuiyiin-tang group showed a significant decrease in comparison with the control group. The serum HDL-cholesterol level in the Chekamuiyiin-tang group showed no change in comparison with the control group. The serum LDL-cholesterol level in the Chekamuiyiin-tang group showed a decrease in comparison with the control group, but this result showed no efficacy. The size of the epididymal fat cell and the fat drops area in the hepatic lobule of rats in the Chekamuiyiin-tang group showed a significant decrease in comparison with the control group. Conclusios : According to the above results, Chekamuiyiin-tang has shown to be capable of curing and preventing obesity.