• Journal of Ginseng Research
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• v.9 no.2
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• pp.135-145
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• 1985

The present study was undertaken in order to elucidate the effect of ginseng butanol fraction on ethanol induced hepatic aniline hydroxylase activity in rat. Ginseng butanol fraction increased the hepatic aniline hydroxylase activity which is inhibited by ethanol addition in the enzyme assay system, whereas not shown the ginseng effect in ethanol absence condition in vitro. It was found that ginseng butanol fraction improved the affinity of aniline hydroxylase under presence of ethanol in the reaction mixture. On the contrary ginseng butanol fraction showed significant decreasing effect on aniline hydroxylase activity induced by ethanol administration. These results suggest that ginseng butanol fraction regulate the hepatic aniline hydroxylase activity which is induced by ethanol consumption.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.1
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• pp.93-100
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• 1989

The effect of each azalea(Rhodoendron mucronulatum) pollen extract on phase I enzyme(aniline hydroxylase)was studied in this experiment. Serum aminotransferases were not changed in mice injected each azalea pollen extract, respectively compared to control group. The hepatic micros mal aniline hydroxylase activities in the presence of each azalea pollen extract were not affected in vitro. After treatment with azalea pollen water extract, hepatic microsomal aniline hydroxylase activity was increased with dose-dependent manner as compared to control group. The increment of hepatic microsmal aniline hydroxylase activity was more powerful by the treatment of water extract. As mice received aniline after pollen butanol and water extract-pretreatment once a day for 5 days, the blood and liver levels of aniline were decreased significantly.

• Archives of Pharmacal Research
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• v.9 no.2
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• pp.81-86
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• 1986

The effect of imperatorin on hepatic microsomal mixed function oxidases (MF0) was investigated. On acute treatment, imperatorin (30 mg/kg, i.p) caused a significant reduction in activities of hepatic aminopyrine N-demethylase, hexobarbital hydroxylase and aniline hydroxylase as well as cytochrome p0450 content in rats and mice. Kinetic studies on rat liver enzymes revealed that imperatorin appeared to be a competitive inhibitor of aminopyrine N-demethylase (Ki,0.007 mM), whereas a non-competitive inhibitor of hexobarbital hydroxylase (Ki, 0.0148 mM). Imperatorin also inhibited non-competitively aniline metabolism (Ki 0.2 mM). Imperatorin binds to phenobarbital-induced cytochrome p-450 to give a typical type 1 binding sepctrum (max. 388nm, min 422 nm). Multiple administrations of imperatorin (30 mg/kg. i. p. daily for 7 days) to mice shortended markedly the duration of hexobarbital narcosis and increased activities of hepatic aminopyrine N-demethylase and hexobarbital hydroxylase and the level of cytochrome p-450 where as aniline hydroxylase activity was unaffected.

• Preventive Nutrition and Food Science
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• v.13 no.2
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• pp.61-65
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• 2008

To evaluate the effect of ground cherry extract on the activity of aniline hydroxylase, we gave ground cherry extract in doses of 100, 200 or 400 mg/kg i.p to mice for 1, 2 or 4 days. The aniline hydroxylase activity in the group treated with ground cherry extract increased in a dose dependant manner in all experimental groups compared with the control group, and was significantly higher in the group treated with ground cherry extract at a dose of 200 mg/kg, which also exhibited a time dependant increase over 4 days. Enzyzme kinetic analysis was performed for hepatic aniline hydroxylase activity in the group treated with 200 mg/kg for 4 days. There was no change of the Km values for aniline hydroxylase between the experimental group and the control group, but the Vmax values for aniline hydroxylase was 21% lower in the experimental group compared with the control. The experimental group also showed lower lipid peroxide and reduced glutathione content, and there were no significant difference in serum alanine aminotransferase activity between the experimental group and the control. Aniline was injected into both the experimental group mice treated with ground cherry extract at a dose of 200 mg/kg for 4 days and the control group, and then the level of blood aniline was assayed at 1hr. The level of blood aniline was lower in the experimental than the control group. This study suggests that ground cherry extract induces hepatic aniline hydroxylase activity and might accelerate the scavenging system of reactive oxygen species. It is likely that ground cherry extract influences the metabolism of xenobiotics by activating AH activity substituted for CYP2E1.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.3
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• pp.239-246
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• 1989

Preventive effect of azalea pollen extracts against aniline-induced hepatic toxicity in mice was investigated in this experiment. When the biochemical and histological changes were measured, preventive effect was more striking by treatment with water extract. After treatment with azalea pollen extracts, hepatic microsomal aniline hydroxylase activity increased as compared to control. Whereas, aniline level in serum and liver significantly decreased. The Vmax value without affecting Km value increased by the water extract treatment, the results obtained suggest that the characteristics of increase in the aniline hydroxylase activity may include induction of enzyme proteins. These data indicate that the observed preventive effects of azalea pollen extracts against hepatotoxicity is due to the induction of aniline metabolizing enzyme.

• Journal of Environmental Health Sciences
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• v.29 no.2
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• pp.23-28
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• 2003

To evaluate the effect of cyclohexane(CH) treatment on the liver cytochrome P-450 dependent aniline hydroxylase(CYPdAH) activity in alcohol-pretreated animals, CH(1.56 g/kg body weight) was intraperitoneally administered to Sprague-Dawley male rats, which had been drunk 15％ alcohol in distilled water for 1,3 and 6 weeks. CH was injected to rats 4 times every other day and the animals were sacrificed at 24 hours after injection of CH. In the alcohol-pretreated rats, liver injuries were not demonstrated on the basis of the liver weight per body weight, the levels of serum alanine aminotransferase and hepatic microsomal glucose-6-phosphatase activities. By the CH treatment, alcohol-pretreated animals showed the significantly increased activity of hepatic microsomal CYPdAH. Concomitantly $V_{max}$ value in CYPdAH was more increased, whereas $K_{M}$ value more decreased in alcohol-pretreated animals by the treatment of CH. In conclusion, the increasing cause of microsomal CYPdAH in CH-treated rats pretreated with alcohol may be due to induction of enzyme protein in rat liver.r.r.

• Proceedings of the Ginseng society Conference
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• 1988.08a
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• pp.81-86
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• 1988

The effect of three polyacetylene compounds. panaxydol. panaxynol and panaxytriol isolated from Korean ginseng on $CCI_4-induced$ lipid peroxidation in vitro and in vivo hepatic microsomal lipid peroxidation were investigated. Lipid peroxide levels both in serum and liver and serum enzyme (GOT. GPT. LDH) activities of normal or $CCI_4-treated$ mice and rats were also determined after administration of polyacetylenes. Hepatic microsomal cytochrome P-450 content and activities of aniline hydroxylase and aminopyrine demethylase were measured after treatment of polyacetylenes with or without carbon tetrachloride. As results. treatment with polyacetylenes to control mice did not influence the levels of lipid peroxides and serum enzyme activities while panaxynol did. Panaxynol itself inhibited liver lipid peroxidation in normal mice. Polyacetylene compounds protected hepatic lipid peroxidation and lowered serum lipid peroxide levels induced by $CCI_4$ Polyacetylenes prevented leakage of LDH to serum but elevated GOT and GPT levels caused by $CCI_4$ were not changed by polyacetylene pretreatment. $CCI_4$ caused losses in the content of cytochrome P-450 and activities of aniline hydroxylase and aminopyrine demethylase. When polyacetylenes were treated without $CCI_4$ panaxydol and panaxynol induced aniline hydroxylase and all three polyacetylenes induced aminopyrine demethylase. Cytochrome P-450 contents were not affected by polyacetylenes. In vitro hepatic microsomal lipid peroxidation was inhibited by polyacetylenes and $DL-{\alpha}-tocopherol$ in a concentration-dependent manner.

• Toxicological Research
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• v.11 no.2
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• pp.253-259
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• 1995

To investigate an effect of ethanol pretreatment on the bromobenzene metabolism, the brornobenzene (400 mg/kg body wt. i. p.) was given 3 times at intervals of one day to the male rats orally pretreated with 5% ethanol throughout 2 months. In the ethanol pretreated rats, liver injuries were not demonstrated on the basis of the liver weight per body weight, serum levels of alanine aminotransferase (ALT) activity and histopathologic findings. By the bromobenzene treatment, ethanol pretreated rats showed the more decreased levels of serum ALT and liver weight/body weight(%), and decreased degree of liver damage on histopathological observation than the control group. The ethanol pretreated rats showed the more increased activities of hepatic aniline hydroxylase, glutathione Stransf erase (GST) and the more decreased contents of glutathione than the control. Concomitantly the ethanol pretreated rats showed the more decreased contents of hepatic glutathione and increased activities of GST by the bromobenzene treatment. Above results indicate that ethanol pretreatment enhance the metabolizing ability of bromobenzene in rats.

• Toxicological Research
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• v.12 no.2
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• pp.265-270
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• 1996

Effects of a single administration of dichloromethane (DCM) on the hepatic drug metabollzing activity were determined using adult female rats. Rats were treated with DCM (3 mmol/kg, ip) and the disappearance of antipyrine (100 mg/kg, iv) or ethanol (2 g/kg, ip) from blood was measured. The blood concentration and half-life of antipyrine was not influenced by DCM administration. And DCM did not alter the blood concentration of ethanol measured for 240 min after the treatment. The effect of DCM treatment on in vitro cytochrome P-450-dependent enzyme activities was examined as well. No significant difference in either aniline hydroxylase or aminopyrine N-demethylase was observed in hepatic microsomal fractiorts of rats treated with DCM 24 hr prior to sacrifice. The present study indicates that acutely given DCM does not alter the metabolism of xenobiotics in vivo. The failure of DCM to alter the in vitro hepatic microsomal drug metabolizing activity was also noted.

• Toxicological Research
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• v.2 no.1
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• pp.31-36
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• 1986

The present work was undertaken to investigate the effect of glycyrrhetinic acid on pyridine toxicity. When glycyrrhetinic acid was pretreated, pyridine-induced CNS depression and mortality were decreased. A striking enhancement of serum transaminase activities after pyridine administration was markedly decreased by glycyrrhetinic acid pretreatment. It was also observed that the hepatic microsomal aniline hydroxylase activity, which is concerned with pyridine metabolism, was significantly increased in glycyrrhetinic acid pretreated mice.